Amino acid dipepetide frequency for Natranaerovirga hydrolytica

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.306AlaAla: 3.306 ± 0.068
0.543AlaCys: 0.543 ± 0.028
2.498AlaAsp: 2.498 ± 0.058
3.026AlaGlu: 3.026 ± 0.073
2.801AlaPhe: 2.801 ± 0.064
3.673AlaGly: 3.673 ± 0.082
0.968AlaHis: 0.968 ± 0.031
6.078AlaIle: 6.078 ± 0.089
4.061AlaLys: 4.061 ± 0.085
6.467AlaLeu: 6.467 ± 0.091
1.658AlaMet: 1.658 ± 0.047
2.85AlaAsn: 2.85 ± 0.07
1.43AlaPro: 1.43 ± 0.041
1.926AlaGln: 1.926 ± 0.046
1.832AlaArg: 1.832 ± 0.05
3.329AlaSer: 3.329 ± 0.071
3.197AlaThr: 3.197 ± 0.167
3.802AlaVal: 3.802 ± 0.072
0.348AlaTrp: 0.348 ± 0.021
2.538AlaTyr: 2.538 ± 0.059
0.0AlaXaa: 0.0 ± 0.0
Cys
0.449CysAla: 0.449 ± 0.022
0.133CysCys: 0.133 ± 0.013
0.478CysAsp: 0.478 ± 0.024
0.571CysGlu: 0.571 ± 0.031
0.354CysPhe: 0.354 ± 0.019
0.747CysGly: 0.747 ± 0.035
0.232CysHis: 0.232 ± 0.018
0.873CysIle: 0.873 ± 0.031
0.676CysLys: 0.676 ± 0.027
0.711CysLeu: 0.711 ± 0.029
0.233CysMet: 0.233 ± 0.017
0.488CysAsn: 0.488 ± 0.025
0.424CysPro: 0.424 ± 0.025
0.299CysGln: 0.299 ± 0.021
0.26CysArg: 0.26 ± 0.017
0.576CysSer: 0.576 ± 0.031
0.439CysThr: 0.439 ± 0.022
0.55CysVal: 0.55 ± 0.028
0.049CysTrp: 0.049 ± 0.008
0.358CysTyr: 0.358 ± 0.02
0.0CysXaa: 0.0 ± 0.0
Asp
2.758AspAla: 2.758 ± 0.055
0.498AspCys: 0.498 ± 0.027
2.847AspAsp: 2.847 ± 0.072
4.692AspGlu: 4.692 ± 0.07
2.788AspPhe: 2.788 ± 0.063
3.148AspGly: 3.148 ± 0.08
1.048AspHis: 1.048 ± 0.037
6.046AspIle: 6.046 ± 0.097
3.957AspLys: 3.957 ± 0.068
5.254AspLeu: 5.254 ± 0.082
1.451AspMet: 1.451 ± 0.039
3.073AspAsn: 3.073 ± 0.071
1.601AspPro: 1.601 ± 0.046
1.664AspGln: 1.664 ± 0.042
1.755AspArg: 1.755 ± 0.05
2.693AspSer: 2.693 ± 0.06
2.972AspThr: 2.972 ± 0.068
3.69AspVal: 3.69 ± 0.063
0.442AspTrp: 0.442 ± 0.026
2.889AspTyr: 2.889 ± 0.059
0.0AspXaa: 0.0 ± 0.0
Glu
4.815GluAla: 4.815 ± 0.089
0.474GluCys: 0.474 ± 0.024
5.031GluAsp: 5.031 ± 0.091
7.613GluGlu: 7.613 ± 0.137
2.498GluPhe: 2.498 ± 0.053
4.591GluGly: 4.591 ± 0.084
1.286GluHis: 1.286 ± 0.043
6.804GluIle: 6.804 ± 0.102
7.361GluLys: 7.361 ± 0.125
6.685GluLeu: 6.685 ± 0.104
2.217GluMet: 2.217 ± 0.05
4.431GluAsn: 4.431 ± 0.076
1.783GluPro: 1.783 ± 0.051
2.856GluGln: 2.856 ± 0.067
2.664GluArg: 2.664 ± 0.064
3.817GluSer: 3.817 ± 0.074
3.775GluThr: 3.775 ± 0.082
5.246GluVal: 5.246 ± 0.087
0.578GluTrp: 0.578 ± 0.03
3.126GluTyr: 3.126 ± 0.068
0.0GluXaa: 0.0 ± 0.0
Phe
2.397PheAla: 2.397 ± 0.06
0.4PheCys: 0.4 ± 0.021
2.484PheAsp: 2.484 ± 0.058
2.992PheGlu: 2.992 ± 0.067
2.194PhePhe: 2.194 ± 0.064
2.807PheGly: 2.807 ± 0.06
0.634PheHis: 0.634 ± 0.029
4.191PheIle: 4.191 ± 0.087
2.715PheLys: 2.715 ± 0.062
4.016PheLeu: 4.016 ± 0.084
1.12PheMet: 1.12 ± 0.035
2.594PheAsn: 2.594 ± 0.06
1.165PhePro: 1.165 ± 0.043
1.161PheGln: 1.161 ± 0.038
1.255PheArg: 1.255 ± 0.041
3.021PheSer: 3.021 ± 0.06
2.103PheThr: 2.103 ± 0.046
3.111PheVal: 3.111 ± 0.062
0.337PheTrp: 0.337 ± 0.02
1.801PheTyr: 1.801 ± 0.046
0.0PheXaa: 0.0 ± 0.0
Gly
4.054GlyAla: 4.054 ± 0.187
0.734GlyCys: 0.734 ± 0.033
3.077GlyAsp: 3.077 ± 0.062
4.083GlyGlu: 4.083 ± 0.077
3.009GlyPhe: 3.009 ± 0.061
3.872GlyGly: 3.872 ± 0.077
1.253GlyHis: 1.253 ± 0.034
6.481GlyIle: 6.481 ± 0.101
4.371GlyLys: 4.371 ± 0.095
6.074GlyLeu: 6.074 ± 0.102
1.867GlyMet: 1.867 ± 0.053
3.01GlyAsn: 3.01 ± 0.075
1.313GlyPro: 1.313 ± 0.058
1.955GlyGln: 1.955 ± 0.059
2.194GlyArg: 2.194 ± 0.062
3.612GlySer: 3.612 ± 0.071
3.539GlyThr: 3.539 ± 0.064
4.444GlyVal: 4.444 ± 0.083
0.539GlyTrp: 0.539 ± 0.026
3.112GlyTyr: 3.112 ± 0.065
0.0GlyXaa: 0.0 ± 0.0
His
0.726HisAla: 0.726 ± 0.029
0.22HisCys: 0.22 ± 0.016
0.687HisAsp: 0.687 ± 0.033
0.747HisGlu: 0.747 ± 0.028
0.848HisPhe: 0.848 ± 0.031
0.952HisGly: 0.952 ± 0.031
0.474HisHis: 0.474 ± 0.027
1.9HisIle: 1.9 ± 0.059
1.346HisLys: 1.346 ± 0.044
1.812HisLeu: 1.812 ± 0.054
0.474HisMet: 0.474 ± 0.024
1.11HisAsn: 1.11 ± 0.041
0.842HisPro: 0.842 ± 0.034
0.72HisGln: 0.72 ± 0.03
0.653HisArg: 0.653 ± 0.026
1.194HisSer: 1.194 ± 0.032
1.147HisThr: 1.147 ± 0.034
0.683HisVal: 0.683 ± 0.029
0.165HisTrp: 0.165 ± 0.016
1.003HisTyr: 1.003 ± 0.039
0.0HisXaa: 0.0 ± 0.0
Ile
5.758IleAla: 5.758 ± 0.083
0.889IleCys: 0.889 ± 0.033
5.605IleAsp: 5.605 ± 0.093
7.696IleGlu: 7.696 ± 0.122
3.759IlePhe: 3.759 ± 0.089
6.424IleGly: 6.424 ± 0.103
1.752IleHis: 1.752 ± 0.042
9.632IleIle: 9.632 ± 0.166
7.758IleLys: 7.758 ± 0.125
8.556IleLeu: 8.556 ± 0.121
2.471IleMet: 2.471 ± 0.065
5.874IleAsn: 5.874 ± 0.102
3.235IlePro: 3.235 ± 0.069
3.394IleGln: 3.394 ± 0.069
3.266IleArg: 3.266 ± 0.063
5.753IleSer: 5.753 ± 0.105
5.398IleThr: 5.398 ± 0.086
6.026IleVal: 6.026 ± 0.089
0.556IleTrp: 0.556 ± 0.027
3.4IleTyr: 3.4 ± 0.07
0.0IleXaa: 0.0 ± 0.0
Lys
4.654LysAla: 4.654 ± 0.089
0.55LysCys: 0.55 ± 0.027
5.086LysAsp: 5.086 ± 0.089
8.528LysGlu: 8.528 ± 0.149
1.991LysPhe: 1.991 ± 0.045
4.582LysGly: 4.582 ± 0.093
1.248LysHis: 1.248 ± 0.039
6.592LysIle: 6.592 ± 0.093
7.606LysLys: 7.606 ± 0.137
5.785LysLeu: 5.785 ± 0.093
2.117LysMet: 2.117 ± 0.048
4.982LysAsn: 4.982 ± 0.095
1.948LysPro: 1.948 ± 0.058
2.428LysGln: 2.428 ± 0.051
2.857LysArg: 2.857 ± 0.063
4.049LysSer: 4.049 ± 0.072
3.99LysThr: 3.99 ± 0.078
5.25LysVal: 5.25 ± 0.096
0.499LysTrp: 0.499 ± 0.026
2.908LysTyr: 2.908 ± 0.056
0.0LysXaa: 0.0 ± 0.0
Leu
5.243LeuAla: 5.243 ± 0.082
0.861LeuCys: 0.861 ± 0.03
5.199LeuAsp: 5.199 ± 0.069
7.616LeuGlu: 7.616 ± 0.118
4.17LeuPhe: 4.17 ± 0.094
5.945LeuGly: 5.945 ± 0.106
1.257LeuHis: 1.257 ± 0.038
8.434LeuIle: 8.434 ± 0.118
7.389LeuLys: 7.389 ± 0.113
9.153LeuLeu: 9.153 ± 0.143
2.737LeuMet: 2.737 ± 0.063
5.374LeuAsn: 5.374 ± 0.103
2.972LeuPro: 2.972 ± 0.054
2.566LeuGln: 2.566 ± 0.056
3.11LeuArg: 3.11 ± 0.064
6.723LeuSer: 6.723 ± 0.098
4.97LeuThr: 4.97 ± 0.081
5.586LeuVal: 5.586 ± 0.092
0.646LeuTrp: 0.646 ± 0.029
3.56LeuTyr: 3.56 ± 0.07
0.001LeuXaa: 0.001 ± 0.001
Met
1.793MetAla: 1.793 ± 0.053
0.204MetCys: 0.204 ± 0.016
1.768MetAsp: 1.768 ± 0.047
1.876MetGlu: 1.876 ± 0.046
1.004MetPhe: 1.004 ± 0.037
1.762MetGly: 1.762 ± 0.05
0.41MetHis: 0.41 ± 0.022
2.433MetIle: 2.433 ± 0.062
2.264MetLys: 2.264 ± 0.053
2.356MetLeu: 2.356 ± 0.057
0.764MetMet: 0.764 ± 0.031
1.779MetAsn: 1.779 ± 0.038
1.052MetPro: 1.052 ± 0.028
0.763MetGln: 0.763 ± 0.031
0.839MetArg: 0.839 ± 0.035
1.718MetSer: 1.718 ± 0.046
1.412MetThr: 1.412 ± 0.035
1.868MetVal: 1.868 ± 0.048
0.158MetTrp: 0.158 ± 0.014
0.94MetTyr: 0.94 ± 0.034
0.0MetXaa: 0.0 ± 0.0
Asn
3.097AsnAla: 3.097 ± 0.067
0.461AsnCys: 0.461 ± 0.024
3.018AsnAsp: 3.018 ± 0.066
4.677AsnGlu: 4.677 ± 0.084
2.02AsnPhe: 2.02 ± 0.05
3.271AsnGly: 3.271 ± 0.069
1.295AsnHis: 1.295 ± 0.035
5.951AsnIle: 5.951 ± 0.086
4.78AsnLys: 4.78 ± 0.076
4.811AsnLeu: 4.811 ± 0.083
1.556AsnMet: 1.556 ± 0.045
4.169AsnAsn: 4.169 ± 0.082
2.205AsnPro: 2.205 ± 0.054
2.541AsnGln: 2.541 ± 0.06
2.114AsnArg: 2.114 ± 0.054
2.762AsnSer: 2.762 ± 0.07
3.074AsnThr: 3.074 ± 0.072
3.64AsnVal: 3.64 ± 0.076
0.456AsnTrp: 0.456 ± 0.027
2.445AsnTyr: 2.445 ± 0.058
0.0AsnXaa: 0.0 ± 0.0
Pro
1.371ProAla: 1.371 ± 0.043
0.266ProCys: 0.266 ± 0.015
1.524ProAsp: 1.524 ± 0.044
2.384ProGlu: 2.384 ± 0.064
1.473ProPhe: 1.473 ± 0.046
1.575ProGly: 1.575 ± 0.052
0.545ProHis: 0.545 ± 0.029
3.103ProIle: 3.103 ± 0.062
2.008ProLys: 2.008 ± 0.054
2.805ProLeu: 2.805 ± 0.051
0.777ProMet: 0.777 ± 0.029
1.819ProAsn: 1.819 ± 0.053
0.732ProPro: 0.732 ± 0.032
0.939ProGln: 0.939 ± 0.036
0.955ProArg: 0.955 ± 0.032
1.963ProSer: 1.963 ± 0.052
1.801ProThr: 1.801 ± 0.058
2.138ProVal: 2.138 ± 0.06
0.256ProTrp: 0.256 ± 0.018
1.43ProTyr: 1.43 ± 0.04
0.0ProXaa: 0.0 ± 0.0
Gln
1.697GlnAla: 1.697 ± 0.051
0.313GlnCys: 0.313 ± 0.019
1.704GlnAsp: 1.704 ± 0.045
2.854GlnGlu: 2.854 ± 0.068
1.276GlnPhe: 1.276 ± 0.039
1.923GlnGly: 1.923 ± 0.046
0.496GlnHis: 0.496 ± 0.021
2.813GlnIle: 2.813 ± 0.069
2.588GlnLys: 2.588 ± 0.052
3.465GlnLeu: 3.465 ± 0.062
1.014GlnMet: 1.014 ± 0.038
1.768GlnAsn: 1.768 ± 0.055
0.853GlnPro: 0.853 ± 0.037
1.106GlnGln: 1.106 ± 0.039
1.215GlnArg: 1.215 ± 0.037
2.097GlnSer: 2.097 ± 0.049
1.669GlnThr: 1.669 ± 0.052
2.041GlnVal: 2.041 ± 0.052
0.362GlnTrp: 0.362 ± 0.022
1.623GlnTyr: 1.623 ± 0.044
0.0GlnXaa: 0.0 ± 0.0
Arg
1.794ArgAla: 1.794 ± 0.054
0.326ArgCys: 0.326 ± 0.022
1.815ArgAsp: 1.815 ± 0.051
2.68ArgGlu: 2.68 ± 0.074
1.543ArgPhe: 1.543 ± 0.04
1.913ArgGly: 1.913 ± 0.053
0.592ArgHis: 0.592 ± 0.023
3.209ArgIle: 3.209 ± 0.072
2.716ArgLys: 2.716 ± 0.058
3.217ArgLeu: 3.217 ± 0.065
1.018ArgMet: 1.018 ± 0.033
1.945ArgAsn: 1.945 ± 0.051
1.002ArgPro: 1.002 ± 0.035
1.107ArgGln: 1.107 ± 0.04
1.411ArgArg: 1.411 ± 0.045
1.819ArgSer: 1.819 ± 0.045
1.807ArgThr: 1.807 ± 0.05
2.358ArgVal: 2.358 ± 0.057
0.281ArgTrp: 0.281 ± 0.018
1.574ArgTyr: 1.574 ± 0.049
0.0ArgXaa: 0.0 ± 0.0
Ser
2.978SerAla: 2.978 ± 0.057
0.491SerCys: 0.491 ± 0.025
2.979SerAsp: 2.979 ± 0.069
3.817SerGlu: 3.817 ± 0.079
2.81SerPhe: 2.81 ± 0.066
4.076SerGly: 4.076 ± 0.075
1.107SerHis: 1.107 ± 0.036
6.241SerIle: 6.241 ± 0.103
4.343SerLys: 4.343 ± 0.08
5.552SerLeu: 5.552 ± 0.082
1.556SerMet: 1.556 ± 0.044
3.462SerAsn: 3.462 ± 0.074
1.681SerPro: 1.681 ± 0.048
2.104SerGln: 2.104 ± 0.047
2.168SerArg: 2.168 ± 0.05
3.765SerSer: 3.765 ± 0.081
3.157SerThr: 3.157 ± 0.074
3.806SerVal: 3.806 ± 0.072
0.423SerTrp: 0.423 ± 0.021
2.541SerTyr: 2.541 ± 0.055
0.0SerXaa: 0.0 ± 0.0
Thr
3.007ThrAla: 3.007 ± 0.07
0.401ThrCys: 0.401 ± 0.021
2.586ThrAsp: 2.586 ± 0.067
3.155ThrGlu: 3.155 ± 0.074
2.452ThrPhe: 2.452 ± 0.049
3.92ThrGly: 3.92 ± 0.195
1.1ThrHis: 1.1 ± 0.036
5.528ThrIle: 5.528 ± 0.085
3.539ThrLys: 3.539 ± 0.073
5.696ThrLeu: 5.696 ± 0.079
1.264ThrMet: 1.264 ± 0.036
2.883ThrAsn: 2.883 ± 0.066
2.01ThrPro: 2.01 ± 0.055
1.842ThrGln: 1.842 ± 0.042
1.77ThrArg: 1.77 ± 0.053
3.164ThrSer: 3.164 ± 0.055
3.153ThrThr: 3.153 ± 0.076
3.722ThrVal: 3.722 ± 0.059
0.369ThrTrp: 0.369 ± 0.022
2.26ThrTyr: 2.26 ± 0.087
0.0ThrXaa: 0.0 ± 0.0
Val
3.916ValAla: 3.916 ± 0.073
0.623ValCys: 0.623 ± 0.031
3.799ValAsp: 3.799 ± 0.071
4.94ValGlu: 4.94 ± 0.09
3.178ValPhe: 3.178 ± 0.068
4.295ValGly: 4.295 ± 0.087
1.014ValHis: 1.014 ± 0.032
6.282ValIle: 6.282 ± 0.093
4.568ValLys: 4.568 ± 0.083
6.526ValLeu: 6.526 ± 0.096
1.695ValMet: 1.695 ± 0.044
3.59ValAsn: 3.59 ± 0.061
2.095ValPro: 2.095 ± 0.052
1.806ValGln: 1.806 ± 0.046
1.962ValArg: 1.962 ± 0.056
4.149ValSer: 4.149 ± 0.074
3.492ValThr: 3.492 ± 0.066
4.6ValVal: 4.6 ± 0.084
0.398ValTrp: 0.398 ± 0.023
2.577ValTyr: 2.577 ± 0.056
0.0ValXaa: 0.0 ± 0.0
Trp
0.372TrpAla: 0.372 ± 0.024
0.085TrpCys: 0.085 ± 0.011
0.422TrpAsp: 0.422 ± 0.024
0.526TrpGlu: 0.526 ± 0.023
0.332TrpPhe: 0.332 ± 0.02
0.521TrpGly: 0.521 ± 0.025
0.168TrpHis: 0.168 ± 0.012
0.662TrpIle: 0.662 ± 0.025
0.456TrpLys: 0.456 ± 0.026
0.734TrpLeu: 0.734 ± 0.031
0.221TrpMet: 0.221 ± 0.017
0.365TrpAsn: 0.365 ± 0.026
0.169TrpPro: 0.169 ± 0.015
0.296TrpGln: 0.296 ± 0.02
0.273TrpArg: 0.273 ± 0.02
0.433TrpSer: 0.433 ± 0.026
0.327TrpThr: 0.327 ± 0.02
0.541TrpVal: 0.541 ± 0.026
0.073TrpTrp: 0.073 ± 0.009
0.304TrpTyr: 0.304 ± 0.018
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.145TyrAla: 2.145 ± 0.051
0.426TyrCys: 0.426 ± 0.021
2.658TyrAsp: 2.658 ± 0.101
3.178TyrGlu: 3.178 ± 0.066
1.997TyrPhe: 1.997 ± 0.05
2.597TyrGly: 2.597 ± 0.049
0.826TyrHis: 0.826 ± 0.035
3.951TyrIle: 3.951 ± 0.076
3.153TyrLys: 3.153 ± 0.071
3.836TyrLeu: 3.836 ± 0.064
0.952TyrMet: 0.952 ± 0.035
2.776TyrAsn: 2.776 ± 0.062
1.353TyrPro: 1.353 ± 0.042
1.402TyrGln: 1.402 ± 0.044
1.577TyrArg: 1.577 ± 0.047
2.42TyrSer: 2.42 ± 0.052
2.397TyrThr: 2.397 ± 0.074
2.404TyrVal: 2.404 ± 0.056
0.343TyrTrp: 0.343 ± 0.023
2.259TyrTyr: 2.259 ± 0.068
0.001TyrXaa: 0.001 ± 0.001
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.001XaaIle: 0.001 ± 0.001
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.001XaaGln: 0.001 ± 0.001
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.005XaaXaa: 0.005 ± 0.003
Statistics based on 2725 proteins (858221 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski