Streptococcus satellite phage Javan211
Average proteome isoelectric point is 6.52
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 12 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZLL3|A0A4D5ZLL3_9VIRU Integrase/recombinase OS=Streptococcus satellite phage Javan211 OX=2558571 GN=JavanS211_0001 PE=3 SV=1
MM1 pKa = 7.64 SDD3 pKa = 3.48 KK4 pKa = 10.82 KK5 pKa = 10.91 FNYY8 pKa = 9.8 EE9 pKa = 3.51 NVRR12 pKa = 11.84 AKK14 pKa = 10.97 NFGVWLEE21 pKa = 4.19 EE22 pKa = 4.08 AFQTMLDD29 pKa = 3.95 FSLEE33 pKa = 4.14 NKK35 pKa = 9.55 FDD37 pKa = 3.95 CYY39 pKa = 10.94 SIEE42 pKa = 4.03 EE43 pKa = 4.13 QNQLEE48 pKa = 4.34 RR49 pKa = 11.84 VLEE52 pKa = 4.03 ILTDD56 pKa = 4.25 CFDD59 pKa = 3.54 MWDD62 pKa = 3.45 KK63 pKa = 11.4 GQIILVSKK71 pKa = 10.25 EE72 pKa = 4.29 SEE74 pKa = 4.19 DD75 pKa = 3.59 KK76 pKa = 11.3
Molecular weight: 9.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.48
IPC2_protein 4.38
IPC_protein 4.253
Toseland 4.088
ProMoST 4.368
Dawson 4.202
Bjellqvist 4.355
Wikipedia 4.075
Rodwell 4.088
Grimsley 3.999
Solomon 4.19
Lehninger 4.151
Nozaki 4.317
DTASelect 4.457
Thurlkill 4.101
EMBOSS 4.101
Sillero 4.355
Patrickios 3.897
IPC_peptide 4.202
IPC2_peptide 4.342
IPC2.peptide.svr19 4.296
Protein with the highest isoelectric point:
>tr|A0A4D5ZLM2|A0A4D5ZLM2_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan211 OX=2558571 GN=JavanS211_0011 PE=4 SV=1
MM1 pKa = 6.55 VTVKK5 pKa = 10.36 RR6 pKa = 11.84 LAIEE10 pKa = 5.67 AITKK14 pKa = 9.36 PMKK17 pKa = 10.75 LKK19 pKa = 10.93 GLTKK23 pKa = 10.43 AVARR27 pKa = 11.84 LDD29 pKa = 4.12 GEE31 pKa = 4.08 RR32 pKa = 11.84 LDD34 pKa = 4.32 INLEE38 pKa = 3.77 ALFIEE43 pKa = 5.26 FEE45 pKa = 4.26 SQRR48 pKa = 11.84 LEE50 pKa = 3.89 LDD52 pKa = 3.79 KK53 pKa = 11.19 IAGTKK58 pKa = 9.44 GGYY61 pKa = 9.59 RR62 pKa = 11.84 YY63 pKa = 10.0 FFLCPRR69 pKa = 11.84 CQKK72 pKa = 10.16 RR73 pKa = 11.84 CRR75 pKa = 11.84 VLYY78 pKa = 10.13 KK79 pKa = 10.42 RR80 pKa = 11.84 EE81 pKa = 3.28 IAYY84 pKa = 9.4 YY85 pKa = 10.69 CRR87 pKa = 11.84 MCQGIHH93 pKa = 5.97 KK94 pKa = 8.33 QTLNRR99 pKa = 11.84 SKK101 pKa = 9.84 TDD103 pKa = 3.25 CQYY106 pKa = 11.19 YY107 pKa = 9.02 WEE109 pKa = 4.9 LALKK113 pKa = 9.04 EE114 pKa = 3.98 ARR116 pKa = 11.84 KK117 pKa = 9.3 IDD119 pKa = 3.84 PYY121 pKa = 9.97 FTPKK125 pKa = 10.2 RR126 pKa = 11.84 GGYY129 pKa = 8.64 MFDD132 pKa = 4.25 GFPEE136 pKa = 4.16 RR137 pKa = 11.84 PKK139 pKa = 10.79 YY140 pKa = 10.06 MRR142 pKa = 11.84 INTYY146 pKa = 9.68 LKK148 pKa = 9.27 HH149 pKa = 5.15 YY150 pKa = 10.28 RR151 pKa = 11.84 RR152 pKa = 11.84 FRR154 pKa = 11.84 KK155 pKa = 9.77 YY156 pKa = 10.2 VEE158 pKa = 4.72 KK159 pKa = 10.85 GDD161 pKa = 4.1 SLWLKK166 pKa = 10.6 GFF168 pKa = 3.94
Molecular weight: 20.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.191
IPC2_protein 9.311
IPC_protein 9.297
Toseland 9.838
ProMoST 9.604
Dawson 10.101
Bjellqvist 9.823
Wikipedia 10.277
Rodwell 10.438
Grimsley 10.175
Solomon 10.116
Lehninger 10.072
Nozaki 9.926
DTASelect 9.794
Thurlkill 9.94
EMBOSS 10.262
Sillero 10.028
Patrickios 7.556
IPC_peptide 10.116
IPC2_peptide 8.639
IPC2.peptide.svr19 8.269
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
12
0
12
2046
40
387
170.5
19.77
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.403 ± 0.722
0.88 ± 0.346
5.621 ± 0.682
8.602 ± 1.079
4.008 ± 0.507
4.203 ± 0.567
1.124 ± 0.346
6.696 ± 0.343
10.264 ± 0.625
10.313 ± 0.673
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.955 ± 0.292
6.452 ± 0.712
2.59 ± 0.338
4.301 ± 0.469
4.888 ± 0.725
5.718 ± 0.943
5.523 ± 0.426
4.545 ± 0.6
1.026 ± 0.231
4.888 ± 0.43
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here