Gordonia sp. HNM0687

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Gordoniaceae; Gordonia; unclassified Gordonia

Average proteome isoelectric point is 5.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4687 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6L7GN09|A0A6L7GN09_9ACTN Winged helix DNA-binding protein OS=Gordonia sp. HNM0687 OX=2665643 GN=GIY30_08030 PE=4 SV=1
MM1 pKa = 7.75LIALLSILVLALAGCSNNEE20 pKa = 3.71EE21 pKa = 4.5SEE23 pKa = 4.24GLGADD28 pKa = 3.88ISPTSVDD35 pKa = 3.12VDD37 pKa = 3.94PVAEE41 pKa = 4.23IADD44 pKa = 4.25LVPAPIRR51 pKa = 11.84EE52 pKa = 4.19SGQLVVGVNVPYY64 pKa = 10.68APNEE68 pKa = 4.06FKK70 pKa = 10.77DD71 pKa = 3.53ASGDD75 pKa = 3.64IIGFDD80 pKa = 3.8VDD82 pKa = 4.36LMNAVASVLDD92 pKa = 3.55LTPQYY97 pKa = 10.93RR98 pKa = 11.84EE99 pKa = 3.97AQFDD103 pKa = 4.06TIIPSVQAGTYY114 pKa = 8.51NVGMSSFTDD123 pKa = 3.42TKK125 pKa = 10.73EE126 pKa = 4.22RR127 pKa = 11.84EE128 pKa = 4.2AQVDD132 pKa = 3.75FVTYY136 pKa = 9.91YY137 pKa = 10.52SAGVQWAQQVGAGIDD152 pKa = 3.52PNNACGKK159 pKa = 9.37RR160 pKa = 11.84VAVQSTTYY168 pKa = 10.54EE169 pKa = 4.09DD170 pKa = 3.63TDD172 pKa = 4.05EE173 pKa = 5.05IPAKK177 pKa = 10.61SQACTDD183 pKa = 3.48AGQPAIEE190 pKa = 4.23KK191 pKa = 9.94VRR193 pKa = 11.84YY194 pKa = 9.46DD195 pKa = 3.84SQDD198 pKa = 3.06EE199 pKa = 4.26ATNALMLGRR208 pKa = 11.84VDD210 pKa = 5.49AMSADD215 pKa = 4.07SPVTSYY221 pKa = 11.21AIEE224 pKa = 4.16RR225 pKa = 11.84SNGALEE231 pKa = 4.03PAGGVFDD238 pKa = 4.23SAPYY242 pKa = 9.97GYY244 pKa = 9.07PVQKK248 pKa = 10.57GSQLAQALQQAVQYY262 pKa = 10.89LIDD265 pKa = 3.9NGQYY269 pKa = 10.09EE270 pKa = 5.23AIAEE274 pKa = 4.15HH275 pKa = 6.78WGLGAGTIPTSVINGAVSS293 pKa = 2.95

Molecular weight: 30.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6L7GNH7|A0A6L7GNH7_9ACTN LLM class F420-dependent oxidoreductase OS=Gordonia sp. HNM0687 OX=2665643 GN=GIY30_08860 PE=4 SV=1
MM1 pKa = 7.69AKK3 pKa = 10.06GKK5 pKa = 8.69RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84ARR17 pKa = 11.84VHH19 pKa = 5.99GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AIVNGRR37 pKa = 11.84RR38 pKa = 11.84SKK40 pKa = 10.92GRR42 pKa = 11.84AKK44 pKa = 9.67LTAA47 pKa = 4.21

Molecular weight: 5.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4687

0

4687

1589854

31

8333

339.2

36.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.818 ± 0.045

0.767 ± 0.01

6.931 ± 0.033

5.328 ± 0.026

2.965 ± 0.02

8.889 ± 0.038

2.249 ± 0.017

4.486 ± 0.024

1.938 ± 0.026

9.457 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.097 ± 0.014

2.079 ± 0.02

5.567 ± 0.027

2.783 ± 0.017

7.417 ± 0.04

5.628 ± 0.024

6.331 ± 0.023

8.813 ± 0.036

1.441 ± 0.016

2.017 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski