Psychrobacter arcticus (strain DSM 17307 / VKM B-2377 / 273-4)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Moraxellaceae; Psychrobacter; Psychrobacter arcticus

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2099 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q4FT30|Q4FT30_PSYA2 Glutamine synthetase OS=Psychrobacter arcticus (strain DSM 17307 / VKM B-2377 / 273-4) OX=259536 GN=glnA PE=3 SV=1
MM1 pKa = 7.17AQIIRR6 pKa = 11.84LDD8 pKa = 3.44NDD10 pKa = 3.49ADD12 pKa = 3.88NEE14 pKa = 4.09ASSKK18 pKa = 10.77NGHH21 pKa = 6.77AYY23 pKa = 9.7IIIDD27 pKa = 4.48DD28 pKa = 4.08KK29 pKa = 11.44CVHH32 pKa = 6.53CKK34 pKa = 10.18YY35 pKa = 10.68DD36 pKa = 3.07SDD38 pKa = 3.76YY39 pKa = 11.43RR40 pKa = 11.84EE41 pKa = 4.04CGEE44 pKa = 4.32RR45 pKa = 11.84NEE47 pKa = 4.14YY48 pKa = 9.11WGGHH52 pKa = 5.32LAVEE56 pKa = 4.49GDD58 pKa = 3.16SWIEE62 pKa = 3.7VDD64 pKa = 4.74SVTNLSIVEE73 pKa = 4.05ILDD76 pKa = 3.69IEE78 pKa = 4.45TDD80 pKa = 3.58DD81 pKa = 4.69LLPLGSYY88 pKa = 11.05VMTADD93 pKa = 3.78DD94 pKa = 3.92TLQIIEE100 pKa = 4.66IIKK103 pKa = 10.54DD104 pKa = 4.09FIEE107 pKa = 4.61GNIEE111 pKa = 3.66ASQPANSYY119 pKa = 9.54YY120 pKa = 10.93AAA122 pKa = 4.88

Molecular weight: 13.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q4FPS6|Q4FPS6_PSYA2 Possible DNA processing protein OS=Psychrobacter arcticus (strain DSM 17307 / VKM B-2377 / 273-4) OX=259536 GN=dprA PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVIKK11 pKa = 10.52RR12 pKa = 11.84KK13 pKa = 8.26RR14 pKa = 11.84THH16 pKa = 5.92GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.32KK26 pKa = 10.31GRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.03GRR39 pKa = 11.84HH40 pKa = 5.34RR41 pKa = 11.84LTVV44 pKa = 3.07

Molecular weight: 5.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2099

0

2099

704762

38

6715

335.8

37.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.376 ± 0.087

0.885 ± 0.02

6.088 ± 0.043

5.553 ± 0.055

3.633 ± 0.043

6.642 ± 0.067

2.247 ± 0.032

6.769 ± 0.04

5.121 ± 0.052

9.892 ± 0.077

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.785 ± 0.03

4.55 ± 0.036

3.98 ± 0.028

4.71 ± 0.046

4.444 ± 0.048

6.487 ± 0.046

5.974 ± 0.127

6.698 ± 0.046

1.141 ± 0.02

3.027 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski