Porcine hemagglutinating encephalomyelitis virus (strain 67N) (HEV-67N)

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae; Betacoronavirus; Embecovirus; Betacoronavirus 1; Porcine hemagglutinating encephalomyelitis virus

Average proteome isoelectric point is 6.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q774I1|NS12_CVP67 Non-structural protein of 12.7 kDa OS=Porcine hemagglutinating encephalomyelitis virus (strain 67N) OX=230237 PE=3 SV=1
MM1 pKa = 7.17FMADD5 pKa = 4.32AYY7 pKa = 11.22LADD10 pKa = 3.99TVWYY14 pKa = 8.76VGQIIFIVAICLLVIIVVVAFLATFKK40 pKa = 11.14LCIQLCGMCNTLVLSPSIYY59 pKa = 9.46VFNRR63 pKa = 11.84GRR65 pKa = 11.84QFYY68 pKa = 10.64EE69 pKa = 4.42FYY71 pKa = 11.08NDD73 pKa = 3.24VKK75 pKa = 11.05PPVLDD80 pKa = 3.51VDD82 pKa = 4.53DD83 pKa = 4.05VV84 pKa = 3.68

Molecular weight: 9.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q8BB24|VME1_CVP67 Membrane protein OS=Porcine hemagglutinating encephalomyelitis virus (strain 67N) OX=230237 GN=M PE=3 SV=1
MM1 pKa = 7.55SFTPGKK7 pKa = 9.79QSSSRR12 pKa = 11.84ASSGNRR18 pKa = 11.84SGNGILKK25 pKa = 9.42WADD28 pKa = 3.13QSDD31 pKa = 3.63QSRR34 pKa = 11.84NVQTRR39 pKa = 11.84GRR41 pKa = 11.84RR42 pKa = 11.84VQSKK46 pKa = 7.32QTATSQQPSGGTVVPYY62 pKa = 9.51YY63 pKa = 10.8SWFSGITQFQKK74 pKa = 10.77GKK76 pKa = 9.42EE77 pKa = 3.95FEE79 pKa = 4.16FAEE82 pKa = 4.69GQGVPIAPGVPSTEE96 pKa = 4.06AKK98 pKa = 10.31GYY100 pKa = 8.63WYY102 pKa = 10.34RR103 pKa = 11.84HH104 pKa = 4.43NRR106 pKa = 11.84RR107 pKa = 11.84SFKK110 pKa = 10.37TADD113 pKa = 3.13GNQRR117 pKa = 11.84QLLPRR122 pKa = 11.84WYY124 pKa = 9.8FYY126 pKa = 11.56YY127 pKa = 10.78LGTGPHH133 pKa = 6.97AKK135 pKa = 9.91DD136 pKa = 3.92QYY138 pKa = 10.35GTDD141 pKa = 3.18IDD143 pKa = 4.14GVFWVASNQADD154 pKa = 3.42INTPADD160 pKa = 3.29IVDD163 pKa = 4.43RR164 pKa = 11.84DD165 pKa = 3.85PSSDD169 pKa = 3.16EE170 pKa = 4.8AIPTRR175 pKa = 11.84FPPGTVLPQGYY186 pKa = 9.38YY187 pKa = 9.56IEE189 pKa = 4.86GSGRR193 pKa = 11.84SAPNSRR199 pKa = 11.84STSRR203 pKa = 11.84APNRR207 pKa = 11.84APSAGSRR214 pKa = 11.84SRR216 pKa = 11.84ANSGNRR222 pKa = 11.84TSTPGVTPDD231 pKa = 3.56MADD234 pKa = 3.28QIASLVLAKK243 pKa = 10.41LGKK246 pKa = 10.07DD247 pKa = 2.95ATKK250 pKa = 9.49PQQVTKK256 pKa = 9.36QTAKK260 pKa = 10.33EE261 pKa = 4.03VRR263 pKa = 11.84QKK265 pKa = 10.72ILNKK269 pKa = 9.23PRR271 pKa = 11.84QKK273 pKa = 10.46RR274 pKa = 11.84SPNKK278 pKa = 9.02QCTVQQCFGKK288 pKa = 10.48RR289 pKa = 11.84GPNQNFGGGEE299 pKa = 3.99MLKK302 pKa = 10.67LGTSDD307 pKa = 3.38PQFPILAEE315 pKa = 4.05LAPTAGAFFFGSRR328 pKa = 11.84LEE330 pKa = 4.03LAKK333 pKa = 10.7VQNLSGNPDD342 pKa = 3.48EE343 pKa = 4.7PQKK346 pKa = 11.04DD347 pKa = 4.22VYY349 pKa = 9.8EE350 pKa = 3.97LRR352 pKa = 11.84YY353 pKa = 10.38NGAIRR358 pKa = 11.84FDD360 pKa = 3.73STLSGFEE367 pKa = 4.32TIMKK371 pKa = 9.76VLNQNLNAYY380 pKa = 7.22QHH382 pKa = 6.17QEE384 pKa = 3.85DD385 pKa = 3.9GMMNISPKK393 pKa = 8.82PQRR396 pKa = 11.84QRR398 pKa = 11.84GQKK401 pKa = 9.61NGQVEE406 pKa = 4.3NDD408 pKa = 3.75NVSVAAPKK416 pKa = 10.71SRR418 pKa = 11.84VQQNKK423 pKa = 9.07SRR425 pKa = 11.84EE426 pKa = 4.15LTAEE430 pKa = 4.92DD431 pKa = 3.26ISLLKK436 pKa = 11.01KK437 pKa = 9.61MDD439 pKa = 3.6EE440 pKa = 4.96PYY442 pKa = 10.5TEE444 pKa = 4.35DD445 pKa = 3.07TSEE448 pKa = 3.68II449 pKa = 3.91

Molecular weight: 49.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

0

8

2872

20

1349

359.0

40.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.71 ± 0.51

3.099 ± 0.817

4.735 ± 0.327

3.238 ± 0.512

5.571 ± 0.599

6.755 ± 0.414

1.323 ± 0.231

6.128 ± 0.748

4.074 ± 0.559

9.192 ± 1.265

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.671 ± 0.364

6.685 ± 0.934

4.701 ± 0.684

4.352 ± 0.817

3.969 ± 0.804

8.217 ± 0.397

6.929 ± 0.423

7.173 ± 0.583

1.288 ± 0.236

5.188 ± 0.813
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski