Auraticoccus monumenti
Average proteome isoelectric point is 6.1
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4049 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1G6V003|A0A1G6V003_9ACTN NADPH:quinone reductase OS=Auraticoccus monumenti OX=675864 GN=SAMN04489747_1007 PE=4 SV=1
MM1 pKa = 7.18 VSPRR5 pKa = 11.84 HH6 pKa = 5.5 RR7 pKa = 11.84 LHH9 pKa = 6.01 RR10 pKa = 11.84 TAAALVGGLLVTTTLLAPAAASPLDD35 pKa = 3.61 IPHH38 pKa = 7.27 DD39 pKa = 4.48 FSAGPDD45 pKa = 2.92 GWFSYY50 pKa = 11.31 GPGATTEE57 pKa = 4.16 VTDD60 pKa = 5.41 GEE62 pKa = 4.93 LCTSLPAHH70 pKa = 6.75 SGNPWDD76 pKa = 3.89 VAVAHH81 pKa = 6.1 NAVGLEE87 pKa = 3.65 AGTTYY92 pKa = 11.17 DD93 pKa = 3.35 VAFTASSTAEE103 pKa = 3.56 IAIRR107 pKa = 11.84 AQVGINAEE115 pKa = 4.27 PYY117 pKa = 9.35 PGVHH121 pKa = 6.85 AEE123 pKa = 4.63 DD124 pKa = 3.86 LTITATPEE132 pKa = 3.5 EE133 pKa = 4.61 HH134 pKa = 6.83 SFSFSFTSDD143 pKa = 2.51 VDD145 pKa = 3.66 AEE147 pKa = 4.07 AAEE150 pKa = 4.44 TGGQLSFQIGNQGEE164 pKa = 4.55 PFTFCLDD171 pKa = 3.67 DD172 pKa = 4.27 FSLTEE177 pKa = 4.0 AGATEE182 pKa = 4.23 EE183 pKa = 4.1 QLPALDD189 pKa = 3.9 VPPFFTTGNLTPSTTVEE206 pKa = 4.05 EE207 pKa = 4.85 GVLCTTVPAGTTNTYY222 pKa = 10.77 DD223 pKa = 4.37 AIVGVNGIPVQEE235 pKa = 4.51 GGSYY239 pKa = 9.26 TLSFLASADD248 pKa = 3.61 VASDD252 pKa = 2.95 ITVVVGEE259 pKa = 4.18 NGGDD263 pKa = 3.41 YY264 pKa = 10.58 RR265 pKa = 11.84 QAFSQAVALDD275 pKa = 3.58 SAEE278 pKa = 3.99 PTRR281 pKa = 11.84 YY282 pKa = 9.9 SYY284 pKa = 11.56 EE285 pKa = 4.02 FTSDD289 pKa = 3.21 LAFPASTGDD298 pKa = 3.69 GAPEE302 pKa = 3.96 GQVAFQLGGGAEE314 pKa = 3.95 AFEE317 pKa = 5.07 LCLSEE322 pKa = 4.62 PSLALEE328 pKa = 4.34 VAAGGNLVPNGDD340 pKa = 4.11 FGSGALDD347 pKa = 3.97 PFTAPEE353 pKa = 4.39 GVAVDD358 pKa = 4.53 LSSGAACLTVPDD370 pKa = 4.46 TLGQYY375 pKa = 10.7 DD376 pKa = 4.05 GLVLNGLAVEE386 pKa = 4.78 EE387 pKa = 4.65 GQTYY391 pKa = 8.33 TLTFDD396 pKa = 4.5 ASADD400 pKa = 3.41 PGKK403 pKa = 8.58 TVRR406 pKa = 11.84 ALIGEE411 pKa = 4.26 NGGSYY416 pKa = 9.39 GTVLDD421 pKa = 4.12 EE422 pKa = 5.65 DD423 pKa = 4.08 VALTAEE429 pKa = 4.56 TTGHH433 pKa = 6.59 EE434 pKa = 4.3 LTFVASAGYY443 pKa = 8.49 PAATVDD449 pKa = 3.71 PGDD452 pKa = 4.13 GPFEE456 pKa = 4.27 GQLAFQVGGRR466 pKa = 11.84 GAFDD470 pKa = 3.47 FCIDD474 pKa = 4.75 DD475 pKa = 3.5 ISLVEE480 pKa = 4.8 SDD482 pKa = 4.61 TPPPPYY488 pKa = 10.24 EE489 pKa = 4.66 PEE491 pKa = 3.73 TGPRR495 pKa = 11.84 IRR497 pKa = 11.84 VNQVGYY503 pKa = 10.65 LPDD506 pKa = 4.04 GPKK509 pKa = 9.91 QATLVTDD516 pKa = 3.22 ATEE519 pKa = 4.01 PLEE522 pKa = 4.15 YY523 pKa = 10.23 EE524 pKa = 4.32 VLADD528 pKa = 4.57 GIVVHH533 pKa = 6.7 TGQTVPAGLDD543 pKa = 3.22 EE544 pKa = 4.3 TAGTDD549 pKa = 3.28 VHH551 pKa = 6.77 EE552 pKa = 5.44 LDD554 pKa = 4.31 FSDD557 pKa = 3.89 YY558 pKa = 11.07 RR559 pKa = 11.84 PDD561 pKa = 3.61 VVDD564 pKa = 4.02 ADD566 pKa = 4.07 AVHH569 pKa = 6.93 EE570 pKa = 4.12 IAVDD574 pKa = 3.94 GEE576 pKa = 4.57 VSFEE580 pKa = 3.82 FAVRR584 pKa = 11.84 DD585 pKa = 4.0 DD586 pKa = 5.25 LYY588 pKa = 11.06 QQLRR592 pKa = 11.84 ADD594 pKa = 3.97 ALNYY598 pKa = 8.93 FYY600 pKa = 10.23 PARR603 pKa = 11.84 SGIAIDD609 pKa = 3.79 GGIMDD614 pKa = 4.99 GQPDD618 pKa = 3.68 AGEE621 pKa = 4.04 YY622 pKa = 9.68 TRR624 pKa = 11.84 DD625 pKa = 3.48 AGHH628 pKa = 6.63 VEE630 pKa = 4.7 DD631 pKa = 5.55 PDD633 pKa = 4.02 SSLVADD639 pKa = 3.92 SPNRR643 pKa = 11.84 GDD645 pKa = 3.2 VDD647 pKa = 4.01 VRR649 pKa = 11.84 CLSPEE654 pKa = 4.0 TEE656 pKa = 3.79 GDD658 pKa = 3.24 YY659 pKa = 10.44 WKK661 pKa = 11.26 YY662 pKa = 11.28 GDD664 pKa = 3.85 WSCDD668 pKa = 3.2 YY669 pKa = 10.37 TLDD672 pKa = 4.87 VVGGWYY678 pKa = 10.02 DD679 pKa = 3.33 AGDD682 pKa = 3.49 HH683 pKa = 6.07 GKK685 pKa = 10.25 YY686 pKa = 9.61 VVNGGISVGQVLSTYY701 pKa = 10.28 EE702 pKa = 3.85 RR703 pKa = 11.84 TLYY706 pKa = 10.95 SPTGTGSALGDD717 pKa = 3.45 GSLNIPEE724 pKa = 4.71 SDD726 pKa = 3.44 NGVPDD731 pKa = 4.58 PLDD734 pKa = 3.42 EE735 pKa = 5.14 ARR737 pKa = 11.84 WEE739 pKa = 4.21 LEE741 pKa = 3.57 WMLSMQAPDD750 pKa = 3.96 GAQHH754 pKa = 6.92 AGMVHH759 pKa = 6.53 HH760 pKa = 7.35 KK761 pKa = 10.18 IADD764 pKa = 3.71 VDD766 pKa = 3.8 WTGLPLLPSEE776 pKa = 5.14 DD777 pKa = 3.5 PQEE780 pKa = 4.78 RR781 pKa = 11.84 YY782 pKa = 8.03 LHH784 pKa = 6.3 RR785 pKa = 11.84 PSTAATLNLAAVAAQGARR803 pKa = 11.84 LWAPYY808 pKa = 9.84 DD809 pKa = 3.35 QAFADD814 pKa = 4.24 EE815 pKa = 4.73 LLAAARR821 pKa = 11.84 VAWDD825 pKa = 3.66 AAVATPDD832 pKa = 3.78 LFEE835 pKa = 4.86 PAPNADD841 pKa = 4.23 PSPGSGPYY849 pKa = 10.44 DD850 pKa = 4.07 DD851 pKa = 6.27 DD852 pKa = 3.91 EE853 pKa = 6.05 VGDD856 pKa = 3.83 EE857 pKa = 4.94 FYY859 pKa = 10.43 WAAAEE864 pKa = 4.36 LFLTTGEE871 pKa = 4.18 EE872 pKa = 4.05 AFEE875 pKa = 4.02 EE876 pKa = 4.55 HH877 pKa = 6.81 VLGSPFHH884 pKa = 6.25 TADD887 pKa = 2.6 IWSPSGFSWGSTAALGRR904 pKa = 11.84 MDD906 pKa = 4.73 LATVEE911 pKa = 4.16 SDD913 pKa = 3.54 LPGRR917 pKa = 11.84 DD918 pKa = 3.53 DD919 pKa = 3.33 VRR921 pKa = 11.84 ASVVEE926 pKa = 4.03 GADD929 pKa = 4.6 RR930 pKa = 11.84 YY931 pKa = 10.9 LGWQQAQPFGTAYY944 pKa = 9.71 PGNEE948 pKa = 3.68 GVYY951 pKa = 9.77 EE952 pKa = 4.1 WGSNSAVLNNQVVIATAFDD971 pKa = 3.67 LTSDD975 pKa = 3.41 QVYY978 pKa = 11.11 ADD980 pKa = 4.09 AVLEE984 pKa = 4.21 AMDD987 pKa = 4.04 YY988 pKa = 11.26 LLGRR992 pKa = 11.84 NALNNSYY999 pKa = 8.48 ITGYY1003 pKa = 11.22 GDD1005 pKa = 3.51 VFSDD1009 pKa = 4.0 DD1010 pKa = 3.23 QHH1012 pKa = 7.8 SRR1014 pKa = 11.84 WFAHH1018 pKa = 6.99 SIDD1021 pKa = 4.46 PDD1023 pKa = 3.95 LPNPPRR1029 pKa = 11.84 GSVAGGPNSDD1039 pKa = 3.25 VGTWDD1044 pKa = 3.47 PVISGLYY1051 pKa = 10.2 DD1052 pKa = 3.61 EE1053 pKa = 5.9 EE1054 pKa = 4.93 RR1055 pKa = 11.84 MCAPQRR1061 pKa = 11.84 CYY1063 pKa = 11.08 LDD1065 pKa = 5.99 DD1066 pKa = 3.88 IQSWSTNEE1074 pKa = 3.46 ITVNWNSALSWVASFVADD1092 pKa = 3.56 QQAGDD1097 pKa = 3.73 EE1098 pKa = 4.39 SGAGQVVDD1106 pKa = 4.69 VVDD1109 pKa = 4.31 HH1110 pKa = 6.97 PEE1112 pKa = 3.99 DD1113 pKa = 3.4 MTVEE1117 pKa = 4.07 EE1118 pKa = 5.22 GEE1120 pKa = 4.44 DD1121 pKa = 3.51 AVFTASASGDD1131 pKa = 3.82 PVPDD1135 pKa = 3.68 VQWQRR1140 pKa = 11.84 LVDD1143 pKa = 4.26 GVWTDD1148 pKa = 2.93 VDD1150 pKa = 3.8 GAEE1153 pKa = 4.19 EE1154 pKa = 3.99 EE1155 pKa = 4.77 TFTFEE1160 pKa = 5.72 AALEE1164 pKa = 4.27 DD1165 pKa = 4.38 DD1166 pKa = 4.34 GALVRR1171 pKa = 11.84 AYY1173 pKa = 10.03 FSNGFGGFFTDD1184 pKa = 4.03 PATLSVTDD1192 pKa = 4.13 AEE1194 pKa = 4.51 PRR1196 pKa = 11.84 PTPGPSDD1203 pKa = 3.92 EE1204 pKa = 5.26 PDD1206 pKa = 4.35 DD1207 pKa = 4.22 GTPDD1211 pKa = 4.41 DD1212 pKa = 5.62 GGAPDD1217 pKa = 5.47 DD1218 pKa = 4.62 GASPGAGGLPEE1229 pKa = 5.94 DD1230 pKa = 4.73 GGTPSEE1236 pKa = 4.46 PGAGGGPTQNLSDD1249 pKa = 4.23 TGPSDD1254 pKa = 3.98 LVLPALLTAVLLLAVGSVVVARR1276 pKa = 11.84 GRR1278 pKa = 11.84 RR1279 pKa = 11.84 QQDD1282 pKa = 3.12 EE1283 pKa = 4.41 LGG1285 pKa = 3.65
Molecular weight: 134.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.7
IPC2_protein 3.656
IPC_protein 3.706
Toseland 3.478
ProMoST 3.859
Dawson 3.694
Bjellqvist 3.834
Wikipedia 3.617
Rodwell 3.516
Grimsley 3.376
Solomon 3.694
Lehninger 3.643
Nozaki 3.795
DTASelect 4.037
Thurlkill 3.516
EMBOSS 3.63
Sillero 3.821
Patrickios 0.96
IPC_peptide 3.681
IPC2_peptide 3.795
IPC2.peptide.svr19 3.739
Protein with the highest isoelectric point:
>tr|A0A1G6RNE3|A0A1G6RNE3_9ACTN Uncharacterized protein OS=Auraticoccus monumenti OX=675864 GN=SAMN04489747_0069 PE=4 SV=1
MM1 pKa = 7.4 GSVIKK6 pKa = 10.42 KK7 pKa = 8.47 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.22 RR11 pKa = 11.84 MAKK14 pKa = 9.41 KK15 pKa = 9.99 KK16 pKa = 9.72 HH17 pKa = 5.74 RR18 pKa = 11.84 KK19 pKa = 7.87 LLKK22 pKa = 8.37 RR23 pKa = 11.84 TRR25 pKa = 11.84 IQRR28 pKa = 11.84 RR29 pKa = 11.84 RR30 pKa = 11.84 AGKK33 pKa = 9.79
Molecular weight: 4.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.507
IPC2_protein 11.184
IPC_protein 12.749
Toseland 12.925
ProMoST 13.408
Dawson 12.925
Bjellqvist 12.91
Wikipedia 13.393
Rodwell 12.735
Grimsley 12.954
Solomon 13.408
Lehninger 13.305
Nozaki 12.91
DTASelect 12.91
Thurlkill 12.91
EMBOSS 13.408
Sillero 12.91
Patrickios 12.457
IPC_peptide 13.408
IPC2_peptide 12.398
IPC2.peptide.svr19 9.111
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4049
0
4049
1328520
29
4725
328.1
35.09
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.842 ± 0.046
0.681 ± 0.011
6.22 ± 0.034
5.88 ± 0.034
2.597 ± 0.023
9.397 ± 0.038
2.196 ± 0.021
2.749 ± 0.026
1.304 ± 0.023
10.766 ± 0.058
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.7 ± 0.017
1.522 ± 0.02
6.093 ± 0.031
3.027 ± 0.022
8.059 ± 0.05
5.409 ± 0.029
6.299 ± 0.054
9.828 ± 0.039
1.63 ± 0.017
1.802 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here