Bacillus oleivorans

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4443 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A285CH50|A0A285CH50_9BACI Cardiolipin synthase OS=Bacillus oleivorans OX=1448271 GN=SAMN05877753_101240 PE=4 SV=1
MM1 pKa = 7.47KK2 pKa = 10.44KK3 pKa = 9.65NWLIKK8 pKa = 10.46GFALLLIMMLLVAGCSQDD26 pKa = 3.71ADD28 pKa = 3.53EE29 pKa = 5.36GQGNTNEE36 pKa = 4.25PDD38 pKa = 3.35SGEE41 pKa = 4.27TTDD44 pKa = 5.17DD45 pKa = 3.38QDD47 pKa = 3.76NGTTEE52 pKa = 4.22EE53 pKa = 4.17PAEE56 pKa = 4.09PLTVKK61 pKa = 10.39FAAQNDD67 pKa = 3.99NTPATQAAIDD77 pKa = 4.36AFNASQDD84 pKa = 3.54QYY86 pKa = 9.09VVEE89 pKa = 4.25WVEE92 pKa = 3.95MTNDD96 pKa = 3.23SGQMHH101 pKa = 7.06DD102 pKa = 3.72QLLNSLSSGSSEE114 pKa = 4.1YY115 pKa = 10.88DD116 pKa = 3.41VLSLDD121 pKa = 3.99VVWAGEE127 pKa = 4.02FAGAGYY133 pKa = 10.54LEE135 pKa = 5.27PIDD138 pKa = 4.42VKK140 pKa = 10.35MDD142 pKa = 3.4EE143 pKa = 4.69AGYY146 pKa = 10.74SIDD149 pKa = 4.55DD150 pKa = 3.97FNAGSMTSGNYY161 pKa = 9.87KK162 pKa = 10.31GIQYY166 pKa = 7.42TLPFFPDD173 pKa = 3.49LGLLYY178 pKa = 10.17YY179 pKa = 10.6RR180 pKa = 11.84SDD182 pKa = 3.06IVSEE186 pKa = 3.72EE187 pKa = 4.04DD188 pKa = 3.26AAKK191 pKa = 10.65LEE193 pKa = 4.44SGEE196 pKa = 4.05YY197 pKa = 9.46TYY199 pKa = 11.54EE200 pKa = 3.99EE201 pKa = 4.65LGQMAEE207 pKa = 4.12KK208 pKa = 10.71YY209 pKa = 8.63MNEE212 pKa = 4.0SGTSYY217 pKa = 11.33GFVFQSAQYY226 pKa = 10.7EE227 pKa = 4.5GLTVNATEE235 pKa = 4.38FTSEE239 pKa = 4.23YY240 pKa = 10.12KK241 pKa = 10.61DD242 pKa = 3.54LSAGLEE248 pKa = 4.07TMYY251 pKa = 11.28SFVTSSFVPTDD262 pKa = 3.01ILNFQEE268 pKa = 5.85GEE270 pKa = 4.04THH272 pKa = 6.9TNFEE276 pKa = 3.69QGNAVFARR284 pKa = 11.84NWPYY288 pKa = 10.61QYY290 pKa = 11.4GRR292 pKa = 11.84IKK294 pKa = 10.48GQEE297 pKa = 4.18DD298 pKa = 4.84GVTVTPEE305 pKa = 3.82QVGIAPLPNGGSVGGWLLSINKK327 pKa = 9.79SSEE330 pKa = 4.13NKK332 pKa = 9.88DD333 pKa = 3.56GAWEE337 pKa = 4.63FIKK340 pKa = 10.75FLAGPEE346 pKa = 4.26GQKK349 pKa = 10.38IMSTGGGYY357 pKa = 10.57LPGYY361 pKa = 9.5NALLEE366 pKa = 4.51DD367 pKa = 4.31EE368 pKa = 4.96EE369 pKa = 5.04VVASNEE375 pKa = 3.99LLSNPGFQNALATTIARR392 pKa = 11.84PVSPDD397 pKa = 3.24YY398 pKa = 11.56AKK400 pKa = 10.96VSDD403 pKa = 4.3TIQVNVHH410 pKa = 6.54KK411 pKa = 10.01YY412 pKa = 9.19LSSGEE417 pKa = 4.09GLEE420 pKa = 4.28EE421 pKa = 3.67AVQAIEE427 pKa = 3.89AALAAEE433 pKa = 4.35

Molecular weight: 46.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A285CGU1|A0A285CGU1_9BACI Uncharacterized protein OS=Bacillus oleivorans OX=1448271 GN=SAMN05877753_101129 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.34QPNKK9 pKa = 8.21RR10 pKa = 11.84KK11 pKa = 9.62RR12 pKa = 11.84SKK14 pKa = 10.24VHH16 pKa = 6.26GFRR19 pKa = 11.84QRR21 pKa = 11.84MSTANGRR28 pKa = 11.84KK29 pKa = 8.48VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.94VLSAA44 pKa = 4.11

Molecular weight: 5.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4443

0

4443

1266437

29

2147

285.0

32.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.968 ± 0.041

0.685 ± 0.012

4.981 ± 0.029

7.616 ± 0.037

4.662 ± 0.034

6.962 ± 0.035

2.077 ± 0.017

8.025 ± 0.037

6.629 ± 0.038

9.876 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.617 ± 0.018

4.327 ± 0.024

3.815 ± 0.023

3.829 ± 0.022

4.017 ± 0.028

5.969 ± 0.029

5.337 ± 0.025

6.971 ± 0.03

1.094 ± 0.016

3.543 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski