Amino acid dipepetide frequency for Bacillus oleivorans

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.013AlaAla: 6.013 ± 0.084	0.591AlaCys: 0.591 ± 0.023	3.321AlaAsp: 3.321 ± 0.057	4.934AlaGlu: 4.934 ± 0.076	3.246AlaPhe: 3.246 ± 0.059	5.409AlaGly: 5.409 ± 0.073	1.265AlaHis: 1.265 ± 0.033	6.071AlaIle: 6.071 ± 0.081	4.597AlaLys: 4.597 ± 0.066	7.133AlaLeu: 7.133 ± 0.085	1.878AlaMet: 1.878 ± 0.043	2.78AlaAsn: 2.78 ± 0.049	2.109AlaPro: 2.109 ± 0.048	2.25AlaGln: 2.25 ± 0.05	2.611AlaArg: 2.611 ± 0.048	3.93AlaSer: 3.93 ± 0.057	3.269AlaThr: 3.269 ± 0.058	5.359AlaVal: 5.359 ± 0.075	0.651AlaTrp: 0.651 ± 0.026	2.265AlaTyr: 2.265 ± 0.038	0.0AlaXaa: 0.0 ± 0.0
Cys	0.378CysAla: 0.378 ± 0.018	0.088CysCys: 0.088 ± 0.01	0.35CysAsp: 0.35 ± 0.016	0.44CysGlu: 0.44 ± 0.02	0.32CysPhe: 0.32 ± 0.016	0.63CysGly: 0.63 ± 0.026	0.198CysHis: 0.198 ± 0.015	0.533CysIle: 0.533 ± 0.019	0.388CysLys: 0.388 ± 0.018	0.666CysLeu: 0.666 ± 0.026	0.16CysMet: 0.16 ± 0.012	0.283CysAsn: 0.283 ± 0.017	0.339CysPro: 0.339 ± 0.017	0.203CysGln: 0.203 ± 0.013	0.305CysArg: 0.305 ± 0.022	0.482CysSer: 0.482 ± 0.02	0.371CysThr: 0.371 ± 0.017	0.407CysVal: 0.407 ± 0.018	0.073CysTrp: 0.073 ± 0.007	0.241CysTyr: 0.241 ± 0.014	0.0CysXaa: 0.0 ± 0.0
Asp	3.138AspAla: 3.138 ± 0.059	0.35AspCys: 0.35 ± 0.02	2.251AspAsp: 2.251 ± 0.051	4.27AspGlu: 4.27 ± 0.067	2.509AspPhe: 2.509 ± 0.048	3.451AspGly: 3.451 ± 0.059	1.179AspHis: 1.179 ± 0.031	3.997AspIle: 3.997 ± 0.061	2.561AspLys: 2.561 ± 0.049	5.061AspLeu: 5.061 ± 0.061	1.195AspMet: 1.195 ± 0.034	1.59AspAsn: 1.59 ± 0.037	2.351AspPro: 2.351 ± 0.045	2.209AspGln: 2.209 ± 0.041	2.295AspArg: 2.295 ± 0.039	2.588AspSer: 2.588 ± 0.045	2.421AspThr: 2.421 ± 0.048	3.624AspVal: 3.624 ± 0.066	0.675AspTrp: 0.675 ± 0.023	2.1AspTyr: 2.1 ± 0.042	0.0AspXaa: 0.0 ± 0.0
Glu	5.464GluAla: 5.464 ± 0.081	0.346GluCys: 0.346 ± 0.019	3.806GluAsp: 3.806 ± 0.07	7.105GluGlu: 7.105 ± 0.115	2.653GluPhe: 2.653 ± 0.049	4.463GluGly: 4.463 ± 0.064	1.483GluHis: 1.483 ± 0.036	6.018GluIle: 6.018 ± 0.073	6.541GluLys: 6.541 ± 0.087	7.169GluLeu: 7.169 ± 0.08	2.196GluMet: 2.196 ± 0.042	3.849GluAsn: 3.849 ± 0.054	2.251GluPro: 2.251 ± 0.04	3.25GluGln: 3.25 ± 0.055	3.387GluArg: 3.387 ± 0.071	3.664GluSer: 3.664 ± 0.055	3.954GluThr: 3.954 ± 0.06	5.276GluVal: 5.276 ± 0.074	0.915GluTrp: 0.915 ± 0.023	2.367GluTyr: 2.367 ± 0.046	0.0GluXaa: 0.0 ± 0.0
Phe	3.068PheAla: 3.068 ± 0.062	0.33PheCys: 0.33 ± 0.016	2.315PheAsp: 2.315 ± 0.042	2.911PheGlu: 2.911 ± 0.048	2.393PhePhe: 2.393 ± 0.062	3.393PheGly: 3.393 ± 0.065	1.066PheHis: 1.066 ± 0.032	4.125PheIle: 4.125 ± 0.074	2.335PheLys: 2.335 ± 0.045	4.821PheLeu: 4.821 ± 0.076	1.143PheMet: 1.143 ± 0.032	1.93PheAsn: 1.93 ± 0.037	1.889PhePro: 1.889 ± 0.045	1.83PheGln: 1.83 ± 0.045	1.646PheArg: 1.646 ± 0.036	3.298PheSer: 3.298 ± 0.053	2.753PheThr: 2.753 ± 0.051	3.077PheVal: 3.077 ± 0.052	0.532PheTrp: 0.532 ± 0.027	1.763PheTyr: 1.763 ± 0.042	0.0PheXaa: 0.0 ± 0.0
Gly	4.835GlyAla: 4.835 ± 0.07	0.598GlyCys: 0.598 ± 0.022	3.172GlyAsp: 3.172 ± 0.05	4.698GlyGlu: 4.698 ± 0.067	3.518GlyPhe: 3.518 ± 0.06	4.844GlyGly: 4.844 ± 0.103	1.439GlyHis: 1.439 ± 0.037	6.178GlyIle: 6.178 ± 0.089	4.825GlyLys: 4.825 ± 0.062	6.737GlyLeu: 6.737 ± 0.085	2.041GlyMet: 2.041 ± 0.047	2.818GlyAsn: 2.818 ± 0.061	1.91GlyPro: 1.91 ± 0.056	2.269GlyGln: 2.269 ± 0.044	2.676GlyArg: 2.676 ± 0.047	3.992GlySer: 3.992 ± 0.072	4.262GlyThr: 4.262 ± 0.058	5.187GlyVal: 5.187 ± 0.075	0.858GlyTrp: 0.858 ± 0.027	2.768GlyTyr: 2.768 ± 0.049	0.0GlyXaa: 0.0 ± 0.0
His	1.31HisAla: 1.31 ± 0.037	0.197HisCys: 0.197 ± 0.013	1.027HisAsp: 1.027 ± 0.028	1.395HisGlu: 1.395 ± 0.034	1.092HisPhe: 1.092 ± 0.027	1.347HisGly: 1.347 ± 0.037	0.656HisHis: 0.656 ± 0.026	1.588HisIle: 1.588 ± 0.04	1.047HisLys: 1.047 ± 0.035	2.142HisLeu: 2.142 ± 0.048	0.475HisMet: 0.475 ± 0.02	0.764HisAsn: 0.764 ± 0.022	1.176HisPro: 1.176 ± 0.035	0.854HisGln: 0.854 ± 0.027	0.88HisArg: 0.88 ± 0.03	1.229HisSer: 1.229 ± 0.029	1.047HisThr: 1.047 ± 0.026	1.421HisVal: 1.421 ± 0.035	0.24HisTrp: 0.24 ± 0.014	0.881HisTyr: 0.881 ± 0.027	0.0HisXaa: 0.0 ± 0.0
Ile	5.887IleAla: 5.887 ± 0.064	0.637IleCys: 0.637 ± 0.024	4.236IleAsp: 4.236 ± 0.058	6.108IleGlu: 6.108 ± 0.073	3.639IlePhe: 3.639 ± 0.071	6.044IleGly: 6.044 ± 0.082	1.792IleHis: 1.792 ± 0.042	5.975IleIle: 5.975 ± 0.076	4.652IleLys: 4.652 ± 0.065	7.666IleLeu: 7.666 ± 0.1	1.856IleMet: 1.856 ± 0.038	3.263IleAsn: 3.263 ± 0.054	3.551IlePro: 3.551 ± 0.06	3.28IleGln: 3.28 ± 0.056	3.314IleArg: 3.314 ± 0.049	5.126IleSer: 5.126 ± 0.067	4.334IleThr: 4.334 ± 0.06	5.59IleVal: 5.59 ± 0.077	0.724IleTrp: 0.724 ± 0.022	2.573IleTyr: 2.573 ± 0.049	0.0IleXaa: 0.0 ± 0.0
Lys	4.621LysAla: 4.621 ± 0.068	0.294LysCys: 0.294 ± 0.016	3.506LysAsp: 3.506 ± 0.053	6.518LysGlu: 6.518 ± 0.079	1.903LysPhe: 1.903 ± 0.042	4.453LysGly: 4.453 ± 0.055	1.285LysHis: 1.285 ± 0.033	4.705LysIle: 4.705 ± 0.068	5.498LysLys: 5.498 ± 0.084	5.763LysLeu: 5.763 ± 0.075	1.987LysMet: 1.987 ± 0.039	3.156LysAsn: 3.156 ± 0.05	2.285LysPro: 2.285 ± 0.046	2.957LysGln: 2.957 ± 0.056	3.231LysArg: 3.231 ± 0.055	3.342LysSer: 3.342 ± 0.052	3.399LysThr: 3.399 ± 0.05	4.498LysVal: 4.498 ± 0.064	0.884LysTrp: 0.884 ± 0.027	2.001LysTyr: 2.001 ± 0.041	0.0LysXaa: 0.0 ± 0.0
Leu	7.332LeuAla: 7.332 ± 0.076	0.677LeuCys: 0.677 ± 0.021	4.768LeuAsp: 4.768 ± 0.058	7.111LeuGlu: 7.111 ± 0.092	5.007LeuPhe: 5.007 ± 0.08	6.722LeuGly: 6.722 ± 0.093	1.97LeuHis: 1.97 ± 0.041	7.297LeuIle: 7.297 ± 0.103	6.586LeuLys: 6.586 ± 0.073	10.44LeuLeu: 10.44 ± 0.136	2.394LeuMet: 2.394 ± 0.047	4.233LeuAsn: 4.233 ± 0.059	4.03LeuPro: 4.03 ± 0.056	3.752LeuGln: 3.752 ± 0.05	3.591LeuArg: 3.591 ± 0.067	6.741LeuSer: 6.741 ± 0.079	5.615LeuThr: 5.615 ± 0.066	6.302LeuVal: 6.302 ± 0.078	0.863LeuTrp: 0.863 ± 0.025	3.326LeuTyr: 3.326 ± 0.066	0.0LeuXaa: 0.0 ± 0.0
Met	2.011MetAla: 2.011 ± 0.045	0.142MetCys: 0.142 ± 0.022	1.38MetAsp: 1.38 ± 0.03	1.986MetGlu: 1.986 ± 0.041	0.995MetPhe: 0.995 ± 0.027	1.782MetGly: 1.782 ± 0.05	0.352MetHis: 0.352 ± 0.019	2.129MetIle: 2.129 ± 0.041	2.328MetLys: 2.328 ± 0.04	2.349MetLeu: 2.349 ± 0.04	0.766MetMet: 0.766 ± 0.025	1.394MetAsn: 1.394 ± 0.03	0.89MetPro: 0.89 ± 0.027	0.8MetGln: 0.8 ± 0.026	1.049MetArg: 1.049 ± 0.028	1.586MetSer: 1.586 ± 0.034	1.548MetThr: 1.548 ± 0.036	1.746MetVal: 1.746 ± 0.036	0.204MetTrp: 0.204 ± 0.014	0.734MetTyr: 0.734 ± 0.025	0.0MetXaa: 0.0 ± 0.0
Asn	2.684AsnAla: 2.684 ± 0.05	0.275AsnCys: 0.275 ± 0.018	2.119AsnAsp: 2.119 ± 0.041	3.559AsnGlu: 3.559 ± 0.056	1.605AsnPhe: 1.605 ± 0.034	3.485AsnGly: 3.485 ± 0.054	1.04AsnHis: 1.04 ± 0.029	3.148AsnIle: 3.148 ± 0.053	2.658AsnLys: 2.658 ± 0.048	3.922AsnLeu: 3.922 ± 0.061	1.078AsnMet: 1.078 ± 0.029	1.791AsnAsn: 1.791 ± 0.049	2.395AsnPro: 2.395 ± 0.047	2.237AsnGln: 2.237 ± 0.051	2.056AsnArg: 2.056 ± 0.041	2.272AsnSer: 2.272 ± 0.051	2.071AsnThr: 2.071 ± 0.04	2.899AsnVal: 2.899 ± 0.05	0.524AsnTrp: 0.524 ± 0.019	1.457AsnTyr: 1.457 ± 0.04	0.0AsnXaa: 0.0 ± 0.0
Pro	2.421ProAla: 2.421 ± 0.042	0.204ProCys: 0.204 ± 0.013	2.269ProAsp: 2.269 ± 0.048	3.222ProGlu: 3.222 ± 0.056	2.234ProPhe: 2.234 ± 0.044	2.513ProGly: 2.513 ± 0.049	0.849ProHis: 0.849 ± 0.03	3.093ProIle: 3.093 ± 0.052	2.201ProLys: 2.201 ± 0.044	3.684ProLeu: 3.684 ± 0.059	0.842ProMet: 0.842 ± 0.035	1.882ProAsn: 1.882 ± 0.039	1.158ProPro: 1.158 ± 0.034	1.143ProGln: 1.143 ± 0.041	1.178ProArg: 1.178 ± 0.032	2.405ProSer: 2.405 ± 0.042	1.98ProThr: 1.98 ± 0.045	2.885ProVal: 2.885 ± 0.05	0.388ProTrp: 0.388 ± 0.019	1.603ProTyr: 1.603 ± 0.039	0.0ProXaa: 0.0 ± 0.0
Gln	2.83GlnAla: 2.83 ± 0.05	0.199GlnCys: 0.199 ± 0.014	1.759GlnAsp: 1.759 ± 0.041	2.926GlnGlu: 2.926 ± 0.052	1.745GlnPhe: 1.745 ± 0.041	2.215GlnGly: 2.215 ± 0.044	0.711GlnHis: 0.711 ± 0.026	2.941GlnIle: 2.941 ± 0.049	2.82GlnLys: 2.82 ± 0.053	3.977GlnLeu: 3.977 ± 0.063	1.109GlnMet: 1.109 ± 0.028	1.754GlnAsn: 1.754 ± 0.039	1.278GlnPro: 1.278 ± 0.037	1.652GlnGln: 1.652 ± 0.044	1.437GlnArg: 1.437 ± 0.034	2.239GlnSer: 2.239 ± 0.044	2.157GlnThr: 2.157 ± 0.044	2.54GlnVal: 2.54 ± 0.047	0.446GlnTrp: 0.446 ± 0.02	1.552GlnTyr: 1.552 ± 0.037	0.0GlnXaa: 0.0 ± 0.0
Arg	2.457ArgAla: 2.457 ± 0.05	0.269ArgCys: 0.269 ± 0.016	2.081ArgAsp: 2.081 ± 0.046	3.271ArgGlu: 3.271 ± 0.063	1.965ArgPhe: 1.965 ± 0.038	2.426ArgGly: 2.426 ± 0.046	0.764ArgHis: 0.764 ± 0.025	3.261ArgIle: 3.261 ± 0.055	3.08ArgLys: 3.08 ± 0.051	4.022ArgLeu: 4.022 ± 0.056	1.173ArgMet: 1.173 ± 0.032	2.001ArgAsn: 2.001 ± 0.042	1.355ArgPro: 1.355 ± 0.033	1.499ArgGln: 1.499 ± 0.035	1.721ArgArg: 1.721 ± 0.044	2.224ArgSer: 2.224 ± 0.042	2.013ArgThr: 2.013 ± 0.04	2.655ArgVal: 2.655 ± 0.056	0.437ArgTrp: 0.437 ± 0.018	1.492ArgTyr: 1.492 ± 0.037	0.0ArgXaa: 0.0 ± 0.0
Ser	3.682SerAla: 3.682 ± 0.058	0.41SerCys: 0.41 ± 0.018	2.85SerAsp: 2.85 ± 0.054	3.92SerGlu: 3.92 ± 0.059	3.47SerPhe: 3.47 ± 0.061	4.397SerGly: 4.397 ± 0.066	1.256SerHis: 1.256 ± 0.032	5.324SerIle: 5.324 ± 0.068	3.602SerLys: 3.602 ± 0.063	6.373SerLeu: 6.373 ± 0.081	1.626SerMet: 1.626 ± 0.034	2.401SerAsn: 2.401 ± 0.048	2.256SerPro: 2.256 ± 0.044	2.104SerGln: 2.104 ± 0.041	2.176SerArg: 2.176 ± 0.041	3.889SerSer: 3.889 ± 0.074	2.912SerThr: 2.912 ± 0.049	4.123SerVal: 4.123 ± 0.056	0.675SerTrp: 0.675 ± 0.028	2.243SerTyr: 2.243 ± 0.045	0.0SerXaa: 0.0 ± 0.0
Thr	3.994ThrAla: 3.994 ± 0.06	0.343ThrCys: 0.343 ± 0.018	2.711ThrAsp: 2.711 ± 0.05	3.58ThrGlu: 3.58 ± 0.06	2.67ThrPhe: 2.67 ± 0.051	4.189ThrGly: 4.189 ± 0.055	1.023ThrHis: 1.023 ± 0.029	4.633ThrIle: 4.633 ± 0.06	3.128ThrLys: 3.128 ± 0.055	5.267ThrLeu: 5.267 ± 0.066	1.199ThrMet: 1.199 ± 0.032	2.377ThrAsn: 2.377 ± 0.044	2.313ThrPro: 2.313 ± 0.048	1.451ThrGln: 1.451 ± 0.037	1.825ThrArg: 1.825 ± 0.037	3.236ThrSer: 3.236 ± 0.05	2.722ThrThr: 2.722 ± 0.054	4.231ThrVal: 4.231 ± 0.066	0.57ThrTrp: 0.57 ± 0.021	1.91ThrTyr: 1.91 ± 0.04	0.0ThrXaa: 0.0 ± 0.0
Val	4.818ValAla: 4.818 ± 0.075	0.532ValCys: 0.532 ± 0.021	3.423ValAsp: 3.423 ± 0.051	4.909ValGlu: 4.909 ± 0.069	3.215ValPhe: 3.215 ± 0.056	4.555ValGly: 4.555 ± 0.073	1.386ValHis: 1.386 ± 0.033	5.789ValIle: 5.789 ± 0.071	4.535ValLys: 4.535 ± 0.069	6.968ValLeu: 6.968 ± 0.078	1.846ValMet: 1.846 ± 0.039	2.982ValAsn: 2.982 ± 0.05	2.824ValPro: 2.824 ± 0.057	2.54ValGln: 2.54 ± 0.042	2.74ValArg: 2.74 ± 0.051	4.625ValSer: 4.625 ± 0.067	4.166ValThr: 4.166 ± 0.071	4.854ValVal: 4.854 ± 0.074	0.662ValTrp: 0.662 ± 0.023	2.344ValTyr: 2.344 ± 0.051	0.0ValXaa: 0.0 ± 0.0
Trp	0.697TrpAla: 0.697 ± 0.031	0.073TrpCys: 0.073 ± 0.008	0.554TrpAsp: 0.554 ± 0.02	0.76TrpGlu: 0.76 ± 0.022	0.56TrpPhe: 0.56 ± 0.021	0.748TrpGly: 0.748 ± 0.024	0.2TrpHis: 0.2 ± 0.014	0.94TrpIle: 0.94 ± 0.029	0.77TrpLys: 0.77 ± 0.025	1.177TrpLeu: 1.177 ± 0.029	0.34TrpMet: 0.34 ± 0.017	0.569TrpAsn: 0.569 ± 0.023	0.301TrpPro: 0.301 ± 0.019	0.366TrpGln: 0.366 ± 0.018	0.411TrpArg: 0.411 ± 0.016	0.614TrpSer: 0.614 ± 0.023	0.569TrpThr: 0.569 ± 0.021	0.775TrpVal: 0.775 ± 0.029	0.153TrpTrp: 0.153 ± 0.011	0.358TrpTyr: 0.358 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.043TyrAla: 2.043 ± 0.044	0.299TyrCys: 0.299 ± 0.017	1.918TyrAsp: 1.918 ± 0.043	2.535TyrGlu: 2.535 ± 0.044	1.881TyrPhe: 1.881 ± 0.047	2.56TyrGly: 2.56 ± 0.045	0.854TyrHis: 0.854 ± 0.028	2.503TyrIle: 2.503 ± 0.046	2.134TyrLys: 2.134 ± 0.044	3.42TyrLeu: 3.42 ± 0.051	0.867TyrMet: 0.867 ± 0.027	1.458TyrAsn: 1.458 ± 0.037	1.489TyrPro: 1.489 ± 0.034	1.643TyrGln: 1.643 ± 0.039	1.635TyrArg: 1.635 ± 0.039	2.209TyrSer: 2.209 ± 0.048	1.808TyrThr: 1.808 ± 0.041	2.262TyrVal: 2.262 ± 0.047	0.46TyrTrp: 0.46 ± 0.021	1.45TyrTyr: 1.45 ± 0.041	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4443 proteins (1266437 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---