Streptococcus phage Javan52

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 69 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6B9M7|A0A4D6B9M7_9CAUD Lysozyme OS=Streptococcus phage Javan52 OX=2548232 GN=Javan52_0005 PE=3 SV=1
MM1 pKa = 7.42LVYY4 pKa = 9.63PAIFTQDD11 pKa = 2.26SDD13 pKa = 4.73YY14 pKa = 11.74IMVTFPDD21 pKa = 3.7VPEE24 pKa = 5.63AITQGEE30 pKa = 4.43NFQEE34 pKa = 4.48AYY36 pKa = 10.14EE37 pKa = 4.16MAVEE41 pKa = 4.15VLGFALEE48 pKa = 5.12DD49 pKa = 3.46YY50 pKa = 10.31TDD52 pKa = 3.75YY53 pKa = 11.49PEE55 pKa = 4.77ASSVSDD61 pKa = 4.52LKK63 pKa = 10.98EE64 pKa = 3.72QYY66 pKa = 9.46PDD68 pKa = 3.46SDD70 pKa = 3.42IALIGIDD77 pKa = 3.72MIAYY81 pKa = 6.12MKK83 pKa = 10.17KK84 pKa = 8.24YY85 pKa = 9.68HH86 pKa = 5.89SKK88 pKa = 10.45KK89 pKa = 8.6VRR91 pKa = 11.84KK92 pKa = 9.32NVTIPEE98 pKa = 4.25WLNNAAEE105 pKa = 4.35DD106 pKa = 3.85KK107 pKa = 10.81NLNFSQVLTEE117 pKa = 4.09ALEE120 pKa = 4.63LKK122 pKa = 10.44LQAA125 pKa = 4.95

Molecular weight: 14.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6B8C4|A0A4D6B8C4_9CAUD Uncharacterized protein OS=Streptococcus phage Javan52 OX=2548232 GN=Javan52_0046 PE=4 SV=1
MM1 pKa = 7.32IPKK4 pKa = 9.84FRR6 pKa = 11.84VFNKK10 pKa = 9.57KK11 pKa = 9.23AKK13 pKa = 10.04KK14 pKa = 8.78MYY16 pKa = 10.54SIDD19 pKa = 3.1GYY21 pKa = 10.58IYY23 pKa = 9.96FKK25 pKa = 10.89RR26 pKa = 11.84RR27 pKa = 11.84TARR30 pKa = 11.84PLQVANYY37 pKa = 9.59RR38 pKa = 11.84ITDD41 pKa = 3.74TVKK44 pKa = 10.56QDD46 pKa = 3.1TKK48 pKa = 11.31AAA50 pKa = 3.88

Molecular weight: 5.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

69

0

69

12998

38

1361

188.4

21.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.455 ± 0.554

0.469 ± 0.083

6.516 ± 0.31

7.409 ± 0.464

4.316 ± 0.308

5.955 ± 0.493

1.385 ± 0.139

6.909 ± 0.267

9.517 ± 0.437

8.178 ± 0.256

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.4 ± 0.173

6.001 ± 0.269

2.862 ± 0.14

3.785 ± 0.213

3.985 ± 0.241

6.386 ± 0.253

5.962 ± 0.325

6.101 ± 0.226

1.046 ± 0.089

4.362 ± 0.316
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski