Ornithinimicrobium pratense
Average proteome isoelectric point is 6.09
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3076 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5J6V240|A0A5J6V240_9MICO CHAT domain-containing protein OS=Ornithinimicrobium pratense OX=2593973 GN=FY030_02570 PE=4 SV=1
MM1 pKa = 7.44 HH2 pKa = 7.64 RR3 pKa = 11.84 PFTLTALLAAAALALSACGSDD24 pKa = 3.69 SAVEE28 pKa = 4.1 NADD31 pKa = 3.6 DD32 pKa = 5.02 PGPLTVGVSPLPHH45 pKa = 7.7 AEE47 pKa = 3.47 ILQFVDD53 pKa = 5.14 DD54 pKa = 4.78 EE55 pKa = 4.43 LAEE58 pKa = 4.11 EE59 pKa = 4.64 AGLDD63 pKa = 3.65 LEE65 pKa = 5.0 LVEE68 pKa = 4.69 FTDD71 pKa = 4.0 YY72 pKa = 11.07 LQPNVALDD80 pKa = 4.62 DD81 pKa = 4.5 GSLDD85 pKa = 3.48 ANYY88 pKa = 9.4 FQHH91 pKa = 6.97 APYY94 pKa = 10.26 LAEE97 pKa = 3.9 QEE99 pKa = 4.21 AAAGYY104 pKa = 10.19 DD105 pKa = 3.87 FEE107 pKa = 6.28 AVTPVHH113 pKa = 6.77 LEE115 pKa = 3.67 PLGLYY120 pKa = 10.09 SATLGSVEE128 pKa = 5.02 EE129 pKa = 4.69 IPDD132 pKa = 3.87 GAQIAIPNDD141 pKa = 3.45 PTNGARR147 pKa = 11.84 ALHH150 pKa = 6.44 LLAEE154 pKa = 4.32 QGLLTLADD162 pKa = 3.69 TDD164 pKa = 4.01 AASATVLDD172 pKa = 4.3 IEE174 pKa = 5.38 DD175 pKa = 3.81 NARR178 pKa = 11.84 DD179 pKa = 3.64 LQFVEE184 pKa = 5.27 IEE186 pKa = 4.0 AAQLPRR192 pKa = 11.84 SLPDD196 pKa = 2.86 VDD198 pKa = 3.89 AAVINGNYY206 pKa = 9.89 AIQADD211 pKa = 4.86 LSPATDD217 pKa = 3.46 ALAAEE222 pKa = 4.6 APEE225 pKa = 4.52 DD226 pKa = 3.59 NPYY229 pKa = 11.67 ANLLVVRR236 pKa = 11.84 AGDD239 pKa = 3.51 EE240 pKa = 3.9 SDD242 pKa = 3.38 PRR244 pKa = 11.84 IVQLAEE250 pKa = 3.86 LLGSEE255 pKa = 4.42 EE256 pKa = 3.9 VAQFIEE262 pKa = 4.31 EE263 pKa = 4.58 TYY265 pKa = 10.95 DD266 pKa = 3.4 GAVISARR273 pKa = 11.84 SAA275 pKa = 3.04
Molecular weight: 28.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.746
IPC2_protein 3.579
IPC_protein 3.592
Toseland 3.376
ProMoST 3.745
Dawson 3.579
Bjellqvist 3.732
Wikipedia 3.49
Rodwell 3.414
Grimsley 3.287
Solomon 3.567
Lehninger 3.516
Nozaki 3.681
DTASelect 3.897
Thurlkill 3.427
EMBOSS 3.503
Sillero 3.706
Patrickios 1.1
IPC_peptide 3.554
IPC2_peptide 3.681
IPC2.peptide.svr19 3.662
Protein with the highest isoelectric point:
>tr|A0A5J6V9A1|A0A5J6V9A1_9MICO TetR/AcrR family transcriptional regulator OS=Ornithinimicrobium pratense OX=2593973 GN=FY030_14235 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.0 RR4 pKa = 11.84 TFQPNNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 AKK15 pKa = 9.97 KK16 pKa = 9.33 HH17 pKa = 4.25 GFRR20 pKa = 11.84 LRR22 pKa = 11.84 MRR24 pKa = 11.84 TRR26 pKa = 11.84 AGRR29 pKa = 11.84 AILNARR35 pKa = 11.84 RR36 pKa = 11.84 TKK38 pKa = 10.61 GRR40 pKa = 11.84 SSLSAA45 pKa = 3.62
Molecular weight: 5.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.676
Grimsley 13.086
Solomon 13.539
Lehninger 13.437
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.398
IPC_peptide 13.539
IPC2_peptide 12.53
IPC2.peptide.svr19 9.215
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3076
0
3076
1029995
24
2025
334.8
35.89
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.759 ± 0.06
0.651 ± 0.013
6.117 ± 0.04
6.07 ± 0.043
2.509 ± 0.026
9.479 ± 0.04
2.34 ± 0.021
3.35 ± 0.031
1.529 ± 0.025
10.686 ± 0.059
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.933 ± 0.017
1.55 ± 0.021
5.905 ± 0.035
3.424 ± 0.021
7.791 ± 0.045
5.066 ± 0.028
6.023 ± 0.032
9.392 ± 0.04
1.602 ± 0.018
1.823 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here