Colwellia psychrerythraea (strain 34H / ATCC BAA-681) (Vibrio psychroerythus)
Average proteome isoelectric point is 6.36
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4872 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q47U48|Q47U48_COLP3 OmpA family protein OS=Colwellia psychrerythraea (strain 34H / ATCC BAA-681) OX=167879 GN=CPS_5036 PE=4 SV=1
MM1 pKa = 7.72 KK2 pKa = 9.09 KK3 pKa = 8.33 TLLTIALLSSLSTTAFANQAGDD25 pKa = 3.19 ILVRR29 pKa = 11.84 GGVTMVSPSSNEE41 pKa = 3.58 ATVLLDD47 pKa = 4.11 GSATPLSVSVDD58 pKa = 3.78 DD59 pKa = 4.04 NTQFGLNLVYY69 pKa = 10.6 FFDD72 pKa = 4.68 SNWAIEE78 pKa = 4.34 LLAATPFTHH87 pKa = 7.67 DD88 pKa = 5.19 VILHH92 pKa = 6.09 DD93 pKa = 4.91 PKK95 pKa = 11.2 GATNDD100 pKa = 3.03 IYY102 pKa = 11.28 GIDD105 pKa = 3.9 LNGATLAEE113 pKa = 4.6 VSHH116 pKa = 6.6 LPPTLSALYY125 pKa = 10.78 YY126 pKa = 10.08 FDD128 pKa = 4.11 TGTAFKK134 pKa = 9.74 PYY136 pKa = 10.2 VGVGVNYY143 pKa = 9.81 TIFFDD148 pKa = 4.16 EE149 pKa = 5.53 DD150 pKa = 3.66 FTSTPQAAGFSNLEE164 pKa = 4.14 LDD166 pKa = 4.14 GSWGYY171 pKa = 10.71 SVQLGLDD178 pKa = 3.53 YY179 pKa = 11.17 EE180 pKa = 4.52 LDD182 pKa = 4.04 KK183 pKa = 11.39 NWSVNVSARR192 pKa = 11.84 YY193 pKa = 9.45 IDD195 pKa = 4.18 INTKK199 pKa = 9.02 ATFNIDD205 pKa = 2.86 NALGVDD211 pKa = 3.82 GGLVGKK217 pKa = 10.59 GSASVDD223 pKa = 3.62 VDD225 pKa = 3.35 PMVYY229 pKa = 10.27 SVMLGYY235 pKa = 8.95 TFF237 pKa = 5.14
Molecular weight: 25.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.728
IPC2_protein 3.91
IPC_protein 3.923
Toseland 3.694
ProMoST 4.101
Dawson 3.935
Bjellqvist 4.088
Wikipedia 3.897
Rodwell 3.745
Grimsley 3.605
Solomon 3.923
Lehninger 3.884
Nozaki 4.05
DTASelect 4.329
Thurlkill 3.757
EMBOSS 3.897
Sillero 4.05
Patrickios 1.1
IPC_peptide 3.91
IPC2_peptide 4.024
IPC2.peptide.svr19 3.93
Protein with the highest isoelectric point:
>tr|Q47U79|Q47U79_COLP3 Acyltransferase family protein OS=Colwellia psychrerythraea (strain 34H / ATCC BAA-681) OX=167879 GN=CPS_5005 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.46 RR12 pKa = 11.84 KK13 pKa = 9.37 RR14 pKa = 11.84 NHH16 pKa = 5.37 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.33 NGRR28 pKa = 11.84 AVIARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.35 GRR39 pKa = 11.84 ARR41 pKa = 11.84 LSAA44 pKa = 3.91
Molecular weight: 5.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4872
0
4872
1504478
15
3758
308.8
34.34
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.122 ± 0.036
1.011 ± 0.013
5.552 ± 0.037
5.912 ± 0.032
4.366 ± 0.026
6.369 ± 0.036
2.15 ± 0.017
7.162 ± 0.03
6.044 ± 0.033
10.325 ± 0.047
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.423 ± 0.017
5.066 ± 0.024
3.506 ± 0.021
4.514 ± 0.029
3.749 ± 0.027
7.209 ± 0.029
5.632 ± 0.027
6.614 ± 0.025
1.126 ± 0.013
3.147 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here