Winogradskyella sp. PC-19

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Winogradskyella; unclassified Winogradskyella

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2717 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y0MEY8|A0A1Y0MEY8_9FLAO Uncharacterized protein OS=Winogradskyella sp. PC-19 OX=754417 GN=BTO05_02440 PE=4 SV=1
MM1 pKa = 7.86RR2 pKa = 11.84LYY4 pKa = 11.07LSIIFFLTCSSISFSQLLFDD24 pKa = 4.93DD25 pKa = 4.18EE26 pKa = 4.37AVIRR30 pKa = 11.84GLTGITSYY38 pKa = 11.3DD39 pKa = 3.43GNGNGLSFADD49 pKa = 4.13YY50 pKa = 11.4NNDD53 pKa = 3.29GLDD56 pKa = 4.0DD57 pKa = 3.85VTLPTGNDD65 pKa = 3.16EE66 pKa = 4.13TLKK69 pKa = 10.67FYY71 pKa = 11.42KK72 pKa = 10.21NVNGVFVEE80 pKa = 4.27DD81 pKa = 3.88RR82 pKa = 11.84VLTPAIDD89 pKa = 3.5YY90 pKa = 7.57KK91 pKa = 10.34TRR93 pKa = 11.84SVSWVDD99 pKa = 3.19YY100 pKa = 11.39DD101 pKa = 4.15NDD103 pKa = 3.63GDD105 pKa = 3.88RR106 pKa = 11.84DD107 pKa = 4.0LFVVSDD113 pKa = 3.76IEE115 pKa = 4.25GSRR118 pKa = 11.84LFRR121 pKa = 11.84QDD123 pKa = 3.52PEE125 pKa = 4.33LLFVDD130 pKa = 3.99VTVSSGLFTDD140 pKa = 6.06AIDD143 pKa = 3.89TYY145 pKa = 10.75SVSWGDD151 pKa = 3.66INNDD155 pKa = 2.76GCLDD159 pKa = 3.98LFYY162 pKa = 11.32SNRR165 pKa = 11.84TLNSLITNYY174 pKa = 10.17LFQSNCDD181 pKa = 3.24GTFTNITSTAGLSQDD196 pKa = 3.06PRR198 pKa = 11.84LTFGASFFDD207 pKa = 3.98YY208 pKa = 11.23NNDD211 pKa = 3.26GFQDD215 pKa = 3.38IYY217 pKa = 11.4VINDD221 pKa = 3.27KK222 pKa = 11.1NGSNFLYY229 pKa = 10.77EE230 pKa = 4.14NDD232 pKa = 3.75GDD234 pKa = 4.26STFTDD239 pKa = 3.75VSSQTSTGIVVDD251 pKa = 4.24AMSVTIDD258 pKa = 4.55DD259 pKa = 4.22YY260 pKa = 11.97NSDD263 pKa = 4.17GFFDD267 pKa = 4.0IYY269 pKa = 9.99ITNTPSDD276 pKa = 3.75VATPTLGSVLLKK288 pKa = 10.61NVNGQYY294 pKa = 10.49FEE296 pKa = 5.25DD297 pKa = 4.1VSSSSGTLLDD307 pKa = 3.97GWCWGSNFFDD317 pKa = 5.1ADD319 pKa = 3.21NDD321 pKa = 3.69MDD323 pKa = 4.91LDD325 pKa = 4.67LYY327 pKa = 10.71VSCIYY332 pKa = 9.42TLPGEE337 pKa = 4.18RR338 pKa = 11.84QSYY341 pKa = 9.59GFYY344 pKa = 10.74QNDD347 pKa = 3.53TLEE350 pKa = 4.46VFSEE354 pKa = 4.05PTNIGFANNDD364 pKa = 3.22IDD366 pKa = 3.91SHH368 pKa = 7.57GSVIGDD374 pKa = 3.6ADD376 pKa = 3.77NDD378 pKa = 4.09GKK380 pKa = 11.47VDD382 pKa = 3.31IAVVNNGGLVPNLWMNKK399 pKa = 7.41TVTTNNYY406 pKa = 8.44LTISLEE412 pKa = 4.12GTTSNKK418 pKa = 10.48DD419 pKa = 3.41GIGSKK424 pKa = 9.95IEE426 pKa = 3.63ISINGVKK433 pKa = 9.86QYY435 pKa = 10.8RR436 pKa = 11.84YY437 pKa = 9.47VINGEE442 pKa = 4.58GYY444 pKa = 10.74LSQNSFKK451 pKa = 10.94EE452 pKa = 4.05FFGLGEE458 pKa = 4.21NTVVDD463 pKa = 3.91YY464 pKa = 11.57VKK466 pKa = 10.81VYY468 pKa = 9.17WLSGTIDD475 pKa = 3.59TILNVTANQILNIVEE490 pKa = 4.15GSNVLSNPNLEE501 pKa = 3.85ISEE504 pKa = 4.91DD505 pKa = 4.05FLIHH509 pKa = 6.74PNPTSDD515 pKa = 3.24RR516 pKa = 11.84LSFKK520 pKa = 10.47SRR522 pKa = 11.84NIIEE526 pKa = 4.9TITMYY531 pKa = 10.83NVLGQEE537 pKa = 4.33VFKK540 pKa = 10.89SYY542 pKa = 11.2YY543 pKa = 10.44DD544 pKa = 3.6SLEE547 pKa = 3.68VDD549 pKa = 3.13IDD551 pKa = 3.52ISNLSSGAYY560 pKa = 8.78FVKK563 pKa = 10.1TSLSGVIKK571 pKa = 9.18TSRR574 pKa = 11.84IIKK577 pKa = 9.26QQ578 pKa = 3.04

Molecular weight: 63.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Y0MEZ2|A0A1Y0MEZ2_9FLAO MarR family transcriptional regulator OS=Winogradskyella sp. PC-19 OX=754417 GN=BTO05_04310 PE=4 SV=1
MM1 pKa = 8.0PKK3 pKa = 9.06RR4 pKa = 11.84TFQPSKK10 pKa = 9.13RR11 pKa = 11.84KK12 pKa = 9.48RR13 pKa = 11.84RR14 pKa = 11.84NKK16 pKa = 9.49HH17 pKa = 3.94GFRR20 pKa = 11.84EE21 pKa = 4.27RR22 pKa = 11.84MASANGRR29 pKa = 11.84KK30 pKa = 9.04VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 9.76GRR40 pKa = 11.84KK41 pKa = 8.18RR42 pKa = 11.84LSVSSDD48 pKa = 3.05LRR50 pKa = 11.84HH51 pKa = 6.32KK52 pKa = 10.54KK53 pKa = 10.01

Molecular weight: 6.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2717

0

2717

910443

33

2739

335.1

37.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.166 ± 0.046

0.736 ± 0.017

5.947 ± 0.042

6.445 ± 0.045

5.358 ± 0.044

6.226 ± 0.046

1.614 ± 0.021

8.16 ± 0.049

7.924 ± 0.075

9.142 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.088 ± 0.024

6.495 ± 0.06

3.235 ± 0.029

3.27 ± 0.025

3.39 ± 0.037

6.638 ± 0.041

5.906 ± 0.071

6.222 ± 0.034

0.994 ± 0.014

4.045 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski