Anaerobranca californiensis DSM 14826

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Proteinivoraceae; Anaerobranca; Anaerobranca californiensis

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2060 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1M6QE68|A0A1M6QE68_9FIRM Pimeloyl-ACP methyl ester carboxylesterase OS=Anaerobranca californiensis DSM 14826 OX=1120989 GN=SAMN02745227_01728 PE=4 SV=1
MM1 pKa = 7.24QEE3 pKa = 3.85ALFTILFPVAGFVLGAYY20 pKa = 9.78VSLDD24 pKa = 3.65IIKK27 pKa = 9.53MLVDD31 pKa = 3.65LVYY34 pKa = 10.47PEE36 pKa = 4.26QEE38 pKa = 4.21GEE40 pKa = 3.93HH41 pKa = 7.25DD42 pKa = 4.13EE43 pKa = 4.38NCC45 pKa = 4.02

Molecular weight: 5.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1M6M3S2|A0A1M6M3S2_9FIRM 30S ribosomal protein S18 OS=Anaerobranca californiensis DSM 14826 OX=1120989 GN=rpsR PE=3 SV=1
MM1 pKa = 7.36KK2 pKa = 9.37RR3 pKa = 11.84TYY5 pKa = 9.3QPKK8 pKa = 8.71VRR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 9.64SNHH16 pKa = 5.34GFLKK20 pKa = 10.61RR21 pKa = 11.84MSTKK25 pKa = 10.43AGRR28 pKa = 11.84NVIKK32 pKa = 10.49RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.18GRR39 pKa = 11.84KK40 pKa = 8.74RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2060

0

2060

613142

39

2843

297.6

33.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.766 ± 0.056

0.916 ± 0.021

4.775 ± 0.039

7.555 ± 0.063

4.473 ± 0.047

7.433 ± 0.046

1.587 ± 0.021

9.56 ± 0.057

8.414 ± 0.063

10.456 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.238 ± 0.024

5.067 ± 0.04

3.53 ± 0.031

3.088 ± 0.031

3.779 ± 0.039

5.049 ± 0.036

4.936 ± 0.033

6.912 ± 0.053

0.713 ± 0.016

3.753 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski