Amino acid dipepetide frequency for Anaerobranca californiensis DSM 14826

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.275AlaAla: 4.275 ± 0.112
0.556AlaCys: 0.556 ± 0.034
2.406AlaAsp: 2.406 ± 0.067
3.616AlaGlu: 3.616 ± 0.088
2.505AlaPhe: 2.505 ± 0.079
4.312AlaGly: 4.312 ± 0.103
1.036AlaHis: 1.036 ± 0.043
5.904AlaIle: 5.904 ± 0.112
4.85AlaLys: 4.85 ± 0.094
6.53AlaLeu: 6.53 ± 0.115
1.593AlaMet: 1.593 ± 0.052
2.409AlaAsn: 2.409 ± 0.069
1.652AlaPro: 1.652 ± 0.054
2.066AlaGln: 2.066 ± 0.058
2.093AlaArg: 2.093 ± 0.067
2.579AlaSer: 2.579 ± 0.07
2.831AlaThr: 2.831 ± 0.069
4.28AlaVal: 4.28 ± 0.105
0.347AlaTrp: 0.347 ± 0.023
1.817AlaTyr: 1.817 ± 0.058
0.0AlaXaa: 0.0 ± 0.0
Cys
0.488CysAla: 0.488 ± 0.033
0.135CysCys: 0.135 ± 0.016
0.46CysAsp: 0.46 ± 0.026
0.496CysGlu: 0.496 ± 0.024
0.298CysPhe: 0.298 ± 0.027
0.928CysGly: 0.928 ± 0.046
0.243CysHis: 0.243 ± 0.022
0.719CysIle: 0.719 ± 0.038
0.652CysLys: 0.652 ± 0.033
0.744CysLeu: 0.744 ± 0.037
0.158CysMet: 0.158 ± 0.016
0.515CysAsn: 0.515 ± 0.033
0.568CysPro: 0.568 ± 0.038
0.437CysGln: 0.437 ± 0.025
0.373CysArg: 0.373 ± 0.024
0.566CysSer: 0.566 ± 0.028
0.444CysThr: 0.444 ± 0.026
0.501CysVal: 0.501 ± 0.031
0.067CysTrp: 0.067 ± 0.01
0.37CysTyr: 0.37 ± 0.024
0.0CysXaa: 0.0 ± 0.0
Asp
1.908AspAla: 1.908 ± 0.061
0.54AspCys: 0.54 ± 0.036
2.009AspAsp: 2.009 ± 0.077
3.443AspGlu: 3.443 ± 0.078
2.603AspPhe: 2.603 ± 0.072
3.242AspGly: 3.242 ± 0.086
0.693AspHis: 0.693 ± 0.04
5.079AspIle: 5.079 ± 0.1
4.342AspLys: 4.342 ± 0.089
5.343AspLeu: 5.343 ± 0.118
1.018AspMet: 1.018 ± 0.036
2.448AspAsn: 2.448 ± 0.065
1.76AspPro: 1.76 ± 0.058
1.287AspGln: 1.287 ± 0.043
1.726AspArg: 1.726 ± 0.058
2.199AspSer: 2.199 ± 0.059
2.057AspThr: 2.057 ± 0.056
3.42AspVal: 3.42 ± 0.072
0.351AspTrp: 0.351 ± 0.022
2.288AspTyr: 2.288 ± 0.069
0.0AspXaa: 0.0 ± 0.0
Glu
3.668GluAla: 3.668 ± 0.095
0.391GluCys: 0.391 ± 0.028
3.573GluAsp: 3.573 ± 0.076
7.537GluGlu: 7.537 ± 0.154
2.812GluPhe: 2.812 ± 0.072
5.5GluGly: 5.5 ± 0.097
1.068GluHis: 1.068 ± 0.045
8.143GluIle: 8.143 ± 0.131
8.266GluLys: 8.266 ± 0.159
7.263GluLeu: 7.263 ± 0.13
1.936GluMet: 1.936 ± 0.06
4.483GluAsn: 4.483 ± 0.099
1.598GluPro: 1.598 ± 0.056
2.376GluGln: 2.376 ± 0.07
3.275GluArg: 3.275 ± 0.084
2.525GluSer: 2.525 ± 0.068
2.895GluThr: 2.895 ± 0.07
5.361GluVal: 5.361 ± 0.11
0.328GluTrp: 0.328 ± 0.022
2.548GluTyr: 2.548 ± 0.069
0.0GluXaa: 0.0 ± 0.0
Phe
2.711PheAla: 2.711 ± 0.067
0.44PheCys: 0.44 ± 0.03
2.088PheAsp: 2.088 ± 0.066
2.358PheGlu: 2.358 ± 0.064
2.282PhePhe: 2.282 ± 0.086
3.034PheGly: 3.034 ± 0.082
0.711PheHis: 0.711 ± 0.035
4.402PheIle: 4.402 ± 0.103
3.215PheLys: 3.215 ± 0.072
4.906PheLeu: 4.906 ± 0.113
0.977PheMet: 0.977 ± 0.045
2.526PheAsn: 2.526 ± 0.066
1.651PhePro: 1.651 ± 0.057
1.326PheGln: 1.326 ± 0.048
1.479PheArg: 1.479 ± 0.047
3.021PheSer: 3.021 ± 0.071
2.65PheThr: 2.65 ± 0.071
2.716PheVal: 2.716 ± 0.073
0.416PheTrp: 0.416 ± 0.029
1.822PheTyr: 1.822 ± 0.057
0.0PheXaa: 0.0 ± 0.0
Gly
4.493GlyAla: 4.493 ± 0.109
0.895GlyCys: 0.895 ± 0.037
3.599GlyAsp: 3.599 ± 0.084
5.641GlyGlu: 5.641 ± 0.098
3.52GlyPhe: 3.52 ± 0.087
5.423GlyGly: 5.423 ± 0.113
1.156GlyHis: 1.156 ± 0.05
7.736GlyIle: 7.736 ± 0.119
6.498GlyLys: 6.498 ± 0.12
7.152GlyLeu: 7.152 ± 0.132
1.757GlyMet: 1.757 ± 0.054
3.233GlyAsn: 3.233 ± 0.081
1.729GlyPro: 1.729 ± 0.055
2.243GlyGln: 2.243 ± 0.066
2.88GlyArg: 2.88 ± 0.071
3.497GlySer: 3.497 ± 0.073
3.536GlyThr: 3.536 ± 0.088
5.672GlyVal: 5.672 ± 0.117
0.532GlyTrp: 0.532 ± 0.033
3.135GlyTyr: 3.135 ± 0.076
0.0GlyXaa: 0.0 ± 0.0
His
0.674HisAla: 0.674 ± 0.033
0.3HisCys: 0.3 ± 0.021
0.634HisAsp: 0.634 ± 0.034
0.703HisGlu: 0.703 ± 0.034
0.784HisPhe: 0.784 ± 0.036
1.251HisGly: 1.251 ± 0.043
0.411HisHis: 0.411 ± 0.031
1.349HisIle: 1.349 ± 0.048
1.055HisLys: 1.055 ± 0.043
1.768HisLeu: 1.768 ± 0.062
0.269HisMet: 0.269 ± 0.023
0.832HisAsn: 0.832 ± 0.034
0.972HisPro: 0.972 ± 0.044
0.652HisGln: 0.652 ± 0.033
0.739HisArg: 0.739 ± 0.032
0.951HisSer: 0.951 ± 0.041
0.78HisThr: 0.78 ± 0.034
0.889HisVal: 0.889 ± 0.045
0.147HisTrp: 0.147 ± 0.015
0.713HisTyr: 0.713 ± 0.037
0.0HisXaa: 0.0 ± 0.0
Ile
5.979IleAla: 5.979 ± 0.11
0.802IleCys: 0.802 ± 0.037
5.056IleAsp: 5.056 ± 0.093
6.804IleGlu: 6.804 ± 0.122
4.376IlePhe: 4.376 ± 0.113
6.468IleGly: 6.468 ± 0.128
1.525IleHis: 1.525 ± 0.053
9.321IleIle: 9.321 ± 0.167
8.057IleLys: 8.057 ± 0.132
9.869IleLeu: 9.869 ± 0.162
1.964IleMet: 1.964 ± 0.057
4.687IleAsn: 4.687 ± 0.089
4.095IlePro: 4.095 ± 0.087
2.528IleGln: 2.528 ± 0.065
3.097IleArg: 3.097 ± 0.072
5.731IleSer: 5.731 ± 0.107
5.348IleThr: 5.348 ± 0.086
6.035IleVal: 6.035 ± 0.106
0.515IleTrp: 0.515 ± 0.031
3.34IleTyr: 3.34 ± 0.073
0.0IleXaa: 0.0 ± 0.0
Lys
4.271LysAla: 4.271 ± 0.095
0.613LysCys: 0.613 ± 0.036
4.619LysAsp: 4.619 ± 0.089
9.042LysGlu: 9.042 ± 0.133
2.699LysPhe: 2.699 ± 0.066
7.328LysGly: 7.328 ± 0.13
1.014LysHis: 1.014 ± 0.037
8.151LysIle: 8.151 ± 0.13
7.248LysLys: 7.248 ± 0.122
7.486LysLeu: 7.486 ± 0.128
2.016LysMet: 2.016 ± 0.054
4.593LysAsn: 4.593 ± 0.095
2.288LysPro: 2.288 ± 0.07
1.766LysGln: 1.766 ± 0.054
3.376LysArg: 3.376 ± 0.076
3.449LysSer: 3.449 ± 0.081
3.898LysThr: 3.898 ± 0.082
6.415LysVal: 6.415 ± 0.113
0.647LysTrp: 0.647 ± 0.03
3.218LysTyr: 3.218 ± 0.081
0.0LysXaa: 0.0 ± 0.0
Leu
7.563LeuAla: 7.563 ± 0.129
0.889LeuCys: 0.889 ± 0.036
5.24LeuAsp: 5.24 ± 0.095
8.288LeuGlu: 8.288 ± 0.144
4.384LeuPhe: 4.384 ± 0.11
7.732LeuGly: 7.732 ± 0.133
1.396LeuHis: 1.396 ± 0.045
8.504LeuIle: 8.504 ± 0.143
9.035LeuLys: 9.035 ± 0.123
10.826LeuLeu: 10.826 ± 0.17
2.194LeuMet: 2.194 ± 0.062
5.24LeuAsn: 5.24 ± 0.104
4.105LeuPro: 4.105 ± 0.092
3.322LeuGln: 3.322 ± 0.078
3.565LeuArg: 3.565 ± 0.074
5.772LeuSer: 5.772 ± 0.097
5.778LeuThr: 5.778 ± 0.089
6.719LeuVal: 6.719 ± 0.13
0.669LeuTrp: 0.669 ± 0.044
3.339LeuTyr: 3.339 ± 0.082
0.0LeuXaa: 0.0 ± 0.0
Met
2.029MetAla: 2.029 ± 0.061
0.134MetCys: 0.134 ± 0.014
1.215MetAsp: 1.215 ± 0.043
2.223MetGlu: 2.223 ± 0.064
0.837MetPhe: 0.837 ± 0.04
1.887MetGly: 1.887 ± 0.057
0.202MetHis: 0.202 ± 0.019
1.776MetIle: 1.776 ± 0.057
1.696MetLys: 1.696 ± 0.053
2.14MetLeu: 2.14 ± 0.06
0.568MetMet: 0.568 ± 0.032
0.935MetAsn: 0.935 ± 0.04
0.753MetPro: 0.753 ± 0.037
0.493MetGln: 0.493 ± 0.028
0.723MetArg: 0.723 ± 0.031
1.072MetSer: 1.072 ± 0.039
1.083MetThr: 1.083 ± 0.043
1.822MetVal: 1.822 ± 0.049
0.144MetTrp: 0.144 ± 0.016
0.652MetTyr: 0.652 ± 0.033
0.0MetXaa: 0.0 ± 0.0
Asn
2.084AsnAla: 2.084 ± 0.066
0.67AsnCys: 0.67 ± 0.036
1.908AsnAsp: 1.908 ± 0.059
2.688AsnGlu: 2.688 ± 0.074
2.712AsnPhe: 2.712 ± 0.079
3.024AsnGly: 3.024 ± 0.079
0.923AsnHis: 0.923 ± 0.041
5.456AsnIle: 5.456 ± 0.11
4.211AsnLys: 4.211 ± 0.1
6.271AsnLeu: 6.271 ± 0.127
1.005AsnMet: 1.005 ± 0.039
2.929AsnAsn: 2.929 ± 0.084
2.494AsnPro: 2.494 ± 0.063
1.535AsnGln: 1.535 ± 0.057
1.977AsnArg: 1.977 ± 0.055
2.725AsnSer: 2.725 ± 0.071
2.386AsnThr: 2.386 ± 0.068
2.813AsnVal: 2.813 ± 0.071
0.473AsnTrp: 0.473 ± 0.028
2.388AsnTyr: 2.388 ± 0.069
0.0AsnXaa: 0.0 ± 0.0
Pro
1.799ProAla: 1.799 ± 0.062
0.323ProCys: 0.323 ± 0.023
1.695ProAsp: 1.695 ± 0.058
2.596ProGlu: 2.596 ± 0.078
1.786ProPhe: 1.786 ± 0.057
2.459ProGly: 2.459 ± 0.078
0.835ProHis: 0.835 ± 0.036
3.063ProIle: 3.063 ± 0.071
2.794ProLys: 2.794 ± 0.086
3.887ProLeu: 3.887 ± 0.081
0.718ProMet: 0.718 ± 0.036
1.66ProAsn: 1.66 ± 0.058
1.089ProPro: 1.089 ± 0.041
1.562ProGln: 1.562 ± 0.049
1.15ProArg: 1.15 ± 0.045
1.737ProSer: 1.737 ± 0.053
1.662ProThr: 1.662 ± 0.058
2.729ProVal: 2.729 ± 0.066
0.303ProTrp: 0.303 ± 0.02
1.455ProTyr: 1.455 ± 0.048
0.0ProXaa: 0.0 ± 0.0
Gln
1.461GlnAla: 1.461 ± 0.054
0.263GlnCys: 0.263 ± 0.022
1.125GlnAsp: 1.125 ± 0.044
2.034GlnGlu: 2.034 ± 0.052
1.173GlnPhe: 1.173 ± 0.044
2.839GlnGly: 2.839 ± 0.073
0.496GlnHis: 0.496 ± 0.027
2.671GlnIle: 2.671 ± 0.064
2.636GlnLys: 2.636 ± 0.072
3.167GlnLeu: 3.167 ± 0.069
0.768GlnMet: 0.768 ± 0.039
1.528GlnAsn: 1.528 ± 0.044
0.954GlnPro: 0.954 ± 0.045
1.445GlnGln: 1.445 ± 0.061
1.965GlnArg: 1.965 ± 0.061
1.46GlnSer: 1.46 ± 0.055
1.414GlnThr: 1.414 ± 0.046
1.933GlnVal: 1.933 ± 0.056
0.339GlnTrp: 0.339 ± 0.024
1.205GlnTyr: 1.205 ± 0.049
0.0GlnXaa: 0.0 ± 0.0
Arg
2.011ArgAla: 2.011 ± 0.056
0.338ArgCys: 0.338 ± 0.025
2.079ArgAsp: 2.079 ± 0.061
3.758ArgGlu: 3.758 ± 0.086
1.566ArgPhe: 1.566 ± 0.054
2.887ArgGly: 2.887 ± 0.066
0.579ArgHis: 0.579 ± 0.029
3.327ArgIle: 3.327 ± 0.07
3.024ArgLys: 3.024 ± 0.076
3.634ArgLeu: 3.634 ± 0.083
1.005ArgMet: 1.005 ± 0.039
1.752ArgAsn: 1.752 ± 0.051
1.215ArgPro: 1.215 ± 0.045
1.194ArgGln: 1.194 ± 0.051
1.696ArgArg: 1.696 ± 0.05
1.559ArgSer: 1.559 ± 0.049
1.678ArgThr: 1.678 ± 0.055
2.758ArgVal: 2.758 ± 0.069
0.3ArgTrp: 0.3 ± 0.024
1.427ArgTyr: 1.427 ± 0.055
0.0ArgXaa: 0.0 ± 0.0
Ser
2.621SerAla: 2.621 ± 0.072
0.416SerCys: 0.416 ± 0.028
1.915SerAsp: 1.915 ± 0.06
2.991SerGlu: 2.991 ± 0.078
2.81SerPhe: 2.81 ± 0.072
3.751SerGly: 3.751 ± 0.076
0.895SerHis: 0.895 ± 0.044
4.764SerIle: 4.764 ± 0.087
4.055SerLys: 4.055 ± 0.071
6.124SerLeu: 6.124 ± 0.111
1.138SerMet: 1.138 ± 0.044
2.383SerAsn: 2.383 ± 0.061
2.013SerPro: 2.013 ± 0.065
1.709SerGln: 1.709 ± 0.057
1.982SerArg: 1.982 ± 0.06
2.91SerSer: 2.91 ± 0.077
2.554SerThr: 2.554 ± 0.057
3.219SerVal: 3.219 ± 0.076
0.409SerTrp: 0.409 ± 0.027
1.835SerTyr: 1.835 ± 0.054
0.0SerXaa: 0.0 ± 0.0
Thr
3.154ThrAla: 3.154 ± 0.078
0.377ThrCys: 0.377 ± 0.025
2.207ThrAsp: 2.207 ± 0.058
3.151ThrGlu: 3.151 ± 0.069
2.376ThrPhe: 2.376 ± 0.065
4.152ThrGly: 4.152 ± 0.09
0.789ThrHis: 0.789 ± 0.037
4.782ThrIle: 4.782 ± 0.099
3.467ThrLys: 3.467 ± 0.076
5.457ThrLeu: 5.457 ± 0.096
1.032ThrMet: 1.032 ± 0.035
2.313ThrAsn: 2.313 ± 0.057
2.225ThrPro: 2.225 ± 0.062
1.199ThrGln: 1.199 ± 0.044
1.52ThrArg: 1.52 ± 0.053
2.579ThrSer: 2.579 ± 0.074
2.813ThrThr: 2.813 ± 0.072
3.795ThrVal: 3.795 ± 0.077
0.313ThrTrp: 0.313 ± 0.025
1.66ThrTyr: 1.66 ± 0.049
0.0ThrXaa: 0.0 ± 0.0
Val
4.529ValAla: 4.529 ± 0.109
0.559ValCys: 0.559 ± 0.032
3.834ValAsp: 3.834 ± 0.086
5.513ValGlu: 5.513 ± 0.093
2.967ValPhe: 2.967 ± 0.073
4.842ValGly: 4.842 ± 0.113
0.938ValHis: 0.938 ± 0.038
6.633ValIle: 6.633 ± 0.118
5.721ValLys: 5.721 ± 0.1
6.842ValLeu: 6.842 ± 0.114
1.438ValMet: 1.438 ± 0.05
3.622ValAsn: 3.622 ± 0.085
2.306ValPro: 2.306 ± 0.07
1.796ValGln: 1.796 ± 0.051
2.295ValArg: 2.295 ± 0.062
3.464ValSer: 3.464 ± 0.083
3.507ValThr: 3.507 ± 0.078
5.646ValVal: 5.646 ± 0.113
0.439ValTrp: 0.439 ± 0.027
2.228ValTyr: 2.228 ± 0.057
0.0ValXaa: 0.0 ± 0.0
Trp
0.429TrpAla: 0.429 ± 0.028
0.052TrpCys: 0.052 ± 0.009
0.38TrpAsp: 0.38 ± 0.024
0.62TrpGlu: 0.62 ± 0.032
0.325TrpPhe: 0.325 ± 0.025
0.582TrpGly: 0.582 ± 0.031
0.135TrpHis: 0.135 ± 0.015
0.543TrpIle: 0.543 ± 0.034
0.455TrpLys: 0.455 ± 0.029
0.734TrpLeu: 0.734 ± 0.039
0.196TrpMet: 0.196 ± 0.02
0.325TrpAsn: 0.325 ± 0.022
0.228TrpPro: 0.228 ± 0.018
0.329TrpGln: 0.329 ± 0.024
0.271TrpArg: 0.271 ± 0.02
0.406TrpSer: 0.406 ± 0.027
0.256TrpThr: 0.256 ± 0.021
0.462TrpVal: 0.462 ± 0.028
0.099TrpTrp: 0.099 ± 0.012
0.3TrpTyr: 0.3 ± 0.029
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.51TyrAla: 1.51 ± 0.049
0.468TyrCys: 0.468 ± 0.026
1.711TyrAsp: 1.711 ± 0.058
2.044TyrGlu: 2.044 ± 0.069
1.915TyrPhe: 1.915 ± 0.06
2.683TyrGly: 2.683 ± 0.063
0.825TyrHis: 0.825 ± 0.035
3.277TyrIle: 3.277 ± 0.075
2.862TyrLys: 2.862 ± 0.077
4.42TyrLeu: 4.42 ± 0.106
0.631TyrMet: 0.631 ± 0.03
2.259TyrAsn: 2.259 ± 0.059
1.606TyrPro: 1.606 ± 0.057
1.611TyrGln: 1.611 ± 0.053
1.605TyrArg: 1.605 ± 0.057
2.295TyrSer: 2.295 ± 0.057
1.792TyrThr: 1.792 ± 0.061
1.936TyrVal: 1.936 ± 0.06
0.289TyrTrp: 0.289 ± 0.02
1.796TyrTyr: 1.796 ± 0.058
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2060 proteins (613142 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski