Arsenicitalea aurantiaca
Average proteome isoelectric point is 6.27
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3476 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A433XF31|A0A433XF31_9RHIZ Outer membrane protein assembly factor BamA OS=Arsenicitalea aurantiaca OX=1783274 GN=bamA PE=3 SV=1
MM1 pKa = 7.8 KK2 pKa = 9.48 LTTKK6 pKa = 10.79 LLAASAASVLMTASAFAADD25 pKa = 4.86 LYY27 pKa = 11.62 VPIEE31 pKa = 4.0 QPYY34 pKa = 8.33 VPEE37 pKa = 4.08 VAAAAPLGWTGAYY50 pKa = 9.87 VGVHH54 pKa = 6.5 AGYY57 pKa = 10.61 GWGQFDD63 pKa = 5.15 LAGDD67 pKa = 4.06 DD68 pKa = 3.46 EE69 pKa = 5.16 TTFFTPGDD77 pKa = 3.75 SDD79 pKa = 3.67 VDD81 pKa = 3.24 GWLAGVQAGYY91 pKa = 10.38 NVNLDD96 pKa = 3.64 GVVLGIEE103 pKa = 4.73 GDD105 pKa = 3.88 IAWADD110 pKa = 3.79 LNGSATIGTGDD121 pKa = 3.89 DD122 pKa = 3.46 AVTVSSNIDD131 pKa = 3.25 WIGTIRR137 pKa = 11.84 GRR139 pKa = 11.84 VGFAADD145 pKa = 3.47 AFLLYY150 pKa = 9.07 GTAGVAFAGASMDD163 pKa = 3.78 VAALEE168 pKa = 4.12 PTEE171 pKa = 5.26 AFSDD175 pKa = 4.3 DD176 pKa = 3.7 NVHH179 pKa = 6.07 TGWVVGAGVEE189 pKa = 4.53 TMVTDD194 pKa = 4.86 NISLKK199 pKa = 10.85 AEE201 pKa = 4.0 YY202 pKa = 9.88 LYY204 pKa = 10.87 HH205 pKa = 7.72 DD206 pKa = 5.23 FGNEE210 pKa = 3.71 TFSFDD215 pKa = 3.96 DD216 pKa = 3.85 TNATDD221 pKa = 4.0 ASFNVQTAKK230 pKa = 10.41 IGLNFHH236 pKa = 7.0 FF237 pKa = 5.42
Molecular weight: 24.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.691
IPC2_protein 3.668
IPC_protein 3.681
Toseland 3.452
ProMoST 3.872
Dawson 3.694
Bjellqvist 3.846
Wikipedia 3.656
Rodwell 3.516
Grimsley 3.363
Solomon 3.681
Lehninger 3.643
Nozaki 3.808
DTASelect 4.088
Thurlkill 3.528
EMBOSS 3.656
Sillero 3.808
Patrickios 1.036
IPC_peptide 3.668
IPC2_peptide 3.783
IPC2.peptide.svr19 3.734
Protein with the highest isoelectric point:
>tr|A0A433XF98|A0A433XF98_9RHIZ SMP-30/gluconolactonase/LRE family protein OS=Arsenicitalea aurantiaca OX=1783274 GN=EMQ25_06245 PE=4 SV=1
MM1 pKa = 7.77 RR2 pKa = 11.84 RR3 pKa = 11.84 RR4 pKa = 11.84 AQKK7 pKa = 9.89 SPPRR11 pKa = 11.84 AAHH14 pKa = 5.98 HH15 pKa = 6.12 RR16 pKa = 11.84 RR17 pKa = 11.84 GVRR20 pKa = 11.84 PKK22 pKa = 10.18 PKK24 pKa = 9.74 RR25 pKa = 11.84 APPLPRR31 pKa = 11.84 TLPRR35 pKa = 11.84 LRR37 pKa = 11.84 RR38 pKa = 11.84 AFARR42 pKa = 11.84 WRR44 pKa = 11.84 QSGMRR49 pKa = 11.84 QRR51 pKa = 11.84 HH52 pKa = 5.03 PPWRR56 pKa = 11.84 GTPPAQRR63 pKa = 11.84 QRR65 pKa = 11.84 GG66 pKa = 3.6
Molecular weight: 7.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.5
IPC2_protein 11.491
IPC_protein 13.1
Toseland 13.261
ProMoST 13.759
Dawson 13.261
Bjellqvist 13.261
Wikipedia 13.729
Rodwell 12.822
Grimsley 13.29
Solomon 13.759
Lehninger 13.656
Nozaki 13.261
DTASelect 13.261
Thurlkill 13.261
EMBOSS 13.759
Sillero 13.261
Patrickios 12.544
IPC_peptide 13.759
IPC2_peptide 12.749
IPC2.peptide.svr19 9.373
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3476
0
3476
1110746
20
3988
319.5
34.52
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.891 ± 0.059
0.694 ± 0.012
5.515 ± 0.039
6.08 ± 0.04
3.769 ± 0.03
8.967 ± 0.043
1.976 ± 0.019
5.474 ± 0.029
2.333 ± 0.029
10.499 ± 0.048
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.489 ± 0.02
2.439 ± 0.021
5.307 ± 0.033
2.885 ± 0.023
7.34 ± 0.041
5.188 ± 0.028
5.319 ± 0.026
7.439 ± 0.037
1.256 ± 0.017
2.137 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here