Labedella phragmitis
Average proteome isoelectric point is 5.9
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3187 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3S3ZHZ2|A0A3S3ZHZ2_9MICO GNAT family N-acetyltransferase OS=Labedella phragmitis OX=2498849 GN=ELQ90_13645 PE=4 SV=1
MM1 pKa = 7.1 TRR3 pKa = 11.84 HH4 pKa = 5.48 HH5 pKa = 7.22 RR6 pKa = 11.84 NRR8 pKa = 11.84 LTATLAAATASLLVLAGCAGGAGSTGAAPDD38 pKa = 3.7 ANAEE42 pKa = 4.29 IIVGSQNEE50 pKa = 4.44 PTNLDD55 pKa = 3.56 QIFGGSSGVTEE66 pKa = 4.12 VFTGNVYY73 pKa = 10.69 EE74 pKa = 4.21 GLFRR78 pKa = 11.84 ITDD81 pKa = 3.73 DD82 pKa = 4.8 AEE84 pKa = 4.27 VEE86 pKa = 4.08 PLLAAEE92 pKa = 4.68 TEE94 pKa = 4.21 VSEE97 pKa = 6.06 DD98 pKa = 2.95 GLTYY102 pKa = 10.72 TFTLQDD108 pKa = 3.3 ATFHH112 pKa = 6.35 SGAPLTAEE120 pKa = 3.75 AVKK123 pKa = 10.85 YY124 pKa = 10.26 SLEE127 pKa = 3.97 RR128 pKa = 11.84 FIGEE132 pKa = 3.63 EE133 pKa = 3.86 SIAARR138 pKa = 11.84 KK139 pKa = 8.85 SQLSVIDD146 pKa = 3.63 TVTAVDD152 pKa = 3.96 DD153 pKa = 3.81 KK154 pKa = 10.83 TVEE157 pKa = 4.13 VTLSSKK163 pKa = 10.83 SISFTYY169 pKa = 10.02 NLGYY173 pKa = 10.4 VWIVNPEE180 pKa = 4.11 AGDD183 pKa = 3.45 LTAAADD189 pKa = 3.82 GTGPYY194 pKa = 10.49 SLGDD198 pKa = 3.57 YY199 pKa = 10.5 RR200 pKa = 11.84 KK201 pKa = 9.9 GDD203 pKa = 4.01 SITLEE208 pKa = 4.02 VNDD211 pKa = 5.51 DD212 pKa = 3.62 YY213 pKa = 11.6 WGDD216 pKa = 3.49 APANGGVVYY225 pKa = 10.11 QYY227 pKa = 11.22 YY228 pKa = 11.08 ADD230 pKa = 3.7 PTALNNALVTGAVDD244 pKa = 4.82 LVTSQSNPDD253 pKa = 3.23 SLAEE257 pKa = 4.12 FEE259 pKa = 4.48 SGDD262 pKa = 3.61 YY263 pKa = 10.69 EE264 pKa = 4.57 IIEE267 pKa = 4.45 GTSTTKK273 pKa = 10.62 EE274 pKa = 3.52 LLAFNDD280 pKa = 3.51 RR281 pKa = 11.84 VAPFDD286 pKa = 3.85 DD287 pKa = 3.85 VNVRR291 pKa = 11.84 KK292 pKa = 9.48 AVYY295 pKa = 9.83 SAIDD299 pKa = 3.45 RR300 pKa = 11.84 EE301 pKa = 4.17 QLLDD305 pKa = 4.88 AIWDD309 pKa = 3.71 GRR311 pKa = 11.84 GQLIGSMVPPSEE323 pKa = 3.77 PWYY326 pKa = 11.0 LDD328 pKa = 3.57 LADD331 pKa = 4.62 NNPYY335 pKa = 10.66 DD336 pKa = 4.35 PEE338 pKa = 5.05 LSADD342 pKa = 3.91 LLADD346 pKa = 3.9 AGYY349 pKa = 11.41 ADD351 pKa = 4.58 GFSFTIDD358 pKa = 3.43 TPDD361 pKa = 3.39 SGVHH365 pKa = 4.16 STVAEE370 pKa = 4.23 FLQTEE375 pKa = 4.35 LAKK378 pKa = 11.04 VGVTVDD384 pKa = 3.22 INIITDD390 pKa = 3.59 DD391 pKa = 3.08 EE392 pKa = 4.66 WYY394 pKa = 9.77 EE395 pKa = 4.36 KK396 pKa = 10.96 VYY398 pKa = 10.54 TDD400 pKa = 4.27 HH401 pKa = 7.53 DD402 pKa = 4.28 FEE404 pKa = 4.72 ATLQGHH410 pKa = 5.63 VNDD413 pKa = 4.96 RR414 pKa = 11.84 DD415 pKa = 3.37 INFYY419 pKa = 11.17 GNPDD423 pKa = 4.29 FYY425 pKa = 10.64 WGYY428 pKa = 11.34 DD429 pKa = 3.48 DD430 pKa = 6.71 AEE432 pKa = 4.43 VQGWLAEE439 pKa = 4.06 AEE441 pKa = 4.34 QADD444 pKa = 4.06 STEE447 pKa = 4.06 QQTEE451 pKa = 4.68 LITQVNQKK459 pKa = 9.86 ISDD462 pKa = 3.87 DD463 pKa = 3.84 AASVWLYY470 pKa = 10.76 LNPQLRR476 pKa = 11.84 IVRR479 pKa = 11.84 DD480 pKa = 4.5 GITGVPEE487 pKa = 3.93 NGLNSLFYY495 pKa = 11.03 VYY497 pKa = 10.23 DD498 pKa = 3.43 IQEE501 pKa = 3.75 AA502 pKa = 3.49
Molecular weight: 54.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.727
IPC2_protein 3.783
IPC_protein 3.808
Toseland 3.592
ProMoST 3.961
Dawson 3.795
Bjellqvist 3.948
Wikipedia 3.719
Rodwell 3.63
Grimsley 3.503
Solomon 3.795
Lehninger 3.745
Nozaki 3.897
DTASelect 4.139
Thurlkill 3.63
EMBOSS 3.732
Sillero 3.935
Patrickios 1.875
IPC_peptide 3.795
IPC2_peptide 3.91
IPC2.peptide.svr19 3.826
Protein with the highest isoelectric point:
>tr|A0A444PUM2|A0A444PUM2_9MICO DUF664 domain-containing protein OS=Labedella phragmitis OX=2498849 GN=ELQ90_05520 PE=4 SV=1
MM1 pKa = 7.4 GSVIKK6 pKa = 10.42 KK7 pKa = 8.47 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.22 RR11 pKa = 11.84 MAKK14 pKa = 9.41 KK15 pKa = 9.87 KK16 pKa = 9.7 HH17 pKa = 5.84 RR18 pKa = 11.84 KK19 pKa = 8.56 LLRR22 pKa = 11.84 KK23 pKa = 7.78 TRR25 pKa = 11.84 HH26 pKa = 3.65 QRR28 pKa = 11.84 RR29 pKa = 11.84 NKK31 pKa = 9.78 KK32 pKa = 9.85
Molecular weight: 4.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.493
IPC2_protein 11.14
IPC_protein 12.691
Toseland 12.866
ProMoST 13.349
Dawson 12.866
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.735
Grimsley 12.91
Solomon 13.349
Lehninger 13.261
Nozaki 12.866
DTASelect 12.852
Thurlkill 12.866
EMBOSS 13.364
Sillero 12.866
Patrickios 12.457
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.071
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3187
0
3187
1053170
32
3093
330.5
35.26
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.974 ± 0.056
0.459 ± 0.01
6.523 ± 0.045
5.792 ± 0.046
3.169 ± 0.028
9.033 ± 0.036
1.876 ± 0.021
4.48 ± 0.031
1.757 ± 0.029
9.816 ± 0.055
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.645 ± 0.019
1.918 ± 0.025
5.381 ± 0.027
2.378 ± 0.026
7.334 ± 0.058
6.276 ± 0.034
6.349 ± 0.05
9.486 ± 0.042
1.411 ± 0.016
1.942 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here