Oharaeibacter diazotrophicus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Pleomorphomonadaceae; Oharaeibacter

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4501 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R6RGY1|A0A4R6RGY1_9RHIZ Uncharacterized protein OS=Oharaeibacter diazotrophicus OX=1920512 GN=EDD54_2445 PE=4 SV=1
MM1 pKa = 7.86IDD3 pKa = 4.77LDD5 pKa = 4.11PEE7 pKa = 4.39TEE9 pKa = 3.8IEE11 pKa = 4.58ALIEE15 pKa = 4.22TYY17 pKa = 10.85AGGFDD22 pKa = 3.75DD23 pKa = 5.58HH24 pKa = 8.75DD25 pKa = 5.4GEE27 pKa = 5.32AVTACFAYY35 pKa = 9.48PAVIWQFGIGHH46 pKa = 6.27VFADD50 pKa = 3.82ADD52 pKa = 3.68EE53 pKa = 4.27MAEE56 pKa = 4.49NIAALLKK63 pKa = 9.26VHH65 pKa = 6.66EE66 pKa = 4.46EE67 pKa = 3.81AGIVLSSFEE76 pKa = 4.26IVTLAVSGTAALATVEE92 pKa = 4.18WEE94 pKa = 4.06QEE96 pKa = 3.77DD97 pKa = 4.19DD98 pKa = 3.85EE99 pKa = 5.79GVVLMDD105 pKa = 4.08FTCHH109 pKa = 5.67YY110 pKa = 11.26ALIRR114 pKa = 11.84QGDD117 pKa = 3.68RR118 pKa = 11.84WRR120 pKa = 11.84IASVVNVEE128 pKa = 4.35EE129 pKa = 5.37DD130 pKa = 4.18DD131 pKa = 4.95DD132 pKa = 6.77GEE134 pKa = 5.87DD135 pKa = 3.56EE136 pKa = 4.85DD137 pKa = 4.91

Molecular weight: 15.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R6RCR7|A0A4R6RCR7_9RHIZ Uncharacterized protein DUF1127 OS=Oharaeibacter diazotrophicus OX=1920512 GN=EDD54_3459 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVGGRR28 pKa = 11.84KK29 pKa = 9.3VIARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AQGRR39 pKa = 11.84KK40 pKa = 9.16RR41 pKa = 11.84LSAA44 pKa = 3.93

Molecular weight: 5.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4501

0

4501

1466482

27

2936

325.8

34.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

15.14 ± 0.075

0.73 ± 0.012

6.132 ± 0.027

5.332 ± 0.033

3.498 ± 0.024

9.226 ± 0.035

1.858 ± 0.018

4.195 ± 0.025

2.487 ± 0.033

9.866 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.071 ± 0.017

1.899 ± 0.02

5.622 ± 0.032

2.095 ± 0.021

7.95 ± 0.044

4.438 ± 0.027

5.436 ± 0.036

8.817 ± 0.037

1.25 ± 0.015

1.957 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski