Spiroplasma phage SVTS2

Taxonomy: Viruses; Monodnaviria; Loebvirae; Hofneiviricota; Faserviricetes; Tubulavirales; Plectroviridae; Suturavirus; Spiroplasma virus SVTS2

Average proteome isoelectric point is 7.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 13 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q9QTH6|Q9QTH6_9VIRU ORF D OS=Spiroplasma phage SVTS2 OX=93224 PE=4 SV=1
MM1 pKa = 7.66NGFLVQLIDD10 pKa = 3.2IATGKK15 pKa = 9.95IIKK18 pKa = 9.43DD19 pKa = 3.48DD20 pKa = 4.02KK21 pKa = 11.25GNEE24 pKa = 4.43SKK26 pKa = 10.18WDD28 pKa = 3.64SYY30 pKa = 11.58TFTPVIKK37 pKa = 10.74LEE39 pKa = 4.13NGTVKK44 pKa = 9.52GTKK47 pKa = 9.82DD48 pKa = 3.18LSKK51 pKa = 11.15SKK53 pKa = 9.53WFKK56 pKa = 10.08ITDD59 pKa = 3.47EE60 pKa = 4.73NYY62 pKa = 10.99LEE64 pKa = 4.38LKK66 pKa = 10.28PYY68 pKa = 10.57LIDD71 pKa = 4.3GNLFYY76 pKa = 11.35VSLKK80 pKa = 9.6WDD82 pKa = 3.37GKK84 pKa = 9.76INIVEE89 pKa = 4.76PYY91 pKa = 8.58TEE93 pKa = 4.42NYY95 pKa = 9.88NEE97 pKa = 3.73QEE99 pKa = 4.5FINKK103 pKa = 9.33YY104 pKa = 10.24SNNSSITEE112 pKa = 4.25SNSS115 pKa = 2.92

Molecular weight: 13.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q9QTH9|Q9QTH9_9VIRU ORF 1 OS=Spiroplasma phage SVTS2 OX=93224 PE=4 SV=1
MM1 pKa = 7.41NNVFIQDD8 pKa = 3.2NLTNKK13 pKa = 8.97RR14 pKa = 11.84HH15 pKa = 4.95NVFMNALNYY24 pKa = 9.36VKK26 pKa = 10.46KK27 pKa = 10.3EE28 pKa = 3.91YY29 pKa = 10.14YY30 pKa = 9.33LKK32 pKa = 10.68KK33 pKa = 10.3VFYY36 pKa = 10.81GNYY39 pKa = 8.54VKK41 pKa = 10.92NIVLPLEE48 pKa = 4.24LVNNNPRR55 pKa = 11.84NKK57 pKa = 9.72MGVKK61 pKa = 8.77NTGKK65 pKa = 9.97NDD67 pKa = 3.18KK68 pKa = 10.23KK69 pKa = 10.63LWNSRR74 pKa = 11.84IRR76 pKa = 11.84SQGNCIRR83 pKa = 11.84KK84 pKa = 9.65AYY86 pKa = 9.92EE87 pKa = 3.92NFWNCKK93 pKa = 8.53NLSFLTLTYY102 pKa = 10.78AVNEE106 pKa = 4.15KK107 pKa = 10.35DD108 pKa = 4.1VKK110 pKa = 10.52KK111 pKa = 10.81CKK113 pKa = 10.11NDD115 pKa = 3.15LKK117 pKa = 11.35LFFNNINRR125 pKa = 11.84WWNNPIRR132 pKa = 11.84SKK134 pKa = 9.03NHH136 pKa = 5.59KK137 pKa = 10.39GILKK141 pKa = 10.07YY142 pKa = 10.0MYY144 pKa = 9.1TYY146 pKa = 10.19EE147 pKa = 4.07YY148 pKa = 9.64QKK150 pKa = 10.27RR151 pKa = 11.84GAVHH155 pKa = 5.8FHH157 pKa = 6.99IILNQKK163 pKa = 9.0IPNSVVQQYY172 pKa = 7.72WKK174 pKa = 10.74HH175 pKa = 6.02GINKK179 pKa = 9.01NIKK182 pKa = 8.71VRR184 pKa = 11.84AGSNEE189 pKa = 3.88DD190 pKa = 3.38VVKK193 pKa = 10.98YY194 pKa = 8.98LAKK197 pKa = 10.85YY198 pKa = 9.05IVKK201 pKa = 8.76TANNDD206 pKa = 2.9KK207 pKa = 10.81SQNHH211 pKa = 5.95YY212 pKa = 10.84DD213 pKa = 4.01LNIKK217 pKa = 10.06AYY219 pKa = 10.18QFSKK223 pKa = 10.1NCKK226 pKa = 7.56NPKK229 pKa = 9.41VKK231 pKa = 10.71VGVIEE236 pKa = 5.62LSEE239 pKa = 3.83QDD241 pKa = 4.22LIYY244 pKa = 10.71SVKK247 pKa = 10.97DD248 pKa = 3.17NLNYY252 pKa = 10.22FSFCDD257 pKa = 3.4KK258 pKa = 10.8NGYY261 pKa = 9.71KK262 pKa = 9.74IGFSCDD268 pKa = 2.72SYY270 pKa = 11.66YY271 pKa = 11.44GLDD274 pKa = 3.31KK275 pKa = 10.79FRR277 pKa = 11.84EE278 pKa = 4.16FKK280 pKa = 10.71KK281 pKa = 10.86YY282 pKa = 10.26VSADD286 pKa = 3.24RR287 pKa = 11.84KK288 pKa = 9.98IFRR291 pKa = 11.84NLVKK295 pKa = 9.97NTNFTVNKK303 pKa = 7.85NTNLRR308 pKa = 11.84CC309 pKa = 3.83

Molecular weight: 36.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13

0

13

1810

52

394

139.2

16.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

2.762 ± 0.649

1.271 ± 0.481

5.304 ± 0.614

3.204 ± 0.521

8.122 ± 1.074

4.199 ± 0.459

1.271 ± 0.378

11.16 ± 1.184

9.724 ± 1.151

9.669 ± 0.665

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.818 ± 0.363

9.503 ± 1.209

1.989 ± 0.252

2.818 ± 0.413

2.762 ± 0.428

5.912 ± 0.709

3.923 ± 0.5

6.243 ± 0.764

1.823 ± 0.232

5.525 ± 0.68
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski