Arabidopsis thaliana (Mouse-ear cress)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 41612 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F4I456|F4I456_ARATH Tetratricopeptide repeat (TPR)-like superfamily protein OS=Arabidopsis thaliana OX=3702 GN=TPR2 PE=1 SV=1
MM1 pKa = 7.53LNIIINVMEE10 pKa = 5.03IIWRR14 pKa = 11.84TIVSTLMLFFFLRR27 pKa = 11.84YY28 pKa = 9.41FNNPLLLFFFLCTILFLLIIDD49 pKa = 4.5RR50 pKa = 11.84NRR52 pKa = 11.84SVYY55 pKa = 10.72EE56 pKa = 3.85EE57 pKa = 4.07TGDD60 pKa = 4.05FYY62 pKa = 11.94VLYY65 pKa = 9.88TFQEE69 pKa = 4.43DD70 pKa = 3.36NMLDD74 pKa = 3.71GEE76 pKa = 4.83IIDD79 pKa = 5.17KK80 pKa = 10.78NRR82 pKa = 11.84EE83 pKa = 3.94CCDD86 pKa = 3.33FTRR89 pKa = 11.84YY90 pKa = 10.44YY91 pKa = 11.18YY92 pKa = 10.38DD93 pKa = 4.07DD94 pKa = 4.16CFSYY98 pKa = 11.48YY99 pKa = 10.78DD100 pKa = 4.29GFDD103 pKa = 3.26EE104 pKa = 5.18VDD106 pKa = 3.31NDD108 pKa = 3.86GCIWNDD114 pKa = 3.8DD115 pKa = 3.95EE116 pKa = 6.99DD117 pKa = 5.6FSQCNQDD124 pKa = 3.85GGDD127 pKa = 3.89DD128 pKa = 4.43GIEE131 pKa = 4.42NRR133 pKa = 11.84DD134 pKa = 3.54DD135 pKa = 4.86DD136 pKa = 5.69DD137 pKa = 3.66NHH139 pKa = 7.87AYY141 pKa = 10.41GDD143 pKa = 4.23DD144 pKa = 3.79VDD146 pKa = 6.17DD147 pKa = 4.54DD148 pKa = 4.44TLTSRR153 pKa = 11.84IEE155 pKa = 4.12DD156 pKa = 3.93YY157 pKa = 10.72IANVYY162 pKa = 9.72NGWRR166 pKa = 11.84EE167 pKa = 3.68EE168 pKa = 3.87RR169 pKa = 11.84RR170 pKa = 11.84TDD172 pKa = 3.4KK173 pKa = 11.35LFVV176 pKa = 3.43

Molecular weight: 21.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P93014|RR5_ARATH 30S ribosomal protein S5 chloroplastic OS=Arabidopsis thaliana OX=3702 GN=rps5 PE=2 SV=1
MM1 pKa = 7.1RR2 pKa = 11.84AKK4 pKa = 9.27WKK6 pKa = 9.53KK7 pKa = 9.88KK8 pKa = 8.64RR9 pKa = 11.84MRR11 pKa = 11.84RR12 pKa = 11.84LKK14 pKa = 10.08RR15 pKa = 11.84KK16 pKa = 8.21RR17 pKa = 11.84RR18 pKa = 11.84KK19 pKa = 8.46MRR21 pKa = 11.84QRR23 pKa = 11.84SKK25 pKa = 11.41

Molecular weight: 3.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

27468

14144

41612

17599415

5

5400

422.9

47.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.272 ± 0.011

1.847 ± 0.006

5.405 ± 0.009

6.757 ± 0.016

4.259 ± 0.008

6.354 ± 0.013

2.279 ± 0.005

5.327 ± 0.01

6.367 ± 0.012

9.593 ± 0.016

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.442 ± 0.004

4.396 ± 0.007

4.784 ± 0.014

3.551 ± 0.008

5.385 ± 0.009

9.194 ± 0.013

5.08 ± 0.008

6.644 ± 0.008

1.235 ± 0.004

2.828 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski