Corynebacterium freneyi DNF00450

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Corynebacteriaceae; Corynebacterium; Corynebacterium freneyi

Average proteome isoelectric point is 5.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1898 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A095ZBY3|A0A095ZBY3_9CORY Queuine tRNA-ribosyltransferase OS=Corynebacterium freneyi DNF00450 OX=1287475 GN=tgt PE=3 SV=1
MM1 pKa = 7.28KK2 pKa = 10.35RR3 pKa = 11.84SNTLAALVLTSTLALTACSDD23 pKa = 3.52ATDD26 pKa = 3.89NSDD29 pKa = 4.51DD30 pKa = 4.35AGTTSTTTATAEE42 pKa = 4.19TNEE45 pKa = 4.09THH47 pKa = 7.0QEE49 pKa = 3.95DD50 pKa = 4.6TTTADD55 pKa = 4.03DD56 pKa = 4.51GGHH59 pKa = 6.68DD60 pKa = 3.93MEE62 pKa = 6.45HH63 pKa = 7.26PEE65 pKa = 5.16DD66 pKa = 4.54GGPPPEE72 pKa = 5.59GITEE76 pKa = 4.31ATDD79 pKa = 3.12PTYY82 pKa = 10.54PVGSEE87 pKa = 3.95VMLTADD93 pKa = 4.71HH94 pKa = 6.35MPGMDD99 pKa = 3.56GAQATISGAFDD110 pKa = 3.15TTTYY114 pKa = 10.32SVSYY118 pKa = 9.4TPTDD122 pKa = 3.06GGEE125 pKa = 4.04PVTDD129 pKa = 3.63HH130 pKa = 6.41RR131 pKa = 11.84WVVHH135 pKa = 6.18EE136 pKa = 4.37EE137 pKa = 3.87LVDD140 pKa = 4.11PGEE143 pKa = 4.35APLPGGTEE151 pKa = 3.84VVLNAEE157 pKa = 4.93HH158 pKa = 6.23MPGMQGATATIDD170 pKa = 3.31YY171 pKa = 7.46STQEE175 pKa = 3.84TVYY178 pKa = 10.22MVDD181 pKa = 2.89IDD183 pKa = 3.97TGEE186 pKa = 3.84MTMINHH192 pKa = 6.4KK193 pKa = 9.56WVTEE197 pKa = 4.09SEE199 pKa = 4.23IQPAEE204 pKa = 3.8

Molecular weight: 21.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A095Y4F5|A0A095Y4F5_9CORY Branched-chain amino acid ABC transporter permease OS=Corynebacterium freneyi DNF00450 OX=1287475 GN=HMPREF1650_05260 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84ARR15 pKa = 11.84VHH17 pKa = 5.99GFRR20 pKa = 11.84TRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84SGRR29 pKa = 11.84AVVAARR35 pKa = 11.84RR36 pKa = 11.84NKK38 pKa = 9.36GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1898

0

1898

622226

40

3067

327.8

35.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.751 ± 0.087

0.714 ± 0.016

7.137 ± 0.058

6.314 ± 0.059

2.913 ± 0.034

9.398 ± 0.055

2.097 ± 0.027

4.51 ± 0.039

2.712 ± 0.046

9.142 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.277 ± 0.024

2.284 ± 0.03

5.358 ± 0.043

2.525 ± 0.033

7.148 ± 0.056

5.125 ± 0.04

5.689 ± 0.037

8.575 ± 0.057

1.405 ± 0.027

1.921 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski