Feline coronavirus UU30

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae; Alphacoronavirus; Tegacovirus; Alphacoronavirus 1; Feline coronavirus

Average proteome isoelectric point is 6.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E3W5J2|E3W5J2_9ALPC Non-structural protein 3b OS=Feline coronavirus UU30 OX=906886 PE=4 SV=1
MM1 pKa = 7.71DD2 pKa = 3.9TVKK5 pKa = 10.85SIGISVDD12 pKa = 3.2AVLDD16 pKa = 3.96EE17 pKa = 4.93LDD19 pKa = 4.3SIAFAVTLKK28 pKa = 11.01VLFNPGKK35 pKa = 10.47LLVCIGFGDD44 pKa = 3.81TFEE47 pKa = 4.33EE48 pKa = 4.75AEE50 pKa = 4.06QKK52 pKa = 10.84AYY54 pKa = 10.67AKK56 pKa = 10.13LQLDD60 pKa = 3.79IEE62 pKa = 4.35EE63 pKa = 5.0SPNSTTVV70 pKa = 2.63

Molecular weight: 7.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E3W5J3|E3W5J3_9ALPC Envelope small membrane protein OS=Feline coronavirus UU30 OX=906886 GN=E PE=3 SV=1
MM1 pKa = 7.85PSFSWILKK9 pKa = 9.28SRR11 pKa = 11.84LILRR15 pKa = 11.84LFNITVYY22 pKa = 10.61DD23 pKa = 4.52FCAKK27 pKa = 9.51NWYY30 pKa = 9.24KK31 pKa = 11.03LPFAVRR37 pKa = 11.84LRR39 pKa = 11.84IINNTKK45 pKa = 10.0PKK47 pKa = 8.73TASTIKK53 pKa = 9.52RR54 pKa = 11.84RR55 pKa = 11.84KK56 pKa = 9.36RR57 pKa = 11.84VVPDD61 pKa = 3.16YY62 pKa = 11.22RR63 pKa = 11.84KK64 pKa = 9.67IAILNAARR72 pKa = 11.84KK73 pKa = 9.03

Molecular weight: 8.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10

0

10

13362

70

6696

1336.2

149.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.294 ± 0.191

3.076 ± 0.316

5.793 ± 0.233

4.565 ± 0.267

5.583 ± 0.136

6.152 ± 0.257

1.759 ± 0.207

5.396 ± 0.284

6.601 ± 0.635

8.599 ± 0.618

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.215 ± 0.224

5.822 ± 0.29

3.33 ± 0.165

2.986 ± 0.612

3.076 ± 0.698

6.706 ± 0.389

6.324 ± 0.372

9.759 ± 0.775

1.19 ± 0.177

4.775 ± 0.278
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski