Sanguibacter antarcticus
Average proteome isoelectric point is 5.86
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3108 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2A9E8J7|A0A2A9E8J7_9MICO Two-component system sensor histidine kinase SenX3 OS=Sanguibacter antarcticus OX=372484 GN=ATL42_3210 PE=4 SV=1
MM1 pKa = 7.79 EE2 pKa = 4.89 SMKK5 pKa = 10.67 HH6 pKa = 3.9 STTPRR11 pKa = 11.84 RR12 pKa = 11.84 VLHH15 pKa = 6.06 ALCLGSLVLALTACEE30 pKa = 4.11 DD31 pKa = 3.38 VRR33 pKa = 11.84 AIPWPAAGTGTATDD47 pKa = 3.81 SATADD52 pKa = 3.56 ATPEE56 pKa = 3.96 PSPVQSADD64 pKa = 3.36 LEE66 pKa = 4.39 ATTDD70 pKa = 3.29 EE71 pKa = 4.67 GTAEE75 pKa = 4.14 PVEE78 pKa = 4.41 VAVPDD83 pKa = 4.12 DD84 pKa = 4.03 VVPAPVPAALPEE96 pKa = 4.22 DD97 pKa = 4.11 VVPDD101 pKa = 3.91 EE102 pKa = 4.87 AAPAEE107 pKa = 4.24 PVVVEE112 pKa = 4.64 PVATAEE118 pKa = 4.43 VVVSAAYY125 pKa = 9.65 PDD127 pKa = 4.42 DD128 pKa = 4.47 AVGDD132 pKa = 3.98 YY133 pKa = 10.79 QLGGSYY139 pKa = 9.48 TPPAGVNLVVRR150 pKa = 11.84 DD151 pKa = 4.13 STSKK155 pKa = 10.21 PAPGLYY161 pKa = 9.99 NICYY165 pKa = 9.9 INGFQTQSDD174 pKa = 4.65 DD175 pKa = 3.32 SDD177 pKa = 3.46 AWLKK181 pKa = 10.45 DD182 pKa = 3.2 HH183 pKa = 7.52 PEE185 pKa = 4.05 LVLHH189 pKa = 6.71 VDD191 pKa = 4.33 GEE193 pKa = 4.61 PVADD197 pKa = 4.46 PGWPDD202 pKa = 3.32 EE203 pKa = 4.09 YY204 pKa = 11.5 LLDD207 pKa = 3.49 VSTAAKK213 pKa = 8.24 RR214 pKa = 11.84 TAIADD219 pKa = 3.36 IMAVTMTSCADD230 pKa = 3.19 KK231 pKa = 11.2 GFDD234 pKa = 5.01 AIEE237 pKa = 4.05 FDD239 pKa = 4.58 NLDD242 pKa = 3.44 SYY244 pKa = 11.5 LRR246 pKa = 11.84 SDD248 pKa = 4.29 DD249 pKa = 5.19 ALDD252 pKa = 3.82 IDD254 pKa = 5.15 DD255 pKa = 5.02 AVATARR261 pKa = 11.84 LYY263 pKa = 9.57 TEE265 pKa = 4.81 RR266 pKa = 11.84 GHH268 pKa = 7.53 ALGLEE273 pKa = 4.33 VGQKK277 pKa = 8.16 NTAEE281 pKa = 4.16 LGSRR285 pKa = 11.84 GRR287 pKa = 11.84 DD288 pKa = 3.05 EE289 pKa = 4.61 VGFDD293 pKa = 3.04 FAIAEE298 pKa = 4.12 EE299 pKa = 4.33 CGVYY303 pKa = 9.88 EE304 pKa = 4.28 EE305 pKa = 5.57 CSDD308 pKa = 3.72 FTDD311 pKa = 3.66 VYY313 pKa = 10.14 GSHH316 pKa = 6.18 VFAIEE321 pKa = 3.89 YY322 pKa = 10.41 PEE324 pKa = 6.13 DD325 pKa = 3.68 GDD327 pKa = 4.16 FAEE330 pKa = 5.29 SCASPGRR337 pKa = 11.84 PDD339 pKa = 4.29 NMILRR344 pKa = 11.84 DD345 pKa = 4.09 LDD347 pKa = 4.07 LVTPSDD353 pKa = 3.11 SDD355 pKa = 3.76 YY356 pKa = 11.52 VYY358 pKa = 10.73 EE359 pKa = 4.18 RR360 pKa = 11.84 CC361 pKa = 4.98
Molecular weight: 38.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.689
IPC2_protein 3.783
IPC_protein 3.808
Toseland 3.592
ProMoST 3.961
Dawson 3.795
Bjellqvist 3.948
Wikipedia 3.719
Rodwell 3.63
Grimsley 3.49
Solomon 3.795
Lehninger 3.745
Nozaki 3.897
DTASelect 4.151
Thurlkill 3.63
EMBOSS 3.732
Sillero 3.923
Patrickios 1.367
IPC_peptide 3.783
IPC2_peptide 3.91
IPC2.peptide.svr19 3.823
Protein with the highest isoelectric point:
>tr|A0A2A9E3D3|A0A2A9E3D3_9MICO Ribosomal silencing factor RsfS OS=Sanguibacter antarcticus OX=372484 GN=rsfS PE=3 SV=1
MM1 pKa = 7.96 PSPLLRR7 pKa = 11.84 LARR10 pKa = 11.84 SASRR14 pKa = 11.84 QHH16 pKa = 5.82 STSSPSSPRR25 pKa = 11.84 LRR27 pKa = 11.84 PSPPSPPSVAVRR39 pKa = 11.84 RR40 pKa = 11.84 PPDD43 pKa = 4.07 GPPPAPQFSTPLCPTRR59 pKa = 11.84 SSPPPQRR66 pKa = 11.84 RR67 pKa = 11.84 PALPRR72 pKa = 11.84 PTRR75 pKa = 11.84 SGPRR79 pKa = 11.84 RR80 pKa = 11.84 RR81 pKa = 11.84 AAAGHH86 pKa = 5.53 EE87 pKa = 4.02 RR88 pKa = 11.84 RR89 pKa = 11.84 ARR91 pKa = 11.84 RR92 pKa = 11.84 PGTPRR97 pKa = 11.84 RR98 pKa = 11.84 LTTTRR103 pKa = 11.84 LPRR106 pKa = 11.84 STWSPLSRR114 pKa = 11.84 RR115 pKa = 11.84 SHH117 pKa = 6.5 HH118 pKa = 6.28 GRR120 pKa = 11.84 LPTTPLHH127 pKa = 6.63 SPLAHH132 pKa = 6.98 AGHH135 pKa = 7.32 AVQAARR141 pKa = 11.84 RR142 pKa = 11.84 ARR144 pKa = 11.84 PPSRR148 pKa = 11.84 RR149 pKa = 11.84 LHH151 pKa = 6.42 RR152 pKa = 11.84 AFQRR156 pKa = 11.84 HH157 pKa = 5.07 RR158 pKa = 11.84 ALRR161 pKa = 11.84 WLLLSRR167 pKa = 11.84 LSSSSRR173 pKa = 11.84 LSRR176 pKa = 11.84 RR177 pKa = 11.84 RR178 pKa = 11.84 PLSRR182 pKa = 11.84 RR183 pKa = 11.84 SPASRR188 pKa = 11.84 VARR191 pKa = 11.84 PAPGGAVEE199 pKa = 4.25 PRR201 pKa = 11.84 ARR203 pKa = 11.84 PARR206 pKa = 11.84 RR207 pKa = 11.84 RR208 pKa = 11.84 PRR210 pKa = 11.84 PPPSPRR216 pKa = 11.84 APLRR220 pKa = 11.84 SPNRR224 pKa = 11.84 TVHH227 pKa = 6.04 RR228 pKa = 11.84 MLRR231 pKa = 11.84 RR232 pKa = 11.84 RR233 pKa = 11.84 RR234 pKa = 11.84 PTPGQMLQGAPRR246 pKa = 11.84 PQRR249 pKa = 11.84 ALQTQTALLLQRR261 pKa = 11.84 SLRR264 pKa = 11.84 PHH266 pKa = 6.07 ARR268 pKa = 11.84 AGRR271 pKa = 11.84 GG272 pKa = 3.2
Molecular weight: 30.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.301
IPC_protein 12.91
Toseland 13.071
ProMoST 13.568
Dawson 13.071
Bjellqvist 13.071
Wikipedia 13.554
Rodwell 12.574
Grimsley 13.115
Solomon 13.568
Lehninger 13.466
Nozaki 13.071
DTASelect 13.071
Thurlkill 13.071
EMBOSS 13.568
Sillero 13.071
Patrickios 12.281
IPC_peptide 13.568
IPC2_peptide 12.559
IPC2.peptide.svr19 9.254
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3108
0
3108
1047096
30
2351
336.9
35.82
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.362 ± 0.064
0.625 ± 0.011
6.428 ± 0.038
5.43 ± 0.036
2.699 ± 0.028
8.915 ± 0.037
2.121 ± 0.023
3.732 ± 0.035
1.681 ± 0.032
10.183 ± 0.057
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.769 ± 0.019
1.709 ± 0.024
5.499 ± 0.033
2.739 ± 0.024
7.15 ± 0.046
6.024 ± 0.034
6.789 ± 0.044
9.859 ± 0.048
1.419 ± 0.018
1.868 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here