Amino acid dipepetide frequency for bacterium HR19

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	1.609AlaAla: 1.609 ± 0.072	0.56AlaCys: 0.56 ± 0.036	2.663AlaAsp: 2.663 ± 0.062	4.795AlaGlu: 4.795 ± 0.107	3.068AlaPhe: 3.068 ± 0.083	4.025AlaGly: 4.025 ± 0.104	0.77AlaHis: 0.77 ± 0.038	4.985AlaIle: 4.985 ± 0.104	5.017AlaLys: 5.017 ± 0.098	5.311AlaLeu: 5.311 ± 0.106	1.064AlaMet: 1.064 ± 0.052	1.683AlaAsn: 1.683 ± 0.063	1.56AlaPro: 1.56 ± 0.053	1.522AlaGln: 1.522 ± 0.056	2.621AlaArg: 2.621 ± 0.077	3.099AlaSer: 3.099 ± 0.082	2.018AlaThr: 2.018 ± 0.067	4.037AlaVal: 4.037 ± 0.076	0.477AlaTrp: 0.477 ± 0.029	1.791AlaTyr: 1.791 ± 0.064	0.0AlaXaa: 0.0 ± 0.0
Cys	0.697CysAla: 0.697 ± 0.041	0.101CysCys: 0.101 ± 0.015	0.572CysAsp: 0.572 ± 0.031	0.768CysGlu: 0.768 ± 0.036	0.491CysPhe: 0.491 ± 0.028	0.993CysGly: 0.993 ± 0.045	0.213CysHis: 0.213 ± 0.023	0.652CysIle: 0.652 ± 0.034	0.57CysLys: 0.57 ± 0.034	0.662CysLeu: 0.662 ± 0.031	0.106CysMet: 0.106 ± 0.013	0.317CysAsn: 0.317 ± 0.036	0.565CysPro: 0.565 ± 0.035	0.236CysGln: 0.236 ± 0.02	0.355CysArg: 0.355 ± 0.027	0.853CysSer: 0.853 ± 0.042	0.303CysThr: 0.303 ± 0.023	0.763CysVal: 0.763 ± 0.04	0.116CysTrp: 0.116 ± 0.023	0.295CysTyr: 0.295 ± 0.024	0.0CysXaa: 0.0 ± 0.0
Asp	2.052AspAla: 2.052 ± 0.065	0.459AspCys: 0.459 ± 0.034	2.212AspAsp: 2.212 ± 0.069	4.843AspGlu: 4.843 ± 0.105	4.249AspPhe: 4.249 ± 0.093	3.47AspGly: 3.47 ± 0.099	0.388AspHis: 0.388 ± 0.026	6.152AspIle: 6.152 ± 0.101	3.874AspLys: 3.874 ± 0.081	3.692AspLeu: 3.692 ± 0.083	1.071AspMet: 1.071 ± 0.045	1.524AspAsn: 1.524 ± 0.058	1.857AspPro: 1.857 ± 0.069	0.647AspGln: 0.647 ± 0.035	1.794AspArg: 1.794 ± 0.062	2.782AspSer: 2.782 ± 0.082	1.851AspThr: 1.851 ± 0.066	3.722AspVal: 3.722 ± 0.085	0.541AspTrp: 0.541 ± 0.036	1.772AspTyr: 1.772 ± 0.059	0.0AspXaa: 0.0 ± 0.0
Glu	3.221GluAla: 3.221 ± 0.086	0.517GluCys: 0.517 ± 0.033	3.983GluAsp: 3.983 ± 0.092	8.187GluGlu: 8.187 ± 0.187	4.176GluPhe: 4.176 ± 0.086	5.129GluGly: 5.129 ± 0.099	0.96GluHis: 0.96 ± 0.041	9.362GluIle: 9.362 ± 0.152	11.754GluLys: 11.754 ± 0.201	7.107GluLeu: 7.107 ± 0.129	1.638GluMet: 1.638 ± 0.053	4.627GluAsn: 4.627 ± 0.104	2.186GluPro: 2.186 ± 0.056	1.534GluGln: 1.534 ± 0.055	4.477GluArg: 4.477 ± 0.101	3.89GluSer: 3.89 ± 0.104	2.748GluThr: 2.748 ± 0.077	5.344GluVal: 5.344 ± 0.103	0.638GluTrp: 0.638 ± 0.03	2.238GluTyr: 2.238 ± 0.063	0.0GluXaa: 0.0 ± 0.0
Phe	3.656PheAla: 3.656 ± 0.074	0.692PheCys: 0.692 ± 0.037	3.122PheAsp: 3.122 ± 0.083	4.672PheGlu: 4.672 ± 0.1	5.119PhePhe: 5.119 ± 0.128	4.396PheGly: 4.396 ± 0.096	0.806PheHis: 0.806 ± 0.037	5.739PheIle: 5.739 ± 0.121	3.498PheLys: 3.498 ± 0.081	7.248PheLeu: 7.248 ± 0.16	1.187PheMet: 1.187 ± 0.044	1.652PheAsn: 1.652 ± 0.064	2.85PhePro: 2.85 ± 0.075	1.532PheGln: 1.532 ± 0.048	2.463PheArg: 2.463 ± 0.058	6.771PheSer: 6.771 ± 0.165	2.222PheThr: 2.222 ± 0.061	4.162PheVal: 4.162 ± 0.093	0.686PheTrp: 0.686 ± 0.034	2.349PheTyr: 2.349 ± 0.075	0.0PheXaa: 0.0 ± 0.0
Gly	3.521GlyAla: 3.521 ± 0.095	0.712GlyCys: 0.712 ± 0.04	3.349GlyAsp: 3.349 ± 0.086	5.36GlyGlu: 5.36 ± 0.104	4.179GlyPhe: 4.179 ± 0.068	5.06GlyGly: 5.06 ± 0.115	0.842GlyHis: 0.842 ± 0.04	6.357GlyIle: 6.357 ± 0.133	7.168GlyLys: 7.168 ± 0.117	4.933GlyLeu: 4.933 ± 0.114	1.182GlyMet: 1.182 ± 0.048	2.559GlyAsn: 2.559 ± 0.082	1.39GlyPro: 1.39 ± 0.047	1.279GlyGln: 1.279 ± 0.047	3.186GlyArg: 3.186 ± 0.086	3.41GlySer: 3.41 ± 0.084	2.983GlyThr: 2.983 ± 0.078	5.013GlyVal: 5.013 ± 0.103	0.693GlyTrp: 0.693 ± 0.038	2.415GlyTyr: 2.415 ± 0.068	0.0GlyXaa: 0.0 ± 0.0
His	0.624HisAla: 0.624 ± 0.035	0.121HisCys: 0.121 ± 0.014	0.466HisAsp: 0.466 ± 0.029	0.657HisGlu: 0.657 ± 0.036	0.908HisPhe: 0.908 ± 0.039	0.784HisGly: 0.784 ± 0.037	0.312HisHis: 0.312 ± 0.025	1.617HisIle: 1.617 ± 0.056	0.936HisLys: 0.936 ± 0.044	1.31HisLeu: 1.31 ± 0.048	0.196HisMet: 0.196 ± 0.02	0.454HisAsn: 0.454 ± 0.026	0.801HisPro: 0.801 ± 0.038	0.354HisGln: 0.354 ± 0.026	0.598HisArg: 0.598 ± 0.038	0.853HisSer: 0.853 ± 0.041	0.595HisThr: 0.595 ± 0.034	0.66HisVal: 0.66 ± 0.039	0.121HisTrp: 0.121 ± 0.016	0.445HisTyr: 0.445 ± 0.03	0.0HisXaa: 0.0 ± 0.0
Ile	5.424IleAla: 5.424 ± 0.115	0.971IleCys: 0.971 ± 0.039	4.583IleAsp: 4.583 ± 0.089	7.235IleGlu: 7.235 ± 0.132	6.658IlePhe: 6.658 ± 0.111	5.188IleGly: 5.188 ± 0.108	1.146IleHis: 1.146 ± 0.042	7.925IleIle: 7.925 ± 0.139	8.624IleLys: 8.624 ± 0.147	8.984IleLeu: 8.984 ± 0.14	1.546IleMet: 1.546 ± 0.056	3.652IleAsn: 3.652 ± 0.09	4.481IlePro: 4.481 ± 0.084	2.065IleGln: 2.065 ± 0.062	4.266IleArg: 4.266 ± 0.086	9.052IleSer: 9.052 ± 0.162	4.349IleThr: 4.349 ± 0.094	5.348IleVal: 5.348 ± 0.101	0.874IleTrp: 0.874 ± 0.04	3.502IleTyr: 3.502 ± 0.082	0.0IleXaa: 0.0 ± 0.0
Lys	4.134LysAla: 4.134 ± 0.096	0.553LysCys: 0.553 ± 0.033	5.261LysAsp: 5.261 ± 0.089	8.671LysGlu: 8.671 ± 0.167	4.736LysPhe: 4.736 ± 0.095	5.096LysGly: 5.096 ± 0.084	0.829LysHis: 0.829 ± 0.043	12.094LysIle: 12.094 ± 0.177	10.031LysLys: 10.031 ± 0.192	7.469LysLeu: 7.469 ± 0.113	2.006LysMet: 2.006 ± 0.058	5.219LysAsn: 5.219 ± 0.111	2.772LysPro: 2.772 ± 0.07	1.512LysGln: 1.512 ± 0.055	4.425LysArg: 4.425 ± 0.095	5.271LysSer: 5.271 ± 0.099	3.914LysThr: 3.914 ± 0.082	6.228LysVal: 6.228 ± 0.102	0.716LysTrp: 0.716 ± 0.033	3.06LysTyr: 3.06 ± 0.071	0.0LysXaa: 0.0 ± 0.0
Leu	5.436LeuAla: 5.436 ± 0.092	1.004LeuCys: 1.004 ± 0.046	3.772LeuAsp: 3.772 ± 0.077	6.525LeuGlu: 6.525 ± 0.115	5.45LeuPhe: 5.45 ± 0.127	6.072LeuGly: 6.072 ± 0.105	1.082LeuHis: 1.082 ± 0.041	7.267LeuIle: 7.267 ± 0.134	9.354LeuLys: 9.354 ± 0.154	7.525LeuLeu: 7.525 ± 0.157	1.645LeuMet: 1.645 ± 0.059	3.24LeuAsn: 3.24 ± 0.073	3.959LeuPro: 3.959 ± 0.085	2.039LeuGln: 2.039 ± 0.065	4.051LeuArg: 4.051 ± 0.094	8.355LeuSer: 8.355 ± 0.115	3.488LeuThr: 3.488 ± 0.084	4.744LeuVal: 4.744 ± 0.084	0.856LeuTrp: 0.856 ± 0.043	2.865LeuTyr: 2.865 ± 0.07	0.0LeuXaa: 0.0 ± 0.0
Met	1.037MetAla: 1.037 ± 0.042	0.154MetCys: 0.154 ± 0.014	0.565MetAsp: 0.565 ± 0.027	1.186MetGlu: 1.186 ± 0.045	0.815MetPhe: 0.815 ± 0.037	1.336MetGly: 1.336 ± 0.056	0.262MetHis: 0.262 ± 0.023	1.666MetIle: 1.666 ± 0.054	2.248MetLys: 2.248 ± 0.063	1.544MetLeu: 1.544 ± 0.048	0.48MetMet: 0.48 ± 0.033	0.789MetAsn: 0.789 ± 0.036	0.721MetPro: 0.721 ± 0.037	0.466MetGln: 0.466 ± 0.03	1.477MetArg: 1.477 ± 0.055	1.139MetSer: 1.139 ± 0.045	0.851MetThr: 0.851 ± 0.038	1.056MetVal: 1.056 ± 0.045	0.232MetTrp: 0.232 ± 0.019	0.494MetTyr: 0.494 ± 0.028	0.0MetXaa: 0.0 ± 0.0
Asn	1.789AsnAla: 1.789 ± 0.056	0.452AsnCys: 0.452 ± 0.033	1.182AsnAsp: 1.182 ± 0.054	2.349AsnGlu: 2.349 ± 0.07	3.508AsnPhe: 3.508 ± 0.092	2.193AsnGly: 2.193 ± 0.083	0.537AsnHis: 0.537 ± 0.031	4.339AsnIle: 4.339 ± 0.096	2.373AsnLys: 2.373 ± 0.066	4.698AsnLeu: 4.698 ± 0.101	0.74AsnMet: 0.74 ± 0.042	1.094AsnAsn: 1.094 ± 0.055	2.539AsnPro: 2.539 ± 0.081	1.135AsnGln: 1.135 ± 0.046	1.239AsnArg: 1.239 ± 0.041	2.988AsnSer: 2.988 ± 0.087	1.506AsnThr: 1.506 ± 0.061	2.013AsnVal: 2.013 ± 0.067	0.388AsnTrp: 0.388 ± 0.03	1.347AsnTyr: 1.347 ± 0.046	0.0AsnXaa: 0.0 ± 0.0
Pro	2.134ProAla: 2.134 ± 0.057	0.31ProCys: 0.31 ± 0.023	2.564ProAsp: 2.564 ± 0.078	3.975ProGlu: 3.975 ± 0.082	2.382ProPhe: 2.382 ± 0.06	2.188ProGly: 2.188 ± 0.077	0.751ProHis: 0.751 ± 0.033	2.791ProIle: 2.791 ± 0.073	2.959ProLys: 2.959 ± 0.067	3.422ProLeu: 3.422 ± 0.081	0.527ProMet: 0.527 ± 0.028	1.241ProAsn: 1.241 ± 0.052	2.217ProPro: 2.217 ± 0.106	1.38ProGln: 1.38 ± 0.053	1.44ProArg: 1.44 ± 0.047	3.139ProSer: 3.139 ± 0.083	1.557ProThr: 1.557 ± 0.062	2.494ProVal: 2.494 ± 0.059	0.352ProTrp: 0.352 ± 0.026	1.716ProTyr: 1.716 ± 0.054	0.0ProXaa: 0.0 ± 0.0
Gln	1.189GlnAla: 1.189 ± 0.046	0.151GlnCys: 0.151 ± 0.017	1.477GlnAsp: 1.477 ± 0.056	1.974GlnGlu: 1.974 ± 0.058	0.914GlnPhe: 0.914 ± 0.041	1.279GlnGly: 1.279 ± 0.055	0.231GlnHis: 0.231 ± 0.02	2.307GlnIle: 2.307 ± 0.069	3.098GlnLys: 3.098 ± 0.077	1.466GlnLeu: 1.466 ± 0.049	0.491GlnMet: 0.491 ± 0.03	1.584GlnAsn: 1.584 ± 0.045	0.697GlnPro: 0.697 ± 0.032	0.442GlnGln: 0.442 ± 0.032	1.258GlnArg: 1.258 ± 0.047	1.29GlnSer: 1.29 ± 0.046	1.031GlnThr: 1.031 ± 0.045	1.362GlnVal: 1.362 ± 0.049	0.147GlnTrp: 0.147 ± 0.014	0.551GlnTyr: 0.551 ± 0.031	0.0GlnXaa: 0.0 ± 0.0
Arg	2.758ArgAla: 2.758 ± 0.079	0.421ArgCys: 0.421 ± 0.025	2.271ArgAsp: 2.271 ± 0.059	4.812ArgGlu: 4.812 ± 0.115	2.838ArgPhe: 2.838 ± 0.067	2.832ArgGly: 2.832 ± 0.077	0.447ArgHis: 0.447 ± 0.03	4.058ArgIle: 4.058 ± 0.099	5.341ArgLys: 5.341 ± 0.116	2.962ArgLeu: 2.962 ± 0.084	0.9ArgMet: 0.9 ± 0.037	1.891ArgAsn: 1.891 ± 0.057	1.286ArgPro: 1.286 ± 0.047	0.837ArgGln: 0.837 ± 0.042	2.579ArgArg: 2.579 ± 0.075	2.045ArgSer: 2.045 ± 0.057	1.931ArgThr: 1.931 ± 0.061	3.245ArgVal: 3.245 ± 0.075	0.586ArgTrp: 0.586 ± 0.037	1.758ArgTyr: 1.758 ± 0.06	0.0ArgXaa: 0.0 ± 0.0
Ser	4.85SerAla: 4.85 ± 0.099	0.806SerCys: 0.806 ± 0.039	3.755SerAsp: 3.755 ± 0.098	5.736SerGlu: 5.736 ± 0.102	5.245SerPhe: 5.245 ± 0.127	4.984SerGly: 4.984 ± 0.117	1.116SerHis: 1.116 ± 0.046	5.355SerIle: 5.355 ± 0.104	5.417SerLys: 5.417 ± 0.108	6.845SerLeu: 6.845 ± 0.118	1.082SerMet: 1.082 ± 0.049	2.108SerAsn: 2.108 ± 0.072	3.358SerPro: 3.358 ± 0.097	2.25SerGln: 2.25 ± 0.063	3.006SerArg: 3.006 ± 0.083	5.687SerSer: 5.687 ± 0.151	2.338SerThr: 2.338 ± 0.079	4.538SerVal: 4.538 ± 0.092	0.647SerTrp: 0.647 ± 0.032	2.513SerTyr: 2.513 ± 0.072	0.0SerXaa: 0.0 ± 0.0
Thr	2.387ThrAla: 2.387 ± 0.073	0.447ThrCys: 0.447 ± 0.031	1.659ThrAsp: 1.659 ± 0.052	2.86ThrGlu: 2.86 ± 0.07	2.408ThrPhe: 2.408 ± 0.068	2.994ThrGly: 2.994 ± 0.092	0.747ThrHis: 0.747 ± 0.041	3.047ThrIle: 3.047 ± 0.079	2.947ThrLys: 2.947 ± 0.08	3.831ThrLeu: 3.831 ± 0.086	0.685ThrMet: 0.685 ± 0.035	1.191ThrAsn: 1.191 ± 0.048	2.11ThrPro: 2.11 ± 0.06	1.118ThrGln: 1.118 ± 0.042	1.697ThrArg: 1.697 ± 0.048	2.715ThrSer: 2.715 ± 0.082	1.825ThrThr: 1.825 ± 0.063	2.153ThrVal: 2.153 ± 0.066	0.296ThrTrp: 0.296 ± 0.024	1.362ThrTyr: 1.362 ± 0.048	0.0ThrXaa: 0.0 ± 0.0
Val	3.741ValAla: 3.741 ± 0.083	0.662ValCys: 0.662 ± 0.034	3.12ValAsp: 3.12 ± 0.074	5.32ValGlu: 5.32 ± 0.097	4.793ValPhe: 4.793 ± 0.102	4.308ValGly: 4.308 ± 0.091	0.709ValHis: 0.709 ± 0.035	6.11ValIle: 6.11 ± 0.103	5.724ValLys: 5.724 ± 0.114	5.845ValLeu: 5.845 ± 0.125	1.227ValMet: 1.227 ± 0.049	2.013ValAsn: 2.013 ± 0.06	2.487ValPro: 2.487 ± 0.077	1.465ValGln: 1.465 ± 0.053	3.131ValArg: 3.131 ± 0.077	5.225ValSer: 5.225 ± 0.118	1.357ValThr: 1.357 ± 0.056	4.961ValVal: 4.961 ± 0.112	0.732ValTrp: 0.732 ± 0.04	1.711ValTyr: 1.711 ± 0.058	0.0ValXaa: 0.0 ± 0.0
Trp	0.584TrpAla: 0.584 ± 0.029	0.177TrpCys: 0.177 ± 0.023	0.499TrpAsp: 0.499 ± 0.032	0.818TrpGlu: 0.818 ± 0.045	0.508TrpPhe: 0.508 ± 0.033	0.841TrpGly: 0.841 ± 0.042	0.128TrpHis: 0.128 ± 0.012	0.766TrpIle: 0.766 ± 0.039	0.886TrpLys: 0.886 ± 0.037	0.655TrpLeu: 0.655 ± 0.037	0.175TrpMet: 0.175 ± 0.019	0.445TrpAsn: 0.445 ± 0.026	0.175TrpPro: 0.175 ± 0.019	0.296TrpGln: 0.296 ± 0.023	0.522TrpArg: 0.522 ± 0.031	0.529TrpSer: 0.529 ± 0.028	0.383TrpThr: 0.383 ± 0.028	0.629TrpVal: 0.629 ± 0.033	0.168TrpTrp: 0.168 ± 0.017	0.357TrpTyr: 0.357 ± 0.029	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.832TyrAla: 1.832 ± 0.054	0.357TyrCys: 0.357 ± 0.028	1.877TyrAsp: 1.877 ± 0.055	2.975TyrGlu: 2.975 ± 0.08	2.378TyrPhe: 2.378 ± 0.079	2.423TyrGly: 2.423 ± 0.065	0.537TyrHis: 0.537 ± 0.031	3.283TyrIle: 3.283 ± 0.076	2.179TyrLys: 2.179 ± 0.074	3.217TyrLeu: 3.217 ± 0.082	0.569TyrMet: 0.569 ± 0.031	1.128TyrAsn: 1.128 ± 0.049	1.527TyrPro: 1.527 ± 0.05	0.832TyrGln: 0.832 ± 0.039	1.212TyrArg: 1.212 ± 0.046	2.543TyrSer: 2.543 ± 0.074	1.227TyrThr: 1.227 ± 0.044	2.167TyrVal: 2.167 ± 0.066	0.276TyrTrp: 0.276 ± 0.021	1.158TyrTyr: 1.158 ± 0.051	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 1704 proteins (576888 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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