Sulfolobus sp. SCGC AB-777_L09
Average proteome isoelectric point is 6.98
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 930 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2T9WQU8|A0A2T9WQU8_9CREN Amidohydro_3 domain-containing protein OS=Sulfolobus sp. SCGC AB-777_L09 OX=1987492 GN=DDW09_02820 PE=4 SV=1
MM1 pKa = 7.36 PPNWVFNYY9 pKa = 9.61 GALANRR15 pKa = 11.84 VTYY18 pKa = 9.98 VVNPQIPIIVTYY30 pKa = 8.14 MVPNPNGQGGPQGDD44 pKa = 3.97 VQAVIIGNQVFFEE57 pKa = 4.29 PSVNDD62 pKa = 4.55 FSSCYY67 pKa = 10.26 NGNDD71 pKa = 3.32 CSALQAEE78 pKa = 4.71 GLDD81 pKa = 4.1 LSFLGYY87 pKa = 9.87 PYY89 pKa = 10.47 FITGGGCISGSGSCPPTPNPSGVEE113 pKa = 3.94 TCNLIINKK121 pKa = 7.18 EE122 pKa = 4.26 TGEE125 pKa = 3.95 ITQLFGGALSNTLSVMADD143 pKa = 3.37 TNNGYY148 pKa = 10.48 LIIVTSTNSTSADD161 pKa = 3.98 FIIAIPFSSITSILAGQIPPDD182 pKa = 4.06 AKK184 pKa = 9.65 WVIVSTSDD192 pKa = 4.15 GSSLYY197 pKa = 10.97 ANQAEE202 pKa = 4.79 LIDD205 pKa = 3.88 GNIYY209 pKa = 10.28 FVSNTGNGVEE219 pKa = 4.29 VFKK222 pKa = 11.14 FPLSEE227 pKa = 4.34 VYY229 pKa = 10.48 NSQCTGIPQGYY240 pKa = 8.93 SSCTYY245 pKa = 8.9 TVNPLTSISQPVANSGGNILTDD267 pKa = 4.79 AIKK270 pKa = 10.45 MNGVSYY276 pKa = 10.39 LIIFYY281 pKa = 9.92 PSNGNFTVILYY292 pKa = 8.0 NTEE295 pKa = 4.12 DD296 pKa = 3.2 NTYY299 pKa = 10.61 SINTFGQNIEE309 pKa = 4.16 PQFITTGIAIYY320 pKa = 10.22 NHH322 pKa = 6.38 IVLATAQQLDD332 pKa = 4.67 GTWLLYY338 pKa = 10.63 AYY340 pKa = 10.21 DD341 pKa = 4.18 PFNGKK346 pKa = 9.18 VDD348 pKa = 3.44 STTIPNVYY356 pKa = 9.82 QAVPTDD362 pKa = 3.17 NGYY365 pKa = 10.51 VVTLSNTLSSNSQVMISIYY384 pKa = 10.35 KK385 pKa = 9.76 VLFTISPKK393 pKa = 9.82 FQNVSITKK401 pKa = 8.71 TLNQVTVQGTLIDD414 pKa = 4.16 EE415 pKa = 4.67 NSGKK419 pKa = 9.45 PLPNYY424 pKa = 9.71 PVMLVAVDD432 pKa = 3.95 GVEE435 pKa = 4.26 VNTVTDD441 pKa = 3.82 FTVLATGTTDD451 pKa = 3.16 SNGNFTLTTSDD462 pKa = 3.64 TSHH465 pKa = 6.36 SFYY468 pKa = 11.21 GVVYY472 pKa = 10.18 YY473 pKa = 9.48 PSS475 pKa = 3.18
Molecular weight: 50.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.907
IPC2_protein 3.834
IPC_protein 3.834
Toseland 3.617
ProMoST 3.999
Dawson 3.821
Bjellqvist 3.986
Wikipedia 3.77
Rodwell 3.656
Grimsley 3.528
Solomon 3.821
Lehninger 3.77
Nozaki 3.935
DTASelect 4.177
Thurlkill 3.668
EMBOSS 3.77
Sillero 3.961
Patrickios 0.006
IPC_peptide 3.808
IPC2_peptide 3.935
IPC2.peptide.svr19 3.862
Protein with the highest isoelectric point:
>tr|A0A2T9WMY4|A0A2T9WMY4_9CREN NAD-dependent dehydratase OS=Sulfolobus sp. SCGC AB-777_L09 OX=1987492 GN=DDW09_04750 PE=4 SV=1
MM1 pKa = 7.05 GRR3 pKa = 11.84 KK4 pKa = 8.53 PVFRR8 pKa = 11.84 QDD10 pKa = 3.35 VSCPSCGSHH19 pKa = 6.69 HH20 pKa = 5.61 VVKK23 pKa = 10.62 CGRR26 pKa = 11.84 PLGRR30 pKa = 11.84 QRR32 pKa = 11.84 YY33 pKa = 8.26 LCRR36 pKa = 11.84 DD37 pKa = 3.34 CGKK40 pKa = 10.41 YY41 pKa = 10.27 FLGDD45 pKa = 3.29 ASYY48 pKa = 10.17 HH49 pKa = 4.74 HH50 pKa = 7.29 HH51 pKa = 5.77 SRR53 pKa = 11.84 KK54 pKa = 9.55 LRR56 pKa = 11.84 EE57 pKa = 3.83 EE58 pKa = 4.14 ALRR61 pKa = 11.84 MYY63 pKa = 10.85 ANGMSMRR70 pKa = 11.84 AISRR74 pKa = 11.84 VLNVPLGTVFTT85 pKa = 4.86
Molecular weight: 9.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.321
IPC2_protein 9.458
IPC_protein 9.955
Toseland 10.014
ProMoST 9.838
Dawson 10.262
Bjellqvist 10.058
Wikipedia 10.482
Rodwell 10.452
Grimsley 10.35
Solomon 10.335
Lehninger 10.292
Nozaki 10.189
DTASelect 10.014
Thurlkill 10.116
EMBOSS 10.438
Sillero 10.218
Patrickios 10.028
IPC_peptide 10.321
IPC2_peptide 9.443
IPC2.peptide.svr19 8.28
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
930
0
930
236844
21
1158
254.7
28.81
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.494 ± 0.084
0.643 ± 0.023
4.693 ± 0.065
7.123 ± 0.113
4.481 ± 0.065
6.603 ± 0.077
1.343 ± 0.03
9.41 ± 0.08
7.898 ± 0.122
10.244 ± 0.089
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.19 ± 0.038
4.677 ± 0.076
4.014 ± 0.063
2.109 ± 0.047
4.286 ± 0.065
6.506 ± 0.072
4.777 ± 0.066
7.712 ± 0.066
0.991 ± 0.03
4.805 ± 0.067
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here