Quisquiliibacterium sp. CC-CFT501
Average proteome isoelectric point is 7.0
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3574 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5C8P0S8|A0A5C8P0S8_9BURK ATP-dependent DNA helicase OS=Quisquiliibacterium sp. CC-CFT501 OX=2498847 GN=FHP08_06320 PE=4 SV=1
MM1 pKa = 7.93 AIGGCGGGDD10 pKa = 3.84 DD11 pKa = 4.08 STALTVTIDD20 pKa = 3.34 GPAIVEE26 pKa = 3.98 FDD28 pKa = 3.43 GPAIVLSGTASLPEE42 pKa = 4.02 GSYY45 pKa = 8.37 VTGGTPSVPWVTCQTGPYY63 pKa = 8.57 TLTWSNAATGQSGNLTAFWNCTEE86 pKa = 4.24 SFLRR90 pKa = 11.84 WSTIPLPLALGANPVTVSMIDD111 pKa = 3.44 EE112 pKa = 4.23 RR113 pKa = 11.84 SSANDD118 pKa = 3.33 SDD120 pKa = 4.14 SVTVNRR126 pKa = 11.84 RR127 pKa = 3.0
Molecular weight: 13.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.762
IPC2_protein 3.923
IPC_protein 3.821
Toseland 3.617
ProMoST 4.012
Dawson 3.821
Bjellqvist 3.986
Wikipedia 3.783
Rodwell 3.656
Grimsley 3.541
Solomon 3.795
Lehninger 3.757
Nozaki 3.961
DTASelect 4.164
Thurlkill 3.694
EMBOSS 3.795
Sillero 3.948
Patrickios 1.914
IPC_peptide 3.795
IPC2_peptide 3.91
IPC2.peptide.svr19 3.848
Protein with the highest isoelectric point:
>tr|A0A5C8NUF0|A0A5C8NUF0_9BURK CoA-acylating methylmalonate-semialdehyde dehydrogenase OS=Quisquiliibacterium sp. CC-CFT501 OX=2498847 GN=FHP08_13765 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVTRR11 pKa = 11.84 RR12 pKa = 11.84 KK13 pKa = 8.43 RR14 pKa = 11.84 THH16 pKa = 5.88 GFLVRR21 pKa = 11.84 MKK23 pKa = 9.29 TRR25 pKa = 11.84 GGRR28 pKa = 11.84 RR29 pKa = 11.84 VLNARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.43 GRR39 pKa = 11.84 KK40 pKa = 8.97 RR41 pKa = 11.84 LGII44 pKa = 3.92
Molecular weight: 5.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.705
Grimsley 13.086
Solomon 13.539
Lehninger 13.451
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.427
IPC_peptide 13.554
IPC2_peptide 12.53
IPC2.peptide.svr19 9.21
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3574
0
3574
1175913
37
2872
329.0
35.55
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.893 ± 0.067
0.878 ± 0.011
5.36 ± 0.032
5.67 ± 0.036
3.477 ± 0.027
8.973 ± 0.041
1.997 ± 0.017
4.432 ± 0.028
2.559 ± 0.037
10.667 ± 0.052
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.353 ± 0.019
2.079 ± 0.02
5.621 ± 0.028
3.026 ± 0.024
8.374 ± 0.046
5.005 ± 0.027
4.499 ± 0.026
7.686 ± 0.034
1.486 ± 0.02
1.965 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here