Amino acid dipepetide frequency for Methylophilales bacterium MBRS-H7

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.743AlaAla: 4.743 ± 0.13
0.695AlaCys: 0.695 ± 0.037
3.522AlaAsp: 3.522 ± 0.091
3.934AlaGlu: 3.934 ± 0.117
2.818AlaPhe: 2.818 ± 0.096
4.291AlaGly: 4.291 ± 0.131
1.22AlaHis: 1.22 ± 0.05
5.534AlaIle: 5.534 ± 0.124
5.092AlaLys: 5.092 ± 0.138
6.298AlaLeu: 6.298 ± 0.138
1.736AlaMet: 1.736 ± 0.069
2.964AlaAsn: 2.964 ± 0.077
1.769AlaPro: 1.769 ± 0.065
2.168AlaGln: 2.168 ± 0.08
2.401AlaArg: 2.401 ± 0.094
4.217AlaSer: 4.217 ± 0.102
3.041AlaThr: 3.041 ± 0.089
3.912AlaVal: 3.912 ± 0.115
0.605AlaTrp: 0.605 ± 0.043
1.962AlaTyr: 1.962 ± 0.082
0.0AlaXaa: 0.0 ± 0.0
Cys
0.558CysAla: 0.558 ± 0.042
0.109CysCys: 0.109 ± 0.018
0.553CysAsp: 0.553 ± 0.038
0.595CysGlu: 0.595 ± 0.041
0.461CysPhe: 0.461 ± 0.033
0.791CysGly: 0.791 ± 0.048
0.327CysHis: 0.327 ± 0.031
0.747CysIle: 0.747 ± 0.043
0.556CysLys: 0.556 ± 0.037
0.878CysLeu: 0.878 ± 0.049
0.169CysMet: 0.169 ± 0.021
0.494CysAsn: 0.494 ± 0.039
0.496CysPro: 0.496 ± 0.033
0.29CysGln: 0.29 ± 0.031
0.283CysArg: 0.283 ± 0.027
0.662CysSer: 0.662 ± 0.045
0.389CysThr: 0.389 ± 0.029
0.494CysVal: 0.494 ± 0.037
0.072CysTrp: 0.072 ± 0.013
0.275CysTyr: 0.275 ± 0.025
0.0CysXaa: 0.0 ± 0.0
Asp
3.475AspAla: 3.475 ± 0.09
0.561AspCys: 0.561 ± 0.037
3.47AspAsp: 3.47 ± 0.09
4.189AspGlu: 4.189 ± 0.104
3.135AspPhe: 3.135 ± 0.088
3.683AspGly: 3.683 ± 0.12
1.362AspHis: 1.362 ± 0.064
5.095AspIle: 5.095 ± 0.135
4.083AspLys: 4.083 ± 0.099
6.201AspLeu: 6.201 ± 0.121
1.245AspMet: 1.245 ± 0.065
3.063AspAsn: 3.063 ± 0.095
2.262AspPro: 2.262 ± 0.081
2.639AspGln: 2.639 ± 0.078
2.185AspArg: 2.185 ± 0.086
3.416AspSer: 3.416 ± 0.095
2.272AspThr: 2.272 ± 0.078
3.671AspVal: 3.671 ± 0.1
0.657AspTrp: 0.657 ± 0.047
2.247AspTyr: 2.247 ± 0.077
0.0AspXaa: 0.0 ± 0.0
Glu
4.023GluAla: 4.023 ± 0.112
0.528GluCys: 0.528 ± 0.036
3.428GluAsp: 3.428 ± 0.094
4.122GluGlu: 4.122 ± 0.133
2.994GluPhe: 2.994 ± 0.092
3.016GluGly: 3.016 ± 0.102
1.181GluHis: 1.181 ± 0.056
6.342GluIle: 6.342 ± 0.128
5.911GluLys: 5.911 ± 0.131
6.104GluLeu: 6.104 ± 0.114
1.55GluMet: 1.55 ± 0.063
4.286GluAsn: 4.286 ± 0.094
1.585GluPro: 1.585 ± 0.066
2.183GluGln: 2.183 ± 0.075
2.297GluArg: 2.297 ± 0.091
4.085GluSer: 4.085 ± 0.114
2.741GluThr: 2.741 ± 0.088
4.036GluVal: 4.036 ± 0.114
0.57GluTrp: 0.57 ± 0.038
1.873GluTyr: 1.873 ± 0.065
0.0GluXaa: 0.0 ± 0.0
Phe
2.962PheAla: 2.962 ± 0.089
0.573PheCys: 0.573 ± 0.038
3.326PheAsp: 3.326 ± 0.109
3.083PheGlu: 3.083 ± 0.081
3.081PhePhe: 3.081 ± 0.115
3.056PheGly: 3.056 ± 0.097
1.027PheHis: 1.027 ± 0.042
4.748PheIle: 4.748 ± 0.125
3.505PheLys: 3.505 ± 0.1
4.986PheLeu: 4.986 ± 0.154
1.121PheMet: 1.121 ± 0.055
3.267PheAsn: 3.267 ± 0.109
1.647PhePro: 1.647 ± 0.068
1.454PheGln: 1.454 ± 0.064
1.53PheArg: 1.53 ± 0.066
3.686PheSer: 3.686 ± 0.096
2.225PheThr: 2.225 ± 0.072
2.828PheVal: 2.828 ± 0.105
0.506PheTrp: 0.506 ± 0.035
1.883PheTyr: 1.883 ± 0.075
0.0PheXaa: 0.0 ± 0.0
Gly
4.108GlyAla: 4.108 ± 0.122
0.742GlyCys: 0.742 ± 0.043
3.319GlyAsp: 3.319 ± 0.102
3.507GlyGlu: 3.507 ± 0.097
3.247GlyPhe: 3.247 ± 0.105
4.38GlyGly: 4.38 ± 0.137
1.481GlyHis: 1.481 ± 0.068
5.437GlyIle: 5.437 ± 0.141
4.428GlyLys: 4.428 ± 0.112
6.129GlyLeu: 6.129 ± 0.143
1.672GlyMet: 1.672 ± 0.065
2.726GlyAsn: 2.726 ± 0.085
1.662GlyPro: 1.662 ± 0.068
2.046GlyGln: 2.046 ± 0.073
2.374GlyArg: 2.374 ± 0.085
3.899GlySer: 3.899 ± 0.1
2.805GlyThr: 2.805 ± 0.09
4.122GlyVal: 4.122 ± 0.113
0.667GlyTrp: 0.667 ± 0.044
2.376GlyTyr: 2.376 ± 0.079
0.0GlyXaa: 0.0 ± 0.0
His
1.399HisAla: 1.399 ± 0.06
0.285HisCys: 0.285 ± 0.025
1.208HisAsp: 1.208 ± 0.058
1.235HisGlu: 1.235 ± 0.054
1.148HisPhe: 1.148 ± 0.053
1.444HisGly: 1.444 ± 0.064
0.655HisHis: 0.655 ± 0.047
1.573HisIle: 1.573 ± 0.063
1.255HisLys: 1.255 ± 0.06
2.131HisLeu: 2.131 ± 0.081
0.449HisMet: 0.449 ± 0.034
1.022HisAsn: 1.022 ± 0.05
1.057HisPro: 1.057 ± 0.047
0.908HisGln: 0.908 ± 0.052
0.774HisArg: 0.774 ± 0.048
1.31HisSer: 1.31 ± 0.066
0.895HisThr: 0.895 ± 0.049
1.136HisVal: 1.136 ± 0.061
0.226HisTrp: 0.226 ± 0.024
0.734HisTyr: 0.734 ± 0.034
0.0HisXaa: 0.0 ± 0.0
Ile
5.849IleAla: 5.849 ± 0.125
0.754IleCys: 0.754 ± 0.041
6.186IleAsp: 6.186 ± 0.124
5.995IleGlu: 5.995 ± 0.118
4.549IlePhe: 4.549 ± 0.138
5.345IleGly: 5.345 ± 0.129
1.843IleHis: 1.843 ± 0.07
7.193IleIle: 7.193 ± 0.145
7.607IleLys: 7.607 ± 0.129
7.789IleLeu: 7.789 ± 0.171
1.746IleMet: 1.746 ± 0.069
6.075IleAsn: 6.075 ± 0.145
3.562IlePro: 3.562 ± 0.099
3.088IleGln: 3.088 ± 0.09
2.947IleArg: 2.947 ± 0.096
6.422IleSer: 6.422 ± 0.146
4.566IleThr: 4.566 ± 0.108
4.765IleVal: 4.765 ± 0.117
0.667IleTrp: 0.667 ± 0.041
2.726IleTyr: 2.726 ± 0.092
0.0IleXaa: 0.0 ± 0.0
Lys
4.214LysAla: 4.214 ± 0.112
0.454LysCys: 0.454 ± 0.034
4.693LysAsp: 4.693 ± 0.121
5.38LysGlu: 5.38 ± 0.128
3.344LysPhe: 3.344 ± 0.103
3.671LysGly: 3.671 ± 0.105
1.394LysHis: 1.394 ± 0.064
8.123LysIle: 8.123 ± 0.179
8.892LysLys: 8.892 ± 0.205
7.228LysLeu: 7.228 ± 0.131
1.89LysMet: 1.89 ± 0.068
6.461LysAsn: 6.461 ± 0.15
2.537LysPro: 2.537 ± 0.086
2.766LysGln: 2.766 ± 0.084
2.776LysArg: 2.776 ± 0.076
4.981LysSer: 4.981 ± 0.11
4.115LysThr: 4.115 ± 0.102
4.361LysVal: 4.361 ± 0.116
0.575LysTrp: 0.575 ± 0.041
2.614LysTyr: 2.614 ± 0.087
0.0LysXaa: 0.0 ± 0.0
Leu
6.308LeuAla: 6.308 ± 0.133
0.779LeuCys: 0.779 ± 0.048
5.484LeuAsp: 5.484 ± 0.137
5.447LeuGlu: 5.447 ± 0.119
4.77LeuPhe: 4.77 ± 0.135
6.094LeuGly: 6.094 ± 0.132
1.689LeuHis: 1.689 ± 0.062
9.237LeuIle: 9.237 ± 0.183
8.237LeuLys: 8.237 ± 0.141
8.761LeuLeu: 8.761 ± 0.186
2.478LeuMet: 2.478 ± 0.076
6.377LeuAsn: 6.377 ± 0.139
3.564LeuPro: 3.564 ± 0.101
2.954LeuGln: 2.954 ± 0.09
3.49LeuArg: 3.49 ± 0.096
7.471LeuSer: 7.471 ± 0.15
5.045LeuThr: 5.045 ± 0.118
5.472LeuVal: 5.472 ± 0.122
0.836LeuTrp: 0.836 ± 0.05
2.488LeuTyr: 2.488 ± 0.087
0.0LeuXaa: 0.0 ± 0.0
Met
1.776MetAla: 1.776 ± 0.065
0.164MetCys: 0.164 ± 0.021
1.33MetAsp: 1.33 ± 0.066
1.297MetGlu: 1.297 ± 0.064
0.92MetPhe: 0.92 ± 0.047
1.523MetGly: 1.523 ± 0.073
0.511MetHis: 0.511 ± 0.031
1.94MetIle: 1.94 ± 0.07
1.89MetLys: 1.89 ± 0.069
2.141MetLeu: 2.141 ± 0.084
0.717MetMet: 0.717 ± 0.042
1.456MetAsn: 1.456 ± 0.069
0.987MetPro: 0.987 ± 0.051
1.062MetGln: 1.062 ± 0.051
0.952MetArg: 0.952 ± 0.049
1.751MetSer: 1.751 ± 0.066
1.392MetThr: 1.392 ± 0.059
1.449MetVal: 1.449 ± 0.057
0.166MetTrp: 0.166 ± 0.02
0.499MetTyr: 0.499 ± 0.037
0.0MetXaa: 0.0 ± 0.0
Asn
3.115AsnAla: 3.115 ± 0.089
0.499AsnCys: 0.499 ± 0.037
3.487AsnAsp: 3.487 ± 0.093
3.85AsnGlu: 3.85 ± 0.104
3.336AsnPhe: 3.336 ± 0.105
2.959AsnGly: 2.959 ± 0.097
1.359AsnHis: 1.359 ± 0.059
5.618AsnIle: 5.618 ± 0.135
5.241AsnLys: 5.241 ± 0.147
6.156AsnLeu: 6.156 ± 0.147
1.186AsnMet: 1.186 ± 0.057
3.877AsnAsn: 3.877 ± 0.122
2.47AsnPro: 2.47 ± 0.078
2.986AsnGln: 2.986 ± 0.087
1.895AsnArg: 1.895 ± 0.063
3.497AsnSer: 3.497 ± 0.099
2.371AsnThr: 2.371 ± 0.074
3.063AsnVal: 3.063 ± 0.094
0.593AsnTrp: 0.593 ± 0.04
2.217AsnTyr: 2.217 ± 0.081
0.0AsnXaa: 0.0 ± 0.0
Pro
2.074ProAla: 2.074 ± 0.083
0.273ProCys: 0.273 ± 0.026
2.146ProAsp: 2.146 ± 0.079
2.562ProGlu: 2.562 ± 0.076
1.786ProPhe: 1.786 ± 0.066
2.376ProGly: 2.376 ± 0.089
0.719ProHis: 0.719 ± 0.037
2.897ProIle: 2.897 ± 0.074
2.676ProLys: 2.676 ± 0.082
3.306ProLeu: 3.306 ± 0.091
0.856ProMet: 0.856 ± 0.045
2.019ProAsn: 2.019 ± 0.078
1.002ProPro: 1.002 ± 0.055
1.163ProGln: 1.163 ± 0.053
1.042ProArg: 1.042 ± 0.054
2.334ProSer: 2.334 ± 0.071
1.855ProThr: 1.855 ± 0.072
2.346ProVal: 2.346 ± 0.081
0.342ProTrp: 0.342 ± 0.035
1.245ProTyr: 1.245 ± 0.054
0.0ProXaa: 0.0 ± 0.0
Gln
2.324GlnAla: 2.324 ± 0.073
0.322GlnCys: 0.322 ± 0.03
2.076GlnAsp: 2.076 ± 0.063
2.262GlnGlu: 2.262 ± 0.083
1.694GlnPhe: 1.694 ± 0.065
1.91GlnGly: 1.91 ± 0.073
0.781GlnHis: 0.781 ± 0.05
3.282GlnIle: 3.282 ± 0.096
3.128GlnLys: 3.128 ± 0.084
3.683GlnLeu: 3.683 ± 0.1
0.866GlnMet: 0.866 ± 0.042
2.029GlnAsn: 2.029 ± 0.075
1.233GlnPro: 1.233 ± 0.054
1.436GlnGln: 1.436 ± 0.063
1.406GlnArg: 1.406 ± 0.065
2.595GlnSer: 2.595 ± 0.081
1.625GlnThr: 1.625 ± 0.075
2.093GlnVal: 2.093 ± 0.083
0.34GlnTrp: 0.34 ± 0.027
1.176GlnTyr: 1.176 ± 0.056
0.0GlnXaa: 0.0 ± 0.0
Arg
2.255ArgAla: 2.255 ± 0.072
0.332ArgCys: 0.332 ± 0.028
1.994ArgAsp: 1.994 ± 0.076
2.401ArgGlu: 2.401 ± 0.082
1.922ArgPhe: 1.922 ± 0.066
2.151ArgGly: 2.151 ± 0.071
0.771ArgHis: 0.771 ± 0.041
2.996ArgIle: 2.996 ± 0.09
2.545ArgLys: 2.545 ± 0.084
3.502ArgLeu: 3.502 ± 0.094
1.019ArgMet: 1.019 ± 0.053
1.92ArgAsn: 1.92 ± 0.068
1.255ArgPro: 1.255 ± 0.056
1.32ArgGln: 1.32 ± 0.055
1.518ArgArg: 1.518 ± 0.066
2.384ArgSer: 2.384 ± 0.074
1.617ArgThr: 1.617 ± 0.059
2.314ArgVal: 2.314 ± 0.079
0.273ArgTrp: 0.273 ± 0.027
1.359ArgTyr: 1.359 ± 0.065
0.0ArgXaa: 0.0 ± 0.0
Ser
3.936SerAla: 3.936 ± 0.098
0.645SerCys: 0.645 ± 0.041
3.922SerAsp: 3.922 ± 0.092
4.06SerGlu: 4.06 ± 0.112
3.649SerPhe: 3.649 ± 0.1
4.519SerGly: 4.519 ± 0.114
1.394SerHis: 1.394 ± 0.059
6.308SerIle: 6.308 ± 0.132
5.015SerLys: 5.015 ± 0.118
6.955SerLeu: 6.955 ± 0.143
1.751SerMet: 1.751 ± 0.077
3.946SerAsn: 3.946 ± 0.103
2.289SerPro: 2.289 ± 0.072
2.448SerGln: 2.448 ± 0.073
2.468SerArg: 2.468 ± 0.077
5.112SerSer: 5.112 ± 0.131
2.932SerThr: 2.932 ± 0.095
3.939SerVal: 3.939 ± 0.112
0.692SerTrp: 0.692 ± 0.043
2.101SerTyr: 2.101 ± 0.075
0.0SerXaa: 0.0 ± 0.0
Thr
2.897ThrAla: 2.897 ± 0.098
0.437ThrCys: 0.437 ± 0.034
2.694ThrAsp: 2.694 ± 0.081
2.756ThrGlu: 2.756 ± 0.081
2.356ThrPhe: 2.356 ± 0.082
3.42ThrGly: 3.42 ± 0.114
1.057ThrHis: 1.057 ± 0.05
4.036ThrIle: 4.036 ± 0.102
3.525ThrLys: 3.525 ± 0.109
4.78ThrLeu: 4.78 ± 0.121
0.97ThrMet: 0.97 ± 0.046
2.647ThrAsn: 2.647 ± 0.084
2.093ThrPro: 2.093 ± 0.08
1.684ThrGln: 1.684 ± 0.065
1.545ThrArg: 1.545 ± 0.065
3.15ThrSer: 3.15 ± 0.093
2.304ThrThr: 2.304 ± 0.089
2.805ThrVal: 2.805 ± 0.094
0.375ThrTrp: 0.375 ± 0.029
1.555ThrTyr: 1.555 ± 0.063
0.0ThrXaa: 0.0 ± 0.0
Val
4.209ValAla: 4.209 ± 0.124
0.64ValCys: 0.64 ± 0.041
3.634ValAsp: 3.634 ± 0.104
3.599ValGlu: 3.599 ± 0.105
2.813ValPhe: 2.813 ± 0.1
3.974ValGly: 3.974 ± 0.102
1.081ValHis: 1.081 ± 0.052
5.509ValIle: 5.509 ± 0.117
4.117ValLys: 4.117 ± 0.136
5.333ValLeu: 5.333 ± 0.109
1.677ValMet: 1.677 ± 0.062
3.274ValAsn: 3.274 ± 0.088
2.012ValPro: 2.012 ± 0.074
1.667ValGln: 1.667 ± 0.064
2.131ValArg: 2.131 ± 0.075
4.244ValSer: 4.244 ± 0.117
3.244ValThr: 3.244 ± 0.096
4.093ValVal: 4.093 ± 0.128
0.479ValTrp: 0.479 ± 0.04
1.53ValTyr: 1.53 ± 0.065
0.0ValXaa: 0.0 ± 0.0
Trp
0.541TrpAla: 0.541 ± 0.037
0.094TrpCys: 0.094 ± 0.016
0.429TrpAsp: 0.429 ± 0.035
0.489TrpGlu: 0.489 ± 0.034
0.563TrpPhe: 0.563 ± 0.04
0.538TrpGly: 0.538 ± 0.039
0.231TrpHis: 0.231 ± 0.022
0.769TrpIle: 0.769 ± 0.046
0.687TrpLys: 0.687 ± 0.039
1.094TrpLeu: 1.094 ± 0.055
0.248TrpMet: 0.248 ± 0.028
0.464TrpAsn: 0.464 ± 0.034
0.258TrpPro: 0.258 ± 0.029
0.375TrpGln: 0.375 ± 0.031
0.377TrpArg: 0.377 ± 0.028
0.61TrpSer: 0.61 ± 0.043
0.362TrpThr: 0.362 ± 0.037
0.588TrpVal: 0.588 ± 0.04
0.112TrpTrp: 0.112 ± 0.017
0.283TrpTyr: 0.283 ± 0.026
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.155TyrAla: 2.155 ± 0.077
0.315TyrCys: 0.315 ± 0.032
2.012TyrAsp: 2.012 ± 0.072
2.091TyrGlu: 2.091 ± 0.069
1.905TyrPhe: 1.905 ± 0.072
1.984TyrGly: 1.984 ± 0.073
0.759TyrHis: 0.759 ± 0.047
2.292TyrIle: 2.292 ± 0.078
2.079TyrLys: 2.079 ± 0.09
3.527TyrLeu: 3.527 ± 0.114
0.575TyrMet: 0.575 ± 0.037
1.411TyrAsn: 1.411 ± 0.063
1.28TyrPro: 1.28 ± 0.059
1.625TyrGln: 1.625 ± 0.065
1.458TyrArg: 1.458 ± 0.061
2.257TyrSer: 2.257 ± 0.088
1.287TyrThr: 1.287 ± 0.051
1.773TyrVal: 1.773 ± 0.065
0.357TyrTrp: 0.357 ± 0.027
1.01TyrTyr: 1.01 ± 0.054
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1317 proteins (403159 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski