Erysipelothrix sp. HDW6C

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Erysipelotrichia; Erysipelotrichales; Erysipelotrichaceae; Erysipelothrix; unclassified Erysipelothrix

Average proteome isoelectric point is 5.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2192 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G7Y281|A0A6G7Y281_9FIRM Uncharacterized protein OS=Erysipelothrix sp. HDW6C OX=2714930 GN=G7062_10575 PE=4 SV=1
MM1 pKa = 6.6EE2 pKa = 5.04TGRR5 pKa = 11.84GWVAEE10 pKa = 4.11EE11 pKa = 4.73AIAIYY16 pKa = 10.32VALRR20 pKa = 11.84YY21 pKa = 9.56QDD23 pKa = 5.39DD24 pKa = 4.17IISAYY29 pKa = 9.61EE30 pKa = 3.45VAIYY34 pKa = 10.29HH35 pKa = 7.23DD36 pKa = 4.74EE37 pKa = 5.17DD38 pKa = 5.45GDD40 pKa = 4.24STGSILGNILGTYY53 pKa = 10.39LSFEE57 pKa = 4.65ALPKK61 pKa = 11.07AMVAALDD68 pKa = 3.59IGDD71 pKa = 3.49ITEE74 pKa = 4.31RR75 pKa = 11.84LINDD79 pKa = 3.66APP81 pKa = 3.71

Molecular weight: 8.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G7XWV5|A0A6G7XWV5_9FIRM Thymidylate kinase OS=Erysipelothrix sp. HDW6C OX=2714930 GN=tmk PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.14QPSKK9 pKa = 9.95RR10 pKa = 11.84KK11 pKa = 7.81HH12 pKa = 5.11QKK14 pKa = 8.48VHH16 pKa = 5.53GFRR19 pKa = 11.84ARR21 pKa = 11.84MKK23 pKa = 8.28TVGGRR28 pKa = 11.84RR29 pKa = 11.84VLSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84SKK37 pKa = 10.63GRR39 pKa = 11.84KK40 pKa = 8.44VLSAA44 pKa = 4.05

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2192

0

2192

705938

31

5162

322.1

36.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.04 ± 0.054

0.628 ± 0.015

6.139 ± 0.046

6.537 ± 0.05

4.476 ± 0.045

6.439 ± 0.046

2.044 ± 0.025

8.262 ± 0.052

5.876 ± 0.041

9.173 ± 0.065

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.895 ± 0.035

5.077 ± 0.053

3.181 ± 0.033

3.425 ± 0.027

4.052 ± 0.046

6.296 ± 0.043

6.214 ± 0.074

7.608 ± 0.052

0.743 ± 0.016

3.894 ± 0.046
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski