Spiroplasma apis B31

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Entomoplasmatales; Spiroplasmataceae; Spiroplasma; Spiroplasma apis

Average proteome isoelectric point is 7.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 992 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|V5RI97|V5RI97_SPIAP Uncharacterized protein OS=Spiroplasma apis B31 OX=1276258 GN=SAPIS_v1c03420 PE=4 SV=1
MM1 pKa = 7.66KK2 pKa = 9.14KK3 pKa = 6.94TTFNEE8 pKa = 3.24EE9 pKa = 3.98DD10 pKa = 3.95FIKK13 pKa = 10.82FHH15 pKa = 5.77DD16 pKa = 4.09TKK18 pKa = 11.19NIMMQLFGITCSVCGIDD35 pKa = 3.66EE36 pKa = 4.23IGYY39 pKa = 9.12VASNSPKK46 pKa = 10.62SLGDD50 pKa = 3.5LAFDD54 pKa = 3.76ALEE57 pKa = 4.4NNPSISDD64 pKa = 3.74EE65 pKa = 4.15EE66 pKa = 4.36LDD68 pKa = 5.25KK69 pKa = 11.74LMEE72 pKa = 4.83DD73 pKa = 5.2PIEE76 pKa = 4.21AWQEE80 pKa = 3.67VDD82 pKa = 5.35DD83 pKa = 4.33YY84 pKa = 10.88NASIGMPTFACDD96 pKa = 2.76NCYY99 pKa = 10.0QQLIDD104 pKa = 4.75GEE106 pKa = 4.38IQISSIEE113 pKa = 4.04EE114 pKa = 3.76EE115 pKa = 4.19

Molecular weight: 12.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|V5RL43|V5RL43_SPIAP Sucrose-6-phosphate hydrolase OS=Spiroplasma apis B31 OX=1276258 GN=scrB2 PE=3 SV=1
MM1 pKa = 7.32KK2 pKa = 9.47RR3 pKa = 11.84TWQPSKK9 pKa = 10.03IKK11 pKa = 10.14HH12 pKa = 5.53ARR14 pKa = 11.84THH16 pKa = 5.78GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.41NGRR28 pKa = 11.84KK29 pKa = 9.2VIKK32 pKa = 10.14ARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.28GRR39 pKa = 11.84ARR41 pKa = 11.84LSAA44 pKa = 3.91

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

992

0

992

338527

29

1480

341.3

39.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.738 ± 0.067

0.77 ± 0.018

5.391 ± 0.061

6.829 ± 0.077

5.471 ± 0.072

5.104 ± 0.073

1.258 ± 0.029

9.779 ± 0.07

10.228 ± 0.082

9.663 ± 0.081

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.18 ± 0.026

7.545 ± 0.08

2.499 ± 0.037

2.722 ± 0.037

2.849 ± 0.049

6.781 ± 0.067

5.185 ± 0.047

5.847 ± 0.051

0.997 ± 0.026

4.165 ± 0.051
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski