Amino acid dipepetide frequency for Spiroplasma apis B31

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.83AlaAla: 2.83 ± 0.114
0.479AlaCys: 0.479 ± 0.039
2.047AlaAsp: 2.047 ± 0.086
2.402AlaGlu: 2.402 ± 0.099
2.623AlaPhe: 2.623 ± 0.1
2.839AlaGly: 2.839 ± 0.11
0.653AlaHis: 0.653 ± 0.047
4.983AlaIle: 4.983 ± 0.147
4.709AlaLys: 4.709 ± 0.122
4.697AlaLeu: 4.697 ± 0.155
1.146AlaMet: 1.146 ± 0.057
3.06AlaAsn: 3.06 ± 0.094
1.185AlaPro: 1.185 ± 0.063
1.264AlaGln: 1.264 ± 0.056
1.554AlaArg: 1.554 ± 0.069
3.267AlaSer: 3.267 ± 0.098
2.735AlaThr: 2.735 ± 0.1
2.611AlaVal: 2.611 ± 0.094
0.437AlaTrp: 0.437 ± 0.037
1.861AlaTyr: 1.861 ± 0.08
0.0AlaXaa: 0.0 ± 0.0
Cys
0.375CysAla: 0.375 ± 0.037
0.127CysCys: 0.127 ± 0.021
0.452CysAsp: 0.452 ± 0.033
0.579CysGlu: 0.579 ± 0.041
0.481CysPhe: 0.481 ± 0.038
0.535CysGly: 0.535 ± 0.041
0.121CysHis: 0.121 ± 0.018
0.685CysIle: 0.685 ± 0.045
0.597CysLys: 0.597 ± 0.048
0.824CysLeu: 0.824 ± 0.055
0.127CysMet: 0.127 ± 0.019
0.458CysAsn: 0.458 ± 0.039
0.189CysPro: 0.189 ± 0.024
0.207CysGln: 0.207 ± 0.023
0.157CysArg: 0.157 ± 0.021
0.611CysSer: 0.611 ± 0.045
0.304CysThr: 0.304 ± 0.03
0.431CysVal: 0.431 ± 0.035
0.092CysTrp: 0.092 ± 0.017
0.343CysTyr: 0.343 ± 0.037
0.0CysXaa: 0.0 ± 0.0
Asp
2.207AspAla: 2.207 ± 0.081
0.366AspCys: 0.366 ± 0.036
2.986AspAsp: 2.986 ± 0.105
3.997AspGlu: 3.997 ± 0.123
3.58AspPhe: 3.58 ± 0.109
2.6AspGly: 2.6 ± 0.099
0.671AspHis: 0.671 ± 0.043
5.471AspIle: 5.471 ± 0.137
5.424AspLys: 5.424 ± 0.157
5.613AspLeu: 5.613 ± 0.148
1.114AspMet: 1.114 ± 0.058
3.743AspAsn: 3.743 ± 0.11
1.332AspPro: 1.332 ± 0.07
1.38AspGln: 1.38 ± 0.059
1.235AspArg: 1.235 ± 0.05
3.238AspSer: 3.238 ± 0.11
2.381AspThr: 2.381 ± 0.091
3.111AspVal: 3.111 ± 0.101
0.62AspTrp: 0.62 ± 0.045
2.842AspTyr: 2.842 ± 0.102
0.0AspXaa: 0.0 ± 0.0
Glu
3.282GluAla: 3.282 ± 0.11
0.378GluCys: 0.378 ± 0.036
3.282GluAsp: 3.282 ± 0.112
5.196GluGlu: 5.196 ± 0.176
3.332GluPhe: 3.332 ± 0.102
2.765GluGly: 2.765 ± 0.094
0.892GluHis: 0.892 ± 0.048
6.838GluIle: 6.838 ± 0.187
7.928GluLys: 7.928 ± 0.209
6.655GluLeu: 6.655 ± 0.182
1.498GluMet: 1.498 ± 0.071
5.861GluAsn: 5.861 ± 0.138
1.403GluPro: 1.403 ± 0.068
2.041GluGln: 2.041 ± 0.072
2.097GluArg: 2.097 ± 0.1
3.961GluSer: 3.961 ± 0.123
3.211GluThr: 3.211 ± 0.102
4.481GluVal: 4.481 ± 0.14
0.638GluTrp: 0.638 ± 0.044
2.546GluTyr: 2.546 ± 0.085
0.0GluXaa: 0.0 ± 0.0
Phe
2.653PheAla: 2.653 ± 0.105
0.443PheCys: 0.443 ± 0.038
3.424PheAsp: 3.424 ± 0.105
3.796PheGlu: 3.796 ± 0.109
2.978PhePhe: 2.978 ± 0.115
3.096PheGly: 3.096 ± 0.105
0.523PheHis: 0.523 ± 0.038
5.497PheIle: 5.497 ± 0.177
5.305PheLys: 5.305 ± 0.132
5.32PheLeu: 5.32 ± 0.136
1.196PheMet: 1.196 ± 0.062
3.817PheAsn: 3.817 ± 0.126
1.075PhePro: 1.075 ± 0.055
1.27PheGln: 1.27 ± 0.065
1.38PheArg: 1.38 ± 0.057
3.967PheSer: 3.967 ± 0.109
2.898PheThr: 2.898 ± 0.105
3.397PheVal: 3.397 ± 0.101
0.499PheTrp: 0.499 ± 0.043
2.174PheTyr: 2.174 ± 0.09
0.0PheXaa: 0.0 ± 0.0
Gly
2.93GlyAla: 2.93 ± 0.12
0.402GlyCys: 0.402 ± 0.036
2.484GlyAsp: 2.484 ± 0.095
2.954GlyGlu: 2.954 ± 0.087
2.916GlyPhe: 2.916 ± 0.096
3.14GlyGly: 3.14 ± 0.132
0.815GlyHis: 0.815 ± 0.058
4.954GlyIle: 4.954 ± 0.132
4.458GlyLys: 4.458 ± 0.117
4.91GlyLeu: 4.91 ± 0.135
1.261GlyMet: 1.261 ± 0.067
2.7GlyAsn: 2.7 ± 0.094
1.108GlyPro: 1.108 ± 0.065
1.403GlyGln: 1.403 ± 0.067
1.527GlyArg: 1.527 ± 0.078
3.35GlySer: 3.35 ± 0.101
2.913GlyThr: 2.913 ± 0.091
3.796GlyVal: 3.796 ± 0.125
0.682GlyTrp: 0.682 ± 0.05
2.34GlyTyr: 2.34 ± 0.085
0.0GlyXaa: 0.0 ± 0.0
His
0.549HisAla: 0.549 ± 0.04
0.086HisCys: 0.086 ± 0.016
0.611HisAsp: 0.611 ± 0.042
0.789HisGlu: 0.789 ± 0.047
0.783HisPhe: 0.783 ± 0.055
0.762HisGly: 0.762 ± 0.052
0.239HisHis: 0.239 ± 0.025
1.255HisIle: 1.255 ± 0.063
1.229HisLys: 1.229 ± 0.064
1.199HisLeu: 1.199 ± 0.061
0.207HisMet: 0.207 ± 0.029
0.892HisAsn: 0.892 ± 0.055
0.455HisPro: 0.455 ± 0.035
0.372HisGln: 0.372 ± 0.037
0.434HisArg: 0.434 ± 0.036
0.907HisSer: 0.907 ± 0.052
0.564HisThr: 0.564 ± 0.043
0.594HisVal: 0.594 ± 0.045
0.124HisTrp: 0.124 ± 0.016
0.526HisTyr: 0.526 ± 0.037
0.0HisXaa: 0.0 ± 0.0
Ile
4.945IleAla: 4.945 ± 0.152
0.954IleCys: 0.954 ± 0.049
5.831IleAsp: 5.831 ± 0.12
6.691IleGlu: 6.691 ± 0.164
5.282IlePhe: 5.282 ± 0.153
4.963IleGly: 4.963 ± 0.127
1.013IleHis: 1.013 ± 0.057
9.535IleIle: 9.535 ± 0.202
9.878IleLys: 9.878 ± 0.184
8.912IleLeu: 8.912 ± 0.196
2.115IleMet: 2.115 ± 0.077
7.45IleAsn: 7.45 ± 0.162
2.579IlePro: 2.579 ± 0.107
2.39IleGln: 2.39 ± 0.095
2.6IleArg: 2.6 ± 0.087
7.075IleSer: 7.075 ± 0.142
5.143IleThr: 5.143 ± 0.115
5.633IleVal: 5.633 ± 0.132
0.744IleTrp: 0.744 ± 0.039
4.05IleTyr: 4.05 ± 0.118
0.0IleXaa: 0.0 ± 0.0
Lys
4.499LysAla: 4.499 ± 0.133
0.493LysCys: 0.493 ± 0.041
6.002LysAsp: 6.002 ± 0.149
8.756LysGlu: 8.756 ± 0.174
4.139LysPhe: 4.139 ± 0.102
3.997LysGly: 3.997 ± 0.092
1.326LysHis: 1.326 ± 0.058
9.349LysIle: 9.349 ± 0.171
11.154LysLys: 11.154 ± 0.221
9.166LysLeu: 9.166 ± 0.181
2.475LysMet: 2.475 ± 0.087
9.264LysAsn: 9.264 ± 0.188
2.419LysPro: 2.419 ± 0.082
3.031LysGln: 3.031 ± 0.101
3.134LysArg: 3.134 ± 0.1
5.929LysSer: 5.929 ± 0.143
5.846LysThr: 5.846 ± 0.122
5.681LysVal: 5.681 ± 0.133
0.951LysTrp: 0.951 ± 0.047
4.673LysTyr: 4.673 ± 0.132
0.0LysXaa: 0.0 ± 0.0
Leu
4.856LeuAla: 4.856 ± 0.139
0.727LeuCys: 0.727 ± 0.053
5.232LeuAsp: 5.232 ± 0.138
6.638LeuGlu: 6.638 ± 0.176
5.409LeuPhe: 5.409 ± 0.151
5.078LeuGly: 5.078 ± 0.145
1.093LeuHis: 1.093 ± 0.055
9.027LeuIle: 9.027 ± 0.178
10.055LeuLys: 10.055 ± 0.195
8.992LeuLeu: 8.992 ± 0.189
2.035LeuMet: 2.035 ± 0.076
7.391LeuAsn: 7.391 ± 0.158
2.579LeuPro: 2.579 ± 0.091
2.428LeuGln: 2.428 ± 0.085
2.735LeuArg: 2.735 ± 0.094
7.32LeuSer: 7.32 ± 0.183
5.282LeuThr: 5.282 ± 0.108
5.802LeuVal: 5.802 ± 0.137
0.904LeuTrp: 0.904 ± 0.054
3.051LeuTyr: 3.051 ± 0.09
0.0LeuXaa: 0.0 ± 0.0
Met
1.309MetAla: 1.309 ± 0.068
0.192MetCys: 0.192 ± 0.027
1.031MetAsp: 1.031 ± 0.061
1.335MetGlu: 1.335 ± 0.075
1.108MetPhe: 1.108 ± 0.058
1.306MetGly: 1.306 ± 0.068
0.284MetHis: 0.284 ± 0.028
2.133MetIle: 2.133 ± 0.073
2.481MetLys: 2.481 ± 0.076
1.899MetLeu: 1.899 ± 0.073
0.434MetMet: 0.434 ± 0.043
1.66MetAsn: 1.66 ± 0.067
0.697MetPro: 0.697 ± 0.047
0.62MetGln: 0.62 ± 0.043
0.614MetArg: 0.614 ± 0.044
1.56MetSer: 1.56 ± 0.066
0.99MetThr: 0.99 ± 0.05
1.193MetVal: 1.193 ± 0.058
0.157MetTrp: 0.157 ± 0.022
0.798MetTyr: 0.798 ± 0.052
0.0MetXaa: 0.0 ± 0.0
Asn
2.806AsnAla: 2.806 ± 0.107
0.573AsnCys: 0.573 ± 0.04
4.292AsnAsp: 4.292 ± 0.128
5.329AsnGlu: 5.329 ± 0.144
4.053AsnPhe: 4.053 ± 0.14
3.492AsnGly: 3.492 ± 0.105
0.998AsnHis: 0.998 ± 0.062
7.828AsnIle: 7.828 ± 0.151
8.519AsnLys: 8.519 ± 0.193
7.329AsnLeu: 7.329 ± 0.183
1.415AsnMet: 1.415 ± 0.06
6.212AsnAsn: 6.212 ± 0.194
2.174AsnPro: 2.174 ± 0.09
2.298AsnGln: 2.298 ± 0.082
1.699AsnArg: 1.699 ± 0.07
4.753AsnSer: 4.753 ± 0.14
3.211AsnThr: 3.211 ± 0.097
3.831AsnVal: 3.831 ± 0.1
0.836AsnTrp: 0.836 ± 0.059
3.802AsnTyr: 3.802 ± 0.13
0.0AsnXaa: 0.0 ± 0.0
Pro
0.957ProAla: 0.957 ± 0.053
0.148ProCys: 0.148 ± 0.022
1.176ProAsp: 1.176 ± 0.061
1.707ProGlu: 1.707 ± 0.08
1.492ProPhe: 1.492 ± 0.071
1.418ProGly: 1.418 ± 0.064
0.354ProHis: 0.354 ± 0.034
2.623ProIle: 2.623 ± 0.089
2.148ProLys: 2.148 ± 0.088
2.422ProLeu: 2.422 ± 0.095
0.561ProMet: 0.561 ± 0.036
1.87ProAsn: 1.87 ± 0.073
0.541ProPro: 0.541 ± 0.045
0.777ProGln: 0.777 ± 0.056
0.653ProArg: 0.653 ± 0.046
1.713ProSer: 1.713 ± 0.077
1.61ProThr: 1.61 ± 0.062
1.566ProVal: 1.566 ± 0.068
0.278ProTrp: 0.278 ± 0.033
0.978ProTyr: 0.978 ± 0.047
0.0ProXaa: 0.0 ± 0.0
Gln
1.32GlnAla: 1.32 ± 0.063
0.112GlnCys: 0.112 ± 0.019
1.344GlnAsp: 1.344 ± 0.068
2.015GlnGlu: 2.015 ± 0.09
1.226GlnPhe: 1.226 ± 0.063
1.196GlnGly: 1.196 ± 0.054
0.322GlnHis: 0.322 ± 0.03
2.608GlnIle: 2.608 ± 0.099
3.128GlnLys: 3.128 ± 0.101
2.549GlnLeu: 2.549 ± 0.085
0.614GlnMet: 0.614 ± 0.044
2.251GlnAsn: 2.251 ± 0.082
0.688GlnPro: 0.688 ± 0.05
0.933GlnGln: 0.933 ± 0.057
0.954GlnArg: 0.954 ± 0.055
1.619GlnSer: 1.619 ± 0.066
1.415GlnThr: 1.415 ± 0.061
1.622GlnVal: 1.622 ± 0.066
0.301GlnTrp: 0.301 ± 0.031
1.001GlnTyr: 1.001 ± 0.057
0.0GlnXaa: 0.0 ± 0.0
Arg
1.347ArgAla: 1.347 ± 0.058
0.227ArgCys: 0.227 ± 0.027
1.554ArgAsp: 1.554 ± 0.07
2.115ArgGlu: 2.115 ± 0.091
1.43ArgPhe: 1.43 ± 0.056
1.418ArgGly: 1.418 ± 0.071
0.408ArgHis: 0.408 ± 0.036
2.573ArgIle: 2.573 ± 0.102
2.933ArgLys: 2.933 ± 0.104
2.381ArgLeu: 2.381 ± 0.11
0.786ArgMet: 0.786 ± 0.056
2.174ArgAsn: 2.174 ± 0.09
0.744ArgPro: 0.744 ± 0.049
0.898ArgGln: 0.898 ± 0.056
1.096ArgArg: 1.096 ± 0.07
1.672ArgSer: 1.672 ± 0.07
1.394ArgThr: 1.394 ± 0.064
1.826ArgVal: 1.826 ± 0.077
0.295ArgTrp: 0.295 ± 0.031
1.223ArgTyr: 1.223 ± 0.058
0.0ArgXaa: 0.0 ± 0.0
Ser
2.771SerAla: 2.771 ± 0.08
0.603SerCys: 0.603 ± 0.043
3.134SerAsp: 3.134 ± 0.1
3.858SerGlu: 3.858 ± 0.122
4.431SerPhe: 4.431 ± 0.127
3.601SerGly: 3.601 ± 0.096
0.833SerHis: 0.833 ± 0.05
6.753SerIle: 6.753 ± 0.166
6.771SerLys: 6.771 ± 0.136
7.388SerLeu: 7.388 ± 0.148
1.359SerMet: 1.359 ± 0.069
5.019SerAsn: 5.019 ± 0.155
1.447SerPro: 1.447 ± 0.072
1.896SerGln: 1.896 ± 0.074
2.003SerArg: 2.003 ± 0.081
5.164SerSer: 5.164 ± 0.155
3.477SerThr: 3.477 ± 0.101
3.648SerVal: 3.648 ± 0.106
0.759SerTrp: 0.759 ± 0.051
2.895SerTyr: 2.895 ± 0.099
0.0SerXaa: 0.0 ± 0.0
Thr
2.248ThrAla: 2.248 ± 0.093
0.372ThrCys: 0.372 ± 0.035
2.47ThrAsp: 2.47 ± 0.088
2.765ThrGlu: 2.765 ± 0.107
3.078ThrPhe: 3.078 ± 0.107
2.984ThrGly: 2.984 ± 0.09
0.674ThrHis: 0.674 ± 0.043
5.429ThrIle: 5.429 ± 0.141
4.948ThrLys: 4.948 ± 0.119
5.276ThrLeu: 5.276 ± 0.14
0.998ThrMet: 0.998 ± 0.061
3.725ThrAsn: 3.725 ± 0.123
1.699ThrPro: 1.699 ± 0.069
1.35ThrGln: 1.35 ± 0.059
1.427ThrArg: 1.427 ± 0.07
3.982ThrSer: 3.982 ± 0.108
3.181ThrThr: 3.181 ± 0.106
2.688ThrVal: 2.688 ± 0.097
0.49ThrTrp: 0.49 ± 0.043
2.068ThrTyr: 2.068 ± 0.078
0.0ThrXaa: 0.0 ± 0.0
Val
3.32ValAla: 3.32 ± 0.121
0.546ValCys: 0.546 ± 0.039
3.498ValAsp: 3.498 ± 0.103
3.935ValGlu: 3.935 ± 0.132
3.046ValPhe: 3.046 ± 0.115
3.45ValGly: 3.45 ± 0.119
0.738ValHis: 0.738 ± 0.052
5.574ValIle: 5.574 ± 0.139
5.169ValLys: 5.169 ± 0.143
5.707ValLeu: 5.707 ± 0.155
1.409ValMet: 1.409 ± 0.067
3.855ValAsn: 3.855 ± 0.129
1.557ValPro: 1.557 ± 0.069
1.182ValGln: 1.182 ± 0.058
1.69ValArg: 1.69 ± 0.076
4.168ValSer: 4.168 ± 0.106
3.028ValThr: 3.028 ± 0.104
3.769ValVal: 3.769 ± 0.124
0.523ValTrp: 0.523 ± 0.038
2.307ValTyr: 2.307 ± 0.092
0.0ValXaa: 0.0 ± 0.0
Trp
0.387TrpAla: 0.387 ± 0.036
0.089TrpCys: 0.089 ± 0.018
0.549TrpAsp: 0.549 ± 0.039
0.597TrpGlu: 0.597 ± 0.046
0.674TrpPhe: 0.674 ± 0.048
0.431TrpGly: 0.431 ± 0.036
0.086TrpHis: 0.086 ± 0.016
0.951TrpIle: 0.951 ± 0.052
1.019TrpLys: 1.019 ± 0.064
1.01TrpLeu: 1.01 ± 0.053
0.298TrpMet: 0.298 ± 0.03
0.753TrpAsn: 0.753 ± 0.061
0.204TrpPro: 0.204 ± 0.022
0.204TrpGln: 0.204 ± 0.022
0.269TrpArg: 0.269 ± 0.028
0.632TrpSer: 0.632 ± 0.046
0.529TrpThr: 0.529 ± 0.043
0.647TrpVal: 0.647 ± 0.046
0.195TrpTrp: 0.195 ± 0.021
0.443TrpTyr: 0.443 ± 0.04
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.79TyrAla: 1.79 ± 0.079
0.378TyrCys: 0.378 ± 0.033
2.508TyrAsp: 2.508 ± 0.086
2.836TyrGlu: 2.836 ± 0.097
2.65TyrPhe: 2.65 ± 0.11
1.973TyrGly: 1.973 ± 0.075
0.535TyrHis: 0.535 ± 0.042
3.716TyrIle: 3.716 ± 0.092
4.431TyrLys: 4.431 ± 0.133
4.384TyrLeu: 4.384 ± 0.117
0.747TyrMet: 0.747 ± 0.049
3.297TyrAsn: 3.297 ± 0.115
0.916TyrPro: 0.916 ± 0.048
1.276TyrGln: 1.276 ± 0.074
1.238TyrArg: 1.238 ± 0.063
2.921TyrSer: 2.921 ± 0.097
1.74TyrThr: 1.74 ± 0.077
2.145TyrVal: 2.145 ± 0.075
0.44TyrTrp: 0.44 ± 0.037
1.725TyrTyr: 1.725 ± 0.091
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 992 proteins (338527 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski