Erysipelatoclostridium sp. An173

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Erysipelotrichia; Erysipelotrichales; Erysipelotrichaceae; Erysipelatoclostridium; unclassified Erysipelatoclostridium

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2792 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y4N1Z1|A0A1Y4N1Z1_9FIRM Glyco_trans_2-like domain-containing protein OS=Erysipelatoclostridium sp. An173 OX=1965571 GN=B5F09_11700 PE=4 SV=1
MM1 pKa = 7.54LKK3 pKa = 10.43KK4 pKa = 10.5LLTIGLSLMMVITLTACGGDD24 pKa = 3.69DD25 pKa = 3.95SNSGSSSSGGSDD37 pKa = 2.95NNTTEE42 pKa = 3.98EE43 pKa = 4.37RR44 pKa = 11.84PFTEE48 pKa = 3.83SKK50 pKa = 11.19GLIKK54 pKa = 10.82YY55 pKa = 7.4FTIEE59 pKa = 4.16GEE61 pKa = 4.38NVCLPEE67 pKa = 4.1TVGEE71 pKa = 4.0YY72 pKa = 10.4VRR74 pKa = 11.84YY75 pKa = 9.8LEE77 pKa = 4.99KK78 pKa = 10.56IGTKK82 pKa = 10.21VEE84 pKa = 4.38LGDD87 pKa = 3.47TGNTVDD93 pKa = 4.79EE94 pKa = 4.92AGEE97 pKa = 3.93LDD99 pKa = 3.45AGGVSSMVAFLKK111 pKa = 10.7VYY113 pKa = 10.33IDD115 pKa = 3.84GASDD119 pKa = 2.78EE120 pKa = 4.42WQWFGIRR127 pKa = 11.84YY128 pKa = 9.67EE129 pKa = 4.05NDD131 pKa = 3.11TDD133 pKa = 3.58EE134 pKa = 4.88SLPIADD140 pKa = 4.74CKK142 pKa = 9.44VTQITLDD149 pKa = 3.36YY150 pKa = 10.14DD151 pKa = 4.25TITEE155 pKa = 4.3EE156 pKa = 4.53TNHH159 pKa = 6.43HH160 pKa = 6.38CLDD163 pKa = 3.52SFVFITQDD171 pKa = 3.4DD172 pKa = 3.93QEE174 pKa = 4.4IAMDD178 pKa = 4.48GDD180 pKa = 4.22TTSSDD185 pKa = 3.37LLDD188 pKa = 4.57YY189 pKa = 11.15IGDD192 pKa = 4.32PYY194 pKa = 11.44QNTDD198 pKa = 3.46GHH200 pKa = 5.17WHH202 pKa = 5.94YY203 pKa = 11.59SDD205 pKa = 3.7DD206 pKa = 3.61QGYY209 pKa = 8.48EE210 pKa = 3.95YY211 pKa = 11.37EE212 pKa = 4.69MVTEE216 pKa = 4.06NKK218 pKa = 10.14KK219 pKa = 10.96GILTRR224 pKa = 11.84VVITYY229 pKa = 9.65PEE231 pKa = 4.35SNN233 pKa = 3.23

Molecular weight: 25.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Y4ND35|A0A1Y4ND35_9FIRM HAD family hydrolase OS=Erysipelatoclostridium sp. An173 OX=1965571 GN=B5F09_00975 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.34QPNKK9 pKa = 8.51RR10 pKa = 11.84KK11 pKa = 9.77RR12 pKa = 11.84SKK14 pKa = 8.62THH16 pKa = 5.5GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVGGRR28 pKa = 11.84KK29 pKa = 9.08VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84KK36 pKa = 9.02RR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.93VLSAA44 pKa = 4.11

Molecular weight: 5.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2792

0

2792

844320

25

4134

302.4

34.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.793 ± 0.046

1.345 ± 0.019

6.241 ± 0.045

6.58 ± 0.048

4.356 ± 0.035

5.908 ± 0.04

1.779 ± 0.022

9.283 ± 0.054

7.965 ± 0.043

9.369 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.671 ± 0.024

6.132 ± 0.046

2.815 ± 0.023

3.422 ± 0.031

3.306 ± 0.037

5.857 ± 0.041

5.088 ± 0.039

6.511 ± 0.039

0.703 ± 0.016

4.878 ± 0.04
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski