Loktanella salsilacus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Loktanella

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3957 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I4HCE3|A0A1I4HCE3_9RHOB Uncharacterized protein OS=Loktanella salsilacus OX=195913 GN=SAMN04488004_11723 PE=4 SV=1
MM1 pKa = 7.45TKK3 pKa = 9.13TVNIFVVAAAAATIAGAAVAQTTTFSNEE31 pKa = 3.53DD32 pKa = 3.17AAADD36 pKa = 3.78QFDD39 pKa = 4.42DD40 pKa = 4.35LQEE43 pKa = 4.3QIADD47 pKa = 3.62DD48 pKa = 4.04AEE50 pKa = 5.1RR51 pKa = 11.84DD52 pKa = 3.4VGRR55 pKa = 11.84FGNEE59 pKa = 3.0GRR61 pKa = 11.84VVGSYY66 pKa = 11.27GSVALRR72 pKa = 11.84ATAYY76 pKa = 11.01NDD78 pKa = 3.82DD79 pKa = 4.01NGSAEE84 pKa = 4.08GTGDD88 pKa = 3.61DD89 pKa = 4.19NANVGLGLRR98 pKa = 11.84YY99 pKa = 8.74GTFDD103 pKa = 3.39GVNGIDD109 pKa = 3.04VTAAYY114 pKa = 10.14NYY116 pKa = 10.72SEE118 pKa = 5.17DD119 pKa = 5.12NGDD122 pKa = 3.48EE123 pKa = 4.27SQNQLLLGADD133 pKa = 3.68YY134 pKa = 11.04RR135 pKa = 11.84RR136 pKa = 11.84DD137 pKa = 3.66FGPKK141 pKa = 8.26YY142 pKa = 10.35FGYY145 pKa = 10.95AKK147 pKa = 10.48LDD149 pKa = 3.61ASQDD153 pKa = 3.35RR154 pKa = 11.84AAEE157 pKa = 4.1NLDD160 pKa = 3.67DD161 pKa = 4.71NYY163 pKa = 10.74IDD165 pKa = 3.78VFVGAGLGYY174 pKa = 10.58RR175 pKa = 11.84ILNDD179 pKa = 3.38ATSQWSIQAGPGYY192 pKa = 10.34RR193 pKa = 11.84YY194 pKa = 8.52TEE196 pKa = 4.3TVAGDD201 pKa = 3.91DD202 pKa = 3.82NSEE205 pKa = 4.0VAASISSNYY214 pKa = 8.71YY215 pKa = 9.21RR216 pKa = 11.84SLSATSYY223 pKa = 8.35VTNDD227 pKa = 2.63TDD229 pKa = 3.85VIYY232 pKa = 10.99SEE234 pKa = 4.46TSTLLTNDD242 pKa = 3.44LALNVAMTNSLSLRR256 pKa = 11.84TSLSTSFDD264 pKa = 3.7DD265 pKa = 5.71AVDD268 pKa = 3.98DD269 pKa = 4.72SFGDD273 pKa = 3.47ASNTLGVAVVYY284 pKa = 10.43NFNN287 pKa = 3.6

Molecular weight: 30.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I4G9A6|A0A1I4G9A6_9RHOB Putative fluoride ion transporter CrcB OS=Loktanella salsilacus OX=195913 GN=crcB PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNRR10 pKa = 11.84VRR12 pKa = 11.84KK13 pKa = 8.99NRR15 pKa = 11.84HH16 pKa = 3.77GFRR19 pKa = 11.84ARR21 pKa = 11.84MATPSGRR28 pKa = 11.84KK29 pKa = 8.93IINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.31GRR39 pKa = 11.84AKK41 pKa = 10.69LSAA44 pKa = 3.92

Molecular weight: 5.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3957

0

3957

1233139

27

4280

311.6

33.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.738 ± 0.065

0.88 ± 0.012

6.563 ± 0.041

4.735 ± 0.043

3.679 ± 0.03

8.57 ± 0.045

1.999 ± 0.024

5.451 ± 0.03

3.027 ± 0.035

9.893 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.903 ± 0.021

2.751 ± 0.026

5.024 ± 0.029

3.498 ± 0.025

6.206 ± 0.037

5.099 ± 0.027

6.083 ± 0.039

7.319 ± 0.033

1.366 ± 0.017

2.216 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski