Candidatus Finniella inopinata
Average proteome isoelectric point is 6.85
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1538 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4Q7DLE7|A0A4Q7DLE7_9PROT Uncharacterized protein OS=Candidatus Finniella inopinata OX=1696036 GN=EQU50_00365 PE=4 SV=1
MM1 pKa = 7.4 GVQTISTAPISCCMGTAPATLTALPISFDD30 pKa = 3.32 LTMVMPAATINDD42 pKa = 4.2 HH43 pKa = 7.31 LPFLNILPFGMCITLGNPLVAAATAAALGILVPLPCLPMTMAPWAPGAPTVQLGGQPALNDD104 pKa = 3.79 SSVLTCMWGAAIAIGSPGQPLTMIPP129 pKa = 3.52
Molecular weight: 12.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.942
IPC2_protein 4.024
IPC_protein 3.656
Toseland 3.503
ProMoST 3.973
Dawson 3.732
Bjellqvist 3.923
Wikipedia 3.834
Rodwell 3.541
Grimsley 3.452
Solomon 3.63
Lehninger 3.592
Nozaki 3.948
DTASelect 4.151
Thurlkill 3.668
EMBOSS 3.821
Sillero 3.821
Patrickios 0.006
IPC_peptide 3.617
IPC2_peptide 3.757
IPC2.peptide.svr19 3.763
Protein with the highest isoelectric point:
>tr|A0A4V2E005|A0A4V2E005_9PROT Uncharacterized protein OS=Candidatus Finniella inopinata OX=1696036 GN=EQU50_01245 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.38 RR3 pKa = 11.84 TYY5 pKa = 10.3 QPSQLVRR12 pKa = 11.84 KK13 pKa = 9.03 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.42 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATVGGRR28 pKa = 11.84 RR29 pKa = 11.84 VIAARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.55 GRR39 pKa = 11.84 TKK41 pKa = 10.86 LSAA44 pKa = 3.61
Molecular weight: 5.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.429
IPC2_protein 11.111
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.13
Rodwell 12.31
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.047
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.084
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1538
0
1538
501607
28
2064
326.1
36.33
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.03 ± 0.068
1.153 ± 0.023
5.211 ± 0.045
5.5 ± 0.068
4.472 ± 0.045
6.743 ± 0.061
2.381 ± 0.028
6.343 ± 0.05
6.055 ± 0.064
10.62 ± 0.079
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.248 ± 0.029
4.116 ± 0.043
4.55 ± 0.043
4.597 ± 0.049
4.659 ± 0.051
6.865 ± 0.065
5.817 ± 0.058
6.311 ± 0.044
1.215 ± 0.023
3.115 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here