Amino acid dipepetide frequency for Nocardioides sp.

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	19.126AlaAla: 19.126 ± 0.178	1.028AlaCys: 1.028 ± 0.028	8.239AlaAsp: 8.239 ± 0.082	8.522AlaGlu: 8.522 ± 0.09	3.52AlaPhe: 3.52 ± 0.059	12.235AlaGly: 12.235 ± 0.097	2.514AlaHis: 2.514 ± 0.042	4.743AlaIle: 4.743 ± 0.069	2.601AlaLys: 2.601 ± 0.051	13.047AlaLeu: 13.047 ± 0.119	2.753AlaMet: 2.753 ± 0.047	2.092AlaAsn: 2.092 ± 0.038	5.977AlaPro: 5.977 ± 0.066	3.454AlaGln: 3.454 ± 0.052	9.473AlaArg: 9.473 ± 0.108	6.444AlaSer: 6.444 ± 0.076	7.405AlaThr: 7.405 ± 0.073	11.714AlaVal: 11.714 ± 0.125	1.935AlaTrp: 1.935 ± 0.039	2.396AlaTyr: 2.396 ± 0.042	0.001AlaXaa: 0.001 ± 0.001
Cys	0.963CysAla: 0.963 ± 0.029	0.096CysCys: 0.096 ± 0.008	0.505CysAsp: 0.505 ± 0.022	0.406CysGlu: 0.406 ± 0.017	0.234CysPhe: 0.234 ± 0.013	0.88CysGly: 0.88 ± 0.026	0.202CysHis: 0.202 ± 0.013	0.219CysIle: 0.219 ± 0.014	0.104CysLys: 0.104 ± 0.009	0.647CysLeu: 0.647 ± 0.022	0.132CysMet: 0.132 ± 0.01	0.153CysAsn: 0.153 ± 0.012	0.499CysPro: 0.499 ± 0.022	0.186CysGln: 0.186 ± 0.012	0.585CysArg: 0.585 ± 0.022	0.446CysSer: 0.446 ± 0.018	0.475CysThr: 0.475 ± 0.022	0.608CysVal: 0.608 ± 0.021	0.13CysTrp: 0.13 ± 0.01	0.163CysTyr: 0.163 ± 0.012	0.0CysXaa: 0.0 ± 0.0
Asp	7.821AspAla: 7.821 ± 0.078	0.428AspCys: 0.428 ± 0.02	4.272AspAsp: 4.272 ± 0.074	4.34AspGlu: 4.34 ± 0.057	1.686AspPhe: 1.686 ± 0.037	6.329AspGly: 6.329 ± 0.069	1.608AspHis: 1.608 ± 0.037	2.095AspIle: 2.095 ± 0.044	1.079AspLys: 1.079 ± 0.032	7.195AspLeu: 7.195 ± 0.068	0.86AspMet: 0.86 ± 0.025	1.063AspAsn: 1.063 ± 0.031	4.659AspPro: 4.659 ± 0.064	1.924AspGln: 1.924 ± 0.04	5.319AspArg: 5.319 ± 0.071	2.473AspSer: 2.473 ± 0.05	2.798AspThr: 2.798 ± 0.045	5.99AspVal: 5.99 ± 0.076	1.0AspTrp: 1.0 ± 0.028	1.199AspTyr: 1.199 ± 0.033	0.0AspXaa: 0.0 ± 0.0
Glu	7.247GluAla: 7.247 ± 0.085	0.38GluCys: 0.38 ± 0.018	3.191GluAsp: 3.191 ± 0.051	3.693GluGlu: 3.693 ± 0.056	1.658GluPhe: 1.658 ± 0.033	4.402GluGly: 4.402 ± 0.054	1.635GluHis: 1.635 ± 0.037	2.712GluIle: 2.712 ± 0.051	1.401GluLys: 1.401 ± 0.038	6.723GluLeu: 6.723 ± 0.075	1.065GluMet: 1.065 ± 0.029	1.038GluAsn: 1.038 ± 0.028	3.333GluPro: 3.333 ± 0.063	2.385GluGln: 2.385 ± 0.045	5.212GluArg: 5.212 ± 0.069	2.836GluSer: 2.836 ± 0.048	2.991GluThr: 2.991 ± 0.054	5.59GluVal: 5.59 ± 0.072	0.864GluTrp: 0.864 ± 0.024	1.154GluTyr: 1.154 ± 0.028	0.0GluXaa: 0.0 ± 0.0
Phe	3.717PheAla: 3.717 ± 0.057	0.286PheCys: 0.286 ± 0.015	2.107PheAsp: 2.107 ± 0.043	1.678PheGlu: 1.678 ± 0.035	0.878PhePhe: 0.878 ± 0.028	3.112PheGly: 3.112 ± 0.05	0.615PheHis: 0.615 ± 0.023	0.821PheIle: 0.821 ± 0.025	0.507PheLys: 0.507 ± 0.016	2.546PheLeu: 2.546 ± 0.047	0.436PheMet: 0.436 ± 0.018	0.614PheAsn: 0.614 ± 0.024	1.324PhePro: 1.324 ± 0.034	0.669PheGln: 0.669 ± 0.021	1.856PheArg: 1.856 ± 0.034	1.488PheSer: 1.488 ± 0.033	1.825PheThr: 1.825 ± 0.037	2.619PheVal: 2.619 ± 0.044	0.444PheTrp: 0.444 ± 0.019	0.643PheTyr: 0.643 ± 0.021	0.0PheXaa: 0.0 ± 0.0
Gly	10.331GlyAla: 10.331 ± 0.09	0.833GlyCys: 0.833 ± 0.029	5.478GlyAsp: 5.478 ± 0.072	5.277GlyGlu: 5.277 ± 0.069	2.963GlyPhe: 2.963 ± 0.051	8.107GlyGly: 8.107 ± 0.096	2.09GlyHis: 2.09 ± 0.039	3.867GlyIle: 3.867 ± 0.061	2.088GlyLys: 2.088 ± 0.046	9.442GlyLeu: 9.442 ± 0.087	2.037GlyMet: 2.037 ± 0.038	1.754GlyAsn: 1.754 ± 0.046	4.54GlyPro: 4.54 ± 0.065	2.572GlyGln: 2.572 ± 0.053	7.405GlyArg: 7.405 ± 0.077	5.368GlySer: 5.368 ± 0.068	5.52GlyThr: 5.52 ± 0.075	8.036GlyVal: 8.036 ± 0.084	1.708GlyTrp: 1.708 ± 0.039	2.249GlyTyr: 2.249 ± 0.044	0.0GlyXaa: 0.0 ± 0.0
His	2.544HisAla: 2.544 ± 0.048	0.191HisCys: 0.191 ± 0.013	1.497HisAsp: 1.497 ± 0.036	1.223HisGlu: 1.223 ± 0.034	0.644HisPhe: 0.644 ± 0.021	2.285HisGly: 2.285 ± 0.042	0.729HisHis: 0.729 ± 0.026	0.58HisIle: 0.58 ± 0.023	0.339HisLys: 0.339 ± 0.015	2.388HisLeu: 2.388 ± 0.042	0.336HisMet: 0.336 ± 0.016	0.384HisAsn: 0.384 ± 0.017	1.677HisPro: 1.677 ± 0.041	0.676HisGln: 0.676 ± 0.022	1.943HisArg: 1.943 ± 0.039	0.922HisSer: 0.922 ± 0.031	1.144HisThr: 1.144 ± 0.029	1.942HisVal: 1.942 ± 0.041	0.313HisTrp: 0.313 ± 0.017	0.465HisTyr: 0.465 ± 0.019	0.0HisXaa: 0.0 ± 0.0
Ile	5.396IleAla: 5.396 ± 0.069	0.324IleCys: 0.324 ± 0.016	2.863IleAsp: 2.863 ± 0.047	2.556IleGlu: 2.556 ± 0.042	0.896IlePhe: 0.896 ± 0.027	3.926IleGly: 3.926 ± 0.056	0.68IleHis: 0.68 ± 0.021	1.11IleIle: 1.11 ± 0.035	0.794IleLys: 0.794 ± 0.026	2.861IleLeu: 2.861 ± 0.05	0.468IleMet: 0.468 ± 0.017	0.865IleAsn: 0.865 ± 0.027	1.953IlePro: 1.953 ± 0.04	0.847IleGln: 0.847 ± 0.025	2.551IleArg: 2.551 ± 0.037	1.958IleSer: 1.958 ± 0.034	2.342IleThr: 2.342 ± 0.046	3.386IleVal: 3.386 ± 0.053	0.432IleTrp: 0.432 ± 0.017	0.666IleTyr: 0.666 ± 0.023	0.0IleXaa: 0.0 ± 0.0
Lys	2.61LysAla: 2.61 ± 0.05	0.09LysCys: 0.09 ± 0.008	1.169LysAsp: 1.169 ± 0.033	1.108LysGlu: 1.108 ± 0.031	0.456LysPhe: 0.456 ± 0.019	1.574LysGly: 1.574 ± 0.036	0.442LysHis: 0.442 ± 0.02	0.812LysIle: 0.812 ± 0.027	0.646LysLys: 0.646 ± 0.031	1.805LysLeu: 1.805 ± 0.039	0.368LysMet: 0.368 ± 0.021	0.442LysAsn: 0.442 ± 0.018	1.243LysPro: 1.243 ± 0.033	0.688LysGln: 0.688 ± 0.026	1.492LysArg: 1.492 ± 0.037	1.073LysSer: 1.073 ± 0.028	1.1LysThr: 1.1 ± 0.03	1.989LysVal: 1.989 ± 0.046	0.227LysTrp: 0.227 ± 0.012	0.424LysTyr: 0.424 ± 0.016	0.0LysXaa: 0.0 ± 0.0
Leu	14.686LeuAla: 14.686 ± 0.127	0.764LeuCys: 0.764 ± 0.024	6.808LeuAsp: 6.808 ± 0.074	5.661LeuGlu: 5.661 ± 0.069	2.495LeuPhe: 2.495 ± 0.052	9.523LeuGly: 9.523 ± 0.085	2.071LeuHis: 2.071 ± 0.038	3.248LeuIle: 3.248 ± 0.041	1.777LeuLys: 1.777 ± 0.039	10.516LeuLeu: 10.516 ± 0.13	1.713LeuMet: 1.713 ± 0.038	1.665LeuAsn: 1.665 ± 0.035	5.649LeuPro: 5.649 ± 0.066	2.46LeuGln: 2.46 ± 0.052	7.936LeuArg: 7.936 ± 0.094	5.255LeuSer: 5.255 ± 0.067	6.387LeuThr: 6.387 ± 0.072	9.988LeuVal: 9.988 ± 0.095	1.22LeuTrp: 1.22 ± 0.032	1.573LeuTyr: 1.573 ± 0.04	0.0LeuXaa: 0.0 ± 0.0
Met	2.328MetAla: 2.328 ± 0.043	0.153MetCys: 0.153 ± 0.01	0.879MetAsp: 0.879 ± 0.023	0.822MetGlu: 0.822 ± 0.025	0.485MetPhe: 0.485 ± 0.02	1.413MetGly: 1.413 ± 0.033	0.377MetHis: 0.377 ± 0.016	0.73MetIle: 0.73 ± 0.025	0.462MetLys: 0.462 ± 0.016	1.876MetLeu: 1.876 ± 0.04	0.333MetMet: 0.333 ± 0.017	0.397MetAsn: 0.397 ± 0.015	1.115MetPro: 1.115 ± 0.03	0.47MetGln: 0.47 ± 0.022	1.558MetArg: 1.558 ± 0.034	1.426MetSer: 1.426 ± 0.025	1.694MetThr: 1.694 ± 0.034	1.609MetVal: 1.609 ± 0.035	0.216MetTrp: 0.216 ± 0.014	0.258MetTyr: 0.258 ± 0.014	0.0MetXaa: 0.0 ± 0.0
Asn	2.23AsnAla: 2.23 ± 0.043	0.168AsnCys: 0.168 ± 0.01	1.141AsnAsp: 1.141 ± 0.032	0.932AsnGlu: 0.932 ± 0.025	0.496AsnPhe: 0.496 ± 0.022	1.831AsnGly: 1.831 ± 0.043	0.407AsnHis: 0.407 ± 0.019	0.686AsnIle: 0.686 ± 0.026	0.384AsnLys: 0.384 ± 0.018	1.845AsnLeu: 1.845 ± 0.036	0.287AsnMet: 0.287 ± 0.016	0.46AsnAsn: 0.46 ± 0.021	1.469AsnPro: 1.469 ± 0.033	0.578AsnGln: 0.578 ± 0.023	1.382AsnArg: 1.382 ± 0.032	0.833AsnSer: 0.833 ± 0.027	1.031AsnThr: 1.031 ± 0.033	1.594AsnVal: 1.594 ± 0.041	0.284AsnTrp: 0.284 ± 0.014	0.426AsnTyr: 0.426 ± 0.018	0.0AsnXaa: 0.0 ± 0.0
Pro	7.11ProAla: 7.11 ± 0.072	0.305ProCys: 0.305 ± 0.016	4.408ProAsp: 4.408 ± 0.062	4.11ProGlu: 4.11 ± 0.06	1.551ProPhe: 1.551 ± 0.029	5.758ProGly: 5.758 ± 0.073	1.243ProHis: 1.243 ± 0.032	1.825ProIle: 1.825 ± 0.034	1.07ProLys: 1.07 ± 0.03	4.794ProLeu: 4.794 ± 0.059	1.059ProMet: 1.059 ± 0.027	0.908ProAsn: 0.908 ± 0.026	3.054ProPro: 3.054 ± 0.065	1.477ProGln: 1.477 ± 0.037	3.601ProArg: 3.601 ± 0.048	3.078ProSer: 3.078 ± 0.042	3.508ProThr: 3.508 ± 0.054	5.224ProVal: 5.224 ± 0.072	0.883ProTrp: 0.883 ± 0.021	1.151ProTyr: 1.151 ± 0.03	0.0ProXaa: 0.0 ± 0.0
Gln	3.52GlnAla: 3.52 ± 0.05	0.15GlnCys: 0.15 ± 0.011	1.324GlnAsp: 1.324 ± 0.035	1.417GlnGlu: 1.417 ± 0.034	0.755GlnPhe: 0.755 ± 0.026	2.053GlnGly: 2.053 ± 0.035	0.723GlnHis: 0.723 ± 0.024	1.218GlnIle: 1.218 ± 0.032	0.562GlnLys: 0.562 ± 0.02	3.172GlnLeu: 3.172 ± 0.054	0.557GlnMet: 0.557 ± 0.018	0.486GlnAsn: 0.486 ± 0.019	1.673GlnPro: 1.673 ± 0.035	1.194GlnGln: 1.194 ± 0.028	2.569GlnArg: 2.569 ± 0.051	1.219GlnSer: 1.219 ± 0.028	1.408GlnThr: 1.408 ± 0.033	2.833GlnVal: 2.833 ± 0.039	0.45GlnTrp: 0.45 ± 0.017	0.569GlnTyr: 0.569 ± 0.022	0.0GlnXaa: 0.0 ± 0.0
Arg	9.156ArgAla: 9.156 ± 0.112	0.554ArgCys: 0.554 ± 0.023	4.561ArgAsp: 4.561 ± 0.057	4.502ArgGlu: 4.502 ± 0.057	2.372ArgPhe: 2.372 ± 0.045	5.792ArgGly: 5.792 ± 0.072	1.938ArgHis: 1.938 ± 0.039	3.431ArgIle: 3.431 ± 0.053	1.54ArgLys: 1.54 ± 0.035	8.304ArgLeu: 8.304 ± 0.086	1.834ArgMet: 1.834 ± 0.034	1.479ArgAsn: 1.479 ± 0.033	4.32ArgPro: 4.32 ± 0.063	2.277ArgGln: 2.277 ± 0.041	7.494ArgArg: 7.494 ± 0.103	4.408ArgSer: 4.408 ± 0.06	4.586ArgThr: 4.586 ± 0.065	6.359ArgVal: 6.359 ± 0.075	1.401ArgTrp: 1.401 ± 0.034	1.684ArgTyr: 1.684 ± 0.035	0.001ArgXaa: 0.001 ± 0.001
Ser	6.432SerAla: 6.432 ± 0.071	0.384SerCys: 0.384 ± 0.019	3.046SerAsp: 3.046 ± 0.05	2.671SerGlu: 2.671 ± 0.052	1.747SerPhe: 1.747 ± 0.033	5.501SerGly: 5.501 ± 0.073	1.099SerHis: 1.099 ± 0.028	1.905SerIle: 1.905 ± 0.04	0.994SerLys: 0.994 ± 0.028	4.904SerLeu: 4.904 ± 0.06	1.189SerMet: 1.189 ± 0.028	1.001SerAsn: 1.001 ± 0.027	3.025SerPro: 3.025 ± 0.053	1.313SerGln: 1.313 ± 0.031	3.836SerArg: 3.836 ± 0.049	3.161SerSer: 3.161 ± 0.061	3.306SerThr: 3.306 ± 0.059	4.448SerVal: 4.448 ± 0.061	0.942SerTrp: 0.942 ± 0.025	1.468SerTyr: 1.468 ± 0.036	0.0SerXaa: 0.0 ± 0.0
Thr	7.634ThrAla: 7.634 ± 0.088	0.446ThrCys: 0.446 ± 0.022	3.659ThrAsp: 3.659 ± 0.048	3.106ThrGlu: 3.106 ± 0.049	1.825ThrPhe: 1.825 ± 0.04	6.094ThrGly: 6.094 ± 0.089	1.192ThrHis: 1.192 ± 0.024	2.31ThrIle: 2.31 ± 0.038	1.114ThrLys: 1.114 ± 0.029	5.507ThrLeu: 5.507 ± 0.073	1.048ThrMet: 1.048 ± 0.026	1.139ThrAsn: 1.139 ± 0.036	3.786ThrPro: 3.786 ± 0.057	1.373ThrGln: 1.373 ± 0.033	3.905ThrArg: 3.905 ± 0.052	3.387ThrSer: 3.387 ± 0.052	4.003ThrThr: 4.003 ± 0.064	5.726ThrVal: 5.726 ± 0.08	0.997ThrTrp: 0.997 ± 0.023	1.382ThrTyr: 1.382 ± 0.046	0.0ThrXaa: 0.0 ± 0.0
Val	12.339ValAla: 12.339 ± 0.103	0.713ValCys: 0.713 ± 0.026	6.319ValAsp: 6.319 ± 0.066	5.679ValGlu: 5.679 ± 0.067	2.329ValPhe: 2.329 ± 0.049	7.718ValGly: 7.718 ± 0.069	1.965ValHis: 1.965 ± 0.043	3.445ValIle: 3.445 ± 0.055	1.642ValLys: 1.642 ± 0.042	9.854ValLeu: 9.854 ± 0.106	1.621ValMet: 1.621 ± 0.034	1.792ValAsn: 1.792 ± 0.039	5.064ValPro: 5.064 ± 0.071	2.195ValGln: 2.195 ± 0.036	6.914ValArg: 6.914 ± 0.08	4.563ValSer: 4.563 ± 0.059	6.048ValThr: 6.048 ± 0.078	10.103ValVal: 10.103 ± 0.113	1.131ValTrp: 1.131 ± 0.032	1.537ValTyr: 1.537 ± 0.031	0.0ValXaa: 0.0 ± 0.0
Trp	1.627TrpAla: 1.627 ± 0.038	0.152TrpCys: 0.152 ± 0.01	0.87TrpAsp: 0.87 ± 0.026	0.725TrpGlu: 0.725 ± 0.022	0.532TrpPhe: 0.532 ± 0.021	1.087TrpGly: 1.087 ± 0.028	0.349TrpHis: 0.349 ± 0.015	0.601TrpIle: 0.601 ± 0.021	0.312TrpLys: 0.312 ± 0.017	1.82TrpLeu: 1.82 ± 0.038	0.308TrpMet: 0.308 ± 0.016	0.354TrpAsn: 0.354 ± 0.016	0.737TrpPro: 0.737 ± 0.022	0.523TrpGln: 0.523 ± 0.021	1.297TrpArg: 1.297 ± 0.033	1.024TrpSer: 1.024 ± 0.027	1.0TrpThr: 1.0 ± 0.028	1.285TrpVal: 1.285 ± 0.036	0.346TrpTrp: 0.346 ± 0.018	0.29TrpTyr: 0.29 ± 0.015	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.399TyrAla: 2.399 ± 0.046	0.188TyrCys: 0.188 ± 0.012	1.802TyrAsp: 1.802 ± 0.034	1.083TyrGlu: 1.083 ± 0.029	0.663TyrPhe: 0.663 ± 0.024	2.043TyrGly: 2.043 ± 0.042	0.344TyrHis: 0.344 ± 0.016	0.518TyrIle: 0.518 ± 0.02	0.341TyrLys: 0.341 ± 0.015	2.149TyrLeu: 2.149 ± 0.041	0.198TyrMet: 0.198 ± 0.012	0.377TyrAsn: 0.377 ± 0.02	1.021TyrPro: 1.021 ± 0.026	0.591TyrGln: 0.591 ± 0.023	1.664TyrArg: 1.664 ± 0.033	1.011TyrSer: 1.011 ± 0.03	1.06TyrThr: 1.06 ± 0.039	1.928TyrVal: 1.928 ± 0.032	0.317TyrTrp: 0.317 ± 0.016	0.468TyrTyr: 0.468 ± 0.02	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.001XaaPro: 0.001 ± 0.001	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4389 proteins (1381642 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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