Sulfitobacter sabulilitoris
Average proteome isoelectric point is 6.22
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3675 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5S3Q331|A0A5S3Q331_9RHOB DUF2842 domain-containing protein OS=Sulfitobacter sabulilitoris OX=2562655 GN=FDT80_16365 PE=4 SV=1
MM1 pKa = 7.12 QLADD5 pKa = 3.54 YY6 pKa = 9.91 FYY8 pKa = 10.95 GYY10 pKa = 10.64 GFAGDD15 pKa = 4.39 YY16 pKa = 7.58 FTGAAIDD23 pKa = 3.9 LSRR26 pKa = 11.84 EE27 pKa = 3.99 GGGTQFLVGGSASQQSNPGNEE48 pKa = 4.06 FHH50 pKa = 6.64 FTPVAGGLAYY60 pKa = 10.38 FQGNGFLNPSTGTVNSVTVDD80 pKa = 3.47 PLGGNTARR88 pKa = 11.84 FGIQGFSQNLATMQTATWAFYY109 pKa = 7.64 EE110 pKa = 4.32 TNIFNGNDD118 pKa = 3.34 TMTGSYY124 pKa = 10.7 QNDD127 pKa = 3.57 RR128 pKa = 11.84 LVGYY132 pKa = 10.51 AGDD135 pKa = 4.63 DD136 pKa = 3.44 VLFDD140 pKa = 4.08 GKK142 pKa = 11.05 GDD144 pKa = 3.44 KK145 pKa = 10.48 KK146 pKa = 10.82 NYY148 pKa = 8.51 NVSVTPYY155 pKa = 9.85 PDD157 pKa = 3.64 PTPSTLRR164 pKa = 11.84 NPGEE168 pKa = 4.44 TVSNTSFFVDD178 pKa = 4.82 DD179 pKa = 4.95 GDD181 pKa = 4.72 DD182 pKa = 3.78 VLDD185 pKa = 4.47 GEE187 pKa = 4.95 AGNDD191 pKa = 3.46 RR192 pKa = 11.84 LDD194 pKa = 3.78 GGTGNDD200 pKa = 3.98 LLIGGSGNDD209 pKa = 3.75 EE210 pKa = 3.96 LWGGGTGNDD219 pKa = 3.83 TLNGGTGNDD228 pKa = 3.79 TLHH231 pKa = 6.68 GGSGIDD237 pKa = 2.98 TAVFNVASTDD247 pKa = 3.14 ITGG250 pKa = 3.21
Molecular weight: 25.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.689
IPC2_protein 3.643
IPC_protein 3.668
Toseland 3.427
ProMoST 3.859
Dawson 3.681
Bjellqvist 3.834
Wikipedia 3.668
Rodwell 3.49
Grimsley 3.338
Solomon 3.668
Lehninger 3.63
Nozaki 3.795
DTASelect 4.101
Thurlkill 3.503
EMBOSS 3.668
Sillero 3.795
Patrickios 1.036
IPC_peptide 3.656
IPC2_peptide 3.757
IPC2.peptide.svr19 3.715
Protein with the highest isoelectric point:
>tr|A0A5S3P7Z1|A0A5S3P7Z1_9RHOB PAS domain S-box protein OS=Sulfitobacter sabulilitoris OX=2562655 GN=FDT80_17640 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 KK13 pKa = 9.18 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.42 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.37 AGRR28 pKa = 11.84 KK29 pKa = 8.81 IINARR34 pKa = 11.84 RR35 pKa = 11.84 AQGRR39 pKa = 11.84 KK40 pKa = 9.34 SLSAA44 pKa = 3.83
Molecular weight: 5.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.199
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.647
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.954
DTASelect 12.954
Thurlkill 12.954
EMBOSS 13.466
Sillero 12.954
Patrickios 12.369
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.154
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3675
0
3675
1186263
33
3284
322.8
34.93
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.751 ± 0.049
0.886 ± 0.012
6.517 ± 0.039
5.152 ± 0.037
3.6 ± 0.022
8.817 ± 0.046
2.115 ± 0.02
5.072 ± 0.028
2.852 ± 0.028
9.903 ± 0.047
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.842 ± 0.024
2.438 ± 0.024
5.179 ± 0.035
3.276 ± 0.021
7.02 ± 0.042
4.977 ± 0.028
5.668 ± 0.029
7.378 ± 0.032
1.368 ± 0.017
2.188 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here