Chitinimonas arctica
Average proteome isoelectric point is 6.68
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4560 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A516SIH2|A0A516SIH2_9BURK Uncharacterized protein OS=Chitinimonas arctica OX=2594795 GN=FNU76_17260 PE=4 SV=1
MM1 pKa = 6.92 NAAVEE6 pKa = 4.35 VEE8 pKa = 4.51 TPFVFTDD15 pKa = 3.19 NAAAKK20 pKa = 10.02 VADD23 pKa = 5.43 LIAEE27 pKa = 4.31 EE28 pKa = 4.82 GNPDD32 pKa = 2.99 LKK34 pKa = 11.14 LRR36 pKa = 11.84 VFVTGGGCSGFQYY49 pKa = 10.94 GFTFDD54 pKa = 4.81 EE55 pKa = 4.64 ITNEE59 pKa = 4.11 DD60 pKa = 3.73 DD61 pKa = 4.42 TPFTKK66 pKa = 10.68 NGVTLLVDD74 pKa = 3.69 PMSYY78 pKa = 10.34 QYY80 pKa = 11.57 LVGAEE85 pKa = 3.7 IDD87 pKa = 3.79 YY88 pKa = 11.15 VEE90 pKa = 5.03 SIEE93 pKa = 4.92 GSQFTIKK100 pKa = 10.61 NPNASSTCGCGSSFSVV116 pKa = 3.54
Molecular weight: 12.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.751
IPC2_protein 3.846
IPC_protein 3.757
Toseland 3.567
ProMoST 3.923
Dawson 3.745
Bjellqvist 3.897
Wikipedia 3.668
Rodwell 3.592
Grimsley 3.49
Solomon 3.719
Lehninger 3.668
Nozaki 3.872
DTASelect 4.037
Thurlkill 3.63
EMBOSS 3.681
Sillero 3.872
Patrickios 0.769
IPC_peptide 3.719
IPC2_peptide 3.859
IPC2.peptide.svr19 3.794
Protein with the highest isoelectric point:
>tr|A0A516SFF6|A0A516SFF6_9BURK tRNA-dihydrouridine synthase B OS=Chitinimonas arctica OX=2594795 GN=dusB PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.36 RR3 pKa = 11.84 TYY5 pKa = 9.6 QPSVIRR11 pKa = 11.84 RR12 pKa = 11.84 KK13 pKa = 8.64 RR14 pKa = 11.84 THH16 pKa = 5.95 GFLVRR21 pKa = 11.84 SRR23 pKa = 11.84 TRR25 pKa = 11.84 GGRR28 pKa = 11.84 AVLAARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.11 GRR39 pKa = 11.84 HH40 pKa = 4.94 SLSVV44 pKa = 3.04
Molecular weight: 5.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.428
IPC2_protein 11.111
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.13
Rodwell 12.281
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.018
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.094
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4560
0
4560
1522789
34
7695
333.9
36.44
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.01 ± 0.053
0.947 ± 0.014
5.614 ± 0.048
5.487 ± 0.038
3.474 ± 0.022
8.196 ± 0.064
2.134 ± 0.02
4.616 ± 0.028
3.695 ± 0.034
11.188 ± 0.059
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.285 ± 0.02
3.109 ± 0.038
4.863 ± 0.036
4.243 ± 0.027
6.66 ± 0.038
5.72 ± 0.034
4.964 ± 0.043
6.812 ± 0.032
1.411 ± 0.016
2.57 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here