Salmonella phage P46FS4
Average proteome isoelectric point is 6.14
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 212 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6G6XUX0|A0A6G6XUX0_9CAUD SegD-like protein OS=Salmonella phage P46FS4 OX=2712940 GN=P46FS4_190 PE=4 SV=1
MM1 pKa = 7.61 FIEE4 pKa = 3.95 QLVYY8 pKa = 10.61 LGAPEE13 pKa = 4.23 AVFEE17 pKa = 4.26 FRR19 pKa = 11.84 DD20 pKa = 4.52 PITPAVDD27 pKa = 3.5 RR28 pKa = 11.84 SLLAVQQCLVFPEE41 pKa = 4.9 GVEE44 pKa = 4.11 PPTSVILNNLPQSVQSGTQLPTFTFACIQAYY75 pKa = 10.18 LFII78 pKa = 6.39
Molecular weight: 8.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.905
IPC2_protein 4.151
IPC_protein 3.859
Toseland 3.732
ProMoST 4.024
Dawson 3.834
Bjellqvist 3.999
Wikipedia 3.745
Rodwell 3.719
Grimsley 3.656
Solomon 3.783
Lehninger 3.745
Nozaki 3.986
DTASelect 4.037
Thurlkill 3.795
EMBOSS 3.757
Sillero 3.986
Patrickios 0.477
IPC_peptide 3.795
IPC2_peptide 3.961
IPC2.peptide.svr19 3.885
Protein with the highest isoelectric point:
>tr|A0A6G6XTE6|A0A6G6XTE6_9CAUD Putative DNA repair/recombination protein OS=Salmonella phage P46FS4 OX=2712940 GN=P46FS4_36 PE=4 SV=1
MM1 pKa = 7.6 EE2 pKa = 5.61 IEE4 pKa = 4.19 EE5 pKa = 4.46 VATFADD11 pKa = 5.33 FLASRR16 pKa = 11.84 MDD18 pKa = 3.51 EE19 pKa = 3.81 QKK21 pKa = 11.29 VIDD24 pKa = 4.09 KK25 pKa = 10.98 VNAKK29 pKa = 9.55 GKK31 pKa = 9.56 RR32 pKa = 11.84 RR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 LQCAPGFKK43 pKa = 10.4 LSADD47 pKa = 3.72 GSRR50 pKa = 11.84 CEE52 pKa = 3.98 VMVASEE58 pKa = 3.79 RR59 pKa = 11.84 RR60 pKa = 11.84 TRR62 pKa = 11.84 KK63 pKa = 9.58 LGNRR67 pKa = 11.84 KK68 pKa = 9.64 AIRR71 pKa = 11.84 AKK73 pKa = 10.19 KK74 pKa = 9.33 RR75 pKa = 11.84 MGMGYY80 pKa = 8.62 SRR82 pKa = 11.84 KK83 pKa = 9.18 IEE85 pKa = 4.0 RR86 pKa = 11.84 RR87 pKa = 11.84 KK88 pKa = 9.83 KK89 pKa = 9.75 KK90 pKa = 10.38 AMKK93 pKa = 8.73 FRR95 pKa = 11.84 KK96 pKa = 9.24 MMGLSS101 pKa = 3.27
Molecular weight: 11.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.258
IPC2_protein 9.867
IPC_protein 10.906
Toseland 11.33
ProMoST 11.462
Dawson 11.345
Bjellqvist 11.169
Wikipedia 11.667
Rodwell 11.535
Grimsley 11.374
Solomon 11.652
Lehninger 11.594
Nozaki 11.301
DTASelect 11.169
Thurlkill 11.301
EMBOSS 11.769
Sillero 11.316
Patrickios 11.257
IPC_peptide 11.667
IPC2_peptide 10.438
IPC2.peptide.svr19 9.009
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
212
0
212
48782
32
1612
230.1
25.86
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.206 ± 0.191
1.15 ± 0.072
6.547 ± 0.114
6.48 ± 0.162
4.303 ± 0.121
6.931 ± 0.228
1.845 ± 0.103
6.078 ± 0.105
6.261 ± 0.173
8.103 ± 0.157
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.884 ± 0.104
4.879 ± 0.146
3.958 ± 0.114
3.782 ± 0.113
4.949 ± 0.137
5.943 ± 0.151
6.119 ± 0.2
7.458 ± 0.163
1.351 ± 0.072
3.774 ± 0.122
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here