Amino acid dipepetide frequency for Salmonella phage P46FS4

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.191AlaAla: 6.191 ± 0.426
0.676AlaCys: 0.676 ± 0.103
4.325AlaAsp: 4.325 ± 0.32
4.756AlaGlu: 4.756 ± 0.416
2.521AlaPhe: 2.521 ± 0.236
5.391AlaGly: 5.391 ± 0.428
1.414AlaHis: 1.414 ± 0.205
4.715AlaIle: 4.715 ± 0.289
4.366AlaLys: 4.366 ± 0.304
6.047AlaLeu: 6.047 ± 0.38
1.906AlaMet: 1.906 ± 0.211
3.239AlaAsn: 3.239 ± 0.254
2.972AlaPro: 2.972 ± 0.314
2.849AlaGln: 2.849 ± 0.288
3.362AlaArg: 3.362 ± 0.257
3.895AlaSer: 3.895 ± 0.333
4.715AlaThr: 4.715 ± 0.398
5.289AlaVal: 5.289 ± 0.406
0.881AlaTrp: 0.881 ± 0.128
2.542AlaTyr: 2.542 ± 0.244
0.0AlaXaa: 0.0 ± 0.0
Cys
0.717CysAla: 0.717 ± 0.103
0.164CysCys: 0.164 ± 0.057
0.881CysAsp: 0.881 ± 0.134
0.799CysGlu: 0.799 ± 0.114
0.348CysPhe: 0.348 ± 0.077
0.717CysGly: 0.717 ± 0.14
0.266CysHis: 0.266 ± 0.08
0.84CysIle: 0.84 ± 0.133
0.738CysLys: 0.738 ± 0.109
0.861CysLeu: 0.861 ± 0.152
0.287CysMet: 0.287 ± 0.074
0.574CysAsn: 0.574 ± 0.099
0.574CysPro: 0.574 ± 0.117
0.348CysGln: 0.348 ± 0.086
0.553CysArg: 0.553 ± 0.123
0.799CysSer: 0.799 ± 0.136
0.574CysThr: 0.574 ± 0.12
0.943CysVal: 0.943 ± 0.132
0.143CysTrp: 0.143 ± 0.056
0.369CysTyr: 0.369 ± 0.1
0.0CysXaa: 0.0 ± 0.0
Asp
4.94AspAla: 4.94 ± 0.391
0.635AspCys: 0.635 ± 0.118
3.997AspAsp: 3.997 ± 0.362
4.202AspGlu: 4.202 ± 0.299
3.403AspPhe: 3.403 ± 0.269
5.289AspGly: 5.289 ± 0.312
0.963AspHis: 0.963 ± 0.122
4.407AspIle: 4.407 ± 0.293
3.567AspLys: 3.567 ± 0.256
5.391AspLeu: 5.391 ± 0.375
2.009AspMet: 2.009 ± 0.194
2.562AspAsn: 2.562 ± 0.248
3.054AspPro: 3.054 ± 0.255
2.173AspGln: 2.173 ± 0.222
2.808AspArg: 2.808 ± 0.259
3.444AspSer: 3.444 ± 0.265
3.649AspThr: 3.649 ± 0.211
4.571AspVal: 4.571 ± 0.315
0.984AspTrp: 0.984 ± 0.13
3.423AspTyr: 3.423 ± 0.295
0.0AspXaa: 0.0 ± 0.0
Glu
4.612GluAla: 4.612 ± 0.386
0.676GluCys: 0.676 ± 0.128
4.141GluAsp: 4.141 ± 0.369
4.264GluGlu: 4.264 ± 0.358
2.911GluPhe: 2.911 ± 0.228
4.243GluGly: 4.243 ± 0.286
1.353GluHis: 1.353 ± 0.154
3.936GluIle: 3.936 ± 0.338
3.505GluLys: 3.505 ± 0.341
6.088GluLeu: 6.088 ± 0.375
2.193GluMet: 2.193 ± 0.192
2.747GluAsn: 2.747 ± 0.257
2.05GluPro: 2.05 ± 0.196
2.685GluGln: 2.685 ± 0.22
3.464GluArg: 3.464 ± 0.297
3.3GluSer: 3.3 ± 0.289
3.874GluThr: 3.874 ± 0.307
4.92GluVal: 4.92 ± 0.369
1.168GluTrp: 1.168 ± 0.152
2.665GluTyr: 2.665 ± 0.226
0.0GluXaa: 0.0 ± 0.0
Phe
2.48PheAla: 2.48 ± 0.216
0.389PheCys: 0.389 ± 0.094
2.685PheAsp: 2.685 ± 0.25
3.28PheGlu: 3.28 ± 0.284
1.742PhePhe: 1.742 ± 0.21
3.054PheGly: 3.054 ± 0.24
0.963PheHis: 0.963 ± 0.146
2.706PheIle: 2.706 ± 0.209
2.624PheLys: 2.624 ± 0.275
2.931PheLeu: 2.931 ± 0.218
1.414PheMet: 1.414 ± 0.171
2.48PheAsn: 2.48 ± 0.256
1.619PhePro: 1.619 ± 0.223
1.558PheGln: 1.558 ± 0.175
2.214PheArg: 2.214 ± 0.231
2.89PheSer: 2.89 ± 0.245
2.931PheThr: 2.931 ± 0.251
2.808PheVal: 2.808 ± 0.236
0.615PheTrp: 0.615 ± 0.111
1.64PheTyr: 1.64 ± 0.18
0.0PheXaa: 0.0 ± 0.0
Gly
4.182GlyAla: 4.182 ± 0.284
1.045GlyCys: 1.045 ± 0.149
3.854GlyAsp: 3.854 ± 0.288
4.51GlyGlu: 4.51 ± 0.275
3.034GlyPhe: 3.034 ± 0.264
5.227GlyGly: 5.227 ± 0.461
1.517GlyHis: 1.517 ± 0.191
4.858GlyIle: 4.858 ± 0.33
4.797GlyLys: 4.797 ± 0.31
5.268GlyLeu: 5.268 ± 0.35
1.927GlyMet: 1.927 ± 0.168
3.628GlyAsn: 3.628 ± 0.415
1.23GlyPro: 1.23 ± 0.139
2.747GlyGln: 2.747 ± 0.215
3.177GlyArg: 3.177 ± 0.232
4.612GlySer: 4.612 ± 0.368
4.202GlyThr: 4.202 ± 0.365
5.801GlyVal: 5.801 ± 0.422
1.271GlyTrp: 1.271 ± 0.182
2.419GlyTyr: 2.419 ± 0.235
0.0GlyXaa: 0.0 ± 0.0
His
1.209HisAla: 1.209 ± 0.142
0.266HisCys: 0.266 ± 0.072
1.271HisAsp: 1.271 ± 0.182
0.779HisGlu: 0.779 ± 0.113
0.902HisPhe: 0.902 ± 0.127
1.107HisGly: 1.107 ± 0.163
0.512HisHis: 0.512 ± 0.109
1.353HisIle: 1.353 ± 0.19
1.353HisLys: 1.353 ± 0.162
1.64HisLeu: 1.64 ± 0.162
0.574HisMet: 0.574 ± 0.099
0.779HisAsn: 0.779 ± 0.123
0.902HisPro: 0.902 ± 0.139
0.594HisGln: 0.594 ± 0.112
1.025HisArg: 1.025 ± 0.137
0.963HisSer: 0.963 ± 0.162
1.148HisThr: 1.148 ± 0.187
1.066HisVal: 1.066 ± 0.171
0.246HisTrp: 0.246 ± 0.082
0.758HisTyr: 0.758 ± 0.127
0.0HisXaa: 0.0 ± 0.0
Ile
4.182IleAla: 4.182 ± 0.299
0.615IleCys: 0.615 ± 0.125
5.104IleAsp: 5.104 ± 0.314
4.858IleGlu: 4.858 ± 0.334
1.968IlePhe: 1.968 ± 0.21
3.915IleGly: 3.915 ± 0.281
1.189IleHis: 1.189 ± 0.151
3.3IleIle: 3.3 ± 0.321
3.731IleLys: 3.731 ± 0.234
4.1IleLeu: 4.1 ± 0.277
1.517IleMet: 1.517 ± 0.19
3.362IleAsn: 3.362 ± 0.277
2.931IlePro: 2.931 ± 0.249
2.706IleGln: 2.706 ± 0.237
3.28IleArg: 3.28 ± 0.295
3.116IleSer: 3.116 ± 0.264
4.264IleThr: 4.264 ± 0.272
4.059IleVal: 4.059 ± 0.242
0.656IleTrp: 0.656 ± 0.119
1.927IleTyr: 1.927 ± 0.216
0.0IleXaa: 0.0 ± 0.0
Lys
4.305LysAla: 4.305 ± 0.354
0.717LysCys: 0.717 ± 0.142
4.284LysAsp: 4.284 ± 0.281
4.243LysGlu: 4.243 ± 0.355
2.931LysPhe: 2.931 ± 0.251
4.387LysGly: 4.387 ± 0.339
1.107LysHis: 1.107 ± 0.176
3.649LysIle: 3.649 ± 0.244
4.038LysLys: 4.038 ± 0.413
5.022LysLeu: 5.022 ± 0.296
2.583LysMet: 2.583 ± 0.284
2.48LysAsn: 2.48 ± 0.231
2.501LysPro: 2.501 ± 0.253
2.439LysGln: 2.439 ± 0.215
3.341LysArg: 3.341 ± 0.302
3.895LysSer: 3.895 ± 0.245
3.341LysThr: 3.341 ± 0.287
4.448LysVal: 4.448 ± 0.284
0.984LysTrp: 0.984 ± 0.13
1.906LysTyr: 1.906 ± 0.187
0.0LysXaa: 0.0 ± 0.0
Leu
6.355LeuAla: 6.355 ± 0.357
0.82LeuCys: 0.82 ± 0.117
5.473LeuAsp: 5.473 ± 0.356
4.981LeuGlu: 4.981 ± 0.353
3.485LeuPhe: 3.485 ± 0.255
5.166LeuGly: 5.166 ± 0.326
1.373LeuHis: 1.373 ± 0.166
3.936LeuIle: 3.936 ± 0.306
6.375LeuLys: 6.375 ± 0.422
6.539LeuLeu: 6.539 ± 0.48
2.132LeuMet: 2.132 ± 0.192
4.284LeuAsn: 4.284 ± 0.333
3.608LeuPro: 3.608 ± 0.29
2.911LeuGln: 2.911 ± 0.258
3.69LeuArg: 3.69 ± 0.266
5.227LeuSer: 5.227 ± 0.273
4.899LeuThr: 4.899 ± 0.373
6.047LeuVal: 6.047 ± 0.382
0.676LeuTrp: 0.676 ± 0.142
3.054LeuTyr: 3.054 ± 0.298
0.0LeuXaa: 0.0 ± 0.0
Met
2.48MetAla: 2.48 ± 0.25
0.307MetCys: 0.307 ± 0.086
1.599MetAsp: 1.599 ± 0.173
1.373MetGlu: 1.373 ± 0.167
1.599MetPhe: 1.599 ± 0.204
1.742MetGly: 1.742 ± 0.18
0.41MetHis: 0.41 ± 0.109
1.558MetIle: 1.558 ± 0.137
2.173MetLys: 2.173 ± 0.233
2.562MetLeu: 2.562 ± 0.241
0.861MetMet: 0.861 ± 0.137
1.558MetAsn: 1.558 ± 0.187
0.984MetPro: 0.984 ± 0.152
1.107MetGln: 1.107 ± 0.155
1.64MetArg: 1.64 ± 0.168
2.07MetSer: 2.07 ± 0.2
1.763MetThr: 1.763 ± 0.166
1.947MetVal: 1.947 ± 0.194
0.246MetTrp: 0.246 ± 0.062
0.861MetTyr: 0.861 ± 0.122
0.0MetXaa: 0.0 ± 0.0
Asn
3.792AsnAla: 3.792 ± 0.28
0.615AsnCys: 0.615 ± 0.129
2.624AsnAsp: 2.624 ± 0.23
2.337AsnGlu: 2.337 ± 0.251
2.152AsnPhe: 2.152 ± 0.2
4.059AsnGly: 4.059 ± 0.276
0.963AsnHis: 0.963 ± 0.164
3.054AsnIle: 3.054 ± 0.247
2.665AsnLys: 2.665 ± 0.179
3.772AsnLeu: 3.772 ± 0.296
1.394AsnMet: 1.394 ± 0.186
2.87AsnAsn: 2.87 ± 0.331
2.296AsnPro: 2.296 ± 0.214
1.681AsnGln: 1.681 ± 0.179
2.337AsnArg: 2.337 ± 0.219
2.583AsnSer: 2.583 ± 0.217
3.116AsnThr: 3.116 ± 0.279
3.731AsnVal: 3.731 ± 0.29
0.635AsnTrp: 0.635 ± 0.133
2.111AsnTyr: 2.111 ± 0.21
0.0AsnXaa: 0.0 ± 0.0
Pro
2.849ProAla: 2.849 ± 0.235
0.471ProCys: 0.471 ± 0.108
2.993ProAsp: 2.993 ± 0.245
3.382ProGlu: 3.382 ± 0.278
1.886ProPhe: 1.886 ± 0.203
2.398ProGly: 2.398 ± 0.212
0.635ProHis: 0.635 ± 0.101
1.763ProIle: 1.763 ± 0.161
2.357ProLys: 2.357 ± 0.228
2.952ProLeu: 2.952 ± 0.243
0.984ProMet: 0.984 ± 0.119
1.742ProAsn: 1.742 ± 0.193
1.312ProPro: 1.312 ± 0.204
1.537ProGln: 1.537 ± 0.185
1.701ProArg: 1.701 ± 0.193
2.849ProSer: 2.849 ± 0.271
2.665ProThr: 2.665 ± 0.276
3.239ProVal: 3.239 ± 0.31
0.553ProTrp: 0.553 ± 0.102
1.312ProTyr: 1.312 ± 0.177
0.0ProXaa: 0.0 ± 0.0
Gln
2.89GlnAla: 2.89 ± 0.321
0.389GlnCys: 0.389 ± 0.095
2.521GlnAsp: 2.521 ± 0.231
2.296GlnGlu: 2.296 ± 0.301
1.824GlnPhe: 1.824 ± 0.189
2.337GlnGly: 2.337 ± 0.207
0.615GlnHis: 0.615 ± 0.119
2.337GlnIle: 2.337 ± 0.201
2.255GlnLys: 2.255 ± 0.18
2.993GlnLeu: 2.993 ± 0.266
1.168GlnMet: 1.168 ± 0.14
1.578GlnAsn: 1.578 ± 0.18
1.291GlnPro: 1.291 ± 0.143
1.66GlnGln: 1.66 ± 0.24
1.968GlnArg: 1.968 ± 0.215
2.214GlnSer: 2.214 ± 0.219
2.398GlnThr: 2.398 ± 0.199
2.726GlnVal: 2.726 ± 0.232
0.553GlnTrp: 0.553 ± 0.113
1.804GlnTyr: 1.804 ± 0.169
0.0GlnXaa: 0.0 ± 0.0
Arg
3.013ArgAla: 3.013 ± 0.21
0.717ArgCys: 0.717 ± 0.136
3.013ArgAsp: 3.013 ± 0.238
3.095ArgGlu: 3.095 ± 0.276
2.193ArgPhe: 2.193 ± 0.244
3.198ArgGly: 3.198 ± 0.223
1.025ArgHis: 1.025 ± 0.146
3.28ArgIle: 3.28 ± 0.235
3.136ArgLys: 3.136 ± 0.282
4.612ArgLeu: 4.612 ± 0.334
1.824ArgMet: 1.824 ± 0.207
2.378ArgAsn: 2.378 ± 0.236
1.845ArgPro: 1.845 ± 0.222
1.865ArgGln: 1.865 ± 0.191
3.3ArgArg: 3.3 ± 0.305
2.562ArgSer: 2.562 ± 0.217
2.665ArgThr: 2.665 ± 0.214
3.075ArgVal: 3.075 ± 0.24
0.717ArgTrp: 0.717 ± 0.114
1.947ArgTyr: 1.947 ± 0.194
0.0ArgXaa: 0.0 ± 0.0
Ser
4.12SerAla: 4.12 ± 0.3
0.512SerCys: 0.512 ± 0.146
3.915SerAsp: 3.915 ± 0.255
3.382SerGlu: 3.382 ± 0.241
2.439SerPhe: 2.439 ± 0.222
4.633SerGly: 4.633 ± 0.396
0.84SerHis: 0.84 ± 0.146
4.018SerIle: 4.018 ± 0.263
3.69SerLys: 3.69 ± 0.263
5.166SerLeu: 5.166 ± 0.358
1.414SerMet: 1.414 ± 0.144
3.259SerAsn: 3.259 ± 0.328
2.255SerPro: 2.255 ± 0.219
2.05SerGln: 2.05 ± 0.199
2.788SerArg: 2.788 ± 0.231
3.936SerSer: 3.936 ± 0.373
3.485SerThr: 3.485 ± 0.323
4.489SerVal: 4.489 ± 0.308
0.553SerTrp: 0.553 ± 0.114
2.48SerTyr: 2.48 ± 0.238
0.0SerXaa: 0.0 ± 0.0
Thr
4.551ThrAla: 4.551 ± 0.378
0.758ThrCys: 0.758 ± 0.127
3.526ThrAsp: 3.526 ± 0.245
4.038ThrGlu: 4.038 ± 0.251
2.685ThrPhe: 2.685 ± 0.253
4.653ThrGly: 4.653 ± 0.419
1.045ThrHis: 1.045 ± 0.144
3.874ThrIle: 3.874 ± 0.284
3.526ThrLys: 3.526 ± 0.233
5.104ThrLeu: 5.104 ± 0.399
1.127ThrMet: 1.127 ± 0.154
2.87ThrAsn: 2.87 ± 0.253
3.587ThrPro: 3.587 ± 0.291
1.988ThrGln: 1.988 ± 0.202
3.013ThrArg: 3.013 ± 0.242
3.28ThrSer: 3.28 ± 0.26
3.854ThrThr: 3.854 ± 0.366
4.981ThrVal: 4.981 ± 0.313
0.861ThrTrp: 0.861 ± 0.134
1.865ThrTyr: 1.865 ± 0.203
0.0ThrXaa: 0.0 ± 0.0
Val
4.961ValAla: 4.961 ± 0.321
0.922ValCys: 0.922 ± 0.158
5.555ValAsp: 5.555 ± 0.334
5.043ValGlu: 5.043 ± 0.351
2.89ValPhe: 2.89 ± 0.288
4.407ValGly: 4.407 ± 0.271
1.066ValHis: 1.066 ± 0.135
4.346ValIle: 4.346 ± 0.321
5.002ValLys: 5.002 ± 0.331
5.76ValLeu: 5.76 ± 0.334
2.009ValMet: 2.009 ± 0.188
3.731ValAsn: 3.731 ± 0.302
2.665ValPro: 2.665 ± 0.208
2.808ValGln: 2.808 ± 0.284
3.177ValArg: 3.177 ± 0.286
4.879ValSer: 4.879 ± 0.364
4.92ValThr: 4.92 ± 0.4
6.375ValVal: 6.375 ± 0.462
1.127ValTrp: 1.127 ± 0.166
2.931ValTyr: 2.931 ± 0.245
0.0ValXaa: 0.0 ± 0.0
Trp
1.148TrpAla: 1.148 ± 0.155
0.287TrpCys: 0.287 ± 0.085
0.881TrpAsp: 0.881 ± 0.152
1.148TrpGlu: 1.148 ± 0.133
0.615TrpPhe: 0.615 ± 0.107
0.861TrpGly: 0.861 ± 0.132
0.246TrpHis: 0.246 ± 0.064
0.738TrpIle: 0.738 ± 0.13
0.758TrpLys: 0.758 ± 0.129
1.291TrpLeu: 1.291 ± 0.144
0.389TrpMet: 0.389 ± 0.098
0.676TrpAsn: 0.676 ± 0.122
0.41TrpPro: 0.41 ± 0.096
0.41TrpGln: 0.41 ± 0.081
0.902TrpArg: 0.902 ± 0.14
0.676TrpSer: 0.676 ± 0.112
0.533TrpThr: 0.533 ± 0.098
0.902TrpVal: 0.902 ± 0.134
0.184TrpTrp: 0.184 ± 0.055
0.451TrpTyr: 0.451 ± 0.102
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.075TyrAla: 3.075 ± 0.231
0.512TyrCys: 0.512 ± 0.121
2.829TyrAsp: 2.829 ± 0.252
2.029TyrGlu: 2.029 ± 0.22
1.496TyrPhe: 1.496 ± 0.154
2.521TyrGly: 2.521 ± 0.238
0.943TyrHis: 0.943 ± 0.13
2.111TyrIle: 2.111 ± 0.219
1.947TyrLys: 1.947 ± 0.181
2.931TyrLeu: 2.931 ± 0.237
1.107TyrMet: 1.107 ± 0.126
1.988TyrAsn: 1.988 ± 0.204
1.496TyrPro: 1.496 ± 0.17
1.701TyrGln: 1.701 ± 0.186
1.742TyrArg: 1.742 ± 0.204
2.234TyrSer: 2.234 ± 0.189
2.193TyrThr: 2.193 ± 0.26
3.157TyrVal: 3.157 ± 0.242
0.451TyrTrp: 0.451 ± 0.113
1.271TyrTyr: 1.271 ± 0.161
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 212 proteins (48782 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski