Ethanoligenens harbinense (strain DSM 18485 / JCM 12961 / CGMCC 1.5033 / YUAN-3)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; Ethanoligenens; Ethanoligenens harbinense

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2663 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E6U6M0|E6U6M0_ETHHY Nucleoid-associated protein Ethha_0166 OS=Ethanoligenens harbinense (strain DSM 18485 / JCM 12961 / CGMCC 1.5033 / YUAN-3) OX=663278 GN=Ethha_0166 PE=3 SV=1
MM1 pKa = 7.69ASLNTGLNTALSGLSASQTALGVTAHH27 pKa = 6.72NISNAATDD35 pKa = 4.71GYY37 pKa = 9.79SRR39 pKa = 11.84QMVDD43 pKa = 3.37LQAVNVNTGAYY54 pKa = 6.73QWKK57 pKa = 8.92PVVPFVGNGVNSNQITQARR76 pKa = 11.84DD77 pKa = 2.77GFLDD81 pKa = 3.29VRR83 pKa = 11.84YY84 pKa = 8.16RR85 pKa = 11.84TANAQYY91 pKa = 10.66SDD93 pKa = 3.74YY94 pKa = 10.83EE95 pKa = 3.99QRR97 pKa = 11.84QNDD100 pKa = 3.77LGQVEE105 pKa = 5.61DD106 pKa = 4.04IFNEE110 pKa = 4.13VSTSGNDD117 pKa = 3.47TLQGLSGQLNTLINDD132 pKa = 3.35IGSYY136 pKa = 9.63QSSPASSSLPVTIKK150 pKa = 9.93TDD152 pKa = 3.39AEE154 pKa = 4.11NLVTKK159 pKa = 10.47IRR161 pKa = 11.84TAYY164 pKa = 10.53SSLTTFEE171 pKa = 4.33SQEE174 pKa = 3.8QSALNVVVSGDD185 pKa = 3.78TQSGGINGILQNISTLNTQIVSMEE209 pKa = 4.25VGGQPANDD217 pKa = 4.01LRR219 pKa = 11.84DD220 pKa = 3.49QRR222 pKa = 11.84NQLLDD227 pKa = 3.61KK228 pKa = 11.09LSGYY232 pKa = 10.68LDD234 pKa = 3.54ISATEE239 pKa = 3.87QSDD242 pKa = 3.89GSVTVQMEE250 pKa = 3.91NDD252 pKa = 3.61LASGSGAMLVDD263 pKa = 4.19GNNIVHH269 pKa = 5.9TLKK272 pKa = 10.85VVTDD276 pKa = 4.69GPTDD280 pKa = 3.53ASGLSTTAVTWDD292 pKa = 3.71ASDD295 pKa = 4.49SNNQSTTYY303 pKa = 9.25ATNTGTGWSGNTLSVGGGSVNGYY326 pKa = 9.38LAILNGDD333 pKa = 3.55GSGTGSYY340 pKa = 11.07GDD342 pKa = 3.68VGVHH346 pKa = 5.48YY347 pKa = 10.53LKK349 pKa = 10.89EE350 pKa = 4.07HH351 pKa = 6.63LNDD354 pKa = 4.04FAQSFADD361 pKa = 3.43IMNSTSTSSGGGAQMLTYY379 pKa = 10.79GSGTPPTPTYY389 pKa = 10.43PDD391 pKa = 3.3TTGWSDD397 pKa = 3.39PTASAAYY404 pKa = 9.65VATDD408 pKa = 3.71AAATISLSDD417 pKa = 3.6AWNKK421 pKa = 10.99DD422 pKa = 3.04DD423 pKa = 5.54TLFGDD428 pKa = 4.17NYY430 pKa = 9.8TGTSVGTYY438 pKa = 9.96AQAFLNALGSAQVSSYY454 pKa = 9.6DD455 pKa = 3.56TSAHH459 pKa = 5.37KK460 pKa = 9.05TLYY463 pKa = 10.1TDD465 pKa = 3.25GTMASYY471 pKa = 10.87AATFSNKK478 pKa = 8.6IANVINDD485 pKa = 4.15DD486 pKa = 3.21TTMAGNYY493 pKa = 9.09QIQKK497 pKa = 10.31NDD499 pKa = 4.02LDD501 pKa = 3.95TQRR504 pKa = 11.84QSVSSVSIDD513 pKa = 3.49EE514 pKa = 4.26EE515 pKa = 4.8TVNLMKK521 pKa = 10.38FQQMYY526 pKa = 8.93GASARR531 pKa = 11.84VITTINDD538 pKa = 3.25MMGTLLSMAQQ548 pKa = 3.16

Molecular weight: 57.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E6U542|E6U542_ETHHY DUF2344 domain-containing protein OS=Ethanoligenens harbinense (strain DSM 18485 / JCM 12961 / CGMCC 1.5033 / YUAN-3) OX=663278 GN=Ethha_1197 PE=4 SV=1
MM1 pKa = 7.62IKK3 pKa = 9.2TPRR6 pKa = 11.84NQPPRR11 pKa = 11.84IKK13 pKa = 10.39EE14 pKa = 3.84RR15 pKa = 11.84LHH17 pKa = 6.89AFLLTSGASFLLLLLLFSPQAGLDD41 pKa = 3.64GARR44 pKa = 11.84TGLLLCAEE52 pKa = 4.49VVIPTLFPFLVLSSFVIHH70 pKa = 6.87LGLAEE75 pKa = 3.94RR76 pKa = 11.84FGRR79 pKa = 11.84LFTRR83 pKa = 11.84LTAALFKK90 pKa = 11.05LPGASAPVLVLGILGGYY107 pKa = 8.5PVGARR112 pKa = 11.84ACAEE116 pKa = 4.03LVDD119 pKa = 4.16TGALTRR125 pKa = 11.84AEE127 pKa = 4.36GNRR130 pKa = 11.84LLTFCINSSPAYY142 pKa = 9.73IIGAAGAGLLHH153 pKa = 6.73SAQAGALLYY162 pKa = 10.12AAHH165 pKa = 7.11ILSSLLVGTLLGMHH179 pKa = 6.26SPKK182 pKa = 10.29PSAGALARR190 pKa = 11.84RR191 pKa = 11.84PVTRR195 pKa = 11.84MPASGALVTSVTGAATSMLGISGFVVFFSSLSSLLFSTGLVGWMARR241 pKa = 11.84LAAFLPGLHH250 pKa = 6.28WFDD253 pKa = 4.59AGTAQTLLKK262 pKa = 10.81GFLEE266 pKa = 4.45VSGGCDD272 pKa = 2.69AAVHH276 pKa = 6.1NGQFVLEE283 pKa = 4.24ALSAFLSWSGLSVVFQVLFAVRR305 pKa = 11.84GTGLSARR312 pKa = 11.84GYY314 pKa = 8.05VASRR318 pKa = 11.84PLHH321 pKa = 5.31MLFSILFTVILFSLFPVAIPTLAATSRR348 pKa = 11.84VALSMHH354 pKa = 6.55NAPASAALLFACALLLLARR373 pKa = 11.84ATVV376 pKa = 3.18

Molecular weight: 39.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2663

0

2663

838389

31

3906

314.8

34.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.012 ± 0.064

1.397 ± 0.021

5.457 ± 0.039

5.829 ± 0.062

4.095 ± 0.032

7.801 ± 0.044

2.01 ± 0.021

5.972 ± 0.049

4.944 ± 0.042

9.705 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.532 ± 0.022

3.674 ± 0.04

4.119 ± 0.028

3.567 ± 0.033

5.566 ± 0.062

5.97 ± 0.069

5.773 ± 0.062

7.315 ± 0.042

0.913 ± 0.014

3.349 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski