Amino acid dipepetide frequency for Ethanoligenens harbinense (strain DSM 18485 / JCM 12961 / CGMCC 1.5033 / YUAN-3)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.636AlaAla: 12.636 ± 0.19
1.435AlaCys: 1.435 ± 0.046
5.887AlaAsp: 5.887 ± 0.094
5.687AlaGlu: 5.687 ± 0.1
4.247AlaPhe: 4.247 ± 0.088
8.528AlaGly: 8.528 ± 0.118
1.904AlaHis: 1.904 ± 0.055
4.969AlaIle: 4.969 ± 0.09
4.339AlaLys: 4.339 ± 0.092
10.457AlaLeu: 10.457 ± 0.137
2.353AlaMet: 2.353 ± 0.059
2.951AlaAsn: 2.951 ± 0.067
3.403AlaPro: 3.403 ± 0.079
3.879AlaGln: 3.879 ± 0.096
5.458AlaArg: 5.458 ± 0.105
5.173AlaSer: 5.173 ± 0.11
4.195AlaThr: 4.195 ± 0.086
8.77AlaVal: 8.77 ± 0.124
0.725AlaTrp: 0.725 ± 0.032
3.119AlaTyr: 3.119 ± 0.066
0.0AlaXaa: 0.0 ± 0.0
Cys
1.543CysAla: 1.543 ± 0.055
0.24CysCys: 0.24 ± 0.017
0.776CysAsp: 0.776 ± 0.033
0.78CysGlu: 0.78 ± 0.034
0.493CysPhe: 0.493 ± 0.025
1.506CysGly: 1.506 ± 0.052
0.284CysHis: 0.284 ± 0.017
0.841CysIle: 0.841 ± 0.033
0.599CysLys: 0.599 ± 0.029
1.073CysLeu: 1.073 ± 0.039
0.371CysMet: 0.371 ± 0.023
0.457CysAsn: 0.457 ± 0.022
0.714CysPro: 0.714 ± 0.032
0.283CysGln: 0.283 ± 0.023
0.89CysArg: 0.89 ± 0.034
0.778CysSer: 0.778 ± 0.032
0.821CysThr: 0.821 ± 0.036
0.982CysVal: 0.982 ± 0.036
0.104CysTrp: 0.104 ± 0.011
0.435CysTyr: 0.435 ± 0.021
0.0CysXaa: 0.0 ± 0.0
Asp
5.787AspAla: 5.787 ± 0.087
0.81AspCys: 0.81 ± 0.033
2.802AspAsp: 2.802 ± 0.074
3.5AspGlu: 3.5 ± 0.07
2.455AspPhe: 2.455 ± 0.054
4.975AspGly: 4.975 ± 0.091
0.891AspHis: 0.891 ± 0.033
3.845AspIle: 3.845 ± 0.076
2.483AspLys: 2.483 ± 0.062
4.609AspLeu: 4.609 ± 0.063
1.499AspMet: 1.499 ± 0.04
1.739AspAsn: 1.739 ± 0.041
2.228AspPro: 2.228 ± 0.058
1.256AspGln: 1.256 ± 0.044
2.82AspArg: 2.82 ± 0.071
2.678AspSer: 2.678 ± 0.063
3.391AspThr: 3.391 ± 0.062
4.146AspVal: 4.146 ± 0.078
0.593AspTrp: 0.593 ± 0.028
2.062AspTyr: 2.062 ± 0.054
0.0AspXaa: 0.0 ± 0.0
Glu
5.192GluAla: 5.192 ± 0.084
0.497GluCys: 0.497 ± 0.028
2.946GluAsp: 2.946 ± 0.065
4.141GluGlu: 4.141 ± 0.092
1.633GluPhe: 1.633 ± 0.049
3.538GluGly: 3.538 ± 0.066
1.288GluHis: 1.288 ± 0.041
3.931GluIle: 3.931 ± 0.088
4.369GluLys: 4.369 ± 0.091
5.507GluLeu: 5.507 ± 0.096
1.656GluMet: 1.656 ± 0.049
2.971GluAsn: 2.971 ± 0.059
2.017GluPro: 2.017 ± 0.053
3.014GluGln: 3.014 ± 0.078
3.658GluArg: 3.658 ± 0.087
2.902GluSer: 2.902 ± 0.06
3.576GluThr: 3.576 ± 0.068
3.279GluVal: 3.279 ± 0.065
0.5GluTrp: 0.5 ± 0.025
1.672GluTyr: 1.672 ± 0.048
0.0GluXaa: 0.0 ± 0.0
Phe
3.986PheAla: 3.986 ± 0.081
0.736PheCys: 0.736 ± 0.029
2.362PheAsp: 2.362 ± 0.053
2.27PheGlu: 2.27 ± 0.065
1.888PhePhe: 1.888 ± 0.059
3.349PheGly: 3.349 ± 0.068
0.94PheHis: 0.94 ± 0.04
2.321PheIle: 2.321 ± 0.058
1.571PheLys: 1.571 ± 0.05
4.191PheLeu: 4.191 ± 0.097
0.852PheMet: 0.852 ± 0.032
1.403PheAsn: 1.403 ± 0.046
1.52PhePro: 1.52 ± 0.044
1.2PheGln: 1.2 ± 0.033
1.914PheArg: 1.914 ± 0.052
3.167PheSer: 3.167 ± 0.074
2.482PheThr: 2.482 ± 0.074
2.852PheVal: 2.852 ± 0.069
0.42PheTrp: 0.42 ± 0.022
1.528PheTyr: 1.528 ± 0.046
0.0PheXaa: 0.0 ± 0.0
Gly
7.003GlyAla: 7.003 ± 0.102
1.277GlyCys: 1.277 ± 0.04
3.799GlyAsp: 3.799 ± 0.07
4.21GlyGlu: 4.21 ± 0.08
3.254GlyPhe: 3.254 ± 0.067
6.471GlyGly: 6.471 ± 0.137
1.527GlyHis: 1.527 ± 0.046
5.299GlyIle: 5.299 ± 0.085
4.501GlyLys: 4.501 ± 0.071
6.972GlyLeu: 6.972 ± 0.088
2.457GlyMet: 2.457 ± 0.053
2.897GlyAsn: 2.897 ± 0.073
1.919GlyPro: 1.919 ± 0.05
2.659GlyGln: 2.659 ± 0.049
4.329GlyArg: 4.329 ± 0.081
4.654GlySer: 4.654 ± 0.112
4.929GlyThr: 4.929 ± 0.108
6.052GlyVal: 6.052 ± 0.089
0.84GlyTrp: 0.84 ± 0.034
2.958GlyTyr: 2.958 ± 0.059
0.0GlyXaa: 0.0 ± 0.0
His
2.006HisAla: 2.006 ± 0.059
0.295HisCys: 0.295 ± 0.02
1.041HisAsp: 1.041 ± 0.037
0.997HisGlu: 0.997 ± 0.04
0.97HisPhe: 0.97 ± 0.033
1.619HisGly: 1.619 ± 0.053
0.469HisHis: 0.469 ± 0.026
1.388HisIle: 1.388 ± 0.043
0.787HisLys: 0.787 ± 0.03
1.868HisLeu: 1.868 ± 0.049
0.542HisMet: 0.542 ± 0.026
0.672HisAsn: 0.672 ± 0.027
1.15HisPro: 1.15 ± 0.036
0.558HisGln: 0.558 ± 0.027
1.059HisArg: 1.059 ± 0.036
1.029HisSer: 1.029 ± 0.033
1.288HisThr: 1.288 ± 0.043
1.447HisVal: 1.447 ± 0.039
0.203HisTrp: 0.203 ± 0.017
0.71HisTyr: 0.71 ± 0.026
0.0HisXaa: 0.0 ± 0.0
Ile
5.839IleAla: 5.839 ± 0.093
0.92IleCys: 0.92 ± 0.032
3.464IleAsp: 3.464 ± 0.068
3.281IleGlu: 3.281 ± 0.071
2.273IlePhe: 2.273 ± 0.064
4.637IleGly: 4.637 ± 0.085
1.243IleHis: 1.243 ± 0.041
3.433IleIle: 3.433 ± 0.076
2.587IleLys: 2.587 ± 0.061
5.977IleLeu: 5.977 ± 0.092
1.19IleMet: 1.19 ± 0.039
2.154IleAsn: 2.154 ± 0.064
2.81IlePro: 2.81 ± 0.062
1.953IleGln: 1.953 ± 0.052
3.744IleArg: 3.744 ± 0.078
3.98IleSer: 3.98 ± 0.077
3.612IleThr: 3.612 ± 0.077
4.267IleVal: 4.267 ± 0.08
0.539IleTrp: 0.539 ± 0.028
1.818IleTyr: 1.818 ± 0.05
0.0IleXaa: 0.0 ± 0.0
Lys
4.585LysAla: 4.585 ± 0.082
0.488LysCys: 0.488 ± 0.025
2.699LysAsp: 2.699 ± 0.067
3.336LysGlu: 3.336 ± 0.067
1.257LysPhe: 1.257 ± 0.04
3.248LysGly: 3.248 ± 0.059
0.904LysHis: 0.904 ± 0.034
3.096LysIle: 3.096 ± 0.067
3.407LysLys: 3.407 ± 0.078
4.407LysLeu: 4.407 ± 0.072
1.379LysMet: 1.379 ± 0.042
2.322LysAsn: 2.322 ± 0.057
2.054LysPro: 2.054 ± 0.053
2.128LysGln: 2.128 ± 0.06
2.817LysArg: 2.817 ± 0.063
2.87LysSer: 2.87 ± 0.061
3.428LysThr: 3.428 ± 0.067
2.877LysVal: 2.877 ± 0.063
0.475LysTrp: 0.475 ± 0.026
1.662LysTyr: 1.662 ± 0.045
0.0LysXaa: 0.0 ± 0.0
Leu
9.542LeuAla: 9.542 ± 0.128
1.645LeuCys: 1.645 ± 0.055
5.252LeuAsp: 5.252 ± 0.08
5.527LeuGlu: 5.527 ± 0.1
4.553LeuPhe: 4.553 ± 0.099
6.561LeuGly: 6.561 ± 0.096
2.221LeuHis: 2.221 ± 0.053
5.121LeuIle: 5.121 ± 0.089
4.522LeuLys: 4.522 ± 0.075
10.313LeuLeu: 10.313 ± 0.178
2.157LeuMet: 2.157 ± 0.052
3.342LeuAsn: 3.342 ± 0.059
4.9LeuPro: 4.9 ± 0.091
3.249LeuGln: 3.249 ± 0.072
6.051LeuArg: 6.051 ± 0.106
6.855LeuSer: 6.855 ± 0.097
5.383LeuThr: 5.383 ± 0.096
5.96LeuVal: 5.96 ± 0.101
0.841LeuTrp: 0.841 ± 0.034
3.061LeuTyr: 3.061 ± 0.059
0.0LeuXaa: 0.0 ± 0.0
Met
2.505MetAla: 2.505 ± 0.049
0.243MetCys: 0.243 ± 0.018
1.539MetAsp: 1.539 ± 0.046
1.735MetGlu: 1.735 ± 0.049
0.771MetPhe: 0.771 ± 0.035
1.812MetGly: 1.812 ± 0.054
0.509MetHis: 0.509 ± 0.024
1.295MetIle: 1.295 ± 0.046
1.515MetLys: 1.515 ± 0.04
2.58MetLeu: 2.58 ± 0.059
0.627MetMet: 0.627 ± 0.03
1.001MetAsn: 1.001 ± 0.03
1.235MetPro: 1.235 ± 0.038
1.187MetGln: 1.187 ± 0.04
1.546MetArg: 1.546 ± 0.051
1.433MetSer: 1.433 ± 0.04
1.401MetThr: 1.401 ± 0.043
1.676MetVal: 1.676 ± 0.046
0.172MetTrp: 0.172 ± 0.016
0.537MetTyr: 0.537 ± 0.028
0.0MetXaa: 0.0 ± 0.0
Asn
3.601AsnAla: 3.601 ± 0.07
0.487AsnCys: 0.487 ± 0.023
1.912AsnAsp: 1.912 ± 0.049
1.842AsnGlu: 1.842 ± 0.046
1.215AsnPhe: 1.215 ± 0.035
3.454AsnGly: 3.454 ± 0.094
0.741AsnHis: 0.741 ± 0.035
2.564AsnIle: 2.564 ± 0.059
1.486AsnLys: 1.486 ± 0.043
3.379AsnLeu: 3.379 ± 0.068
0.942AsnMet: 0.942 ± 0.033
1.379AsnAsn: 1.379 ± 0.051
2.01AsnPro: 2.01 ± 0.051
1.134AsnGln: 1.134 ± 0.04
2.062AsnArg: 2.062 ± 0.051
2.005AsnSer: 2.005 ± 0.059
2.264AsnThr: 2.264 ± 0.063
2.586AsnVal: 2.586 ± 0.058
0.409AsnTrp: 0.409 ± 0.026
1.269AsnTyr: 1.269 ± 0.047
0.0AsnXaa: 0.0 ± 0.0
Pro
4.207ProAla: 4.207 ± 0.098
0.522ProCys: 0.522 ± 0.027
2.993ProAsp: 2.993 ± 0.065
3.05ProGlu: 3.05 ± 0.073
1.904ProPhe: 1.904 ± 0.052
3.143ProGly: 3.143 ± 0.063
0.868ProHis: 0.868 ± 0.028
1.956ProIle: 1.956 ± 0.044
1.749ProLys: 1.749 ± 0.049
3.613ProLeu: 3.613 ± 0.065
0.912ProMet: 0.912 ± 0.035
1.404ProAsn: 1.404 ± 0.047
1.573ProPro: 1.573 ± 0.058
1.542ProGln: 1.542 ± 0.039
1.703ProArg: 1.703 ± 0.046
2.345ProSer: 2.345 ± 0.057
1.955ProThr: 1.955 ± 0.06
3.876ProVal: 3.876 ± 0.081
0.372ProTrp: 0.372 ± 0.019
1.5ProTyr: 1.5 ± 0.05
0.0ProXaa: 0.0 ± 0.0
Gln
3.91GlnAla: 3.91 ± 0.091
0.34GlnCys: 0.34 ± 0.019
1.651GlnAsp: 1.651 ± 0.043
1.86GlnGlu: 1.86 ± 0.046
1.291GlnPhe: 1.291 ± 0.042
2.155GlnGly: 2.155 ± 0.06
0.601GlnHis: 0.601 ± 0.028
2.376GlnIle: 2.376 ± 0.054
2.458GlnLys: 2.458 ± 0.059
2.939GlnLeu: 2.939 ± 0.074
1.102GlnMet: 1.102 ± 0.037
1.855GlnAsn: 1.855 ± 0.052
1.304GlnPro: 1.304 ± 0.04
1.448GlnGln: 1.448 ± 0.055
1.634GlnArg: 1.634 ± 0.048
2.265GlnSer: 2.265 ± 0.069
2.485GlnThr: 2.485 ± 0.059
2.351GlnVal: 2.351 ± 0.053
0.349GlnTrp: 0.349 ± 0.019
1.299GlnTyr: 1.299 ± 0.047
0.0GlnXaa: 0.0 ± 0.0
Arg
5.242ArgAla: 5.242 ± 0.108
0.742ArgCys: 0.742 ± 0.031
2.71ArgAsp: 2.71 ± 0.06
3.799ArgGlu: 3.799 ± 0.085
2.529ArgPhe: 2.529 ± 0.06
3.39ArgGly: 3.39 ± 0.075
1.189ArgHis: 1.189 ± 0.042
3.516ArgIle: 3.516 ± 0.077
3.001ArgLys: 3.001 ± 0.069
5.855ArgLeu: 5.855 ± 0.097
1.724ArgMet: 1.724 ± 0.048
1.799ArgAsn: 1.799 ± 0.054
2.221ArgPro: 2.221 ± 0.057
2.307ArgGln: 2.307 ± 0.054
3.768ArgArg: 3.768 ± 0.081
2.697ArgSer: 2.697 ± 0.057
2.717ArgThr: 2.717 ± 0.052
3.885ArgVal: 3.885 ± 0.058
0.499ArgTrp: 0.499 ± 0.024
2.073ArgTyr: 2.073 ± 0.055
0.0ArgXaa: 0.0 ± 0.0
Ser
6.004SerAla: 6.004 ± 0.106
0.741SerCys: 0.741 ± 0.031
3.088SerAsp: 3.088 ± 0.08
2.853SerGlu: 2.853 ± 0.059
2.554SerPhe: 2.554 ± 0.054
6.094SerGly: 6.094 ± 0.129
1.075SerHis: 1.075 ± 0.038
3.712SerIle: 3.712 ± 0.078
2.595SerLys: 2.595 ± 0.048
5.37SerLeu: 5.37 ± 0.095
1.56SerMet: 1.56 ± 0.036
2.149SerAsn: 2.149 ± 0.07
2.321SerPro: 2.321 ± 0.059
1.79SerGln: 1.79 ± 0.053
2.908SerArg: 2.908 ± 0.068
4.309SerSer: 4.309 ± 0.173
3.457SerThr: 3.457 ± 0.105
4.673SerVal: 4.673 ± 0.075
0.502SerTrp: 0.502 ± 0.024
1.948SerTyr: 1.948 ± 0.052
0.0SerXaa: 0.0 ± 0.0
Thr
6.241ThrAla: 6.241 ± 0.11
0.664ThrCys: 0.664 ± 0.033
3.185ThrAsp: 3.185 ± 0.07
2.921ThrGlu: 2.921 ± 0.06
2.442ThrPhe: 2.442 ± 0.07
5.412ThrGly: 5.412 ± 0.119
1.083ThrHis: 1.083 ± 0.039
3.441ThrIle: 3.441 ± 0.075
2.133ThrLys: 2.133 ± 0.055
5.841ThrLeu: 5.841 ± 0.098
1.28ThrMet: 1.28 ± 0.037
1.776ThrAsn: 1.776 ± 0.057
2.768ThrPro: 2.768 ± 0.072
1.851ThrGln: 1.851 ± 0.063
2.499ThrArg: 2.499 ± 0.051
3.238ThrSer: 3.238 ± 0.085
3.076ThrThr: 3.076 ± 0.097
5.552ThrVal: 5.552 ± 0.104
0.458ThrTrp: 0.458 ± 0.023
1.874ThrTyr: 1.874 ± 0.06
0.0ThrXaa: 0.0 ± 0.0
Val
6.282ValAla: 6.282 ± 0.108
1.254ValCys: 1.254 ± 0.044
3.927ValAsp: 3.927 ± 0.075
4.16ValGlu: 4.16 ± 0.082
3.392ValPhe: 3.392 ± 0.073
4.833ValGly: 4.833 ± 0.097
1.435ValHis: 1.435 ± 0.047
4.158ValIle: 4.158 ± 0.075
3.367ValLys: 3.367 ± 0.073
7.958ValLeu: 7.958 ± 0.111
1.73ValMet: 1.73 ± 0.05
2.575ValAsn: 2.575 ± 0.066
3.306ValPro: 3.306 ± 0.069
2.603ValGln: 2.603 ± 0.061
4.357ValArg: 4.357 ± 0.078
4.993ValSer: 4.993 ± 0.093
4.608ValThr: 4.608 ± 0.098
5.22ValVal: 5.22 ± 0.087
0.704ValTrp: 0.704 ± 0.03
2.289ValTyr: 2.289 ± 0.051
0.0ValXaa: 0.0 ± 0.0
Trp
0.757TrpAla: 0.757 ± 0.032
0.137TrpCys: 0.137 ± 0.012
0.491TrpAsp: 0.491 ± 0.028
0.485TrpGlu: 0.485 ± 0.026
0.398TrpPhe: 0.398 ± 0.023
0.65TrpGly: 0.65 ± 0.031
0.21TrpHis: 0.21 ± 0.015
0.451TrpIle: 0.451 ± 0.024
0.555TrpLys: 0.555 ± 0.031
0.977TrpLeu: 0.977 ± 0.035
0.304TrpMet: 0.304 ± 0.019
0.516TrpAsn: 0.516 ± 0.029
0.31TrpPro: 0.31 ± 0.018
0.419TrpGln: 0.419 ± 0.025
0.503TrpArg: 0.503 ± 0.025
0.483TrpSer: 0.483 ± 0.027
0.466TrpThr: 0.466 ± 0.027
0.573TrpVal: 0.573 ± 0.03
0.11TrpTrp: 0.11 ± 0.013
0.329TrpTyr: 0.329 ± 0.022
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.248TyrAla: 3.248 ± 0.067
0.497TyrCys: 0.497 ± 0.025
2.046TyrAsp: 2.046 ± 0.053
1.852TyrGlu: 1.852 ± 0.049
1.434TyrPhe: 1.434 ± 0.041
2.631TyrGly: 2.631 ± 0.058
0.716TyrHis: 0.716 ± 0.032
2.005TyrIle: 2.005 ± 0.057
1.415TyrLys: 1.415 ± 0.043
3.168TyrLeu: 3.168 ± 0.072
0.68TyrMet: 0.68 ± 0.03
1.38TyrAsn: 1.38 ± 0.043
1.425TyrPro: 1.425 ± 0.041
1.213TyrGln: 1.213 ± 0.042
1.942TyrArg: 1.942 ± 0.051
1.85TyrSer: 1.85 ± 0.052
2.202TyrThr: 2.202 ± 0.065
2.127TyrVal: 2.127 ± 0.05
0.313TyrTrp: 0.313 ± 0.021
1.349TyrTyr: 1.349 ± 0.051
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2663 proteins (838389 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski