Nitritalea halalkaliphila LW7

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Cyclobacteriaceae; Nitritalea; Nitritalea halalkaliphila

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3102 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I5BR73|I5BR73_9BACT Mutator family transposase (Fragment) OS=Nitritalea halalkaliphila LW7 OX=1189621 GN=A3SI_20042 PE=3 SV=1
MM1 pKa = 7.54YY2 pKa = 10.35DD3 pKa = 3.05LAGNVCEE10 pKa = 4.54LVYY13 pKa = 10.49SVRR16 pKa = 11.84NPGEE20 pKa = 3.75FVLIGGSYY28 pKa = 9.99IDD30 pKa = 4.43DD31 pKa = 3.83QEE33 pKa = 4.16TCEE36 pKa = 4.9IGRR39 pKa = 11.84ITNNVQLNWPLDD51 pKa = 3.68WPSGILGIRR60 pKa = 11.84LVKK63 pKa = 10.62DD64 pKa = 3.45LL65 pKa = 4.67

Molecular weight: 7.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I5BXU8|I5BXU8_9BACT Uncharacterized protein OS=Nitritalea halalkaliphila LW7 OX=1189621 GN=A3SI_16115 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSRR9 pKa = 11.84RR10 pKa = 11.84KK11 pKa = 9.62RR12 pKa = 11.84RR13 pKa = 11.84NKK15 pKa = 9.78HH16 pKa = 3.85GFRR19 pKa = 11.84LRR21 pKa = 11.84MSTANGRR28 pKa = 11.84RR29 pKa = 11.84VLKK32 pKa = 10.41SRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.19GRR39 pKa = 11.84HH40 pKa = 5.41KK41 pKa = 10.43LTVSSEE47 pKa = 4.01KK48 pKa = 9.88TLKK51 pKa = 10.51KK52 pKa = 10.72

Molecular weight: 6.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3102

0

3102

823449

22

1856

265.5

29.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.593 ± 0.057

0.769 ± 0.013

4.982 ± 0.032

7.048 ± 0.044

5.279 ± 0.041

7.145 ± 0.044

2.052 ± 0.024

5.945 ± 0.039

5.368 ± 0.05

10.595 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.357 ± 0.023

3.911 ± 0.045

4.305 ± 0.029

4.193 ± 0.033

5.377 ± 0.035

5.861 ± 0.033

5.027 ± 0.031

6.512 ± 0.036

1.177 ± 0.016

3.5 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski