Mycobacterium phage Giles

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Gilesvirus; Mycobacterium virus Giles

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 78 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A8WA48|A8WA48_9CAUD Uncharacterized protein OS=Mycobacterium phage Giles OX=480808 GN=60 PE=4 SV=1
MM1 pKa = 7.32TEE3 pKa = 4.22SDD5 pKa = 4.38FGMTPDD11 pKa = 3.79TADD14 pKa = 4.2DD15 pKa = 4.05NPVNTFPPNVFPLGAAVTVAYY36 pKa = 10.0GSNEE40 pKa = 3.86RR41 pKa = 11.84PFCTLGNLYY50 pKa = 10.36RR51 pKa = 11.84ILGYY55 pKa = 8.93LTGEE59 pKa = 4.16VPGADD64 pKa = 4.85DD65 pKa = 3.79INSVIAEE72 pKa = 4.25CRR74 pKa = 11.84HH75 pKa = 5.65HH76 pKa = 6.51VTHH79 pKa = 6.7QLPTDD84 pKa = 3.5MRR86 pKa = 11.84TEE88 pKa = 4.14NPPPPPGDD96 pKa = 3.7DD97 pKa = 3.45VADD100 pKa = 3.86IAWLGTVIGKK110 pKa = 9.01YY111 pKa = 9.96GPRR114 pKa = 11.84ITLTAMPGSPNNPTASDD131 pKa = 3.62TTEE134 pKa = 4.0GSTDD138 pKa = 2.96AA139 pKa = 5.22

Molecular weight: 14.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A8WA56|A8WA56_9CAUD WhiB-like protein OS=Mycobacterium phage Giles OX=480808 GN=68 PE=3 SV=1
MM1 pKa = 7.17FRR3 pKa = 11.84MRR5 pKa = 11.84IAPLRR10 pKa = 11.84NRR12 pKa = 11.84PTPRR16 pKa = 11.84RR17 pKa = 11.84LHH19 pKa = 5.77RR20 pKa = 11.84QTTGGQTMTTVVGLDD35 pKa = 3.8LSLTSAGIAVVSDD48 pKa = 3.39DD49 pKa = 3.77RR50 pKa = 11.84QADD53 pKa = 3.67RR54 pKa = 11.84MGVAWPWHH62 pKa = 5.41LRR64 pKa = 11.84CCGEE68 pKa = 4.29TGSKK72 pKa = 10.7DD73 pKa = 3.02DD74 pKa = 3.89DD75 pKa = 3.41YY76 pKa = 11.48RR77 pKa = 11.84RR78 pKa = 11.84RR79 pKa = 11.84SRR81 pKa = 11.84RR82 pKa = 11.84VRR84 pKa = 11.84RR85 pKa = 11.84QSAAVMRR92 pKa = 11.84LLEE95 pKa = 4.25PAGHH99 pKa = 7.04IDD101 pKa = 3.44LAVIEE106 pKa = 4.39GPIYY110 pKa = 10.67GGNFMPSYY118 pKa = 10.39FDD120 pKa = 3.53RR121 pKa = 11.84AALFHH126 pKa = 6.56AVYY129 pKa = 10.17GALDD133 pKa = 3.6SRR135 pKa = 11.84KK136 pKa = 9.71VPIAVISPTAGHH148 pKa = 6.44VFTTGKK154 pKa = 10.67GSLPKK159 pKa = 10.38DD160 pKa = 3.48PKK162 pKa = 10.21RR163 pKa = 11.84LKK165 pKa = 10.96GLILDD170 pKa = 4.0SVRR173 pKa = 11.84ALVPDD178 pKa = 3.23VHH180 pKa = 6.73VANDD184 pKa = 4.69DD185 pKa = 3.5IADD188 pKa = 4.03ALGLAFMGAMSLGVRR203 pKa = 11.84MPFRR207 pKa = 11.84PLRR210 pKa = 11.84RR211 pKa = 11.84HH212 pKa = 6.07HH213 pKa = 6.04EE214 pKa = 4.24QVHH217 pKa = 4.7TTTWPHH223 pKa = 5.28GRR225 pKa = 11.84PVVYY229 pKa = 10.54AA230 pKa = 4.22

Molecular weight: 25.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

78

0

78

16594

44

1359

212.7

22.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.879 ± 0.508

0.898 ± 0.176

7.25 ± 0.292

4.948 ± 0.299

2.561 ± 0.147

9.226 ± 0.589

2.001 ± 0.193

4.375 ± 0.239

2.688 ± 0.176

6.508 ± 0.277

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.218 ± 0.152

3.26 ± 0.209

6.846 ± 0.288

2.712 ± 0.224

7.888 ± 0.455

4.14 ± 0.198

6.96 ± 0.216

7.563 ± 0.324

1.832 ± 0.119

2.248 ± 0.126
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski