Amino acid dipepetide frequency for Mycobacterium phage Giles

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
24.348AlaAla: 24.348 ± 2.242
0.844AlaCys: 0.844 ± 0.248
11.511AlaAsp: 11.511 ± 1.062
8.618AlaGlu: 8.618 ± 1.271
3.375AlaPhe: 3.375 ± 0.46
10.486AlaGly: 10.486 ± 1.188
2.17AlaHis: 2.17 ± 0.501
6.087AlaIle: 6.087 ± 0.545
4.339AlaLys: 4.339 ± 0.762
9.04AlaLeu: 9.04 ± 0.772
3.737AlaMet: 3.737 ± 0.546
4.942AlaAsn: 4.942 ± 0.628
7.714AlaPro: 7.714 ± 1.155
3.616AlaGln: 3.616 ± 0.461
8.859AlaArg: 8.859 ± 0.723
5.243AlaSer: 5.243 ± 0.586
7.835AlaThr: 7.835 ± 0.688
10.426AlaVal: 10.426 ± 1.016
2.531AlaTrp: 2.531 ± 0.367
3.074AlaTyr: 3.074 ± 0.414
0.0AlaXaa: 0.0 ± 0.0
Cys
1.266CysAla: 1.266 ± 0.344
0.06CysCys: 0.06 ± 0.064
0.482CysAsp: 0.482 ± 0.18
0.422CysGlu: 0.422 ± 0.17
0.06CysPhe: 0.06 ± 0.055
1.386CysGly: 1.386 ± 0.415
0.121CysHis: 0.121 ± 0.081
0.482CysIle: 0.482 ± 0.221
0.301CysLys: 0.301 ± 0.145
0.603CysLeu: 0.603 ± 0.175
0.121CysMet: 0.121 ± 0.089
0.542CysAsn: 0.542 ± 0.276
0.603CysPro: 0.603 ± 0.187
0.0CysGln: 0.0 ± 0.0
0.603CysArg: 0.603 ± 0.183
0.241CysSer: 0.241 ± 0.158
0.844CysThr: 0.844 ± 0.265
0.422CysVal: 0.422 ± 0.194
0.301CysTrp: 0.301 ± 0.126
0.121CysTyr: 0.121 ± 0.085
0.0CysXaa: 0.0 ± 0.0
Asp
10.245AspAla: 10.245 ± 0.937
0.603AspCys: 0.603 ± 0.202
6.388AspAsp: 6.388 ± 0.916
3.978AspGlu: 3.978 ± 0.635
1.085AspPhe: 1.085 ± 0.282
6.75AspGly: 6.75 ± 0.616
1.748AspHis: 1.748 ± 0.346
4.098AspIle: 4.098 ± 0.487
0.964AspLys: 0.964 ± 0.35
3.917AspLeu: 3.917 ± 0.53
2.109AspMet: 2.109 ± 0.281
2.531AspAsn: 2.531 ± 0.401
6.629AspPro: 6.629 ± 0.631
1.748AspGln: 1.748 ± 0.328
4.821AspArg: 4.821 ± 0.653
1.386AspSer: 1.386 ± 0.288
4.821AspThr: 4.821 ± 0.656
5.725AspVal: 5.725 ± 0.697
1.446AspTrp: 1.446 ± 0.278
1.446AspTyr: 1.446 ± 0.301
0.0AspXaa: 0.0 ± 0.0
Glu
8.799GluAla: 8.799 ± 1.031
0.603GluCys: 0.603 ± 0.169
2.833GluAsp: 2.833 ± 0.509
2.17GluGlu: 2.17 ± 0.449
1.025GluPhe: 1.025 ± 0.295
3.134GluGly: 3.134 ± 0.44
1.145GluHis: 1.145 ± 0.294
1.507GluIle: 1.507 ± 0.331
0.964GluLys: 0.964 ± 0.259
3.375GluLeu: 3.375 ± 0.415
1.145GluMet: 1.145 ± 0.402
1.266GluAsn: 1.266 ± 0.275
3.737GluPro: 3.737 ± 0.788
2.109GluGln: 2.109 ± 0.354
4.701GluArg: 4.701 ± 0.558
1.567GluSer: 1.567 ± 0.232
2.652GluThr: 2.652 ± 0.384
4.641GluVal: 4.641 ± 0.505
0.844GluTrp: 0.844 ± 0.253
1.266GluTyr: 1.266 ± 0.259
0.0GluXaa: 0.0 ± 0.0
Phe
2.652PheAla: 2.652 ± 0.402
0.121PheCys: 0.121 ± 0.084
2.049PheAsp: 2.049 ± 0.367
1.446PheGlu: 1.446 ± 0.31
0.422PhePhe: 0.422 ± 0.124
3.857PheGly: 3.857 ± 0.49
0.482PheHis: 0.482 ± 0.158
1.266PheIle: 1.266 ± 0.252
0.904PheLys: 0.904 ± 0.187
1.326PheLeu: 1.326 ± 0.284
0.603PheMet: 0.603 ± 0.206
1.145PheAsn: 1.145 ± 0.212
1.446PhePro: 1.446 ± 0.326
0.422PheGln: 0.422 ± 0.166
1.808PheArg: 1.808 ± 0.404
0.844PheSer: 0.844 ± 0.216
1.326PheThr: 1.326 ± 0.277
2.471PheVal: 2.471 ± 0.382
0.422PheTrp: 0.422 ± 0.146
0.603PheTyr: 0.603 ± 0.156
0.0PheXaa: 0.0 ± 0.0
Gly
9.522GlyAla: 9.522 ± 1.006
0.603GlyCys: 0.603 ± 0.208
7.533GlyAsp: 7.533 ± 0.769
3.013GlyGlu: 3.013 ± 0.413
2.531GlyPhe: 2.531 ± 0.364
11.451GlyGly: 11.451 ± 2.236
1.808GlyHis: 1.808 ± 0.387
4.339GlyIle: 4.339 ± 0.591
2.652GlyLys: 2.652 ± 0.358
6.388GlyLeu: 6.388 ± 0.791
1.808GlyMet: 1.808 ± 0.344
3.315GlyAsn: 3.315 ± 0.502
4.279GlyPro: 4.279 ± 0.644
3.254GlyGln: 3.254 ± 0.422
7.473GlyArg: 7.473 ± 0.771
4.942GlySer: 4.942 ± 0.68
6.569GlyThr: 6.569 ± 0.73
7.232GlyVal: 7.232 ± 0.682
1.085GlyTrp: 1.085 ± 0.252
2.471GlyTyr: 2.471 ± 0.351
0.0GlyXaa: 0.0 ± 0.0
His
2.109HisAla: 2.109 ± 0.428
0.241HisCys: 0.241 ± 0.111
1.266HisAsp: 1.266 ± 0.333
0.723HisGlu: 0.723 ± 0.199
0.422HisPhe: 0.422 ± 0.175
1.748HisGly: 1.748 ± 0.347
0.422HisHis: 0.422 ± 0.178
1.145HisIle: 1.145 ± 0.288
0.482HisLys: 0.482 ± 0.184
1.145HisLeu: 1.145 ± 0.287
0.422HisMet: 0.422 ± 0.183
0.844HisAsn: 0.844 ± 0.228
1.326HisPro: 1.326 ± 0.357
0.241HisGln: 0.241 ± 0.131
2.471HisArg: 2.471 ± 0.483
0.603HisSer: 0.603 ± 0.153
1.386HisThr: 1.386 ± 0.327
2.049HisVal: 2.049 ± 0.355
0.241HisTrp: 0.241 ± 0.13
0.723HisTyr: 0.723 ± 0.195
0.0HisXaa: 0.0 ± 0.0
Ile
6.629IleAla: 6.629 ± 0.789
0.482IleCys: 0.482 ± 0.17
3.254IleAsp: 3.254 ± 0.463
2.712IleGlu: 2.712 ± 0.422
0.482IlePhe: 0.482 ± 0.167
4.038IleGly: 4.038 ± 0.474
0.844IleHis: 0.844 ± 0.225
1.748IleIle: 1.748 ± 0.438
0.663IleLys: 0.663 ± 0.189
2.471IleLeu: 2.471 ± 0.37
0.603IleMet: 0.603 ± 0.234
1.567IleAsn: 1.567 ± 0.345
3.315IlePro: 3.315 ± 0.607
1.145IleGln: 1.145 ± 0.227
4.158IleArg: 4.158 ± 0.544
1.386IleSer: 1.386 ± 0.293
2.833IleThr: 2.833 ± 0.403
3.616IleVal: 3.616 ± 0.385
0.362IleTrp: 0.362 ± 0.162
1.446IleTyr: 1.446 ± 0.339
0.0IleXaa: 0.0 ± 0.0
Lys
5.002LysAla: 5.002 ± 0.869
0.241LysCys: 0.241 ± 0.156
1.808LysAsp: 1.808 ± 0.376
0.723LysGlu: 0.723 ± 0.224
0.964LysPhe: 0.964 ± 0.234
2.17LysGly: 2.17 ± 0.399
0.482LysHis: 0.482 ± 0.174
1.205LysIle: 1.205 ± 0.382
0.542LysLys: 0.542 ± 0.185
1.507LysLeu: 1.507 ± 0.309
0.603LysMet: 0.603 ± 0.2
0.362LysAsn: 0.362 ± 0.143
2.23LysPro: 2.23 ± 0.391
0.844LysGln: 0.844 ± 0.246
2.049LysArg: 2.049 ± 0.347
1.567LysSer: 1.567 ± 0.329
1.567LysThr: 1.567 ± 0.312
1.868LysVal: 1.868 ± 0.361
0.482LysTrp: 0.482 ± 0.151
0.663LysTyr: 0.663 ± 0.189
0.0LysXaa: 0.0 ± 0.0
Leu
8.015LeuAla: 8.015 ± 0.788
0.663LeuCys: 0.663 ± 0.197
4.219LeuAsp: 4.219 ± 0.574
3.978LeuGlu: 3.978 ± 0.477
2.471LeuPhe: 2.471 ± 0.356
5.303LeuGly: 5.303 ± 0.682
1.567LeuHis: 1.567 ± 0.404
2.591LeuIle: 2.591 ± 0.337
1.446LeuLys: 1.446 ± 0.403
5.062LeuLeu: 5.062 ± 0.619
1.025LeuMet: 1.025 ± 0.191
1.748LeuAsn: 1.748 ± 0.394
4.219LeuPro: 4.219 ± 0.651
1.748LeuGln: 1.748 ± 0.413
6.027LeuArg: 6.027 ± 0.813
3.797LeuSer: 3.797 ± 0.492
4.58LeuThr: 4.58 ± 0.591
3.857LeuVal: 3.857 ± 0.506
1.326LeuTrp: 1.326 ± 0.345
1.446LeuTyr: 1.446 ± 0.269
0.0LeuXaa: 0.0 ± 0.0
Met
2.17MetAla: 2.17 ± 0.459
0.362MetCys: 0.362 ± 0.14
1.085MetAsp: 1.085 ± 0.27
0.482MetGlu: 0.482 ± 0.156
0.964MetPhe: 0.964 ± 0.246
1.748MetGly: 1.748 ± 0.267
0.422MetHis: 0.422 ± 0.143
1.145MetIle: 1.145 ± 0.244
1.205MetLys: 1.205 ± 0.301
1.507MetLeu: 1.507 ± 0.339
0.482MetMet: 0.482 ± 0.155
0.663MetAsn: 0.663 ± 0.184
1.808MetPro: 1.808 ± 0.262
0.422MetGln: 0.422 ± 0.201
1.687MetArg: 1.687 ± 0.317
1.687MetSer: 1.687 ± 0.369
2.049MetThr: 2.049 ± 0.333
1.326MetVal: 1.326 ± 0.292
0.663MetTrp: 0.663 ± 0.198
0.301MetTyr: 0.301 ± 0.139
0.0MetXaa: 0.0 ± 0.0
Asn
4.58AsnAla: 4.58 ± 0.581
0.241AsnCys: 0.241 ± 0.102
2.17AsnAsp: 2.17 ± 0.345
1.326AsnGlu: 1.326 ± 0.283
0.482AsnPhe: 0.482 ± 0.17
3.797AsnGly: 3.797 ± 0.619
0.542AsnHis: 0.542 ± 0.185
1.025AsnIle: 1.025 ± 0.337
0.362AsnLys: 0.362 ± 0.198
2.23AsnLeu: 2.23 ± 0.634
0.723AsnMet: 0.723 ± 0.212
0.723AsnAsn: 0.723 ± 0.224
3.857AsnPro: 3.857 ± 0.552
0.904AsnGln: 0.904 ± 0.244
2.531AsnArg: 2.531 ± 0.416
1.205AsnSer: 1.205 ± 0.292
2.531AsnThr: 2.531 ± 0.524
2.471AsnVal: 2.471 ± 0.4
0.482AsnTrp: 0.482 ± 0.17
0.422AsnTyr: 0.422 ± 0.152
0.0AsnXaa: 0.0 ± 0.0
Pro
9.281ProAla: 9.281 ± 1.152
0.482ProCys: 0.482 ± 0.173
6.207ProAsp: 6.207 ± 0.53
5.123ProGlu: 5.123 ± 0.977
1.808ProPhe: 1.808 ± 0.319
6.69ProGly: 6.69 ± 0.899
1.386ProHis: 1.386 ± 0.371
2.833ProIle: 2.833 ± 0.436
2.531ProLys: 2.531 ± 0.394
3.556ProLeu: 3.556 ± 0.551
1.085ProMet: 1.085 ± 0.248
2.652ProAsn: 2.652 ± 0.384
4.52ProPro: 4.52 ± 0.708
1.507ProGln: 1.507 ± 0.297
3.857ProArg: 3.857 ± 0.593
2.411ProSer: 2.411 ± 0.484
4.701ProThr: 4.701 ± 0.549
5.424ProVal: 5.424 ± 0.544
1.025ProTrp: 1.025 ± 0.22
1.386ProTyr: 1.386 ± 0.263
0.0ProXaa: 0.0 ± 0.0
Gln
3.616GlnAla: 3.616 ± 0.518
0.241GlnCys: 0.241 ± 0.178
0.482GlnAsp: 0.482 ± 0.154
0.542GlnGlu: 0.542 ± 0.168
1.266GlnPhe: 1.266 ± 0.31
1.748GlnGly: 1.748 ± 0.373
0.362GlnHis: 0.362 ± 0.14
1.205GlnIle: 1.205 ± 0.243
0.603GlnLys: 0.603 ± 0.211
2.411GlnLeu: 2.411 ± 0.558
0.723GlnMet: 0.723 ± 0.21
0.723GlnAsn: 0.723 ± 0.185
2.049GlnPro: 2.049 ± 0.34
1.446GlnGln: 1.446 ± 0.359
2.531GlnArg: 2.531 ± 0.371
1.627GlnSer: 1.627 ± 0.265
2.411GlnThr: 2.411 ± 0.421
1.446GlnVal: 1.446 ± 0.281
1.025GlnTrp: 1.025 ± 0.253
0.663GlnTyr: 0.663 ± 0.225
0.0GlnXaa: 0.0 ± 0.0
Arg
10.004ArgAla: 10.004 ± 0.893
0.482ArgCys: 0.482 ± 0.208
5.002ArgAsp: 5.002 ± 0.698
3.917ArgGlu: 3.917 ± 0.557
1.929ArgPhe: 1.929 ± 0.374
6.931ArgGly: 6.931 ± 0.6
1.989ArgHis: 1.989 ± 0.427
3.375ArgIle: 3.375 ± 0.452
2.531ArgLys: 2.531 ± 0.422
5.966ArgLeu: 5.966 ± 0.729
1.929ArgMet: 1.929 ± 0.391
3.013ArgAsn: 3.013 ± 0.436
5.123ArgPro: 5.123 ± 0.738
1.748ArgGln: 1.748 ± 0.354
8.076ArgArg: 8.076 ± 1.205
2.833ArgSer: 2.833 ± 0.46
5.183ArgThr: 5.183 ± 0.532
5.484ArgVal: 5.484 ± 0.665
1.868ArgTrp: 1.868 ± 0.402
1.507ArgTyr: 1.507 ± 0.299
0.0ArgXaa: 0.0 ± 0.0
Ser
5.906SerAla: 5.906 ± 0.625
0.301SerCys: 0.301 ± 0.139
2.652SerAsp: 2.652 ± 0.385
1.507SerGlu: 1.507 ± 0.331
1.687SerPhe: 1.687 ± 0.272
5.123SerGly: 5.123 ± 0.631
0.783SerHis: 0.783 ± 0.239
1.868SerIle: 1.868 ± 0.334
1.567SerLys: 1.567 ± 0.313
2.471SerLeu: 2.471 ± 0.423
1.145SerMet: 1.145 ± 0.254
1.145SerAsn: 1.145 ± 0.315
1.989SerPro: 1.989 ± 0.306
1.205SerGln: 1.205 ± 0.345
3.134SerArg: 3.134 ± 0.522
1.989SerSer: 1.989 ± 0.458
2.953SerThr: 2.953 ± 0.415
2.712SerVal: 2.712 ± 0.393
0.603SerTrp: 0.603 ± 0.189
0.663SerTyr: 0.663 ± 0.156
0.0SerXaa: 0.0 ± 0.0
Thr
9.221ThrAla: 9.221 ± 0.788
1.145ThrCys: 1.145 ± 0.298
4.399ThrAsp: 4.399 ± 0.526
2.531ThrGlu: 2.531 ± 0.366
1.687ThrPhe: 1.687 ± 0.314
7.051ThrGly: 7.051 ± 0.703
0.904ThrHis: 0.904 ± 0.233
3.134ThrIle: 3.134 ± 0.474
1.929ThrLys: 1.929 ± 0.35
4.761ThrLeu: 4.761 ± 0.667
1.145ThrMet: 1.145 ± 0.235
2.049ThrAsn: 2.049 ± 0.358
6.449ThrPro: 6.449 ± 0.553
1.748ThrGln: 1.748 ± 0.366
4.701ThrArg: 4.701 ± 0.531
2.17ThrSer: 2.17 ± 0.47
5.002ThrThr: 5.002 ± 0.695
6.569ThrVal: 6.569 ± 0.74
1.205ThrTrp: 1.205 ± 0.255
1.808ThrTyr: 1.808 ± 0.382
0.0ThrXaa: 0.0 ± 0.0
Val
10.245ValAla: 10.245 ± 0.909
0.663ValCys: 0.663 ± 0.199
6.207ValAsp: 6.207 ± 0.734
4.279ValGlu: 4.279 ± 0.513
1.989ValPhe: 1.989 ± 0.433
5.123ValGly: 5.123 ± 0.586
1.808ValHis: 1.808 ± 0.329
3.375ValIle: 3.375 ± 0.403
2.109ValLys: 2.109 ± 0.443
4.52ValLeu: 4.52 ± 0.579
2.049ValMet: 2.049 ± 0.325
2.35ValAsn: 2.35 ± 0.405
4.52ValPro: 4.52 ± 0.467
1.567ValGln: 1.567 ± 0.313
5.725ValArg: 5.725 ± 0.716
4.098ValSer: 4.098 ± 0.425
6.569ValThr: 6.569 ± 0.644
5.424ValVal: 5.424 ± 0.676
1.386ValTrp: 1.386 ± 0.289
1.627ValTyr: 1.627 ± 0.341
0.0ValXaa: 0.0 ± 0.0
Trp
1.989TrpAla: 1.989 ± 0.285
0.362TrpCys: 0.362 ± 0.171
1.446TrpAsp: 1.446 ± 0.281
1.145TrpGlu: 1.145 ± 0.294
0.362TrpPhe: 0.362 ± 0.129
1.025TrpGly: 1.025 ± 0.217
0.603TrpHis: 0.603 ± 0.214
0.603TrpIle: 0.603 ± 0.185
0.422TrpLys: 0.422 ± 0.162
1.507TrpLeu: 1.507 ± 0.369
0.482TrpMet: 0.482 ± 0.134
0.301TrpAsn: 0.301 ± 0.122
1.326TrpPro: 1.326 ± 0.322
0.844TrpGln: 0.844 ± 0.22
1.748TrpArg: 1.748 ± 0.257
0.904TrpSer: 0.904 ± 0.212
1.507TrpThr: 1.507 ± 0.286
0.844TrpVal: 0.844 ± 0.217
0.663TrpTrp: 0.663 ± 0.214
0.241TrpTyr: 0.241 ± 0.104
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.194TyrAla: 3.194 ± 0.512
0.241TyrCys: 0.241 ± 0.11
1.507TyrAsp: 1.507 ± 0.344
0.844TyrGlu: 0.844 ± 0.23
0.603TyrPhe: 0.603 ± 0.147
2.712TyrGly: 2.712 ± 0.445
0.422TyrHis: 0.422 ± 0.166
0.723TyrIle: 0.723 ± 0.193
0.362TyrLys: 0.362 ± 0.127
1.326TyrLeu: 1.326 ± 0.273
0.181TyrMet: 0.181 ± 0.106
0.723TyrAsn: 0.723 ± 0.164
1.326TyrPro: 1.326 ± 0.294
0.603TyrGln: 0.603 ± 0.194
1.929TyrArg: 1.929 ± 0.327
0.904TyrSer: 0.904 ± 0.203
2.29TyrThr: 2.29 ± 0.314
1.627TyrVal: 1.627 ± 0.337
0.362TyrTrp: 0.362 ± 0.151
0.603TyrTyr: 0.603 ± 0.18
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 78 proteins (16594 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski