Mycolicibacterium brumae

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Mycobacteriaceae; Mycolicibacterium

Average proteome isoelectric point is 5.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3575 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2G5PB44|A0A2G5PB44_9MYCO Pseudouridine synthase OS=Mycolicibacterium brumae OX=85968 GN=CQY22_009020 PE=4 SV=1
MM1 pKa = 7.37SEE3 pKa = 3.82AYY5 pKa = 9.62KK6 pKa = 10.34IFRR9 pKa = 11.84CQVCGFEE16 pKa = 4.24YY17 pKa = 11.0NEE19 pKa = 4.11AEE21 pKa = 4.15GWPDD25 pKa = 3.16EE26 pKa = 5.43DD27 pKa = 3.91IAPGTRR33 pKa = 11.84WADD36 pKa = 3.36IPDD39 pKa = 3.99DD40 pKa = 4.34WSCPDD45 pKa = 3.97CGAAKK50 pKa = 10.46ADD52 pKa = 3.44FDD54 pKa = 4.19MVQVGG59 pKa = 3.33

Molecular weight: 6.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2G5P5Q5|A0A2G5P5Q5_9MYCO TetR/AcrR family transcriptional regulator OS=Mycolicibacterium brumae OX=85968 GN=CQY22_016735 PE=4 SV=1
MM1 pKa = 7.69AKK3 pKa = 10.06GKK5 pKa = 8.69RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84ARR17 pKa = 11.84VHH19 pKa = 5.99GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AIVSARR37 pKa = 11.84RR38 pKa = 11.84GKK40 pKa = 10.38GRR42 pKa = 11.84RR43 pKa = 11.84SLTAA47 pKa = 3.9

Molecular weight: 5.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3575

0

3575

1151525

29

3072

322.1

34.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.571 ± 0.055

0.801 ± 0.012

6.409 ± 0.028

5.351 ± 0.039

2.948 ± 0.024

8.965 ± 0.033

2.03 ± 0.018

4.165 ± 0.026

2.184 ± 0.03

10.027 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.002 ± 0.016

2.234 ± 0.018

5.916 ± 0.037

2.875 ± 0.022

7.311 ± 0.045

5.228 ± 0.028

5.878 ± 0.03

8.558 ± 0.034

1.474 ± 0.017

2.073 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski