Culex pipiens associated Tunisia virus
Average proteome isoelectric point is 6.11
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A345GPS2|A0A345GPS2_9VIRU Capsid protein OS=Culex pipiens associated Tunisia virus OX=2079148 PE=4 SV=1
MM1 pKa = 7.62 SIFIMNLKK9 pKa = 8.31 DD10 pKa = 3.68 TQMVTYY16 pKa = 9.94 SFRR19 pKa = 11.84 FFFYY23 pKa = 10.78 LFLYY27 pKa = 9.7 FSTMSFSDD35 pKa = 3.39 GGSTNDD41 pKa = 3.27 IPKK44 pKa = 10.51 SMSSSIEE51 pKa = 3.94 VIDD54 pKa = 4.2 EE55 pKa = 4.29 NEE57 pKa = 3.86 FTYY60 pKa = 11.0 LEE62 pKa = 4.39 SPPTFHH68 pKa = 7.33 LHH70 pKa = 6.2 ISKK73 pKa = 10.59 LPNTVIEE80 pKa = 4.18 QLSKK84 pKa = 11.38 LEE86 pKa = 4.59 RR87 pKa = 11.84 DD88 pKa = 3.26 SSLNPEE94 pKa = 4.09 ILSILKK100 pKa = 9.87 FIVPNFQNGFNTLLDD115 pKa = 4.54 SIDD118 pKa = 3.75 EE119 pKa = 4.41 NNSYY123 pKa = 10.73 EE124 pKa = 4.25 YY125 pKa = 11.41 NLTNYY130 pKa = 9.94 LSEE133 pKa = 5.22 RR134 pKa = 11.84 ISSAKK139 pKa = 10.08 NQLKK143 pKa = 8.1 TAIDD147 pKa = 3.65 NLHH150 pKa = 4.98 THH152 pKa = 7.24 EE153 pKa = 4.83 ILHH156 pKa = 5.59 TLYY159 pKa = 10.76 HH160 pKa = 6.75 IGNQSIDD167 pKa = 3.5 VQNTLNEE174 pKa = 4.09 IKK176 pKa = 10.05 EE177 pKa = 4.29 VCGKK181 pKa = 10.04 LSCPDD186 pKa = 3.63 EE187 pKa = 4.54 EE188 pKa = 4.5 IKK190 pKa = 10.07 TRR192 pKa = 11.84 TSSSEE197 pKa = 3.93 IIDD200 pKa = 3.83 NLLKK204 pKa = 10.6 ILGTCKK210 pKa = 10.69 NKK212 pKa = 8.76 ITDD215 pKa = 3.19 EE216 pKa = 4.19 GTPEE220 pKa = 3.91 RR221 pKa = 11.84 EE222 pKa = 3.61 QLEE225 pKa = 4.51 RR226 pKa = 11.84 IISFSVPGSSQDD238 pKa = 3.03 KK239 pKa = 10.22 SIPSVIGEE247 pKa = 4.36 AVPHH251 pKa = 5.76 VLDD254 pKa = 4.59 FFSSKK259 pKa = 9.43 TKK261 pKa = 10.46 LAGDD265 pKa = 3.99 VVSGAINSTSGVVSDD280 pKa = 4.73 YY281 pKa = 11.38 IKK283 pKa = 11.06 GSDD286 pKa = 3.83 SKK288 pKa = 11.52 GCSLSVGDD296 pKa = 5.02 INCNCKK302 pKa = 10.66 DD303 pKa = 4.01 KK304 pKa = 11.22 DD305 pKa = 3.62 LSLPNVSGFFEE316 pKa = 4.93 DD317 pKa = 4.06 KK318 pKa = 10.44 KK319 pKa = 11.07 SSQSTPNVTSINCNCSCDD337 pKa = 3.53 YY338 pKa = 11.02 KK339 pKa = 11.19 QFLEE343 pKa = 4.14 EE344 pKa = 4.65 FKK346 pKa = 11.28 NIMDD350 pKa = 4.09 EE351 pKa = 4.05 KK352 pKa = 10.69 FEE354 pKa = 5.22 NITSVTKK361 pKa = 10.03 PRR363 pKa = 11.84 KK364 pKa = 9.31 QFLNSTKK371 pKa = 10.3 FQGLSKK377 pKa = 10.84 LIFVFDD383 pKa = 4.21 NSSQLYY389 pKa = 10.75 LNFSDD394 pKa = 5.34 LPEE397 pKa = 4.26 STKK400 pKa = 11.12 EE401 pKa = 3.9 EE402 pKa = 3.87 ILDD405 pKa = 3.65 IFDD408 pKa = 5.05 EE409 pKa = 4.68 IDD411 pKa = 3.38 FEE413 pKa = 4.95 LYY415 pKa = 10.31 EE416 pKa = 4.12 PEE418 pKa = 6.23 KK419 pKa = 10.42 IIQYY423 pKa = 10.76 SDD425 pKa = 2.89 TLYY428 pKa = 10.15 PQSSKK433 pKa = 10.54 LRR435 pKa = 11.84 RR436 pKa = 11.84 IRR438 pKa = 11.84 RR439 pKa = 11.84 AVTLKK444 pKa = 10.66 PPTPKK449 pKa = 9.2 PTINPSYY456 pKa = 10.29 NHH458 pKa = 6.25 RR459 pKa = 11.84 QIINQSKK466 pKa = 9.52 RR467 pKa = 11.84 KK468 pKa = 9.99 NIIEE472 pKa = 4.18 NPSNISRR479 pKa = 11.84 VNFITQSIEE488 pKa = 3.5 FRR490 pKa = 11.84 RR491 pKa = 11.84 VLNNLYY497 pKa = 10.37 DD498 pKa = 3.67 IEE500 pKa = 4.75 KK501 pKa = 10.34 LLVVSDD507 pKa = 4.87 DD508 pKa = 3.4 NLTYY512 pKa = 10.66 YY513 pKa = 10.79 HH514 pKa = 6.34 NQQKK518 pKa = 10.59 YY519 pKa = 5.89 KK520 pKa = 9.99 TRR522 pKa = 11.84 EE523 pKa = 3.55 EE524 pKa = 3.92 LVRR527 pKa = 11.84 FFLSNFTVDD536 pKa = 3.07 QSFYY540 pKa = 10.9 VLDD543 pKa = 3.65 NFVYY547 pKa = 10.63 DD548 pKa = 3.61 VFNVSQLVQIHH559 pKa = 5.85 KK560 pKa = 10.29 RR561 pKa = 11.84 CDD563 pKa = 3.23 VEE565 pKa = 5.51 KK566 pKa = 10.39 IFHH569 pKa = 6.23 FDD571 pKa = 3.15 EE572 pKa = 4.29 YY573 pKa = 11.58 VEE575 pKa = 4.53 YY576 pKa = 10.28 INSRR580 pKa = 11.84 IISSLKK586 pKa = 10.47 DD587 pKa = 3.35 DD588 pKa = 4.63 VISEE592 pKa = 4.29 KK593 pKa = 10.35 VLRR596 pKa = 11.84 TILNTIFKK604 pKa = 10.65 NSGIINKK611 pKa = 8.36 YY612 pKa = 8.76 FKK614 pKa = 10.58 LVKK617 pKa = 10.47 LEE619 pKa = 4.79 LEE621 pKa = 4.12 NQVTKK626 pKa = 11.08 KK627 pKa = 10.45 FISDD631 pKa = 3.6 FYY633 pKa = 11.05 IKK635 pKa = 10.55 GSPDD639 pKa = 3.28 HH640 pKa = 6.98 LISSILDD647 pKa = 3.13 IYY649 pKa = 11.38 FMDD652 pKa = 3.85 NRR654 pKa = 11.84 NLKK657 pKa = 10.0 ILLDD661 pKa = 3.57 TFRR664 pKa = 11.84 EE665 pKa = 4.05 KK666 pKa = 10.93 FLTSKK671 pKa = 11.06 DD672 pKa = 2.98 KK673 pKa = 11.5 GFIINHH679 pKa = 5.84 GFIKK683 pKa = 10.03 MSEE686 pKa = 3.78 IFQFSSNSYY695 pKa = 7.98 LTGYY699 pKa = 10.51 KK700 pKa = 8.49 ITKK703 pKa = 8.71 TNN705 pKa = 3.12
Molecular weight: 81.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.515
IPC2_protein 5.537
IPC_protein 5.55
Toseland 5.792
ProMoST 5.817
Dawson 5.703
Bjellqvist 5.766
Wikipedia 5.664
Rodwell 5.664
Grimsley 5.881
Solomon 5.703
Lehninger 5.69
Nozaki 5.919
DTASelect 6.097
Thurlkill 6.008
EMBOSS 5.97
Sillero 6.008
Patrickios 4.139
IPC_peptide 5.715
IPC2_peptide 5.995
IPC2.peptide.svr19 6.034
Protein with the highest isoelectric point:
>tr|A0A345GPS0|A0A345GPS0_9VIRU Capsid OS=Culex pipiens associated Tunisia virus OX=2079148 PE=4 SV=1
MM1 pKa = 7.74 DD2 pKa = 5.84 LEE4 pKa = 4.46 NLSHH8 pKa = 7.09 AVNTVIKK15 pKa = 10.11 NPEE18 pKa = 4.03 SLTFTVLDD26 pKa = 3.68 QILVEE31 pKa = 4.89 KK32 pKa = 10.39 IRR34 pKa = 11.84 SSNEE38 pKa = 3.56 LNSSTKK44 pKa = 10.53 KK45 pKa = 10.55 KK46 pKa = 8.13 ITKK49 pKa = 9.46 IPYY52 pKa = 10.25 SMNSEE57 pKa = 4.01 QKK59 pKa = 10.2 KK60 pKa = 10.35 ALTLMYY66 pKa = 10.34 PEE68 pKa = 5.14 FEE70 pKa = 4.23 FEE72 pKa = 4.16 YY73 pKa = 10.54 GVNFSGHH80 pKa = 4.64 VLAAASRR87 pKa = 11.84 ICEE90 pKa = 4.34 TKK92 pKa = 10.75 LIWDD96 pKa = 4.91 FVHH99 pKa = 7.11 LDD101 pKa = 3.17 DD102 pKa = 5.37 VEE104 pKa = 4.59 RR105 pKa = 11.84 IKK107 pKa = 10.98 EE108 pKa = 4.07 RR109 pKa = 11.84 IPQYY113 pKa = 10.91 DD114 pKa = 3.69 HH115 pKa = 7.08 YY116 pKa = 10.53 ITDD119 pKa = 3.03 IGGNFLTNLKK129 pKa = 9.63 CGKK132 pKa = 9.55 VKK134 pKa = 10.08 GMHH137 pKa = 5.81 SCEE140 pKa = 4.28 PTLSLYY146 pKa = 10.94 DD147 pKa = 3.34 STRR150 pKa = 11.84 RR151 pKa = 11.84 TDD153 pKa = 4.12 RR154 pKa = 11.84 IIGSLFLDD162 pKa = 3.79 PSKK165 pKa = 11.23 LNNDD169 pKa = 3.71 SNDD172 pKa = 3.14 VLTSFDD178 pKa = 3.06 KK179 pKa = 11.16 FNNFRR184 pKa = 11.84 CEE186 pKa = 4.67 RR187 pKa = 11.84 KK188 pKa = 9.12 FQNCNYY194 pKa = 9.6 TSFLGMSIHH203 pKa = 6.45 SAYY206 pKa = 10.4 DD207 pKa = 3.0 IPLQDD212 pKa = 5.81 FIDD215 pKa = 4.52 GMDD218 pKa = 4.51 NKK220 pKa = 11.01 NMMCAYY226 pKa = 9.14 LTMIFDD232 pKa = 4.68 PKK234 pKa = 10.11 MLHH237 pKa = 6.07 TDD239 pKa = 3.05 SDD241 pKa = 3.88 KK242 pKa = 11.54 LLDD245 pKa = 5.13 IEE247 pKa = 4.77 MKK249 pKa = 9.59 WSIRR253 pKa = 11.84 GEE255 pKa = 4.37 TIHH258 pKa = 6.91 FEE260 pKa = 4.38 YY261 pKa = 11.04 YY262 pKa = 10.24 NEE264 pKa = 4.25 SSLSYY269 pKa = 9.17 THH271 pKa = 6.65 NLANYY276 pKa = 9.8 LSYY279 pKa = 9.57 FTQSHH284 pKa = 6.61 MISTINKK291 pKa = 8.39 RR292 pKa = 11.84 HH293 pKa = 5.09 YY294 pKa = 9.29 TLQILKK300 pKa = 9.85 NRR302 pKa = 11.84 GYY304 pKa = 9.99 IHH306 pKa = 7.41 FIKK309 pKa = 10.23 ILYY312 pKa = 10.02 VDD314 pKa = 3.36 DD315 pKa = 3.83 TGFNRR320 pKa = 11.84 TPLVHH325 pKa = 7.08 SIPFSYY331 pKa = 9.76 YY332 pKa = 10.32 EE333 pKa = 3.76 NMIGIKK339 pKa = 10.1 YY340 pKa = 7.76 FVWTEE345 pKa = 3.55 LMKK348 pKa = 11.18 EE349 pKa = 4.05 NMSKK353 pKa = 10.8 EE354 pKa = 4.19 KK355 pKa = 10.28 IRR357 pKa = 11.84 GLLKK361 pKa = 10.45 PILLIYY367 pKa = 10.19 PEE369 pKa = 4.33 VEE371 pKa = 3.49 IEE373 pKa = 4.22 HH374 pKa = 6.17 YY375 pKa = 10.77 KK376 pKa = 10.52 SFAYY380 pKa = 9.74 KK381 pKa = 10.68 LEE383 pKa = 4.28 AGKK386 pKa = 10.38 FNPSTIFEE394 pKa = 5.03 FILSHH399 pKa = 7.19 KK400 pKa = 10.23 YY401 pKa = 10.13 RR402 pKa = 11.84 SQQDD406 pKa = 3.56 QKK408 pKa = 11.66 NVAQGKK414 pKa = 9.13 LGNVTEE420 pKa = 4.78 LFFLAHH426 pKa = 7.41 AIYY429 pKa = 10.84 LLIYY433 pKa = 9.7 KK434 pKa = 10.21 LRR436 pKa = 11.84 YY437 pKa = 7.91 TNGNVLKK444 pKa = 10.58 HH445 pKa = 6.53 LISEE449 pKa = 4.31 QLDD452 pKa = 3.34 SRR454 pKa = 11.84 KK455 pKa = 10.26 ASNINFLKK463 pKa = 10.89 KK464 pKa = 9.84 MFSKK468 pKa = 10.78 VSAKK472 pKa = 9.48 IRR474 pKa = 11.84 KK475 pKa = 9.01 AYY477 pKa = 10.23 LKK479 pKa = 10.48 LYY481 pKa = 10.8 VKK483 pKa = 10.11 FQSPPEE489 pKa = 3.88 ILEE492 pKa = 4.11 VTFSKK497 pKa = 10.01 IQEE500 pKa = 3.92 YY501 pKa = 10.58 RR502 pKa = 11.84 SFSDD506 pKa = 2.82 VKK508 pKa = 10.4 TNVKK512 pKa = 10.06 SADD515 pKa = 3.29 WVHH518 pKa = 6.36 GFTEE522 pKa = 5.32 IDD524 pKa = 3.85 PSWCIYY530 pKa = 8.89 EE531 pKa = 4.24 PKK533 pKa = 10.03 RR534 pKa = 11.84 SSVLDD539 pKa = 4.05 TILQNSDD546 pKa = 2.72 EE547 pKa = 4.49 TEE549 pKa = 3.88 IYY551 pKa = 10.39 TIDD554 pKa = 3.6 NTNKK558 pKa = 9.41 EE559 pKa = 4.45 VKK561 pKa = 10.06 SEE563 pKa = 3.83 PFSIEE568 pKa = 3.39 KK569 pKa = 8.72 HH570 pKa = 4.97 TNEE573 pKa = 4.5 HH574 pKa = 6.5 FDD576 pKa = 3.56 GAGLSIVIDD585 pKa = 3.76 EE586 pKa = 5.09 LKK588 pKa = 10.58 PGVNRR593 pKa = 11.84 HH594 pKa = 5.39 PVGSIIDD601 pKa = 3.81 CDD603 pKa = 4.43 GKK605 pKa = 11.31 CKK607 pKa = 10.35 DD608 pKa = 4.65 LEE610 pKa = 4.44 VIPNSGGGYY619 pKa = 9.51 CIYY622 pKa = 10.89 YY623 pKa = 10.94 SMLGYY628 pKa = 10.27 DD629 pKa = 4.37 DD630 pKa = 5.21 KK631 pKa = 11.42 EE632 pKa = 4.04 AAITLIKK639 pKa = 10.18 KK640 pKa = 8.72 FRR642 pKa = 11.84 SMDD645 pKa = 3.44 FNDD648 pKa = 3.59 YY649 pKa = 11.09 KK650 pKa = 11.46 DD651 pKa = 3.61 DD652 pKa = 3.78 VKK654 pKa = 11.21 IKK656 pKa = 9.2 MRR658 pKa = 11.84 KK659 pKa = 8.95 SLSLKK664 pKa = 10.19 GGLVTEE670 pKa = 4.59 LVFDD674 pKa = 4.67 LFSILEE680 pKa = 3.82 NLKK683 pKa = 10.39 IFVHH687 pKa = 5.7 TRR689 pKa = 11.84 DD690 pKa = 3.71 CKK692 pKa = 10.38 HH693 pKa = 4.99 VRR695 pKa = 11.84 FYY697 pKa = 11.73 GNEE700 pKa = 3.46 KK701 pKa = 9.97 WEE703 pKa = 4.34 DD704 pKa = 3.92 EE705 pKa = 4.17 IHH707 pKa = 6.18 VLYY710 pKa = 10.56 HH711 pKa = 6.91 DD712 pKa = 4.0 SHH714 pKa = 8.11 FEE716 pKa = 4.1 RR717 pKa = 11.84 LDD719 pKa = 3.77 LPDD722 pKa = 3.4 SKK724 pKa = 11.36 KK725 pKa = 8.47 FTKK728 pKa = 10.48 YY729 pKa = 10.97 GEE731 pKa = 4.08 GHH733 pKa = 6.22 KK734 pKa = 10.67 PIDD737 pKa = 4.22 LGLLSGKK744 pKa = 10.0 KK745 pKa = 9.12 EE746 pKa = 3.9 EE747 pKa = 4.44 GRR749 pKa = 11.84 SGVTTSKK756 pKa = 11.34 EE757 pKa = 3.57 EE758 pKa = 4.11 DD759 pKa = 3.08 KK760 pKa = 11.22 KK761 pKa = 11.4 GNSNSMVTSKK771 pKa = 10.72 KK772 pKa = 9.57 GDD774 pKa = 3.65 KK775 pKa = 10.35 IIIASKK781 pKa = 10.18 EE782 pKa = 3.77 NEE784 pKa = 4.05 LNHH787 pKa = 6.37 TGGGDD792 pKa = 3.39 TSDD795 pKa = 5.35 DD796 pKa = 3.9 LQTDD800 pKa = 3.99 DD801 pKa = 4.77 EE802 pKa = 5.71 SMTEE806 pKa = 3.99 EE807 pKa = 4.07 EE808 pKa = 4.89 NEE810 pKa = 3.93 NSSDD814 pKa = 3.89 DD815 pKa = 4.23 DD816 pKa = 4.13 VFIDD820 pKa = 5.78 KK821 pKa = 10.39 MFDD824 pKa = 3.29 KK825 pKa = 11.54 NNMSKK830 pKa = 10.96 FEE832 pKa = 4.07 TCIKK836 pKa = 10.25 FGKK839 pKa = 10.37 KK840 pKa = 9.25 LFKK843 pKa = 10.6 DD844 pKa = 3.51 NRR846 pKa = 11.84 HH847 pKa = 5.61 HH848 pKa = 7.16 SKK850 pKa = 10.71 EE851 pKa = 3.73 ILCIIRR857 pKa = 11.84 SLCDD861 pKa = 2.99 NGVFKK866 pKa = 10.6 QVYY869 pKa = 9.56 DD870 pKa = 3.6 EE871 pKa = 3.99 NLIYY875 pKa = 9.65 KK876 pKa = 7.52 TTFEE880 pKa = 5.65 DD881 pKa = 3.26 IPRR884 pKa = 11.84 VQNICYY890 pKa = 9.85 VEE892 pKa = 3.92 QDD894 pKa = 3.24 SPMKK898 pKa = 10.56 DD899 pKa = 3.17 FEE901 pKa = 5.01 KK902 pKa = 10.67 IKK904 pKa = 10.25 FLPNMFYY911 pKa = 10.75 RR912 pKa = 11.84 FVHH915 pKa = 5.85 ISKK918 pKa = 10.24 KK919 pKa = 9.7 NLVIIHH925 pKa = 5.73 VSEE928 pKa = 4.26 GCNYY932 pKa = 9.15 MNNNIFDD939 pKa = 3.55 MYY941 pKa = 10.68 YY942 pKa = 10.01 ISDD945 pKa = 3.63 HH946 pKa = 6.7 LKK948 pKa = 8.53 RR949 pKa = 11.84 TVKK952 pKa = 10.3 FFEE955 pKa = 4.18 KK956 pKa = 9.97 TGLSTVRR963 pKa = 11.84 TYY965 pKa = 11.31 PEE967 pKa = 4.39 FYY969 pKa = 9.76 EE970 pKa = 4.25 LSNKK974 pKa = 9.64 RR975 pKa = 11.84 VFVTCNQGYY984 pKa = 8.96 KK985 pKa = 10.45 DD986 pKa = 3.3 KK987 pKa = 11.31 YY988 pKa = 9.28 FYY990 pKa = 11.37 KK991 pKa = 10.78 NFLSKK996 pKa = 10.6 QFAEE1000 pKa = 3.92 RR1001 pKa = 11.84 NFINKK1006 pKa = 8.34 EE1007 pKa = 4.05 RR1008 pKa = 11.84 IEE1010 pKa = 4.11 KK1011 pKa = 10.65 VYY1013 pKa = 11.13 VFDD1016 pKa = 4.63 PYY1018 pKa = 11.86 YY1019 pKa = 10.86 KK1020 pKa = 9.01 ITPVNMRR1027 pKa = 11.84 KK1028 pKa = 10.22 YY1029 pKa = 9.73 IGLYY1033 pKa = 9.09 NEE1035 pKa = 4.08 KK1036 pKa = 9.45 TDD1038 pKa = 4.67 RR1039 pKa = 11.84 NADD1042 pKa = 3.54 DD1043 pKa = 5.3 KK1044 pKa = 11.08 IFSMVEE1050 pKa = 3.5 RR1051 pKa = 11.84 CIKK1054 pKa = 10.67 LNTVKK1059 pKa = 9.61 GTNVLLHH1066 pKa = 6.31 LHH1068 pKa = 6.23 TSTVKK1073 pKa = 10.76 LLEE1076 pKa = 3.81 QFVRR1080 pKa = 11.84 DD1081 pKa = 5.22 FIKK1084 pKa = 10.74 LPQDD1088 pKa = 3.58 NIILVIYY1095 pKa = 8.52 DD1096 pKa = 3.18 TDD1098 pKa = 3.88 YY1099 pKa = 11.32 YY1100 pKa = 10.17 KK1101 pKa = 10.63 TIHH1104 pKa = 6.76 RR1105 pKa = 11.84 KK1106 pKa = 8.32 LLKK1109 pKa = 10.33 YY1110 pKa = 10.53 SYY1112 pKa = 10.76 SVNDD1116 pKa = 3.26 NVSNFNLMEE1125 pKa = 5.13 KK1126 pKa = 10.19 ISGLDD1131 pKa = 2.93 ISPFKK1136 pKa = 10.64 TIIDD1140 pKa = 3.81 KK1141 pKa = 7.87 LTPEE1145 pKa = 5.13 DD1146 pKa = 3.53 FTSNKK1151 pKa = 9.98 RR1152 pKa = 11.84 KK1153 pKa = 9.1 FLKK1156 pKa = 10.71 LNNRR1160 pKa = 11.84 PLDD1163 pKa = 3.42 SSAVHH1168 pKa = 6.84 KK1169 pKa = 10.37 IDD1171 pKa = 5.73 EE1172 pKa = 4.15 ILKK1175 pKa = 8.78 EE1176 pKa = 4.05 YY1177 pKa = 10.74 NYY1179 pKa = 9.5 TPEE1182 pKa = 4.25 VIIDD1186 pKa = 3.7 ICANPGNFSKK1196 pKa = 10.63 ILRR1199 pKa = 11.84 CKK1201 pKa = 9.46 YY1202 pKa = 9.78 PSAILYY1208 pKa = 8.02 IHH1210 pKa = 6.97 SYY1212 pKa = 10.43 FNSKK1216 pKa = 7.68 TCDD1219 pKa = 3.13 MIDD1222 pKa = 3.39 PVFVNAKK1229 pKa = 8.25 NTILINKK1236 pKa = 9.14 RR1237 pKa = 11.84 MTNHH1241 pKa = 6.4 YY1242 pKa = 10.48 YY1243 pKa = 11.18 GDD1245 pKa = 4.27 LLDD1248 pKa = 4.62 EE1249 pKa = 4.59 SQLKK1253 pKa = 10.05 YY1254 pKa = 10.36 VDD1256 pKa = 4.55 SMLKK1260 pKa = 9.16 MADD1263 pKa = 4.49 LITSDD1268 pKa = 3.81 GCVDD1272 pKa = 4.65 AGPDD1276 pKa = 2.93 SWSNIKK1282 pKa = 8.73 LTRR1285 pKa = 11.84 NQIRR1289 pKa = 11.84 LALNHH1294 pKa = 6.4 LNIGGVFILKK1304 pKa = 9.72 VFIHH1308 pKa = 5.94 HH1309 pKa = 7.25 NIYY1312 pKa = 10.67 EE1313 pKa = 4.21 LLKK1316 pKa = 7.53 TTSRR1320 pKa = 11.84 KK1321 pKa = 7.21 FHH1323 pKa = 6.37 RR1324 pKa = 11.84 TTIIKK1329 pKa = 9.09 PHH1331 pKa = 5.99 YY1332 pKa = 9.75 SNIISPEE1339 pKa = 3.74 YY1340 pKa = 10.1 YY1341 pKa = 9.65 IYY1343 pKa = 10.72 FDD1345 pKa = 4.23 GYY1347 pKa = 9.39 SGKK1350 pKa = 9.49 IHH1352 pKa = 7.24 GEE1354 pKa = 3.98 TLSDD1358 pKa = 3.12 VDD1360 pKa = 3.81 IYY1362 pKa = 10.99 KK1363 pKa = 10.67 AYY1365 pKa = 7.73 ITYY1368 pKa = 10.36 YY1369 pKa = 10.55 KK1370 pKa = 9.95 YY1371 pKa = 9.79 IKK1373 pKa = 10.15 EE1374 pKa = 4.05 FSEE1377 pKa = 4.33 HH1378 pKa = 6.53 CNEE1381 pKa = 5.34 DD1382 pKa = 3.54 DD1383 pKa = 3.95 GLQEE1387 pKa = 3.97 GSIEE1391 pKa = 4.05 NEE1393 pKa = 4.29 SKK1395 pKa = 10.49 SGKK1398 pKa = 6.07 PTRR1401 pKa = 11.84 IKK1403 pKa = 10.64 EE1404 pKa = 4.0 KK1405 pKa = 10.25 EE1406 pKa = 3.94 KK1407 pKa = 10.75 KK1408 pKa = 10.29 FKK1410 pKa = 10.83 IKK1412 pKa = 10.32 DD1413 pKa = 3.63 SPVFSPSLQEE1423 pKa = 4.19 LYY1425 pKa = 10.88 NIYY1428 pKa = 10.86 VKK1430 pKa = 10.83 DD1431 pKa = 3.5 KK1432 pKa = 11.41 SEE1434 pKa = 4.2 TNKK1437 pKa = 8.9 TRR1439 pKa = 11.84 LLMEE1443 pKa = 4.2 MQEE1446 pKa = 4.21 SGLFDD1451 pKa = 4.09 EE1452 pKa = 5.95 NVLLKK1457 pKa = 10.41 CDD1459 pKa = 3.4 QTGCFEE1465 pKa = 4.96 DD1466 pKa = 4.73 FNSEE1470 pKa = 3.85 KK1471 pKa = 10.75 LRR1473 pKa = 11.84 VIEE1476 pKa = 4.4 TKK1478 pKa = 10.1 DD1479 pKa = 3.59 CRR1481 pKa = 11.84 IEE1483 pKa = 3.97 KK1484 pKa = 10.32 YY1485 pKa = 10.85 NNEE1488 pKa = 4.19 SYY1490 pKa = 10.98 NLKK1493 pKa = 9.54 PLSSISDD1500 pKa = 3.67 KK1501 pKa = 11.45 LPGIKK1506 pKa = 10.36 NKK1508 pKa = 10.39 DD1509 pKa = 3.77 LLKK1512 pKa = 10.47 HH1513 pKa = 5.11 WSKK1516 pKa = 11.79 NNVKK1520 pKa = 10.58 NKK1522 pKa = 9.47 FDD1524 pKa = 4.28 PYY1526 pKa = 11.15 LEE1528 pKa = 4.27 YY1529 pKa = 10.72 EE1530 pKa = 4.29 EE1531 pKa = 4.33 KK1532 pKa = 10.68 LGYY1535 pKa = 8.99 IQRR1538 pKa = 11.84 NASRR1542 pKa = 11.84 EE1543 pKa = 3.75 ILEE1546 pKa = 4.49 VYY1548 pKa = 8.36 KK1549 pKa = 8.19 TTPKK1553 pKa = 10.1 IIRR1556 pKa = 11.84 EE1557 pKa = 3.92 ILEE1560 pKa = 3.66 ILYY1563 pKa = 9.4 KK1564 pKa = 9.67 QKK1566 pKa = 10.8 KK1567 pKa = 7.86 INLVLGEE1574 pKa = 4.04 YY1575 pKa = 7.68 QVRR1578 pKa = 11.84 YY1579 pKa = 9.81 IRR1581 pKa = 11.84 LDD1583 pKa = 3.49 DD1584 pKa = 4.22 SNIGVWDD1591 pKa = 3.82 NKK1593 pKa = 9.44 IKK1595 pKa = 10.14 SWRR1598 pKa = 11.84 IKK1600 pKa = 10.48 PEE1602 pKa = 3.93 GNLKK1606 pKa = 10.13 YY1607 pKa = 10.42 DD1608 pKa = 3.48 VYY1610 pKa = 11.38 FDD1612 pKa = 3.96 GKK1614 pKa = 10.83 DD1615 pKa = 3.6 FVKK1618 pKa = 10.62 SDD1620 pKa = 3.76 NLDD1623 pKa = 3.68 STDD1626 pKa = 3.01 VRR1628 pKa = 11.84 YY1629 pKa = 10.69 LMVNNSCEE1637 pKa = 4.3 FIHH1640 pKa = 6.99 EE1641 pKa = 3.93 ISLYY1645 pKa = 10.98 DD1646 pKa = 3.47 SLKK1649 pKa = 10.89 DD1650 pKa = 3.2 INIYY1654 pKa = 11.19 DD1655 pKa = 4.44 NITNVDD1661 pKa = 3.55 VKK1663 pKa = 10.07 FVQGVPGCGKK1673 pKa = 7.14 TTYY1676 pKa = 9.82 ICSNVDD1682 pKa = 3.6 DD1683 pKa = 5.46 GDD1685 pKa = 5.13 LILASTRR1692 pKa = 11.84 EE1693 pKa = 4.2 GVKK1696 pKa = 10.53 SIEE1699 pKa = 4.22 SKK1701 pKa = 9.82 IKK1703 pKa = 9.98 KK1704 pKa = 9.65 NVVIKK1709 pKa = 9.38 TLHH1712 pKa = 6.21 SYY1714 pKa = 11.2 LMNPNKK1720 pKa = 9.91 QFEE1723 pKa = 4.73 TVWIDD1728 pKa = 3.44 EE1729 pKa = 4.26 FLMRR1733 pKa = 11.84 HH1734 pKa = 5.19 YY1735 pKa = 11.32 GEE1737 pKa = 5.38 IILVILKK1744 pKa = 8.96 SKK1746 pKa = 10.25 CKK1748 pKa = 10.12 RR1749 pKa = 11.84 IVLIGDD1755 pKa = 4.04 KK1756 pKa = 10.98 AQIPYY1761 pKa = 10.13 FNKK1764 pKa = 10.29 CPSFLLNYY1772 pKa = 10.24 SKK1774 pKa = 10.0 STDD1777 pKa = 3.73 FITPTEE1783 pKa = 4.17 NKK1785 pKa = 9.27 NVSYY1789 pKa = 10.69 RR1790 pKa = 11.84 CPLDD1794 pKa = 3.42 VAYY1797 pKa = 9.58 MFYY1800 pKa = 10.96 EE1801 pKa = 4.54 DD1802 pKa = 4.32 YY1803 pKa = 11.12 EE1804 pKa = 4.48 NGFMSVSKK1812 pKa = 10.55 FSKK1815 pKa = 9.31 TMKK1818 pKa = 9.57 TKK1820 pKa = 10.94 LINSPLDD1827 pKa = 3.2 IPKK1830 pKa = 10.4 NEE1832 pKa = 4.16 GYY1834 pKa = 10.26 KK1835 pKa = 10.62 YY1836 pKa = 10.68 LVFKK1840 pKa = 10.92 QSEE1843 pKa = 4.39 KK1844 pKa = 8.48 TTLLMEE1850 pKa = 4.63 GFKK1853 pKa = 10.84 DD1854 pKa = 3.53 VSTIGEE1860 pKa = 4.31 TQGLEE1865 pKa = 4.09 FKK1867 pKa = 10.41 KK1868 pKa = 10.6 VCLVRR1873 pKa = 11.84 LSKK1876 pKa = 10.53 IAIDD1880 pKa = 3.48 EE1881 pKa = 4.52 LYY1883 pKa = 10.88 NRR1885 pKa = 11.84 RR1886 pKa = 11.84 DD1887 pKa = 3.51 QQLVGCTRR1895 pKa = 11.84 HH1896 pKa = 6.2 RR1897 pKa = 11.84 EE1898 pKa = 3.96 EE1899 pKa = 5.41 FVYY1902 pKa = 10.73 CSIIKK1907 pKa = 10.49 DD1908 pKa = 3.73 GLFEE1912 pKa = 6.27 LINKK1916 pKa = 9.27 LPTEE1920 pKa = 4.41 DD1921 pKa = 4.12 EE1922 pKa = 4.25 IKK1924 pKa = 10.63 QKK1926 pKa = 10.91 VSITTSRR1933 pKa = 11.84 FNGAGRR1939 pKa = 11.84 RR1940 pKa = 11.84 RR1941 pKa = 11.84 RR1942 pKa = 11.84 VNMKK1946 pKa = 9.94 NLTVGDD1952 pKa = 3.94 LRR1954 pKa = 11.84 LVKK1957 pKa = 10.2 PLVINDD1963 pKa = 4.22 LKK1965 pKa = 10.96 FSEE1968 pKa = 4.57 YY1969 pKa = 10.08 FNKK1972 pKa = 9.72 VVLDD1976 pKa = 3.68 MPKK1979 pKa = 10.02 PVHH1982 pKa = 5.98 TDD1984 pKa = 2.46 KK1985 pKa = 11.19 SLQLIVDD1992 pKa = 4.42 TVNTNGGNLISNIQPKK2008 pKa = 8.2 VTYY2011 pKa = 10.22 KK2012 pKa = 10.16 IQRR2015 pKa = 11.84 PDD2017 pKa = 3.1 NQFRR2021 pKa = 11.84 CITKK2025 pKa = 10.43 GLGISYY2031 pKa = 9.88 LQDD2034 pKa = 3.3 VYY2036 pKa = 11.97 DD2037 pKa = 5.48 KK2038 pKa = 10.51 ILPGNSCFDD2047 pKa = 3.52 YY2048 pKa = 10.87 KK2049 pKa = 10.87 YY2050 pKa = 10.44 DD2051 pKa = 3.64 QLIAEE2056 pKa = 4.43 SQPIFFPYY2064 pKa = 10.25 KK2065 pKa = 10.28 NVIVDD2070 pKa = 3.42 HH2071 pKa = 6.62 SKK2073 pKa = 10.93 DD2074 pKa = 3.77 YY2075 pKa = 10.6 PLPDD2079 pKa = 4.21 HH2080 pKa = 6.92 KK2081 pKa = 10.79 PSLSPVLRR2089 pKa = 11.84 TSMAYY2094 pKa = 10.11 DD2095 pKa = 3.93 RR2096 pKa = 11.84 LPSQMDD2102 pKa = 3.6 SLIGLMKK2109 pKa = 10.4 RR2110 pKa = 11.84 NLGIPQLSGVVDD2122 pKa = 3.37 IKK2124 pKa = 11.4 YY2125 pKa = 10.32 FVDD2128 pKa = 3.39 NCVEE2132 pKa = 4.28 NFLSTYY2138 pKa = 10.44 IDD2140 pKa = 3.53 VDD2142 pKa = 3.38 KK2143 pKa = 11.55 LDD2145 pKa = 3.77 IFNMYY2150 pKa = 9.07 EE2151 pKa = 3.98 NDD2153 pKa = 3.31 MLYY2156 pKa = 10.45 PEE2158 pKa = 4.57 VNSIRR2163 pKa = 11.84 EE2164 pKa = 4.11 WLSDD2168 pKa = 3.62 QDD2170 pKa = 3.96 NKK2172 pKa = 11.17 NLGSIKK2178 pKa = 10.47 EE2179 pKa = 4.22 PFDD2182 pKa = 3.61 IFDD2185 pKa = 4.67 PNLDD2189 pKa = 3.72 LFVYY2193 pKa = 10.87 DD2194 pKa = 5.82 LMNKK2198 pKa = 7.49 QQIKK2202 pKa = 10.23 PGLTTQTPYY2211 pKa = 10.76 EE2212 pKa = 4.11 YY2213 pKa = 10.83 SAVQTIASQNKK2224 pKa = 7.5 SLNMFWCPIFRR2235 pKa = 11.84 NCKK2238 pKa = 8.15 RR2239 pKa = 11.84 RR2240 pKa = 11.84 LRR2242 pKa = 11.84 AVLKK2246 pKa = 9.53 EE2247 pKa = 3.77 NFMIFADD2254 pKa = 4.18 MSADD2258 pKa = 3.73 DD2259 pKa = 3.85 FTKK2262 pKa = 10.28 ILSDD2266 pKa = 4.08 RR2267 pKa = 11.84 FPVRR2271 pKa = 11.84 YY2272 pKa = 8.5 LQSYY2276 pKa = 9.32 KK2277 pKa = 10.62 KK2278 pKa = 10.88 LEE2280 pKa = 3.98 IDD2282 pKa = 2.61 IGKK2285 pKa = 9.68 YY2286 pKa = 10.18 DD2287 pKa = 3.66 KK2288 pKa = 10.93 SQHH2291 pKa = 5.86 RR2292 pKa = 11.84 LLFEE2296 pKa = 4.34 VEE2298 pKa = 3.75 CALYY2302 pKa = 10.24 KK2303 pKa = 10.75 KK2304 pKa = 10.64 LGIPDD2309 pKa = 3.79 EE2310 pKa = 5.78 LILKK2314 pKa = 8.54 WRR2316 pKa = 11.84 EE2317 pKa = 3.45 AHH2319 pKa = 6.83 EE2320 pKa = 4.17 FTTLKK2325 pKa = 10.59 DD2326 pKa = 3.36 RR2327 pKa = 11.84 KK2328 pKa = 9.45 NGVKK2332 pKa = 10.48 AFVEE2336 pKa = 4.37 YY2337 pKa = 10.23 QRR2339 pKa = 11.84 KK2340 pKa = 9.9 SGDD2343 pKa = 2.9 ASTFFGNTVVLMVVLCTIFDD2363 pKa = 4.06 LSDD2366 pKa = 3.35 SFGVFSGDD2374 pKa = 5.66 DD2375 pKa = 3.61 SILWMKK2381 pKa = 10.53 DD2382 pKa = 2.59 IIDD2385 pKa = 4.85 RR2386 pKa = 11.84 NDD2388 pKa = 2.75 LCANIFNLEE2397 pKa = 3.93 SKK2399 pKa = 10.29 FYY2401 pKa = 10.61 KK2402 pKa = 10.52 FEE2404 pKa = 3.91 NSYY2407 pKa = 10.72 FCSKK2411 pKa = 10.56 FLIEE2415 pKa = 6.25 VKK2417 pKa = 10.35 DD2418 pKa = 3.5 QWFLIPDD2425 pKa = 3.95 PLKK2428 pKa = 10.65 ILTKK2432 pKa = 10.46 LGRR2435 pKa = 11.84 HH2436 pKa = 6.14 NISNWDD2442 pKa = 3.36 HH2443 pKa = 7.27 LEE2445 pKa = 3.77 EE2446 pKa = 4.11 YY2447 pKa = 10.31 RR2448 pKa = 11.84 ISLCDD2453 pKa = 3.55 LLSIYY2458 pKa = 10.34 KK2459 pKa = 10.35 RR2460 pKa = 11.84 GEE2462 pKa = 3.52 IYY2464 pKa = 10.6 DD2465 pKa = 3.82 ALNVAFCEE2473 pKa = 4.19 RR2474 pKa = 11.84 YY2475 pKa = 9.2 KK2476 pKa = 11.19 VNFSIEE2482 pKa = 4.09 PLCVSILEE2490 pKa = 5.12 LISTKK2495 pKa = 11.02 NNFKK2499 pKa = 9.71 TLFYY2503 pKa = 10.53 VKK2505 pKa = 10.01 PGVKK2509 pKa = 10.34 LITDD2513 pKa = 4.37 PSHH2516 pKa = 7.05 KK2517 pKa = 10.61 KK2518 pKa = 10.32 LDD2520 pKa = 3.63
Molecular weight: 293.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.773
IPC2_protein 7.176
IPC_protein 6.985
Toseland 6.854
ProMoST 7.571
Dawson 7.688
Bjellqvist 8.024
Wikipedia 7.614
Rodwell 7.717
Grimsley 6.942
Solomon 7.688
Lehninger 7.702
Nozaki 8.185
DTASelect 7.849
Thurlkill 7.907
EMBOSS 7.922
Sillero 8.17
Patrickios 4.177
IPC_peptide 7.688
IPC2_peptide 7.073
IPC2.peptide.svr19 7.195
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
3522
137
2520
880.5
102.63
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
1.817 ± 0.476
1.76 ± 0.145
7.127 ± 0.323
6.246 ± 0.221
5.906 ± 0.607
3.833 ± 0.641
2.271 ± 0.31
8.66 ± 0.45
9.341 ± 1.354
9.284 ± 0.182
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.044 ± 0.353
6.786 ± 0.589
3.294 ± 0.161
2.612 ± 0.452
3.918 ± 0.636
8.575 ± 1.483
5.253 ± 0.353
5.48 ± 0.172
0.625 ± 0.316
5.168 ± 0.512
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here