Aromatoleum aromaticum (strain EbN1) (Azoarcus sp. (strain EbN1))

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Rhodocyclales; Rhodocyclaceae; Aromatoleum; Aromatoleum aromaticum

Average proteome isoelectric point is 6.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4483 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q5P0V1|Q5P0V1_AROAE BNR/Asp-box repeat protein OS=Aromatoleum aromaticum (strain EbN1) OX=76114 GN=ebA5171 PE=4 SV=1
MM1 pKa = 7.32SSVVGCTTTFDD12 pKa = 4.21PGWEE16 pKa = 3.6IDD18 pKa = 3.58AFGGLASLCQPMEE31 pKa = 4.59ADD33 pKa = 5.29LYY35 pKa = 11.36GCTDD39 pKa = 3.71PCWWPAQLADD49 pKa = 4.19GLNSARR55 pKa = 11.84DD56 pKa = 3.38WTDD59 pKa = 2.54GKK61 pKa = 10.79NSALRR66 pKa = 11.84DD67 pKa = 3.38WRR69 pKa = 11.84EE70 pKa = 3.84LQTLFPGDD78 pKa = 3.33

Molecular weight: 8.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q5P4Q0|Q5P4Q0_AROAE Histidine kinase OS=Aromatoleum aromaticum (strain EbN1) OX=76114 GN=ebA2830 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 9.97QPSVVRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.6RR14 pKa = 11.84THH16 pKa = 5.75GFLVRR21 pKa = 11.84MKK23 pKa = 9.7TRR25 pKa = 11.84GGRR28 pKa = 11.84AVIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.91GRR39 pKa = 11.84HH40 pKa = 4.92RR41 pKa = 11.84LAVV44 pKa = 3.37

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4483

0

4483

1390973

14

2043

310.3

33.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.271 ± 0.046

1.15 ± 0.014

5.484 ± 0.026

5.923 ± 0.03

3.634 ± 0.023

8.141 ± 0.036

2.404 ± 0.018

4.566 ± 0.025

3.133 ± 0.03

10.475 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.284 ± 0.015

2.495 ± 0.018

5.29 ± 0.028

3.366 ± 0.026

8.055 ± 0.047

5.317 ± 0.026

4.9 ± 0.025

7.49 ± 0.029

1.381 ± 0.015

2.241 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski