Colletotrichum tofieldiae
Average proteome isoelectric point is 6.48
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 12499 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A166TFK7|A0A166TFK7_9PEZI Uncharacterized protein OS=Colletotrichum tofieldiae OX=708197 GN=CT0861_03357 PE=4 SV=1
MM1 pKa = 7.56 HH2 pKa = 7.5 LTTIAALSLFTQLASAQTVVTITEE26 pKa = 4.62 GPPSPTAEE34 pKa = 4.4 PEE36 pKa = 3.94 WTSDD40 pKa = 3.49 DD41 pKa = 3.92 TFTSAVLNSTNFYY54 pKa = 10.46 RR55 pKa = 11.84 NEE57 pKa = 3.95 HH58 pKa = 5.92 NASDD62 pKa = 4.07 VTWNDD67 pKa = 2.99 TLADD71 pKa = 3.95 FAIDD75 pKa = 3.84 YY76 pKa = 10.3 LDD78 pKa = 4.88 DD79 pKa = 3.85 MSGDD83 pKa = 3.64 SCDD86 pKa = 4.21 FEE88 pKa = 5.93 HH89 pKa = 7.41 SGGPYY94 pKa = 10.49 GEE96 pKa = 4.61 NLAKK100 pKa = 10.54 GYY102 pKa = 9.83 PNTTLSIEE110 pKa = 3.93 AWGDD114 pKa = 3.18 EE115 pKa = 3.9 RR116 pKa = 11.84 DD117 pKa = 5.24 DD118 pKa = 4.04 YY119 pKa = 12.06 DD120 pKa = 3.85 FDD122 pKa = 4.63 KK123 pKa = 11.85 GEE125 pKa = 3.96 FDD127 pKa = 4.03 EE128 pKa = 4.76 DD129 pKa = 3.7 TGHH132 pKa = 6.34 FTQLVWKK139 pKa = 8.35 NTTDD143 pKa = 3.36 VGCGRR148 pKa = 11.84 KK149 pKa = 9.52 LCGDD153 pKa = 3.78 GQWYY157 pKa = 8.47 LVCEE161 pKa = 3.83 YY162 pKa = 9.57 WPRR165 pKa = 11.84 GNVVGQFVEE174 pKa = 4.73 QVQEE178 pKa = 3.92 EE179 pKa = 4.35 ALGARR184 pKa = 11.84 TAPGMLLGIVVMASFWTLSS203 pKa = 3.2
Molecular weight: 22.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.728
IPC2_protein 3.91
IPC_protein 3.897
Toseland 3.681
ProMoST 4.05
Dawson 3.884
Bjellqvist 4.037
Wikipedia 3.808
Rodwell 3.719
Grimsley 3.592
Solomon 3.872
Lehninger 3.834
Nozaki 3.999
DTASelect 4.215
Thurlkill 3.732
EMBOSS 3.821
Sillero 4.012
Patrickios 1.138
IPC_peptide 3.872
IPC2_peptide 3.999
IPC2.peptide.svr19 3.911
Protein with the highest isoelectric point:
>tr|A0A161VNC6|A0A161VNC6_9PEZI Cwf15/Cwc15 cell cycle control protein (Fragment) OS=Colletotrichum tofieldiae OX=708197 GN=CT0861_06313 PE=3 SV=1
LL1 pKa = 8.17 LEE3 pKa = 5.0 ARR5 pKa = 11.84 TTTPPLRR12 pKa = 11.84 LTSPSKK18 pKa = 10.64 RR19 pKa = 11.84 SLAVSWTSSTAWPVVVARR37 pKa = 11.84 ARR39 pKa = 11.84 SGRR42 pKa = 11.84 TVLIRR47 pKa = 11.84 PSTSSKK53 pKa = 10.54 KK54 pKa = 10.14 SFSAKK59 pKa = 9.0 VLRR62 pKa = 11.84 TTKK65 pKa = 10.55 APPSKK70 pKa = 10.17 RR71 pKa = 11.84 KK72 pKa = 8.84 MNRR75 pKa = 11.84 FPILSGASTRR85 pKa = 11.84 TPPARR90 pKa = 11.84 RR91 pKa = 11.84 FPSRR95 pKa = 11.84 IRR97 pKa = 11.84 TRR99 pKa = 11.84 SLVSKK104 pKa = 10.51 CLLAPSTTHH113 pKa = 7.19 RR114 pKa = 11.84 AASPPTIRR122 pKa = 11.84 LHH124 pKa = 7.21 DD125 pKa = 3.95 RR126 pKa = 11.84 STT128 pKa = 3.14
Molecular weight: 14.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.387
IPC2_protein 10.833
IPC_protein 12.413
Toseland 12.588
ProMoST 13.071
Dawson 12.588
Bjellqvist 12.574
Wikipedia 13.056
Rodwell 12.266
Grimsley 12.618
Solomon 13.071
Lehninger 12.983
Nozaki 12.588
DTASelect 12.574
Thurlkill 12.588
EMBOSS 13.086
Sillero 12.588
Patrickios 11.974
IPC_peptide 13.086
IPC2_peptide 12.062
IPC2.peptide.svr19 9.092
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
12499
0
12499
6353999
25
11475
508.4
56.05
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.119 ± 0.021
1.235 ± 0.009
5.673 ± 0.014
5.988 ± 0.024
3.76 ± 0.013
7.124 ± 0.02
2.344 ± 0.01
4.725 ± 0.015
4.764 ± 0.022
8.778 ± 0.021
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.169 ± 0.009
3.654 ± 0.012
6.129 ± 0.022
3.91 ± 0.017
6.016 ± 0.02
7.99 ± 0.025
6.041 ± 0.029
6.332 ± 0.017
1.536 ± 0.007
2.712 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here