Roseivirga misakiensis

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Roseivirgaceae; Roseivirga

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3764 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1E5T5T3|A0A1E5T5T3_9BACT DnaB domain-containing protein OS=Roseivirga misakiensis OX=1563681 GN=BFP71_03395 PE=4 SV=1
MM1 pKa = 7.75KK2 pKa = 10.49KK3 pKa = 10.06FLKK6 pKa = 10.13KK7 pKa = 10.92GMMGALMLSALVFTACDD24 pKa = 3.56NEE26 pKa = 4.62DD27 pKa = 4.75DD28 pKa = 4.99NGTDD32 pKa = 3.56PAGQDD37 pKa = 3.11VTVVLNEE44 pKa = 3.66VNYY47 pKa = 11.03ASGDD51 pKa = 3.42WIEE54 pKa = 4.21IFNNGSAPADD64 pKa = 3.54LSNYY68 pKa = 7.1WLCLGPGSYY77 pKa = 10.47VEE79 pKa = 5.07LSAITPQTGSVTNLLSGEE97 pKa = 4.27YY98 pKa = 10.19LVLPYY103 pKa = 10.42TMPDD107 pKa = 3.05TDD109 pKa = 4.48GGLGLYY115 pKa = 10.23SSNEE119 pKa = 4.04FTNSAAIVDD128 pKa = 4.57FVQWGSGGSARR139 pKa = 11.84EE140 pKa = 4.01DD141 pKa = 3.47VAVSAGLWTAGEE153 pKa = 4.26YY154 pKa = 10.87VPTVSSEE161 pKa = 4.05DD162 pKa = 3.26NSIIFDD168 pKa = 3.97GEE170 pKa = 4.35GNGAANWAEE179 pKa = 4.32TAQPTPGNEE188 pKa = 3.73NVLIVPEE195 pKa = 4.17PARR198 pKa = 11.84SIVISEE204 pKa = 3.97VQYY207 pKa = 11.2GNRR210 pKa = 11.84NLVEE214 pKa = 4.48LYY216 pKa = 11.1NNGEE220 pKa = 4.09VTIDD224 pKa = 3.71LSSYY228 pKa = 8.14WLCLGPGAYY237 pKa = 9.72AQIGNLTPEE246 pKa = 4.41FGSIQVEE253 pKa = 3.9AGDD256 pKa = 4.19FVVLPFNMPDD266 pKa = 3.54DD267 pKa = 4.35EE268 pKa = 6.77GGLGLYY274 pKa = 10.65SMNQFTSPDD283 pKa = 3.63AIMDD287 pKa = 4.0FVQWGATGSARR298 pKa = 11.84EE299 pKa = 4.16NVAVTAGVWTAGNFVPTVRR318 pKa = 11.84LDD320 pKa = 3.5SYY322 pKa = 11.13SIEE325 pKa = 4.1VNMEE329 pKa = 3.89SDD331 pKa = 3.77GSLASDD337 pKa = 3.05WSEE340 pKa = 3.57EE341 pKa = 4.15VNPSLGAPNNEE352 pKa = 3.81AVEE355 pKa = 4.42TTTFNVTISNMTNYY369 pKa = 10.48LNVHH373 pKa = 6.18TFTTPVGANSAGPLGVNGAQYY394 pKa = 10.53QIEE397 pKa = 4.53FKK399 pKa = 10.84AVAGTKK405 pKa = 7.43FTPVTMMGNSNDD417 pKa = 3.21WFLAPTDD424 pKa = 3.9LAGIDD429 pKa = 3.99LFPNGTALNSVDD441 pKa = 4.66IADD444 pKa = 3.87QLSLYY449 pKa = 10.87DD450 pKa = 5.26LGTEE454 pKa = 5.21ADD456 pKa = 3.61NDD458 pKa = 4.17PNNFPPAGTNVGPADD473 pKa = 3.31SDD475 pKa = 3.53ATVRR479 pKa = 11.84LVSGRR484 pKa = 11.84GTGADD489 pKa = 3.62YY490 pKa = 7.6MTAILTYY497 pKa = 10.58AAGAQNEE504 pKa = 4.53AGTFTLTITATNAPDD519 pKa = 3.64ANQAASQNNGFIITPGMVVLHH540 pKa = 6.73ALPEE544 pKa = 4.14PLFTLGSEE552 pKa = 4.05DD553 pKa = 3.25RR554 pKa = 11.84AVGLEE559 pKa = 4.21RR560 pKa = 11.84IAEE563 pKa = 4.26DD564 pKa = 3.67GMPSEE569 pKa = 4.81LYY571 pKa = 10.58DD572 pKa = 3.36WFKK575 pKa = 10.77EE576 pKa = 4.36TGSNGAPLRR585 pKa = 11.84LSSSLSVFSPGLVYY599 pKa = 10.91AFNTTTDD606 pKa = 3.68PLFTQGDD613 pKa = 3.76AARR616 pKa = 11.84AGSGIEE622 pKa = 3.96EE623 pKa = 4.23LAEE626 pKa = 4.77DD627 pKa = 4.72GNNQIAVEE635 pKa = 4.29YY636 pKa = 8.68ITNLGLPVAASNEE649 pKa = 4.35TVNIAPGEE657 pKa = 4.05DD658 pKa = 3.05LTFSIEE664 pKa = 4.02VPAGQNYY671 pKa = 10.13KK672 pKa = 10.76LGIGTMLVQTNDD684 pKa = 2.32WFVAYY689 pKa = 10.17NNSGVALWDD698 pKa = 3.72ANGAPTSGTSNSQRR712 pKa = 11.84TYY714 pKa = 10.83LYY716 pKa = 10.86DD717 pKa = 4.41AGTEE721 pKa = 3.86DD722 pKa = 4.8DD723 pKa = 3.9EE724 pKa = 5.46AVGFGANQAPRR735 pKa = 11.84QSGPNQGSADD745 pKa = 3.7GNTDD749 pKa = 2.43IRR751 pKa = 11.84RR752 pKa = 11.84VGEE755 pKa = 4.07LEE757 pKa = 3.94DD758 pKa = 3.54VQFGKK763 pKa = 11.13GLITNGPGTTYY774 pKa = 10.78LRR776 pKa = 11.84DD777 pKa = 3.29PRR779 pKa = 11.84GGYY782 pKa = 10.18NIIRR786 pKa = 11.84VDD788 pKa = 3.35IQPNN792 pKa = 3.18

Molecular weight: 83.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1E5T2V2|A0A1E5T2V2_9BACT Adenylosuccinate synthetase OS=Roseivirga misakiensis OX=1563681 GN=purA PE=3 SV=1
MM1 pKa = 7.36NGVTPTRR8 pKa = 11.84SQRR11 pKa = 11.84LSSTSGNSRR20 pKa = 11.84SAVTRR25 pKa = 11.84SAIASRR31 pKa = 11.84SQRR34 pKa = 11.84SSGRR38 pKa = 11.84VRR40 pKa = 11.84SSSSSGVSSSRR51 pKa = 11.84SSTRR55 pKa = 11.84NSTSSVASTRR65 pKa = 11.84STRR68 pKa = 11.84SRR70 pKa = 11.84RR71 pKa = 11.84TSTDD75 pKa = 2.17AYY77 pKa = 10.81RR78 pKa = 11.84FDD80 pKa = 4.15DD81 pKa = 3.46SSRR84 pKa = 11.84SRR86 pKa = 11.84RR87 pKa = 11.84SSSVRR92 pKa = 11.84ANATSGRR99 pKa = 11.84SAVSSRR105 pKa = 11.84TSRR108 pKa = 11.84SSSSRR113 pKa = 11.84SSVSRR118 pKa = 11.84SSSSRR123 pKa = 11.84SSSSRR128 pKa = 11.84PTFSRR133 pKa = 11.84SSSSRR138 pKa = 11.84SSSVRR143 pKa = 11.84SSSSRR148 pKa = 11.84SSSSRR153 pKa = 11.84SSVSRR158 pKa = 11.84SSSRR162 pKa = 11.84SSSSSRR168 pKa = 11.84SSGVRR173 pKa = 11.84SSSSRR178 pKa = 11.84SSSRR182 pKa = 11.84SSGVSRR188 pKa = 11.84SSSSRR193 pKa = 11.84SSSGSSRR200 pKa = 11.84SSSSSRR206 pKa = 11.84SGGSRR211 pKa = 11.84SSGRR215 pKa = 11.84GNN217 pKa = 3.16

Molecular weight: 22.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3764

0

3764

1367009

52

14036

363.2

40.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.856 ± 0.048

0.591 ± 0.013

5.909 ± 0.053

6.619 ± 0.052

5.211 ± 0.034

6.913 ± 0.036

1.654 ± 0.024

7.362 ± 0.04

6.607 ± 0.072

9.591 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.28 ± 0.029

5.467 ± 0.052

3.518 ± 0.022

3.507 ± 0.021

4.07 ± 0.04

6.85 ± 0.042

5.612 ± 0.101

6.509 ± 0.041

1.119 ± 0.016

3.755 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski