Manitoba virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Alpharhabdovirinae; Hapavirus; Manitoba hapavirus

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0D3R1S8|A0A0D3R1S8_9RHAB Matrix OS=Manitoba virus OX=1272949 PE=4 SV=1
MM1 pKa = 7.2EE2 pKa = 4.76TFLHH6 pKa = 6.68GGITLFCNSILVPKK20 pKa = 9.33TLLHH24 pKa = 6.31EE25 pKa = 5.33IILKK29 pKa = 8.12ITNDD33 pKa = 3.56LVHH36 pKa = 7.36DD37 pKa = 4.57CGMPPDD43 pKa = 4.09LASAISTLLLSNTLFKK59 pKa = 10.9YY60 pKa = 10.82LSDD63 pKa = 3.53GTVEE67 pKa = 4.17GTGFIQDD74 pKa = 3.35GVSYY78 pKa = 10.47KK79 pKa = 11.19GEE81 pKa = 4.4TKK83 pKa = 10.16WIDD86 pKa = 3.42QKK88 pKa = 11.46GKK90 pKa = 9.07NWNKK94 pKa = 9.92TGIHH98 pKa = 7.13DD99 pKa = 4.07LTDD102 pKa = 3.23VSGSFFEE109 pKa = 4.74YY110 pKa = 10.04FIFCSEE116 pKa = 3.82PAIFEE121 pKa = 4.15GKK123 pKa = 9.64PFEE126 pKa = 4.49EE127 pKa = 4.68LWATQDD133 pKa = 2.91SHH135 pKa = 8.27HH136 pKa = 7.4PYY138 pKa = 10.26FKK140 pKa = 10.82QKK142 pKa = 10.07MNLDD146 pKa = 3.94VYY148 pKa = 10.78SFEE151 pKa = 4.47YY152 pKa = 10.59GFNHH156 pKa = 7.39LLHH159 pKa = 7.18DD160 pKa = 4.33

Molecular weight: 18.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0D3R1H2|A0A0D3R1H2_9RHAB Phosphoprotein OS=Manitoba virus OX=1272949 PE=4 SV=1
MM1 pKa = 7.62TIMRR5 pKa = 11.84ITISHH10 pKa = 4.03QHH12 pKa = 5.26RR13 pKa = 11.84RR14 pKa = 11.84KK15 pKa = 9.39VYY17 pKa = 7.89YY18 pKa = 9.85RR19 pKa = 11.84RR20 pKa = 11.84LIMIGQSQSSIQKK33 pKa = 9.49RR34 pKa = 11.84STRR37 pKa = 11.84SWRR40 pKa = 11.84PLGILRR46 pKa = 11.84MFNQILPQKK55 pKa = 10.25RR56 pKa = 11.84KK57 pKa = 10.27YY58 pKa = 8.79LTNLINIILLYY69 pKa = 10.53LIILLQEE76 pKa = 4.32KK77 pKa = 10.27SRR79 pKa = 11.84SLSPKK84 pKa = 10.17LNPP87 pKa = 4.14

Molecular weight: 10.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11

0

11

4509

87

2109

409.9

47.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.704 ± 0.512

1.619 ± 0.177

5.611 ± 0.362

5.788 ± 0.261

4.591 ± 0.295

5.012 ± 0.286

2.573 ± 0.201

8.472 ± 0.616

7.141 ± 0.371

10.091 ± 0.755

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.595 ± 0.193

6.276 ± 0.283

4.125 ± 0.183

3.349 ± 0.305

4.524 ± 0.345

8.161 ± 0.36

4.524 ± 0.429

5.19 ± 0.317

2.129 ± 0.159

4.524 ± 0.241
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski