Streptomyces sp. SID8455

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 19049 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6B3HP39|A0A6B3HP39_9ACTN Catalase OS=Streptomyces sp. SID8455 OX=2706104 GN=G3M55_42320 PE=3 SV=1
SS1 pKa = 6.54AAEE4 pKa = 3.89AAPAPVAAPAAVPAHH19 pKa = 6.46AVTGYY24 pKa = 8.39WQNFNNGATVQTLADD39 pKa = 3.69VPDD42 pKa = 4.69AYY44 pKa = 10.84DD45 pKa = 4.0IIAVSFADD53 pKa = 3.56ATAEE57 pKa = 4.0PGEE60 pKa = 4.22ITFTLDD66 pKa = 2.87SAGLGGYY73 pKa = 7.74TDD75 pKa = 3.51EE76 pKa = 5.29QFRR79 pKa = 11.84ADD81 pKa = 4.05LAAKK85 pKa = 9.65QADD88 pKa = 4.03GKK90 pKa = 10.69SVIISVGGEE99 pKa = 3.7KK100 pKa = 10.64GAVAVNDD107 pKa = 3.77SASAQRR113 pKa = 11.84FADD116 pKa = 3.25STYY119 pKa = 11.42ALMRR123 pKa = 11.84EE124 pKa = 4.13YY125 pKa = 11.03GFDD128 pKa = 3.53GVDD131 pKa = 2.97IDD133 pKa = 6.01LEE135 pKa = 4.43NGLNSTYY142 pKa = 8.08MTEE145 pKa = 4.38ALTKK149 pKa = 9.78LHH151 pKa = 6.64EE152 pKa = 4.43KK153 pKa = 10.76AGDD156 pKa = 3.85GLVLTMAPQTIDD168 pKa = 3.19MQSPEE173 pKa = 3.83NEE175 pKa = 4.08YY176 pKa = 10.8FKK178 pKa = 10.61TALAVKK184 pKa = 10.53DD185 pKa = 3.81FLTVVNMQYY194 pKa = 11.07YY195 pKa = 10.53NSGSMLGCDD204 pKa = 3.33GQVYY208 pKa = 10.18SQGTVDD214 pKa = 5.79FLTALACIQLEE225 pKa = 4.2NGLDD229 pKa = 3.52ASQVGIGVPASPKK242 pKa = 9.95AAGGGYY248 pKa = 9.27VEE250 pKa = 4.59PSVVNDD256 pKa = 4.47ALDD259 pKa = 3.85CLTRR263 pKa = 11.84GTDD266 pKa = 3.36CGSFTPEE273 pKa = 3.24KK274 pKa = 9.66TYY276 pKa = 10.25PALRR280 pKa = 11.84GAMTWSTNWDD290 pKa = 3.68ADD292 pKa = 4.35NGNAWSNAVGPHH304 pKa = 6.36VDD306 pKa = 4.25DD307 pKa = 5.51LPP309 pKa = 5.91

Molecular weight: 32.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6B3II75|A0A6B3II75_9ACTN Uncharacterized protein (Fragment) OS=Streptomyces sp. SID8455 OX=2706104 GN=G3M55_97150 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILASRR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.36GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

19049

0

19049

3380135

18

7777

177.4

18.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.707 ± 0.035

0.756 ± 0.006

5.935 ± 0.017

5.964 ± 0.021

2.735 ± 0.013

9.645 ± 0.019

2.295 ± 0.011

3.034 ± 0.016

2.091 ± 0.017

10.542 ± 0.029

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.674 ± 0.008

1.68 ± 0.013

6.136 ± 0.02

2.663 ± 0.013

8.035 ± 0.023

4.951 ± 0.015

6.15 ± 0.018

8.484 ± 0.021

1.488 ± 0.009

2.035 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski