Amino acid dipepetide frequency for Streptomyces sp. SID8455

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	21.279AlaAla: 21.279 ± 0.112	1.044AlaCys: 1.044 ± 0.02	8.349AlaAsp: 8.349 ± 0.059	9.342AlaGlu: 9.342 ± 0.061	3.629AlaPhe: 3.629 ± 0.033	13.63AlaGly: 13.63 ± 0.078	2.869AlaHis: 2.869 ± 0.032	3.224AlaIle: 3.224 ± 0.035	2.775AlaLys: 2.775 ± 0.037	15.048AlaLeu: 15.048 ± 0.066	2.451AlaMet: 2.451 ± 0.031	1.784AlaAsn: 1.784 ± 0.025	7.45AlaPro: 7.45 ± 0.053	3.597AlaGln: 3.597 ± 0.031	10.417AlaArg: 10.417 ± 0.068	5.763AlaSer: 5.763 ± 0.04	7.155AlaThr: 7.155 ± 0.05	12.596AlaVal: 12.596 ± 0.061	1.832AlaTrp: 1.832 ± 0.023	2.836AlaTyr: 2.836 ± 0.033	0.0AlaXaa: 0.0 ± 0.0
Cys	1.079CysAla: 1.079 ± 0.017	0.093CysCys: 0.093 ± 0.006	0.44CysAsp: 0.44 ± 0.013	0.399CysGlu: 0.399 ± 0.01	0.215CysPhe: 0.215 ± 0.009	0.934CysGly: 0.934 ± 0.02	0.18CysHis: 0.18 ± 0.007	0.137CysIle: 0.137 ± 0.008	0.111CysLys: 0.111 ± 0.006	0.709CysLeu: 0.709 ± 0.015	0.106CysMet: 0.106 ± 0.006	0.127CysAsn: 0.127 ± 0.007	0.46CysPro: 0.46 ± 0.012	0.163CysGln: 0.163 ± 0.007	0.586CysArg: 0.586 ± 0.013	0.42CysSer: 0.42 ± 0.011	0.491CysThr: 0.491 ± 0.013	0.659CysVal: 0.659 ± 0.015	0.12CysTrp: 0.12 ± 0.007	0.134CysTyr: 0.134 ± 0.007	0.0CysXaa: 0.0 ± 0.0
Asp	7.761AspAla: 7.761 ± 0.051	0.382AspCys: 0.382 ± 0.012	3.483AspAsp: 3.483 ± 0.037	3.954AspGlu: 3.954 ± 0.035	1.574AspPhe: 1.574 ± 0.024	6.558AspGly: 6.558 ± 0.053	1.468AspHis: 1.468 ± 0.023	1.833AspIle: 1.833 ± 0.026	1.148AspLys: 1.148 ± 0.022	6.157AspLeu: 6.157 ± 0.046	0.743AspMet: 0.743 ± 0.015	0.886AspAsn: 0.886 ± 0.018	4.576AspPro: 4.576 ± 0.045	1.548AspGln: 1.548 ± 0.023	5.122AspArg: 5.122 ± 0.04	2.465AspSer: 2.465 ± 0.026	3.289AspThr: 3.289 ± 0.032	4.387AspVal: 4.387 ± 0.039	1.0AspTrp: 1.0 ± 0.017	1.019AspTyr: 1.019 ± 0.019	0.0AspXaa: 0.0 ± 0.0
Glu	7.969GluAla: 7.969 ± 0.057	0.363GluCys: 0.363 ± 0.011	2.976GluAsp: 2.976 ± 0.032	4.104GluGlu: 4.104 ± 0.04	1.481GluPhe: 1.481 ± 0.023	4.854GluGly: 4.854 ± 0.042	1.47GluHis: 1.47 ± 0.021	2.228GluIle: 2.228 ± 0.028	1.563GluLys: 1.563 ± 0.025	7.041GluLeu: 7.041 ± 0.053	0.86GluMet: 0.86 ± 0.016	1.051GluAsn: 1.051 ± 0.015	3.529GluPro: 3.529 ± 0.034	2.294GluGln: 2.294 ± 0.027	5.749GluArg: 5.749 ± 0.041	2.629GluSer: 2.629 ± 0.032	2.973GluThr: 2.973 ± 0.031	4.621GluVal: 4.621 ± 0.04	0.769GluTrp: 0.769 ± 0.015	1.116GluTyr: 1.116 ± 0.018	0.0GluXaa: 0.0 ± 0.0
Phe	3.794PheAla: 3.794 ± 0.035	0.263PheCys: 0.263 ± 0.01	1.966PheAsp: 1.966 ± 0.024	1.375PheGlu: 1.375 ± 0.023	0.907PhePhe: 0.907 ± 0.017	3.049PheGly: 3.049 ± 0.029	0.626PheHis: 0.626 ± 0.015	0.737PheIle: 0.737 ± 0.017	0.474PheLys: 0.474 ± 0.013	2.636PheLeu: 2.636 ± 0.03	0.383PheMet: 0.383 ± 0.012	0.54PheAsn: 0.54 ± 0.015	1.394PhePro: 1.394 ± 0.02	0.662PheGln: 0.662 ± 0.013	1.835PheArg: 1.835 ± 0.024	1.469PheSer: 1.469 ± 0.022	2.083PheThr: 2.083 ± 0.025	2.182PheVal: 2.182 ± 0.029	0.424PheTrp: 0.424 ± 0.012	0.551PheTyr: 0.551 ± 0.014	0.0PheXaa: 0.0 ± 0.0
Gly	11.566GlyAla: 11.566 ± 0.067	0.815GlyCys: 0.815 ± 0.017	5.205GlyAsp: 5.205 ± 0.04	5.641GlyGlu: 5.641 ± 0.045	2.885GlyPhe: 2.885 ± 0.03	9.047GlyGly: 9.047 ± 0.07	2.388GlyHis: 2.388 ± 0.03	3.348GlyIle: 3.348 ± 0.032	2.392GlyLys: 2.392 ± 0.027	9.771GlyLeu: 9.771 ± 0.062	1.943GlyMet: 1.943 ± 0.022	1.715GlyAsn: 1.715 ± 0.026	5.554GlyPro: 5.554 ± 0.045	2.665GlyGln: 2.665 ± 0.025	8.016GlyArg: 8.016 ± 0.055	5.426GlySer: 5.426 ± 0.041	6.48GlyThr: 6.48 ± 0.045	7.659GlyVal: 7.659 ± 0.051	1.686GlyTrp: 1.686 ± 0.024	2.248GlyTyr: 2.248 ± 0.029	0.0GlyXaa: 0.0 ± 0.0
His	2.657HisAla: 2.657 ± 0.032	0.209HisCys: 0.209 ± 0.008	1.309HisAsp: 1.309 ± 0.022	1.212HisGlu: 1.212 ± 0.019	0.64HisPhe: 0.64 ± 0.016	2.398HisGly: 2.398 ± 0.029	0.743HisHis: 0.743 ± 0.018	0.644HisIle: 0.644 ± 0.014	0.307HisLys: 0.307 ± 0.01	2.567HisLeu: 2.567 ± 0.034	0.305HisMet: 0.305 ± 0.01	0.335HisAsn: 0.335 ± 0.011	1.907HisPro: 1.907 ± 0.028	0.662HisGln: 0.662 ± 0.015	2.176HisArg: 2.176 ± 0.031	1.027HisSer: 1.027 ± 0.017	1.376HisThr: 1.376 ± 0.019	1.661HisVal: 1.661 ± 0.024	0.371HisTrp: 0.371 ± 0.009	0.441HisTyr: 0.441 ± 0.012	0.0HisXaa: 0.0 ± 0.0
Ile	4.612IleAla: 4.612 ± 0.039	0.292IleCys: 0.292 ± 0.009	2.142IleAsp: 2.142 ± 0.029	1.904IleGlu: 1.904 ± 0.026	0.685IlePhe: 0.685 ± 0.016	3.557IleGly: 3.557 ± 0.038	0.591IleHis: 0.591 ± 0.013	0.863IleIle: 0.863 ± 0.017	0.681IleLys: 0.681 ± 0.015	2.332IleLeu: 2.332 ± 0.026	0.434IleMet: 0.434 ± 0.011	0.653IleAsn: 0.653 ± 0.015	1.654IlePro: 1.654 ± 0.021	0.688IleGln: 0.688 ± 0.015	2.075IleArg: 2.075 ± 0.026	1.6IleSer: 1.6 ± 0.024	2.137IleThr: 2.137 ± 0.028	2.586IleVal: 2.586 ± 0.032	0.378IleTrp: 0.378 ± 0.009	0.474IleTyr: 0.474 ± 0.013	0.0IleXaa: 0.0 ± 0.0
Lys	2.884LysAla: 2.884 ± 0.038	0.098LysCys: 0.098 ± 0.005	1.333LysAsp: 1.333 ± 0.023	1.269LysGlu: 1.269 ± 0.022	0.457LysPhe: 0.457 ± 0.012	1.809LysGly: 1.809 ± 0.028	0.409LysHis: 0.409 ± 0.011	0.833LysIle: 0.833 ± 0.013	0.881LysLys: 0.881 ± 0.023	1.965LysLeu: 1.965 ± 0.027	0.337LysMet: 0.337 ± 0.011	0.508LysAsn: 0.508 ± 0.014	1.312LysPro: 1.312 ± 0.023	0.637LysGln: 0.637 ± 0.016	1.384LysArg: 1.384 ± 0.021	1.089LysSer: 1.089 ± 0.022	1.2LysThr: 1.2 ± 0.019	1.86LysVal: 1.86 ± 0.025	0.227LysTrp: 0.227 ± 0.009	0.417LysTyr: 0.417 ± 0.011	0.0LysXaa: 0.0 ± 0.0
Leu	15.544LeuAla: 15.544 ± 0.073	0.847LeuCys: 0.847 ± 0.016	6.892LeuAsp: 6.892 ± 0.049	4.855LeuGlu: 4.855 ± 0.049	2.699LeuPhe: 2.699 ± 0.034	9.487LeuGly: 9.487 ± 0.061	2.416LeuHis: 2.416 ± 0.032	3.244LeuIle: 3.244 ± 0.035	1.999LeuLys: 1.999 ± 0.026	11.923LeuLeu: 11.923 ± 0.073	1.714LeuMet: 1.714 ± 0.023	1.603LeuAsn: 1.603 ± 0.021	6.652LeuPro: 6.652 ± 0.045	2.101LeuGln: 2.101 ± 0.027	8.697LeuArg: 8.697 ± 0.057	5.317LeuSer: 5.317 ± 0.043	7.221LeuThr: 7.221 ± 0.052	9.059LeuVal: 9.059 ± 0.057	1.303LeuTrp: 1.303 ± 0.018	1.844LeuTyr: 1.844 ± 0.022	0.0LeuXaa: 0.0 ± 0.0
Met	2.242MetAla: 2.242 ± 0.021	0.126MetCys: 0.126 ± 0.006	0.866MetAsp: 0.866 ± 0.016	0.789MetGlu: 0.789 ± 0.016	0.44MetPhe: 0.44 ± 0.011	1.295MetGly: 1.295 ± 0.023	0.321MetHis: 0.321 ± 0.01	0.629MetIle: 0.629 ± 0.012	0.388MetLys: 0.388 ± 0.012	1.625MetLeu: 1.625 ± 0.023	0.306MetMet: 0.306 ± 0.01	0.421MetAsn: 0.421 ± 0.01	1.039MetPro: 1.039 ± 0.017	0.411MetGln: 0.411 ± 0.012	1.347MetArg: 1.347 ± 0.02	1.247MetSer: 1.247 ± 0.02	1.456MetThr: 1.456 ± 0.025	1.301MetVal: 1.301 ± 0.02	0.194MetTrp: 0.194 ± 0.007	0.303MetTyr: 0.303 ± 0.009	0.0MetXaa: 0.0 ± 0.0
Asn	2.121AsnAla: 2.121 ± 0.026	0.146AsnCys: 0.146 ± 0.007	0.908AsnAsp: 0.908 ± 0.018	0.796AsnGlu: 0.796 ± 0.015	0.466AsnPhe: 0.466 ± 0.01	1.791AsnGly: 1.791 ± 0.027	0.377AsnHis: 0.377 ± 0.012	0.644AsnIle: 0.644 ± 0.014	0.395AsnLys: 0.395 ± 0.011	1.59AsnLeu: 1.59 ± 0.024	0.288AsnMet: 0.288 ± 0.01	0.402AsnAsn: 0.402 ± 0.012	1.275AsnPro: 1.275 ± 0.021	0.509AsnGln: 0.509 ± 0.013	1.215AsnArg: 1.215 ± 0.02	0.864AsnSer: 0.864 ± 0.017	1.085AsnThr: 1.085 ± 0.019	1.267AsnVal: 1.267 ± 0.022	0.262AsnTrp: 0.262 ± 0.009	0.4AsnTyr: 0.4 ± 0.01	0.0AsnXaa: 0.0 ± 0.0
Pro	9.063ProAla: 9.063 ± 0.063	0.312ProCys: 0.312 ± 0.009	4.557ProAsp: 4.557 ± 0.038	4.674ProGlu: 4.674 ± 0.04	1.564ProPhe: 1.564 ± 0.02	7.051ProGly: 7.051 ± 0.05	1.407ProHis: 1.407 ± 0.02	1.115ProIle: 1.115 ± 0.018	1.172ProLys: 1.172 ± 0.02	5.615ProLeu: 5.615 ± 0.045	0.971ProMet: 0.971 ± 0.016	0.817ProAsn: 0.817 ± 0.017	3.52ProPro: 3.52 ± 0.042	1.678ProGln: 1.678 ± 0.024	3.898ProArg: 3.898 ± 0.036	2.992ProSer: 2.992 ± 0.033	3.0ProThr: 3.0 ± 0.036	5.654ProVal: 5.654 ± 0.041	0.907ProTrp: 0.907 ± 0.02	1.397ProTyr: 1.397 ± 0.021	0.0ProXaa: 0.0 ± 0.0
Gln	3.616GlnAla: 3.616 ± 0.034	0.153GlnCys: 0.153 ± 0.006	1.404GlnAsp: 1.404 ± 0.021	1.573GlnGlu: 1.573 ± 0.021	0.66GlnPhe: 0.66 ± 0.015	2.283GlnGly: 2.283 ± 0.026	0.632GlnHis: 0.632 ± 0.012	1.043GlnIle: 1.043 ± 0.017	0.6GlnLys: 0.6 ± 0.013	2.92GlnLeu: 2.92 ± 0.034	0.455GlnMet: 0.455 ± 0.012	0.504GlnAsn: 0.504 ± 0.013	1.555GlnPro: 1.555 ± 0.023	1.188GlnGln: 1.188 ± 0.023	2.289GlnArg: 2.289 ± 0.028	1.179GlnSer: 1.179 ± 0.018	1.238GlnThr: 1.238 ± 0.018	2.331GlnVal: 2.331 ± 0.025	0.438GlnTrp: 0.438 ± 0.012	0.568GlnTyr: 0.568 ± 0.014	0.0GlnXaa: 0.0 ± 0.0
Arg	10.043ArgAla: 10.043 ± 0.064	0.585ArgCys: 0.585 ± 0.012	4.296ArgAsp: 4.296 ± 0.047	4.981ArgGlu: 4.981 ± 0.041	2.346ArgPhe: 2.346 ± 0.028	5.898ArgGly: 5.898 ± 0.048	2.092ArgHis: 2.092 ± 0.026	3.163ArgIle: 3.163 ± 0.03	1.604ArgLys: 1.604 ± 0.028	8.928ArgLeu: 8.928 ± 0.056	1.558ArgMet: 1.558 ± 0.02	1.368ArgAsn: 1.368 ± 0.022	5.047ArgPro: 5.047 ± 0.041	2.153ArgGln: 2.153 ± 0.023	7.585ArgArg: 7.585 ± 0.056	4.116ArgSer: 4.116 ± 0.033	5.611ArgThr: 5.611 ± 0.047	5.873ArgVal: 5.873 ± 0.045	1.339ArgTrp: 1.339 ± 0.021	1.763ArgTyr: 1.763 ± 0.02	0.0ArgXaa: 0.0 ± 0.0
Ser	6.725SerAla: 6.725 ± 0.043	0.398SerCys: 0.398 ± 0.011	2.556SerAsp: 2.556 ± 0.026	2.378SerGlu: 2.378 ± 0.029	1.557SerPhe: 1.557 ± 0.021	5.913SerGly: 5.913 ± 0.049	1.017SerHis: 1.017 ± 0.017	1.317SerIle: 1.317 ± 0.019	0.95SerLys: 0.95 ± 0.019	4.957SerLeu: 4.957 ± 0.043	0.958SerMet: 0.958 ± 0.015	0.815SerAsn: 0.815 ± 0.019	3.101SerPro: 3.101 ± 0.033	1.208SerGln: 1.208 ± 0.019	3.584SerArg: 3.584 ± 0.036	2.716SerSer: 2.716 ± 0.035	3.016SerThr: 3.016 ± 0.036	4.275SerVal: 4.275 ± 0.042	0.888SerTrp: 0.888 ± 0.016	1.185SerTyr: 1.185 ± 0.021	0.0SerXaa: 0.0 ± 0.0
Thr	9.0ThrAla: 9.0 ± 0.055	0.4ThrCys: 0.4 ± 0.012	3.496ThrAsp: 3.496 ± 0.035	3.462ThrGlu: 3.462 ± 0.036	1.636ThrPhe: 1.636 ± 0.021	7.088ThrGly: 7.088 ± 0.055	1.172ThrHis: 1.172 ± 0.02	1.566ThrIle: 1.566 ± 0.024	1.048ThrLys: 1.048 ± 0.019	5.962ThrLeu: 5.962 ± 0.052	0.927ThrMet: 0.927 ± 0.016	0.927ThrAsn: 0.927 ± 0.017	4.146ThrPro: 4.146 ± 0.038	1.258ThrGln: 1.258 ± 0.018	3.934ThrArg: 3.934 ± 0.035	3.034ThrSer: 3.034 ± 0.03	3.884ThrThr: 3.884 ± 0.04	6.302ThrVal: 6.302 ± 0.046	0.905ThrTrp: 0.905 ± 0.016	1.353ThrTyr: 1.353 ± 0.021	0.0ThrXaa: 0.0 ± 0.0
Val	10.678ValAla: 10.678 ± 0.057	0.741ValCys: 0.741 ± 0.016	4.931ValAsp: 4.931 ± 0.035	4.881ValGlu: 4.881 ± 0.044	2.374ValPhe: 2.374 ± 0.026	6.48ValGly: 6.48 ± 0.048	2.053ValHis: 2.053 ± 0.024	2.796ValIle: 2.796 ± 0.033	1.699ValLys: 1.699 ± 0.027	9.906ValLeu: 9.906 ± 0.068	1.478ValMet: 1.478 ± 0.021	1.593ValAsn: 1.593 ± 0.023	5.345ValPro: 5.345 ± 0.044	2.034ValGln: 2.034 ± 0.024	7.318ValArg: 7.318 ± 0.048	4.317ValSer: 4.317 ± 0.039	5.576ValThr: 5.576 ± 0.042	7.964ValVal: 7.964 ± 0.056	1.138ValTrp: 1.138 ± 0.02	1.535ValTyr: 1.535 ± 0.021	0.0ValXaa: 0.0 ± 0.0
Trp	1.668TrpAla: 1.668 ± 0.022	0.138TrpCys: 0.138 ± 0.006	0.799TrpAsp: 0.799 ± 0.016	0.797TrpGlu: 0.797 ± 0.016	0.524TrpPhe: 0.524 ± 0.014	1.065TrpGly: 1.065 ± 0.02	0.337TrpHis: 0.337 ± 0.009	0.518TrpIle: 0.518 ± 0.012	0.362TrpLys: 0.362 ± 0.009	1.708TrpLeu: 1.708 ± 0.023	0.279TrpMet: 0.279 ± 0.01	0.373TrpAsn: 0.373 ± 0.01	0.783TrpPro: 0.783 ± 0.017	0.585TrpGln: 0.585 ± 0.013	1.32TrpArg: 1.32 ± 0.02	0.934TrpSer: 0.934 ± 0.017	1.022TrpThr: 1.022 ± 0.017	0.978TrpVal: 0.978 ± 0.018	0.34TrpTrp: 0.34 ± 0.011	0.349TrpTyr: 0.349 ± 0.011	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.77TyrAla: 2.77 ± 0.032	0.164TyrCys: 0.164 ± 0.007	1.444TyrAsp: 1.444 ± 0.024	1.255TyrGlu: 1.255 ± 0.021	0.611TyrPhe: 0.611 ± 0.013	2.262TyrGly: 2.262 ± 0.026	0.379TyrHis: 0.379 ± 0.012	0.456TyrIle: 0.456 ± 0.011	0.36TyrLys: 0.36 ± 0.01	2.055TyrLeu: 2.055 ± 0.024	0.247TyrMet: 0.247 ± 0.007	0.378TyrAsn: 0.378 ± 0.011	1.067TyrPro: 1.067 ± 0.02	0.587TyrGln: 0.587 ± 0.014	1.801TyrArg: 1.801 ± 0.023	0.909TyrSer: 0.909 ± 0.019	1.21TyrThr: 1.21 ± 0.02	1.622TyrVal: 1.622 ± 0.023	0.358TyrTrp: 0.358 ± 0.01	0.416TyrTyr: 0.416 ± 0.013	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 19049 proteins (3380135 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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